p3_biology Ting 5 2016

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4551/3 SULIT

SULIT 2

Answer all questions.

Jawab semua soalan.

Question 1

Soalan 1

[33 marks]

[33 markah]

1. An experiment was carried out to study the effect of enzyme concentration on the activity of

amylase on starch.

Three test tubes ! " and # were filled with 2ml of $.%&! '.$& and '.%& of amylase

respectively. 2ml of '& starch suspension was added into each test tube and the contents were

stirred.

(very 2 minutes a drop of the mixture from each test tube was mixed with a drop of iodine

solution on a white tile.

All test tubes are immersed in a water bath at temperature of )*+, durin- the experiment as

shown in dia-ram below.

Table shows the results of the experiment.

Satu eksperimen telah dijalankan untuk mengkaji kesan kepekatan enzim ke atas aktiviti

amilase ke atas kanji.

Tiga tabung uji P, Q dan R diisi dengan 2ml larutan 0.5%, .0% dan .5% amilase masing!

masing. 2ml ampaian kanji % dimasukkan ke dalam setiap tabung uji dan "ampuran

dika"au..

Setiap 2 minit setitik "ampuran daripada setiap tabung uji di"ampurkan ke dalam setitis

larutan i#din di atas jubin putih.

$esemua tabung didih direndam dalam kukus air pada suhu &'( sepanjang masa

eksperimen seperti )ang ditunjukkan dalam rajah di ba*ah.

+adual menunjukkan keputusan eksperimen.

" #

.ater bath )* , $ukus air %& (

Amylase / starch

,milase - kanji

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Test tube

Tabung

uji

The colour of iodine solution startin- from

minute $

arna larutan i#din bermula dari minit 0

Time ta0en for the starch to be

hydrolysed completely startin-

from minute $ 1min

/asa diambil bagi kanjidihidr#lisiskan dengan lengkap

bermula dari minit 0 min1

3ar0 blue

iru tua

4ellow 1no

chan-e

$uning

tiada

perubahan1

60

30

45 15

1050

40

35 25

20

555Time ta0en

5

/asa diambil

minutes

minit

"

60

30

45 15

1050

40

35 25

20

555Time ta0en

5

/asa diambil

minutes

minit

#

60

30

45 15

1050

40

35 25

20

555

Time ta0en

5

/asa diambil

minutes

minit

1a #ecord the time ta0en for the starch to be hydrolysed completely in the boxes provided

in table above. Rek#d masa )ang diambil bagi kanji dihidr#lisiskan dengan lengkap di dalam k#tak

dalam jadual di atas.

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4551/3 SULIT

SULIT 6

[ 3 marks/ markah]

1b 1i 7ased on the table! state two observations that can be made from the experiment.

erdasarkan jadual, n)atakan dua pemerhatian )ang dapat dibuat dalameksperimen ini.

8bservation '9

Pemerhatian 3

8bservation 29 Pemerhatian 23

[ 3 marks/ markah]

1ii State the inference for each observation made in 1b1i.

4)atakan inerens terhadap setiap pemerhatian )ang dibuat dalam b1i1.

Inference for observation '9

6nerens terhadap pemerhatian 3

Inference for observation 29

6nerens terhadap pemerhatian 23

[ 3 marks/ markah]

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1c ,omplete table below based on the experiment.

Len-0ap0an :adual di bawah berdasar0an e0sperimen ini.

Variable Pembolehubah

Method to handle the variableCara mengendalikan pembolehubah

;anipulated variable

Pemb#lehubah dimanipulasikan

.................................................

..................................................

..................................................

................................................

................................................

................................................

#espondin- variable

Pemb#lehubah bergerak balas

..................................................

..................................................

..................................................

.................................................

................................................

................................................

,onstant variable

Pemb#lehubah dimalarkan

....................................................

..................................................

...................................................

...............................................

................................................

.................................................

[ 3 marks/ markah]

1d State the hypothesis for this experiment.

4)atakan hip#tesis bagi eksperimen ini.

[ 3 marks/ markah]

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1e ,onstruct a table to record all the data collected in this experiment.

4our table should have the followin- data.

= (nzyme concentration

= The time ta0en for starch to be hydrolysed completely! t 1minute= The rate of enzyme amylase activity! '>t 1minute='

ina satu jadual untuk merek#dkan semua data )ang dikutip dalam eksperimen ini.

+adual anda hendaklah mengandungi data!data berikut3

! $epekatan enzim

! /asa )ang diambil bagi kanji dihidr#lisiskan dengan lengkap, minit1

! $adar aktiviti enzim amilase, 7t minit ! 1

[ 3 marks/ markah]

1f lot a -raph of the rate of enzyme amylase activity a-ainst enzyme concentration on-raph paper.

Pl#tkan gra kadar aktiviti enzim amilase mela*an kepekatan enzim pada kertas gra.

[ 3 marks/ markah]

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kesan p; ke atas aktiviti enzim.

= Sodium hydroxide solution

<arutan natrium hidr#ksida

= ?ydrochloric acid sid hidr#kl#rik

= 3istilled water

ir suling

,omplete the followin- table to classify the materials accordin- to the type of medium.

<engkapkan jadual berikut untuk mengelaskan bahan!bahan tersebut berdasarkan jenis

medium.

;aterial

ahan

Type of medium

+enis medium

[ 3 marks/ markah]

Question 2Soalan 2

[17 marks]

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4551/3 SULIT

SULIT

[17 markah]

A housewife made fruit pickles using unripe mango !uring thepreparation" she placed the mango slices in water and later placed them insugar solution

Seorang suri rumah telah membuat jeruk buah menggunakan mangamuda. Semasa menyediakan jeruk tersebut, suri rumah itu telah meredam

potongan buah manga dalam air dan kemudian potongan mangga itudirendam pula dalam larutan gula.

#hen the mango slices were in water" it was found that" the slices $ecameturgid and their si%es increased &ut when the' were paced in the sugarsolution" the slices $ecame soft and shrunken

Semasa potongan mangga direndam dalam air, didapati potongan manggaitu menjadi segah dan saiznya bertambah. etapi apabila potongan

mangga itu direndam dalam larutan gula, ia menjadi lembik danmenge!ut.

!esign a la$orator' e(periment to stud' the e)ect of di)erentconcentration of sucrose on the mass of mango slice"ekabentuk satu eksperimen dalam makmal untuk mengkaji kesankepekatan sukrosa berbeza terhadap jisim potongan mangga.

*he planning of 'our e(periment must include the following aspects +#eran!angan eksperimen anda hendaklah meliputi aspek$aspek berikut %

• ,ro$lem statement

• #ernyataan masalah

• -'pothesis

• &ipotesis

• .aria$les

• #embolehubah

•

/ist of apparatus and materials• Senarai radas dan bahan

• 0(perimental procedure or method

• #rosedur atau kaedah eksperimen

• !ata presentation

• #ersembahan data

[17 marksmarkah]

!"T#S S$#L#% T#M#T

3isedia0an oleh! 3isema0 oleh! 3isah0an oleh!

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1n Ceronica a>p Drancis 1n ;aznah bt Ahmad 1n an Sepiah bt an Eain

F 7iolo-i GF Sains dan ;atemati0 F entadbiran

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p3_biology Ting 5 2016