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Organic Compounds in Food Lab. Purpose : Test samples of food for the presence of organic molecules using 4 indicators. Procedure: Materials : 7 food sources, beakers, pipettes, and 4 indicators: Benedict’s Solution – detects glucose (simple sugar) - PowerPoint PPT Presentation
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Organic Compounds in Food Lab
Purpose: Test samples of food for the presence of organic molecules using 4 indicators.
Procedure: Materials: 7 food sources, beakers,
pipettes, and 4 indicators: Benedict’s Solution – detects
glucose (simple sugar) Lugol’s Iodine Solution – detects
Starch (complex sugar) Biuret Solution – detects proteins Sudan IV – detects lipids (fats)
Procedures: At lab station
Procedures – At lab station 1. Demo standards and record results in Data Table 1.
2. Make predictions.
3. Place approximately 5 mL of the first food substance in each of the 4 test tubes.
Conduct the 4 indicator tests as shown in the demo: 5 drops of each substance. Benedicts – hot
water 5 min
Record your results in Data Table 2.
Wash 4 test tubes and repeat with sample 2.
Repeat the above steps for all 7 substances.
Data Table 1: Standards Test
Substance
Glucose Lipid Starch Protein
Indicator Used
Color changes
from _____ to _______
Other Observati
on
Data Table 2: Organic Compounds in Food
Food Lipid Test Carbohydrate Test Protein Test
Sudan IV Color
Lipids Present
Iodine Color
Starches
Present
Benedict Color
Glucose Present
Biuret Color
Proteins Present
Honey
Egg White
Potato
Milk
Apple Juice
Veg. Oil
Corn
Conclusion
1 to 2 paragraphs. Written in 3rd person (command) form, not in 1st person.
Discuss your Results: Which substances contain lipids, simple
sugars, starch, lipids and proteins? How was this determined? Describe the
4 tests and the qualitative results. Discuss any surprising or expected
results. Review your original predictions.
Procedures 1. Place approximately 5 mL of the first food
substance in each of the 4 test tubes.
2. Conduct the 4 indicator tests as shown in the demo: 5 drops of each substance. Benedicts should sit in water bath for 5
min.
3. Record your results in Data Table 2.
4. Wash 4 test tubes in the sink and repeat with the remaining 6 samples.