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Osteogenic differentiation and mineralization in fibre-reinforced tubular scaffolds: theoretical study and
experimental evidences
by Vincenzo Guarino, Francesco Urciuolo, Marco A. Alvarez-Perez, Benedetto Mele, Paolo A. Netti, and Luigi Ambrosio
InterfaceVolume 9(74):2201-2212
September 7, 2012
©2012 by The Royal Society
Two step preparation scheme of PCL/PLA tubes (adapted from Guarino et al. [21]): (a) filament winding technology to integrate a PLA long fibre to the porous PCL matrix to obtain the tubular
structure (left); (b) phase inversion and salt leaching technique t...
Vincenzo Guarino et al. J. R. Soc. Interface 2012;9:2201-2212
©2012 by The Royal Society
Effect of polymer/solvent ratio on structural properties of PCL/PLA tubes.
Vincenzo Guarino et al. J. R. Soc. Interface 2012;9:2201-2212
©2012 by The Royal Society
Effect of fibre cycle on structural properties of PCL/PLA tubes.
Vincenzo Guarino et al. J. R. Soc. Interface 2012;9:2201-2212
©2012 by The Royal Society
Modelling based on computational fluid dynamics (CFD) simulation.
Vincenzo Guarino et al. J. R. Soc. Interface 2012;9:2201-2212
©2012 by The Royal Society
Distribution of the wall shear stresses obtained by imposing three different flow rates: (a) 0.005, (b) 0.05 and (c) 0.5 ml min–1.
Vincenzo Guarino et al. J. R. Soc. Interface 2012;9:2201-2212
©2012 by The Royal Society
Radial perfusion bioreactor system.
Vincenzo Guarino et al. J. R. Soc. Interface 2012;9:2201-2212
©2012 by The Royal Society
Gene expression by RT-PCR of relevant bone-related makers (ALP (black), OC (light grey), OPN (dark grey)) and quantitative estimation of signal.
Vincenzo Guarino et al. J. R. Soc. Interface 2012;9:2201-2212
©2012 by The Royal Society
Mineral phase detection: Alizarin Red staining of PCL/PLA scaffolds as a function of perfusion flow rate.
Vincenzo Guarino et al. J. R. Soc. Interface 2012;9:2201-2212
©2012 by The Royal Society
