Oral Spot Practical Examination (OSPE) - 2

Shigella on Deoxycholate citrate agar (DCA)

Salmonella on Salmonella-Shigella (SS) agar.

API-20 strip test

Proteus: Urease positive

CharacterKlebsiellaEnterobacterSerratia

Motility-++

Indole+--

Red pigment--+

Quellung reaction

+--

Differences between Klebsiella, Enterobacter, Serratia

Widal tube agglutination test

ConditionDoubtful Significant

Typhoid Fever 1:160 1:320

Paratyphoid Fever 1:80 1:160

Interpretation of Widal test

Colicine typing of Shigella

Swarming of Proteus

Pseudomonas on Blood agar

.

CETRIMIDE AGAR: Selective for P. aeruginosa

Positive Citrate test for Pseudomonas

Pseudomonas pyocine typing

Bacteroides fragilis: Neomycin selective medium

Bacteroides sensitive to metronidazole disc

Campylobacter Gram negative smear

Campylobacter on Butzler medium

C. jejuni hydrolyze hippurate

Gram stain of Vibrio cholerae

TCBS Agar - Vibrio

Alkaline peptone water and Selenite - F

V. Cholerae : Cholera red reaction

Haemophilus culture on chocolate agar

Haemophilus influenzae: X and V factors

Carbon dioxide candle jar

Fluorescent antibody stain.

Pernasal swabs

Bordetella on Bordet - Gengou medium

Bordetella on selective charcoal cephalexin blood agar

Slide latex agglutination test with specific antisera

Yersinia bipolar safety pin Giemsa staining

Yersinia: non-lactose fermenter on Mc Conkey

Francisella tularensis: on chocolate cysteine agar

Pasteurella multocida: non-hemolytic, mucoid colonies

Blood culture bottles

Brucella: Blood culture Castaneda diphasic bottle

Brucella on tryptone soya agar

B. abortus & meletensis grow in presence of basic fuchsin

Brucella : Standard Agglutination Test

Species

CO2

H2S (days)

Growth in presence of Urease

12345Basic fuchsin

ThioninMethyl violet

B. melitensis------++++ / -

B. abortus++++--+-++

B. suis-+++++-+-+

Identification of Brucella species

Blood wet preparation for Borrelia

Leptospira interrogans

Darkfield microscope

,

Treponema pallidum

Treponema pertenue

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Treponema carateum

VDRL TEST

Treponema Pallidum Immobilization

Treponema pallidum Hemagglutination test

VDRLRPRSpecimenOnly serum

used, serum heated

Serum & plasma used, no heating

AntigenDiluted before use

Not diluted

Reading of results

Microscopically from slide

Macroscopically from card

TechniqueDifficultEasy

Sensitivity

Less sensitiveMore sensitive

False positiveLess oftenMore often

Comparison of VDRL AND RPR

Legionella pneumophila: Gram stain

L. pneumophila on selective Mueller Hinton agar

Immunofluorescence Microscope

Branching rods of Nocardia asteroides

Gram-positive branching rods of Actinomyces

Sulpher granules of Actinomyces

Disc diffusion sensitivity test

Control Organism

Control Organism

Test Organism

E - TEST SENSITIVITY TECHNIQUE

Rosco Neo-Sensitabs Sensitivity Test

Antibiotics synergy test on Mueller–Hinton agar

Antibiotics antagonism on Mueller–Hinton agar

Chlamydia - Giemsa stain

Chlamydia Mc Coy tissue culture

Chlamydia - CFT

Mycoplasma agar: Colonies have fried egg shape

Mycoplasma : A positive cold agglutinin test

Sites of inoculation of fertile eggs

Coxiella: Culture in yolk sac of chick embryo

Giemsa stain: Rickettsia inside the cell

:

Rickettsia: Weil-Felix Test

Helicobacter pylori: Spiral gram negative rods

Helicobacter pylori on selective Butzler medium

Water Examination: The multiple tube technique

Water Examination: Viable plate countiable plate count

Milk Examination: Methlylene blue reduction test

Milk Examination: Milk ring test:

Milk Examination: Phosphatase testPhosphatase test::