Carolina Fish Protein Fingerprinting on Polyacrylamide Gels

PAGE, Analyzing proteins & Evolution

Timeline:Monday 1/28: LectureTuesday 1/29: Pre-Lab Quiz, Run Gel, Stain

and De-stain (someone plan to stay late)Thursday: Analyze Gels

Prep. For labdilute stains and buffersice?boiling water bath

PAGE: Polyacrylamide Gel Electrophoresis

Separates Proteins by size or chargeUsed to estimate size of DNA/RNA fragmentsDNA loaded into the well will run positive through the pores

of the Polyacrylamide GelsSorts DNA according to length

Smaller strands will run longer because they can fit through more pores in the gels than bigger strands of DNA

The Gel can be seen under UV light or stained with Ethidium Bromide to be seen with the human eye

Analyzing Proteins:PAGE can provide:

Size of DNA molecules after restriction enzyme digestion

Genetic FingerprintingDesired protein was properly isolatedBase Pair SizeWhether food is Genetically ModifiedWhat the Relationship is between species (through shared base pairs)

EvolutionPAGE Analysis:

Help clarify the connections between species

how they are relatedEvolution of fish species

The genes that connect them The differences in genetic material due to surroundings

Polyacrylamide gelsTutorial 23 and 25 from disc.