ORF Homology

ORF Start1

ORF Stop

TATA box

Polyadenylation Signal

Homology of predicted 1° Sequence2

Notes

UL 23 (TK) 1059 73 1118 73 Herpesvirus TK Polyadenylation seq. is noncanonical (AATTAA)

UL 24 1008 1949 1053 1857 or 1895 or 50403

Herpesvirus UL24 Polyadenylation seq. is noncanonical (AATAA or AAATA); may use the polyA site adjacent to UL26;

GAGCAAT sequence4 at 1006

UL 25 1606 3279 1856 3287 or 5040 Herpesvirus UL25 Polyadenylation seq. is noncanonical (AAATAA); may use the polyA site adjacent to UL26

UL 26 3357 5006 3288 or

3305

5040 Herpesvirus assemblin, aas 30-250 of 549

TATA is noncanonical (AATAA or TTTAA)

UL26.5 4125 5006 4043, 4059, 4389,

or 4087

5040 Turtle herpesvirus UL26.5

TATA is noncanonical (TATTT or TAATAA)

Supplemental Data Table 1: Putative Transcription Signals in the FPTHV contig

UL 27

(gB)

7713 5155 7445 5261 Herpesvirus gB CCAAT sequence5 at 7608

UL 28 9962 7710 10147 7920 or 5261 Herpes processing and transport protein

Could co-utilize the polyA site adjacent to UL27

UL 29 13735 10877 13683 or

13882

10091 Herpes single-stranded DNA binding protein

TATA is noncanonical (TATTTA or AATAA); GC box6 at 13780

UL 30

(pol)

13896 17171 13686 17397 DNA polymerase B TATA is noncanonical (ATAAAA) but precedented in VZV7; CCAAT sequence2 at 12734

UL 31 18207 17284 18275 17770 Herpes UL31 TATA is noncanonical (ATTAAA); CCAAT sequence4 at 18244; GGGCGG sequence6 at 18343

UL 32 19573 18200 19891 18275 or 17770 Herpes major envelope glycoprotein

Could co-utilize the polyA site adjacent to UL31; GC

box5 at 19900

UL 33 19805 20140 19892 21415 Herpes UL33 CCAAT sequence4 at 19513; polyadenylation seq. is absent, must utilize the polyA site adjacent to UL35;

GGGCGG sequence6 at 19423

UL 34 20153 20947 19892 21415 Herpes UL34,

residues 45-220 of 264

Polyadenylation seq. is absent; must utilize the polyA site adjacent to UL35; GC box5 at 20465

UL 35 20995 21360 20943 21415   TATA is noncanonical (TTTAA)

UL 36 Beyond edge of contig

21378 Beyond edge of contig

21394 Herpes UL36  

1ORF start is reported as the first ATG codon; in cases where the first TATA homology follows the first ATG it is likely that transcription initiates from an internal ATG

2Identified by RPS-BLAST

3 “Coterminal families” of herpesvirus genes may coutilize a polyadenylation signal (Draper et al., J. Virol. 57:1023; Proudfoot&Brownlee, Nature 263:211)

4CCAAT transcription initiation co-signal (Chodesh et al., Cell 53:11)

5(A/G)(A/G)GCAAT transcription initiation co-signal (reviewed by Corden et al., Science 209:1406)

6GGGCGG transcription initiation signal (McKnight et al., Cell 37:253)

7Noncanonical transcription signals in Human Herpesvirus 3 reviewed by Cohen&Straus, Fields Virology.