73

ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

  • Upload
    others

  • View
    1

  • Download
    0

Embed Size (px)

Citation preview

Page 1: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor
Page 2: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor
Page 3: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

ORAL PRESENTATION

Page 4: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES

Dr.Vijaya Chandra Reddy Konda,* Associate Professor of Pharmacology, SVIMS Dr.V.Vanajakshamma, Professor of Cardiology, SVIMS ABSTRACT Cardiovascular diseases (CVD) are the leading cause of disease burden and deaths globally. In spite of much advancement in treatment, annually much of the financial resources are spent on care of patients with CVD, and this is expected to increase 2- to 3-fold over the next several decades. There is a need for novel and inexpensive interventions that will be of benefit to patients and can contribute to the primary and secondary prevention of CVD. The concept of “Meditation” is currently gaining popularity worldwide owing to its various health benefits and other advantages such as safety and ease of practice. There is also considerable evidence accumulating related to benefits on health, especially cardiovascular health. Stress plays a major role in coronary artery disease (CAD). Most published studies report some improvements in levels of perceived stress, mood, anxiety, depression, quality of sleep, or overall wellbeing in meditation group. Studies of meditation on lower of blood pressure have shown wide variation in magnitude of reduction of systolic blood pressure. Few studies of meditation on atherosclerosis progression demonstrated the regression of carotid intimal medial thickness in participants of mediation group. Studies on effect of meditation on endothelial function, primary and secondary prevention of CAD has shown benefit but yet to be proved in larger studies. In view of possible benefits of meditation in cardiovascular risk reduction and considering low costs and low risks of meditation, American Heart Association stated that meditation may be considered as an adjunct to guideline-directed cardiovascular risk reduction.

Page 5: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

ARSENIC EXPOSURE ALTERED THE GUT MICROBIOME AND NOD2 OF ILEUM THROUGH IMMUNE DISRUPTION AND REGULATED

INFLAMMATION IN MICE: CORRELATION WITH COLON CANCER MARKERS

Chiranjeevi Tikkaa, Ram Kumar Mantharia, Ruiyan Niua, Zilong Suna, Jianhai Zhanga, Jinming

Wanga, Bhaskar Matchab, Jundong Wanga*

a Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu-030801, Shanxi, China.

b Department of Zoology, SVU College of Science, Sri Venkateswara University, Tirupati-517502, Andhra Pradesh, India. *Corresponding author: Prof. Jundong Wang, Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu-030801, Shanxi, China; Mobile No: +86 13603546490; Tel.: +86-354-6288206; Fax: +86- 354- 6222942 E-mail: [email protected] ABSTRACT This study was designed to determine whether the altered gut microbiome and Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) regulates colon cancer markers via immunological alterations and inflammation initiator genes in arsenic (As) induced mice. Mice were exposed to different As concentrations (0, 0.15, 1.5, and 15 mg/L As2O3) for two different exposure periods (3 and 6 months) to determine the functional effects of As in the gut microbiota by 16S rRNA gene sequencing and then to evaluate the immunological alterations of immune cells NOD2, colon cancer markers, inflammatory and anti-inflammatory cytokines. Based on our results, we found that NOD2 is an essential regulator which controls the commensal bacterial flora. Interestingly, both gut microbiome and NOD2 were dysregulated by the prolonged As exposure. Altered microbiome disrupts immune function due to the dysfunction of NOD2, however, imbalanced immune system leads to secrete inflammatory cytokines and initiate inflammation by suppressing the anti- inflammatory cytokines. Inflammation initiator genes suppress the adenomatous polyposis coli protein (APC) which regulates the β-catenin. This study provides the mechanism on how As alters immune system and promotes the colon cancer and new mechanism for As to dysregulate NOD2 leading to APC mediated colon cancer. KEY WORDS: Arsenic, Ileum, Microbiome, NOD2, colon cancer.

Page 6: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

LIPIDIC AND PHOSPHOLIPIDIC PRODRUGS: AN APPROACH FOR IMPROVING DRUG DELIVERY AND DRUG THERAPY

P.SYAMALA, SVU COLLEGE OF PHARMACEUTICAL SCIENCE SV

UNIVERSTY References: Stella, v.j prodrug as therapeutics. Expert opin. Ther. Patents2004.14,277-280 ABSTRACT Prodrugs are bioreversible derivatives of active drug moieties and are designed to undergo conversion in the body, thereby releasing the active parent drug, thus can overcome certain physiological barriers to accomplish its desired pharmacological effect. Hydrophilic prodrug and Lipidicprodrug are two different approaches of prodrug, lipidicprodrugs or drug–lipid conjugates, have the drug covalently bound to a lipid moiety, improves oral drug delivery System and drug therapy as it takes the benefit of physiological lipid metabolic pathways. Phospholipid-based (PL) prodrug, where the lipid carrier being a phospholipid, have increased lipophilicity comparing to the parent drug, thus improving the pharmacokinetic profile and bioavailability of the parent drug and accomplish drug targeting. It is an exciting gateway for treating inflammatory diseases and cancer diseases providing an elegant solution for insufficient drug retention and targeting. PL conjugates can be optimized by computational modeling techniques like molecular dynamic simulations that are established for the elucidation of macromolecular structure-to-functional relationship, making the development process more effective and less empirical.Modern computational methodology, based on thermodynamic integration and histogram analysis, is an important tool for increasing specifictargeting of PL-based prodrug, which shows great potential in improving different drug delivery aspects. The present study aims to review novel computational modeling techniques that are employed for the predictions of PL-prodrug activation, to achievemore accurate and biologically relevant results. Keywords: prodrug, lipid prodrug, phospholipid prodrug, drug delivery, drug therapy, modern computational methodology.

Page 7: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

ACETATE KINASE A POTENTIAL DRUG TARGET IN THE DEVELOPMENT OF ANTIMICROBIALS AGAINST

STAPHYLOCOCCUS AUREUS INFECTIONS S. Subbarayudu and P.V.G.K Sarma

Microbial Genetics Laboratory, Department of Biotechnology, Sri Venkateswara Institute of Medical Sciences, Tirupati. ABSTRACT Staphylococcus aureus is a facultative anaerobic pathogen causing invasive opportunistic infections and spreads infection through biofilm formation. Staphylococcus aureus expresses many virulence factors and utilizes the accumulated acetate as a carbon source via the Pta-Ack pathway (phosphotransacetylase, Pta, and acetate kinase, ackA). In which ackA catalyzes the substrate-level phosphorylation during acetate metabolism is an important secondary energy-yielding pathway, since, under carbon overflow conditions, TCA cycle activity is limited by carbon catabolite repression; which favors biofilm formation. This implies a crucial role of ackA in the growth and pathogenesis of S. aureus. In this current study, we have observed that elevated ackA activity and biofilm formation in multidrug-resistant strains of S. aureus grown under anaerobic conditions. S. aureus ackA gene was cloned, sequenced and functionally expressed in Escherichia coli DH5α and recombinant ackA (KC954623.1) shows a molecular weight of 44 kDa. The structure of S. aureus ackA built from the deduced amino acid sequence was superimposed with Mycobacterium avium (3P4I), Salmonella typhimurium (3SLC) to find out the structural identity using SWISS-MODEL and PyMOL gave an RMSD value 1.877Å, 2.141Å indicating very high homology and like in S. aureus in these organisms also acetate is used as carbon source. The elevated expression of active ackA in anaerobic conditions wherein biofilm formation is also high and hence ackA plays an important role in the pathogenesis of S. aureus. This acetate kinase no counterpart in the human system could be a useful drug target in the development of antimicrobials against S. aureus.

Page 8: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor
Page 9: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

BIOTECHNOLOGY ENTREPRENEURSHIP: CHALLENGES AND OPPORTUNITIES

Dr. D. Sambashiva1 and Saranya Y2 1. Department of Biotechnology, Nizam College, Osmania University,

Hyderabad, Telangana- [email protected] 2. Department of Chemistry, University College of science, Osmania

University, Hyderabad, Telangana- [email protected] ABSTRACT

Nature, Science and Technology came into the focus in today’s world with an opportunity to play a major role in varied disciplines. Biotechnology is a far- reachingsector which comprises of a bundle of technologies that operates the natural idle things into modified outcomes with its increased potential and sustainable return of products for beneficial of human mankind. The goals of Biotechnology are endless, it is being employed for the benefit of humans and environment with corresponding development in scientific research and technological development. Biotech ecosystem that can both create and develop new low-cost products and bring them to market.The methods and techniques in biotechnology are being practiced in various fields such as Food, Chemical, Industrial, Medical, Marine, Pharmaceutical, Nano-science, biomedical, etc. The Indian Biotech market is raising across the globe in the field of Human health, Pharma and Agro Products but failing in the development of Entrepreneurial activity at its roots, in graduate level due to lack of facilities, skill development and training to become an entrepreneur.Biotechnology is still in its infancy to find the answers of nature, understand the power beyond science.Biotechnology has its own advantage and disadvantage. The Government of India Creating awareness among the Younger generations to become a sustainable entrepreneur by various programmes. Biotechnology creates a path to enter into the world trade with technology innovations and inventions.Establishment of other national and international institutes to support business among nations of the world byGovernment organizations for social and cultural development.Biotechnology has the ability to look at the environment, identify opportunities to improve the environmental resources and implement action to maximize those opportunities for sustainable growth of the nation.

Page 10: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

MILK EXOSOMES: ANTIVIRAL PROPERTYAGAINST DENGUE VIRUS

Vengala Rao Yenuganti1, Sumbul Afroz2,Bharadwaj Chandrima2,Madhuri Subbiah3Reddanna Pallu1, Nooruddin Khan2 Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana, India, 500046. Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana, India, 500046. ABSTRACT

Exosomes are nano-sized extracellular vesiclessecreted by most of the cells in mammals and isfound in different body fluids including milk. These are involved in communication andcarry miRNA, mRNA and DNA.In some parts of India, people believethat goat milk cancure dengue infection. However, there is no proper scientific backup. This curing effect of goat milk is possibly due to the presence of extracellular vesicles especially exosomes. To test this, in our present study we are tryingto test antiviral property of exosomes on Dengue virus (DENV). Exosomes from the goat milk were isolated by differential centrifugation process. The physical properties of milk exosomes was characterized by Transmission Electron Microscope (TEM) and Dynamic Light Scattering (DLS),and molecular characterization of exosomes was done using Real Time PCR and western blot.Therapeutic potential of milk exosomes on dengue infection was examined in- vitro. Results showed the presence of 30-150 nm size extracellular vesicles,milk exosomes miRNA markers(mir-26a, miR-148a, mir-30d, miR-30a-5p and miR-423- 5p)and exosomal protein markers CD-63, respectively.Pre and post treatment of exosomes effectively inhibits infection of DENV. In addition to this goat milk exosomes inhibited the replication of DENV. Heat inactivation of exosomes leads to the loss of its anti-viral effect. In summary,milk derived exosomes hastherapeutic potential to combat dengue infection. Key words: Exosomes, virus, miRNA, DENV and Goat Email: [email protected]

Page 11: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

REGULATION OF SURVIVAL, ANGIOGENESIS, INFLAMMATION AND CARDIOMYOCYTES DIFFERENTIATION BY DELTA OPIOID

SIGNALING IN HUMAN MESENCHYMAL STEM CELLS

VINOD KUMAR, VIT, VELLORE ABSTRACT Myocardial infraction (MI) is a leading cause of heart failure and death in the modern world. Current treatments an extend the patient life but they are unable to regenerate the damaged myocardial tissue. Therefore, alternative ways need to find to regenerate the damaged myocardium. Stem cells therapies are better approaches for tissue regeneration. Even though stem cells have enormous regenerative potential, after transplantation to ischemic site their outcome is very poor because, 1. Nutritional deprivation, 2. Lack of angiogenesis and3. Inflammation-rich zones and 4. Lower number of stem cells (MSCs) differentiate into cardiomyocytes (CMs), which prevents the reparativeprocess. Therefore, in our study for the first time we demonstrated the role of δ-opioid receptor (DOR) signaling and their therapeutic potential on hMSCs. The activation of DOR signaling has shown increased cell survival and ant- inflammatory effect in serum deprived culture conditions via DOR/PI3K/AKT pathway. Also,DOR activation boost hMSCs to cardiomyocytes differentiation and reduced the pro-inflammatory cytokine secretion and increased angiogenesis, along with small molecules treatment (zebularine, Oxytocin and activin A). These results demonstrate that the activation of DOR signaling on hMSCs could have therapeutic potential for MI.

Page 12: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

REGULATORY REQUIREMENTS OF MEDICAL DEVICES IN INDIA LAVANYA .V

Research scholar from SRM college of Pharmacy , SRM institute of science and technology, chennai. Abstract India represents the compelling market opportunity for global medical device manufacturers, with the majority of medical devices sold in India were imported from other countries. Substantial growth of the Indian medical device industry is expected to be driven by the current population spending rate for medical devices.Today millions of patients depends on medical device based treatment for the management and diagnose of several diseases. Quality and safety of device is depends upon the regulatory guidelines. Medical device manufacturing in India should be taken seriously due to large population and the potential severity of the consequences of introducing inferior and unsafe products to the market-place. Guidelines and regulations are required for monitoring the entry of such devices into the use of public health. The regulations define requirements of medical device design, development and manufacture to ensure that products reaching market which are safe and effective. Presently in India CDSCO is the governing regulatory body for the regulation of Medical devices. Which are with time, amendment introducing in the law will provide safety and assurance to public health.

Page 13: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

RESEALED ERYTHROCYTES: NEW TREND IN TREATMENT OF ASTHMA

Vasanth PM, A.Neelima, Deekshitha SR, Kavya P

S.V.U.College of Pharmaceutical Sciences, Sri Venkateshwara University, Tirupati - 517501. A.P. Carrier erythrocytes are emerging as one of the most promising biological drug delivery systems investigated in recent decades. Beside its biocompatibility, biodegradability and ability to circulate throughout the body, it has the ability to perform extended release system of the drug for a long period. The ultimate goal of this study is to introduce a new carrier system for Salbutamol, maintaining suitable blood levels for a long time, as atrial to resolve the problems of nocturnal asthma medication Therefore in this work we study the effect of time, temperature as well as concentration on the loading of salbutamol in human erythrocytes to be used as systemic sustained release delivery system for this drug. After the loading process is performed the carrier erythrocytes were physically and cellulary characterized. Also, the in vitro release of salbutamol from carrier erythrocytes was studied over time interval. From the results it was found that, human erythrocytes have been successfully loaded with salbutamol using endocytosis method either at 25 Co or at 37 Co. The highest loaded amount was 3.5 mg/ml and 6.5 mg/ml respectively. Moreover, the percent of cells recovery is 90.7± 1.64%. Hematological parameters and osmotic fragility behavior of salbutamol loaded erythrocytes were similar that of native erythrocytes. Scanning electron microscopy demonstrated that the salbutamol loaded cells has moderate change in the morphology. Salbutamol releasing from carrier cell was 43% after 36 hours in phosphate buffer saline. The releasing pattern of the drug from loaded erythrocytes showed initial burst release in the first hour followed by a very slow release, obeying zero order kinetics. It concluded that salbutamol is successfully entrapped into erythrocytes with acceptable loading parameters and moderate morphological changes, this suggesting that erythrocytes can be used as prolonged release carrier for salbutamol. Keywords: Carrier erythrocytes, asthma, endocytosis, salbutamol, osmotic fragility.

Page 14: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

NOVEL DEVELOPMENT OF LYMPHATIC DRUG DELIVERY IN AUTOIMMUNE DISORDERS EXHIBITING DIABETES MELLITUS

KAVYA PAPPU, SVU COLLEGE OF PHARMACEUTICAL SCIENCES, SV UNIVERSITY

ABSTRACT Nearly 23.6 million people are affecting diabetes in children and adults, these may replenish their work productivity and premature death. This leads to an effect on heart disease, renal disorders, eye problems, and some severe conditions leads to death survey of the recent decade in the range of 4.4% less for woman and 7.1% less for men. High production of glucose leads to a condition called diabetes mellitus and causes damage in the large and small blood vessel in long term diseased state. A long-term medication can be overcome in relates to relapse rates and prevalence rates few instances in proximal medication mainly autoimmune disorders can exhibit hypertension and asthma. Glucose levels significantly increase in thoracic duct lymph flow and transport of dextrin into lymph nodes which decrease the insulin requirement and it restored normal glycaemic condition. The lymphatic spores are predominantly converted to large holes at lymph vessels and the permeability enhanced at lymph nodes by reducing the antigen transportation. The cervical lymph node is more prevalent in the metformin group than in the non-Metformin group. Pancreatic beta cells are destroyed by type-1 diabetes and carbamazepine ponesimod inhibits the spreading of T cells responses to specific glucose 6 phosphate catalytic subunit related to protein and deficit the inhibition in the differentiation of autoreactive T cells. Pancreatic lymph nodes play a key role in the formation of autoreactive T cells and in the pathogeneses of Type -1 diabetes. Developing targeted delivery of drugs to the lymph nodes represents a significant milestone and holds the potential to increase the efficacy of immune therapies. Keywords: Lymph nodes, hypertension, autoimmune diabetes, beta cells

Page 15: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

ANTIMICROBIAL ACTIVITY OF STRYCHNOS NUX-VOMICA LEAF AND

ROOT EXTRACTS AGAINST HUMAN PATHOGENS Murali Krishna Thupurani*a Rajender vadlluri a, Epur Manoj Kumar Reddy b,

Sakinala Jeevanchandrab

aDepartment of Biotechnlogy Chaitanya Institute of Science and Technology, CIST (Deemed to be University), Warangal-506001 Telangana,India.

bDepartment of Microbiology, Chaitanya Institute of Science and Technology, CIST (Deemed to be University), Warangal-506001 Telangana,India *. Corresponding author email:

[email protected] Ph.: 9848835350

ABSTRACT Background: Antibiotic resistance and biohazard nature of synthetic drugs is contemporary threatening issues in the heath sector. The alternative research is focused on plants which attribute for various compounds that exhibit therapeutic properties. Generation of free radicals can cause oxidative stress which play key role in cause of different diseases such as cancer, diabetes, cardiovascular diseases, Parkinson’s disease, Alzheimer’s disease etc,. There are only few reports were published on antimicrobial and antioxidant activities of S. nux-vomica. Hence, the aim of this study was to evaluate the antimicrobial and antioxidant efficacy against tested human pathogenic organisms and free radicals respectively. Methods: The extraction procedure was carried out in soxhlet apparatus using low polar to high polar solvents. Agar well diffusion method and broth dilution method was carried out to evaluate antibacterial and antifungal assay of S. nux-vomica leaf and root extracts on selected bacterial and fungal strains. The efficacy of antioxidant activity of extracts was evaluated using DPPH, Nitric oxide, Superoxide, Hydrogen peroxide, Hydroxyl radical ions. Results: Among the extract tested, the ethyl acetate fraction of root was found active against tested bacterial and fungal strains. E. coli with 17.6 and 25.8 mm Zone of inhibition was found more susceptible at 75 and 100µg/ml respectively. The free radical scavenging activity of leaf and root extract is also found to be significant against all tested free radicals. The ethyl acetate fraction of root was found more active against DPPH by producing highest antioxidant percentage 80.9% at 250µg/mL. Conclusion: The extracts of S. nux-vomica showed antimicrobial and antioxidant activities against tested human pathogens and as well on tested free radicals. The activity was found in concentration depended manner. Further studies are required to isolate the actual principle that responsible for activity. Key words: S. nux-vomica, antimicrobial, antioxidant, ethyl acetate

Page 16: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

POSTER PRESENTATION

Page 17: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P1

GREEN SYNTHESIS AND SPECTRAL CHARACTERIZATION OF SILVER NANOPARTICLES FROM MUSA PARADISIACA AND EVALUATION OF ITS PHYTOCHEMICAL, ANTI-OXIDANT,ANTI-BACTERIAL AND ANTI- PROLIFERATIVE ROLE IN OVARIAN CANCER Venkata Satya Harika G and Dr P Josthna*

Sri Padmavathi Mahila Visvavidyalayam (Women’s University), Tirupati- 517502 Nanotechnology is predicted as an innovative change in various fields of medicine. Nanoparticles are used universally to acquire hygiene, non-toxic, Eco-friendly technology. Biosynthesis of Silver Nanoparticles (AgNPs) have extensive range of applications. Biosynthesis of nanoparticles using plants and microbes reduce toxicity and enhance biocompatibility of the particles which are produced. Green synthesis makes use of the principle of metal ions reduction in aqueous extracts of plant parts that may lead to development of nanoparticles. AgNP with plant extract with micro scale and nano scale porosity can help increase drug loading efficacy and boost up the immune response and show the adjuvant activity to destroy malignant neoplasm in the treatment of cancer. Musa paradisiaca pseudo stem extract was synthesized by AgNPs. The UV-Vis spectra gave an SPR band at 423 nm for 0.2mM for 30min.The average diameter of the AgNP was found to be 119nm. The anti-bacterial property of AgNPs was known from ancient times and silver nanoparticle is used as bactericidal agent. Furthermore, the antibacterial activity of synthesized AgNPs against gram- negative and gram-positive micro-organisms. An enhanced antimicrobial activity was observed with AgNPs synthesized from pseudo stem extract. The resulting AgNPs was isolated and characterized with various other instrumental methods such as FTIR, XRD, TEM.Anti-Oxidants are protecting substances to the cells from destructive effects. So they are regarded as protective anti-carcinogens. Invitro anti-proliferative activity was performed and IC 50 values are noted. The biosynthesized AgNPs were proved to be very effective scavengers. Keywords: Nanotechnology, Silver Nanoparticles, Biosynthesis, Musa paradisiaca Pseudo Stem extract, Antimicrobial activity, anti -oxidants, FTIR,TEM,MTT,Flow cytometry.

Page 18: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P2

FORMULATION OF ZINC OXIDE NANOPARTICLES IN NANO

SANITIZER Devasena.G1, M. Rajaswi Devi2, Prof.P. Suvarnalatha Devi3 1,2Department of

Biochemistry, 3Department of Applied Microbiology Sri Padmavati MahilaVisvavidyalayam, Tirupati-517502

Correspondence to Author: Devasena.G, Department of Biochemistry, Sri Padmavati MahilaVisvavidyalayam, Tirupati- 517502 Email ID:[email protected] ABSTRACT Among the different metal oxide nanoparticles, Zinc oxide nanoparticles (ZnO NP) have their own importance due to their vast area of applications such as biosensors, cosmetics, solar cells, drug-delivery, disinfectant etc. ZnO is currently listed as a “generally recognized as safe (GRAS)” material by the Food and Drug Administration,hence it is safe even if used as a food additive. The anti-bacterial mechanism of ZnO NPs involves the direct interaction between ZnO nanoparticles and the cell surfaces affecting the cell membrane permeability; afterwards these nanoparticles enter and induce oxidative stress in the bacterial cells, which results in the inhibition of cell growth and eventually cell death. Hence it can be applied as a potent hand sanitizing agent in hospitals or laboratories, as they are

and Aspergillus niger. ZnO nano hand sanitizer hence helps in preventing several nosocomial infections within 60 seconds. The NPs of ZnO showed both toxicity on pathogenic bacteria and beneficial effects on microbes, as Pseudomonas putida, which has bioremediation potential and is a strong root colonizer. The Zinc oxide Nano sanitizer perfectly ensures clean hands and forms a protective anti-bacterial layer on the skin due to its anti-microbial activity. Zinc oxide nanoparticles eliminate all known microbes, building immunity against airborne germs, bacteria and infectious viruses. Key words: Sanitizing agent, ZnO NPs, anti-microbial activity.

more effective against micro-organisms such as Bacillus subtilis, Bacillus megaterium, Staphylococcus aureus, Sarcina lutea, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Pseudomonas vulgaris, Candida albicans,

Page 19: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P3 Abstract

Skill development in biopharma Name:- R.aswani, college:- svucps,

E-mail:[email protected]

Skill intensive industries,such as manufacturing & services,are expected to

contribute more than 90%of India’s GDP by 2030. Hence skill development has

been taken as a priority project by the present government,led by our worthy

prime minister,Mr.Narendra Modi.Whilst countries such as Brazil, China,

Germany, Switzerland, UK, Canada, US, Japan & south korea boast of between

50-97% of their work force being skilled, India’s skilled workforce is under

5%.This dismal situation is driven by factors like outdated academic

curricula,shortage of quality& faculty,high student-teacher ratio,lack of

institutional industry linkages &lack of autonomy to introduce new & innovative

courses

Page 20: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P4

ANTIPROLIFERATIVE EFFECTS OF QUERCUS INFECTORIA ETHANOLIC LEAF EXTRACT AND ITS MECHANISM OF CELL DEATH ON ORAL CANCER (KB) CELLS K.R.Padma,K. Jayasree, B. Mamatha yadav and D.sireesha Department of Biotechnology, Sri Padmavati Mahila VisvaVidyalayam (Women’s University), Tirupati, Andhra Pradesh, India. Abstract The cancer cells are mostly widely resistant to various chemotherapeutic drugs which were developed in early times. Hence usage of natural products especially in possession of medicinal properties which are readily and easily accessible from plant sources for significant remedy for cancer. Among the natural resource Quercus infectoria is one of the supreme plants which contain several bioactive compounds and also study the pharmacological effect of this plant belonging to the family of Fagaceae which has shown to possess valuable source of anticancer property. Nevertheless, the scientific reports to support its In-vitro antiproliferative and apoptotic inducing activities are very rare up to date and no work was carried on In- vitro analysis using the crude extract of Q. infectoria with 70% ethanol solvent. Hence, the objective of the current research is to evaluate the phytochemicals present in Q. infectoria extract and further using relevant extract carried on for In-vitro assay for testing the cytotoxicity as well as cell viability. While ethanol extract of different concentration ranging from 25 to 400µg/ml when subjected to MTT assay, distinctively revealed the cell proliferation was suppressed by induction of apoptosis which was clearly visible after incubation for 72 hrs with concentration of (76.82 ± 4.74 µg/ml). All findings of ethanol extracts with different concentration with three independent triplicates revealed statistically significant with p˂0.01. Key words: Q.infectoria, apoptosis, MTT assay, bioactive compounds

Page 21: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P5

SPERM CHARACTERISTICS IN F1 OFFSPRING OF WISTAR RATS MATERNALLY EXPOSED TO ARTEMISIA ANNUA L. EXTRACT

(AAL) DURING GESTATION Y. Mrunalini1, P. Sreenivasula Reddy2 and R. Usha1

1Department of Biotechnology, SPMVV, Tirupati 517502, India 2Department of Zoology, Sri Venkateswara University, Tirupati 517502, India Abstract

Artemisinin extracted from Artemisia annua L. is commonly used in malaria therapy. Malaria in pregnancy is a major public health issue and infected pregnant women need prompt treatment with effective drugs. Many antimalarial drugs have been associated with reproductive dysfunction. Artemisinin is an antimalarial drug previously reported to induce neurotoxicity and embryotoxicity in animal models. The indiscriminate use, abuse and patients noncompliance to normal prescription of artemisinin and its derivatives are a common practice during the treatment for drug-resistant malaria parasites in most developing countries. This study investigated the influence of Artemisia annua extract on the testicular and epididymal sperm characteristics. Pregnant Wistar rats were given 100, 200, or 400 mg/kg bw Artemisia annua extract (AAL) daily from gestation day (GD) 7 to 9. At parturition, newborns were observed for clinical signs and survival. Male pups from control and AAL exposed animals were weaned and maintained up to postnatal day (PD) 90. Body weights were decreased and the tissue indices were comparable on PD 90 in male rats exposed to AAL during embryonic development when compared with controls. Significant reduction in the testicular daily sperm production, epididymal sperm count, number of viable, motile, and hypo-osmotic tail coiled sperm was observed in experimental rats. These results indicate that in utero exposure to AAL compromised reduction in spermatogenesis which may leads to reproductive deficits in individuals at adulthood.

KEY WORDS: Artemisinin; Gestation; Sperm; Spermatogenesis;

Page 22: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P6 PREPARATION AND OPTIMIZATION OF JATROPHA GOSSYPIFOLIA

LEAF EXTRACT LOADED SCAFFOLDS

A.Neelima S.V.U.College of Pharmaceutical Sciences, Sri Venkateshwara University, Tirupati - 517501. A.P. ABSTRACT The aim of present study was preparation and optimization of Jatropha gossypifolia leaves extract loaded scaffolds for wound healing activity. Scaffolds are termporary dressings of wounds. These are medicated adhesives placed on skin to deliver specific dose of medication through skin. When used in wound healing, these promote healing at injured area of the body. Recently biological dressings became very popular because of their properties like quicker wound healing and retarding wound infections. Jatropha gossypifolia L. (Euphorbiaceae) is a medicinal plant largely used in folk medicine and used for the treatment of some diseases. Metanolic Maceration extract of Jatropha gossypifolia leaves is used to treat skin diseases which include infective process like carbuncle, furuncle, abscesses, and other conditions like eczemas, rashes including chicken -pox, measles. Jatropha gossypifolia working mechanism of action possessing the antimicrobal and antioxidant activity. In this study scaffolds are prepared by using polymers like chitosan, sodium alginate. Extract loaded scaffolds extract is loaded. Physical parameters were evaluated like Thickness (48.6 ±1.446 (54.6 ± 1.055), folding endurance (303 ± 3.5), swelling index (275%), Tensile parameters(0.114), Brust Strength(0.0057). FT-IR spectra of different polymers and selected best scaffolds were studied making a KBr disc. The SEM micrographs of CSA7 and CSA11 scaffolds showed smooth, flimogenic continuous, compact and homogeneous, more uniform surface indicated complete miscibility between polymers. The percentage of wound contraction for untreated and treated groups was measured on 7th, 14th, 21th, 28th post wounding day and the results. the group IV shows the complete healing on 14th day of the extract loaded scaffolds. and the group V shows the complete healing on the 21th day in the treated with marketed formulation. where as in all parameters like anti microbial activity, histopathological studies, photographs, tensile strength of treated and normal skin are concluded. Keywords: Jatropha gossypifolia, leaf extract, scaffolds.

Page 23: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P7

PHYTOCHEMICAL SCREENING OF ETHANOLIC AND

AQUEOUS EXTRACT OF LANTANA CAMARA LEAVES K.Muni raja lakshmi.

S.V.U College of Pharmaceutical Sciences, S.V.University, Tirupati,(A.P)-517502. ABSTRACT Herbal medicinal plants have played a critical role in maintaining human health and quality of human life. Many of existing medicinal systems such as Ayurveda, Unani, Homoeopathy, Naturopathy, Siddha have been utilizing plants as an effective medicine to cure many harmful diseases. In the present research, Lantana Camara which is an important medicinal plant of the family Verbenaceae with several medicinal uses in traditional medication system has been used to cure many aliments. Leaves are used to treat catarrhal infections, tetanus, rheumatism, malaria, asthma, ulcer, swelling, eczema, tumour, high blood pressure, bilious fever, ataxy of abdominal viscera, sores, measles, fevers, colds and high blood pressure. Lantana Camara is reported to possess essential oils, phenolic compounds, flavonoids, carbohydrates, proteins, alkaloids, glycosides, cardiac glycosides, phenylethanoid, oligosaccharides, quinine, saponins, steroids, triterpenes, sesquiterpenoids and tannin as a major phytochemical group. This plant is reported for various medicinal properties like anti-bacterial, anti-fungal, anti-inflammatory, anti-diabetic, wound healing, anti-oxidant, anti fertility, anti-motility, hepatoprotective, cytotoxic, larvicidal, anti- urolithiasis activities. Lantana Camara was shade dried macerated with ethanol and aqueous based on their polarity following cold extraction method and filtrates were concentrated by rotary evaporator to yield was about 4% dense residues. Based on the phytochemical analysis and 99% ethanol and aqueous extract were selected. Key words: Lantana Camara leaves, ethanol, aqueous extract, phytochemical constituents.

Page 24: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P8

INHIBITOR DESIGN STRATEGY FOR INTERSTITIAL COLLAGENASE MEDIATED MALIGNANCIES

Katari Sudheer Kumar*, Sharon Priya Alexander and Amineni Umamaheswari** Bioinformatics Centre, Department of Bioinformatics, SVIMS University, Tirupati- 517507 *Presenting author **Corresponding author: [email protected] Abstract:

In cancers, interstitial collagenase devastatingly degrades triple helical forms of collagen leads to extra cellular matrix destruction in breast, prostate, brain, lung and colorectal cancers enabling cells to gain tumorigenicity properties like epithelial to mesenchymal transition (EMT), invasion, angiogenesis and metastasis. Among the eleven matrix metalloproteinase 1 (MMP-1) crystal structures in the protein data bank, four structures have diverse co-crystal ligands (1CGL-0ED, 1HFC-PLH, 966C- RS2 and 2TCL-R04) were selected for structure based rational drug design. Four e- pharmacophores (AAADR, AADD, ADR, AADDDD) were generated and screened against in-house library of 28.1 million compounds using Phase shape’s efficient algorithm. Two compounds have better binding affinities in multiple docking strategies (rigid receptor docking and quantum polarized ligand docking) and binding free energies compared to the four co-crystal ligands. Lead1 and R04 have QPLD XP GScore of -10.16 kcal/mol and -9.367 kcal/mol; ΔGScore of -89.123 kcal/mol and - 68.682 kcal/mol respectively. Further the two leads were validated alone and also with four co-crystal ligands by receiver operative characteristic curve metrics (ROC: 1.0 and ROC: 0.96) in the presence of thousand decoy molecules obtained from directory of useful decoys dataset. The stability of best lead MMP-1 complex surpasses the MMP-1-R04 complex in natural physiological conditions were analyzed by 300 ns molecular dynamics simulations (MDS). Lead1 formed more interactions (3,395) with catalytic residues His218, Glu219, His222, His228 and Zn lesser deviations, lesser fluctuations and lesser energies than the R04 with MMP-1 in 300 ns MDS. Hence, proposed leads cease MMP-1 protease activity on interstitium which in turn halts various cancer hallmarks. Keywords: Cancers, interstitial collagenase, e-pharmacophores, docking, ROC metrics and MD simulations.

Page 25: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P9

TRANSPLATATION OF MITOCHONDRIA FOR THERAPEUTIC USE

IN TRANSLATIONAL MEDICINE A.ROHINI, J.MAHESWARI, N.V.MRUNALINI, R.LAILA

Department of Biotechnology, Sri padmavathi Mahila VisvaVidyalayam (women’s University), Tirupathi, Andhra Pradesh, India.

Abstract Mitochondria play a key role in the homeostasis of the vast majority the body’s cell. In the myocardium where mitochondria constitute 30% of total myocardial cell volume, temporary attenuation or obstruction of blood flow and as a result oxygen delivery to myocardial cells (ischemia) severely alters mitochondrial structure and function. These alterations in mitochondrial structure and function occur during ischemia and continue after blood flow and oxygen delivery to the myocardium is restored, and significantly decrease myocardial contractile function and myocardial cell survival. We hypothesized that the augmentation or replacement of mitochondria damaged by ischemia would provide a mechanism to enhance cellular function and cellular rescue following the restoration of blood flow. Keywords: Ischemia, homeostasis, attenuation, restoration,

Page 26: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P10

ANTIPROLIFERATIVE ACTIVITY OF FLEMINGIA WIGHTIANA ETHANOLIC LEAF EXTRACT IN CARCINOGEN-INDUCED RODENT

COLORECTAL CANCER M. Lakshmi Priya1,C. Varadarajulu Naidu2and Josthna Penchalaneni1*

1Department of Biotechnology, Sri Padmavathi Mahila University, Tirupati 517 502. Andhra Pradesh, India 2Department of Biotechnology, Dravidian University, Kuppam, 517426, Andhra Pradesh, India ABSTRACT There are currently distinct approaches for treatment of cancer in which chemotherapy can be effective, but a huge percentage of healthy cells with cancer cells will be lost in this strategy owing to drug non-selectivity. Therefore, death of tumour cells in the presence of normal cells without destroying natural cells is the most important issue in cancer treatment. At this juncture, anticancer drugs from natural resources such as crops are one solution, therefore it is crucial to monitor cytotoxic compounds and screen raw plant extracts. The current research was therefore intended to determine the bioactive compounds in Flemingia wightiana's whole leaf ethanolic extract. For the phytochemical screening of ethanolic extract, gas chromatography-mass spectrometry (GC-MS) was performed. Further proceeds to examine the role of Flemingia wightianaethanolic extract (FWEE) in regulating apoptosis by DMH induced carcinogenesis model. FWEE significantly promoted the cell death through apoptosis mediated by caspase-3 activation and increased pro-apoptotic Bax protein and decreased levels of anti-apoptotic proteins (Bcl-2, PCNA) which were supported by histological observations in colon and Oxidative stress analysis by estimating different oxidative enzymes. The results of this study have been found to be significant and have established FWEE's possible effectiveness against colon carcinogenesis. Keywords: Flemingia wightiana, GC-MS , HCT 116 cell lines , MTT assay, Invivo , 1, 2-Dimethyl Hydrazine, Apoptotic and Anti- apoptotic proteins.

Page 27: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P11

GREEN SYNTHESIS OF LEAF ASSISTED ZINC OXIDE NANOPARTICLES AS HAND SANITIZER

Devasena.G, M. Rajaswi Devi, Prof.P. Suvarnalatha Devi Department of Biochemistry, Sri Padmavati MahilaVisvavidyalayam, Tirupati-517502 Correspondence to Author:Devasena.G Email ID:[email protected] ABSTRACT Among the different metal oxide nanoparticles, Zinc oxide nanoparticles (ZnO NP) have their own importance due to their vast area of applications such as biosensors, cosmetics, solar cells, drug-delivery, disinfectant etc. The anti-bacterial mechanism of ZnO NPs involves the direct interaction between ZnO nanoparticles and the cell surfaces affecting the cell membrane permeability; afterwards these nanoparticles enter and induce oxidative stress in the bacterial cells, which results in the inhibition of cell growth and eventually cell death. Hence it can be applied as a potent hand sanitizing agent in hospitals or laboratories, as they are more effective against micro-organisms such as Bacillus subtilis, Bacillus megaterium, Staphylococcus aureus, Sarcina lutea, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Pseudomonas vulgaris, Candida albicans, and Aspergillus niger.ZnO nano hand sanitizer hence helps in preventing several nosocomial infections within 60 seconds. The NPs of ZnO showed both toxicity on pathogenic bacteria and beneficial effects on microbes, as Pseudomonas putida, which has bioremediation potential and is a strong root colonizer. The Zinc oxide Nano sanitizer perfectly ensures clean hands and forms a protective anti-bacterial layer on the skin due to its anti-microbial activity. Zinc oxide nanoparticles when formulated along with herbal substances such as Neem, Tulsi show no side effects when compared to commercially available alcoholic sanitizers. Key words: Sanitizing agent, ZnO NPs, anti-microbial activity.

Page 28: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P12

COLORECTAL CANCER AND OBESITY: ROLE OF PI3K IN TUMORINITIATION AND PROGRESSION

Authors:HarithaGoud D S.V.U.College of Pharmaceutical Sciences, S.V.University, Tirupati Abstract At present, the occurrence of Obesity has reached/reaching high levels in developed and developing countries too and since it predisposes to diabetes, hypertension, CVDs and certain cancers it has become a worldwide public health issue.Epidemiological studies have shown that, obesity-associated colon cancer accounts for 14–35% of total cancer cases. Changed food habits like consumption of pesticide/herbicide contaminated, consumption of junk foods, adulterated foodsis reported to be a major cause for this. At biochemical and molecular level, enhanced levels of insulin-like growth factor I, blood insulin, NF-kB, Akt, IL-6, leptin, TNF-α in addition to reduced adiponectin have been observed in obesity-induced colorectal cancer cases. These pathways and the signaling molecules are reported to be associated with increased activity of PI3K signal pathway. In our studies, we have isolated and evaluated certain phytochemicals likeCurcumin, Quercetin, EGCG, etc for their antiobesityacitivity. They have significantly inhibited the expression of key genes associated with obesity like TNF-α, leptin and adiponectinin both cell culture studies and diet induced obese animal model experiments. Now, the work is under progress to verify the role of above mentioned plant compounds in regulating PI3Kmediated signaling pathway and their possible role in treating colon cancer. Keywords: Carcinogenesis, Obesity, contaminated foods, PI3K,colon cancer.

Page 29: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P13

GENE THERAPY M. SNEHA, SVU COLLEGE OF PHARMACEUTICAL

SCIENCES, SV UNIVERSITY. ABSTRACT: Gene therapy involves he transfer of a therapeutic or working gene copy into specific cells of an individual in order to repair a faulty gene copy. Its main is to replace a faulty gene whose function is to cure or to favorably modify the clinical course of a condition . Mainly four important studies deals with gene therapyThree involving new approaches to cancer therapy & one involving the treatment of adenosine deaminase deficiency, are presently underway. The gene therapy process include basic steps are: - The faulty gene that causes a specific condition must be identified. The location of the affected cells in the body tissues or organs must be pinpointed. A working version of the gene must be available. By using suitable gene therapy techniques the therapeutic gene must be replaced in the presence of faulty gene. Examples for the positive recommendations for a gene therapy product by the EMA for approval and the positive trails for the treatment of ADA deficiency SCID-X1 and adrenoleukodysropy. Nevertheless, our knowledge continues to grow and during the course of time more safety data has become available that helps us to develop better gene therapy approaches. KEY WORDS:-Adrenoleukodystropy, ADA Deficiency, Therapeutic gene. REFERENCES:-Lewis Ricki, Human genetics, Biology Encyclopedia 20th century.

Page 30: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P14

REVIEW ON INVESTIGATIONAL STRATEGIES OF IMMUNOLOGY

M.Vasantha,M.Pharm(Ph.D),SVU College of Pharmaceutical Sciences, S.V.University,Tirupati.

Abstract: Immunology has its origins in the study of how the body protects itself against infectious diseases caused by microorganisms, such as bacteria, viruses, protozoa, and fungi, and also parasitic organisms, such as helminth worms.The immune system consists of two branches such as the innate immune system that utilises certain hard- wired strategies to provide a rapid, general, response when alerted by certain typical signals of infection (essentially forming a first-line of defence and the adaptive immune system that is able to develop highly specific responses (and a persistent ‘immune memory’) to target infection with extraordinary accuracy.All immune cells originate in the bone marrow, deriving from haematopoietic stem cells, but an important set of immune cells (T lymphocytes) undergo maturation in an organ known as the thymus.There is a great deal of synergy between the adaptive immune system and its innate counterpart, and defects in either system can provoke illness or disease, such as autoimmune diseases, immunodeficiency disorders and hypersensitivity reactions.The immune system is a part of body to detect the pathogen by using a specific receptor to produce immediately response by the activation of immune components cells, cytokines, chemokine’s and also release of inflammatory mediator. They modulate and potentiate the immune system. Keywords:Immunology, Innate immunity, Adaptive immunity, Immune cells, Auto immune disease

Page 31: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P15

DIABETES MELLITUS – A DEVASTATING CHRONIC METABOLIC

DISORDER N.LAKSHMI PRIYA, M.Pharmacy,

Department of Pharmaceutics. Sri Venkateswara University College of Pharmaceutical Sciences Tirupati, AndhraPradesh. Email: [email protected], Mobile: 7287828501. Abstract: Diabetes mellitus is an endocrinological and/or metabolic disorder with an increasing global prevalence and incidence. High blood glucose levels are symptomatic of diabetes mellitus as a consequence of inadequate pancreatic insulin secretion or poor insulin-directed mobilization of glucose by target cells. Diabetes mellitus is aggravated by and associated with metabolic complications that can subsequently lead to premature death. This includes the detailed explanation of diabetes mellitus in terms of its historical perspective, biochemical basis, economic burden, management interventions along with the future perspectives. Keywords: Insulin, blood glucose levels, islets of langerhans, hyperglycemia

Page 32: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P16

INVITRO EVALUATION OF ANTI-ARTHRITIC ACTIVITY IN DIFFERENT SOLVENT EXTRACTS FROM CUCUMIS SATIVUS M.Vasantha (Ph.D)(Pharmacology),S.V.U College of Pharmaceutical

Sciences,S.V.University,Tirupati. ABSTRACT Objective: Cucumis sativus is a medicinal plant & edible fruit which is used for the treatment of arthritis in folklore medicine. The present study was aimed at the investigation of anti-arthritic activity of ethanolic and aqueous solvent extracts of C. sativus by invitro models. Materials and methods: The anti-arthritic activity of C. sativus was done by invitro method of inhibition of protein denaturation and membrane stabilisation method. Solvent extracts of C. sativus was subjected to invitro methods in two concentrations : 400 and 800 μg/ml. Results: The present findings exhibited a concentration dependent inhibition of protein ( bovine albumin) denaturation and membrane stabilisation method by the two solvent extracts of C. sativus . The ethanolic extract of C.sativus (800 μg/ml )possessed significant anti-arthritic activity as compared to standard drug acetyl salicylic acid(200μg/ml). Conclusion: From the present study it can be concluded that C.sativus showed marked invitro anti-arthritic activity against the denaturation of protein and membrane stabilisation method .

Page 33: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P17

MOLECULARLY TARGETED ANTI -CANCER DRUG Introduction Cancer occurs when cell undergoes uncontrolled divison. A tumor is a mass composed of a cluster of such abnormal cells. Most cancer forms tumors, but not all tumors are cancerous. benign or noncancerous tumor do not spread to other body parts whereas malignant, or cancerous, tumor crowd out healthy cells. Molecularly targeted drugs are those which target specific molecules or molecular sites and are directly involved in growth and spread of cancerous cells. Transtuzumab used for the treatment of breast cancer in combination with other drugs. It targets HER-2 gene and downregulates it and further results in upregulation of cell cycle inhibitors p21 and p27. Gleevec which is a tyrosine kinase inhibitor ; target BCR-Abl. It is specifically used to treat chronic myelogenous leukemia (CML) and acute lymphocytic leukemia (ALL). The active sites of tyrosine kinases each have a binding site for ATP.The enzymatic activity catalyzed by a tyrosine kinase is the transfer of the terminal phosphate from ATP to tyrosine residues on its substrates, a process known as protein tyrosine phosphorylation. In myelogenous leukemia (CML) there is expression of chimeric BCL-AbL tyrosine kinases protein and many gastrointestinal stromal tumors shows the expression of c- kit growth factor receptor. Anticancer drug Imatinib binds to the ATP binding site of the receptor mentioned .therefore inhibiting the binding of ATP. CML arises by a translocation between chromosome 9 and 22 in a pluripotent hematopoietic stem cell to produce aberrant chromosome in which a part of chromosome 22 is translocated to 9 and a part of 9 and 22. Such molecular targeted anticancer drug can be used in combination with other drugs. Different molecular sites can be targeted .Target sites can be altered using such drugs which can be helpful in treating various cancer .it is hoped that better responses in blastic phase disease will be achieved in combinations with other drugs. Submitted by; Aakriti Singh, Payal Talekar, Hima S R, Kajal Rathod Ayushi Sepurwar, MSc.Biotechnology (2 yrs) Sem 2,Sri Padmavati Mahila Viswavidyalam,Tirupati

Page 34: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P18

FORMULATION OF SEAGRASS (HALOPHILA BECCARII) & GREEN

TEA (CAMELLIA SINENSIS) EXTRACT AGAINST ALCOHOL INDUCED HEPATOTOXICITY & NEPHROTOTOXICITY

M.Uma, M.Geetha, M.Vani, P. Uma MaheswariDevi Department of Biochemistry & Applied Microbiology, Sri padmavati Mahila

Viswavidyalayam, Tirupati. [email protected] ABSTRACT:

The aim of this study was to examine the protective effect of Seagrass (Halophila beccarii) & Green tea (Camellia sinensis) extract against alcohol induced Hephatotoxicity and Nephrotoxicity by using rat model. This study included 8 groups containing six rats in each group: Group A (control), Group B (Alcohol-15%), Group- C (Seagrass-2.5%), Group-D(Green tea-2.5%), Group-E(Alcohol+Seagrass), Group- F(Alcohol+Greentea), Group-G (Alcohol + seagrass + Green tea), Group- H(Seagrass+Green tea). Hepatoprotection was assessed by measuring the levels of hepatic markers including Aspartate transaminase (AST), Alanine transaminase(ALT) and antioxidant markers including Glutathione(GSH) in liver homogenate and serum. Phytochemicals screening of the extract indicate the presence of Flavanoids, Antioxidants, Saponins, Phenol compounds-Amylase inhibitors, Vitamin E & C. Elevation of AST, ALT levels along with depletion of GSH in the liver were observed in rats treated with alcohol alone compared with control. The treatment of animals with aqueous extract of Seagrass & Green tea reduced the levels of AST , ALT and increases GSH levels by compared rats treated with alcohol. Furthermore, Histopathological observations, consistent with Biochemical findings showed that Seagrass & Green tea extract treatment significantly reduced portal inflammation and infiltration of mononuclear cells pronounced by ethanol. Nephrotoxicity is assessed by the measuring the levels of creatinine and Bilirubin in serum . The treatment of animals with aqueous extract of Seagrass & Green tea reduced the levels of creatinine and Bilirubin levels by compared rats treated with alcohol. Key words: Seagrass, Green tea, Hepatotoxicity, Nephrototoxicity, AST, ALT, GSH, Bilirubin, creatinine

Page 35: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P19

IDENTIFICATION OF NOVEL ANTAGONIST AGAINST TENASCIN-C INDUCED CANCER

Lalapeta S Divya, Sivaranjani Pakala, Veena Kavali, Bhuvaneswari Poola and Amineni Umamaheswari1* Bioinformatics Centre, Department of Bioinformatics1, Sri Venkateswara Institute of Medical Sciences University, Tirupati-517507 Email: [email protected] Tenascin-C (TNC) is a large, multimodular, extracellular matrix glycoprotein member of tenascin gene family characterized by an tenascin-type epidermal growth factor (EGF)-like repeats, a series of fibronectin (FN) type III domains and a C-terminal fibrinogen-related domain. TNC has pleotrophic role in creating metastatic niche by promoting migratory and invasive cell behaviour, angiogenesis and cancer cell viability under stress that implicated in different types of cancers such as glioblastoma, breast cancer and lung cancer etc. Adverse effects of existing treatment options of antiangiogenic agents such as hemorrhage, clots in arteries, impaired wound healing and hypertension, there is an emergent need for new comprehensive treatment strategy combining with conventional treatment of cancer. Hence in the present study, TNC was selected to design novel inhibitors through virtual screening, docking, lead validation and molecular dynamics. 306 inhibitors of human TNC were searched in literature and retrieved. Grid was generated on fibronectin binding site of TNC and docked with the selected inhibitors. The best docked existing inhibitors (bicaltumide and nabumethone) are screened against 28.1 million inhouse library molecules resulted 6000 TNC inhibitor library. Rigid receptor docking (HTVS, SP and XP) of Schrodinger software suite v2019-1 (Maestro v11.1) was utilized for identification of the best leads against TNC. Among them one lead showed better binding affinity (-49.856 kcal/mol) and the least XP Gscore of -5.09 kcal/mol with TNC. Then stability of the best lead and bicaltumide in physiological conditions were assessed by performing 100 ns MD simulations. Lead displayed two hydrogen bonds with Trp-45 and His-48, van der Waal’s interaction with Trp-25, Asp-43, Leu-44, His-50, Ser-52 and pi-pi stacking interactions with Tyr-51 of TNC binding site residues with integrins. Thus, the proposed lead provides no space for binding with integrins which leads to the inhibition of the pro-metastatic and angiogenic activities by regulating the intracellular pathways such as Notch and Wnt in different cancers. The proposed lead showed better binding orientation and having favorable ADME/T properties than the bicaltumide which is an approved drug for prostate cancer. Hence, the proposed lead can be used for designing the best TNC antagonist to treat cancer if synthesized and tested in animal models. Key words: Tenascin-c, Angiogenesis, Metastasis, Rigid receptor docking, Molecular dynamic simulations.

Page 36: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P20

DESIGN AND CHARECTARISATION OF ORODISPERSIBLE DOSAGE FORMS INCORPORATING DRUG NANOPARTICLES

Dr. A. DEEVAN PAUL*, Academic consultant, SVU College of Pharmaceutical sciences, SV University, Tirupati Email: [email protected] ABSTRACT The routes of absorption via the highly vascularized buccal mucosa allow the substance a more direct access to the blood circulation, thus providing direct systemic administration medically, sublingual drug administration is applied in the field of cardiovascular drugs, steroids, some barbiturates, and enzymes. The Orodispersable route usually produces a faster onset of action than orally ingested and the portion absorbed through the sublingual blood vessels bypass the hepatic first-pass metabolic processes. Nanoparticulate engineering enables poorly soluble drugs to be formulated as particles alone, or with a combination of pharmaceutical excipients.Amlodipine besylate is a dihydropyridine calcium channel antagonist originally introduced for the treatment of hypertension, certain types of angina and coronary heart failure. It is extensively (about 90%) converted to inactive metabolites via hepatic metabolism with 10% of the parent compound and 60% of the metabolites excreted in the urine. Anti-solvent precipitation method can be used as an effective tool for preparation of Nanosized formulations. The study conclusively demonstrated significant results for Lyophilized Amlodipine besylate orodispersible Nano particulate dosage form tablet.Amlodipine nanoparticles prepared by this method showed significant improvement in aqueous solubility as well as dissolution characteristics which may significantly improve its oral bioavailability. Keywords: Lyophilization, Nano particulate dosage form, bio availability, re- vascularisation.

Page 37: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P21 ANTICANCER ACTIVITY OF NATURAL COMPOUNDS FROM PLANT

AND MARINE ENVIRONMENT Abstract: This paper describes the substances of plant and marine origin that have anticancer properties. The chemical structure of the molecules of these substances, their properties, mechanisms of action, their structure–activity relationships, along with their anticancer properties and their potential as chemotherapeutic drugs are discussed in this paper. This paper presents natural substances from plants, animals, and their aquatic environments. These substances include the vinca alkaloids, mistletoe plant extracts, podophyllotoxin derivatives, taxanes, camptothecin, combretastatin, and others including geniposide, colchicine, artesunate, homoharringtonine, salvicine, ellipticine, roscovitine, maytanasin, tapsigargin, and bruceantin. Compounds (psammaplin, didemnin, dolastin, ecteinascidin, and halichondrin) isolated from the marine plants and animals such as microalgae, cyanobacteria, heterotrophic bacteria, invertebrates (e.g., sponges, tunicates, and soft corals) as well as certain other substances that have been tested on cells and experimental animals and used in human chemotherapy. Keywords: natural compounds; anticancer properties; substances from marin VARUVA. ASHOK KUMAR M. PHARMACY [PHARMACOLOGY] SRI VENKATESWARA COLLEGE OF PHARMACEUTICAL SCIENCES SRI VENKATESWARA UNIVERSITY, TIRUPATI, CHITTOOR ,517502.

Page 38: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P22

COSMOCEUTICAL VALUE OF HERBAL EXTRACTS IN NATURAL WITH NOVEL TECHNOLOGIES IN ANTI- AGING

M LAKSHMI, A DEEVAN PAUL SVU College of Pharmaceutical Sciences, SV UNIVERISTY

ABSTRACT: Herbal cosmetics are formulatedusing different cosmetic ingredients to form the base in which one or more herbal ingredients are used to cure various skin ailments. The name itself suggests that herbal cosmetics are natural and free from all harmful synthetic chemicals often to prove efficacy inskin sensitisations, in comparison with other beauty products the natural cosmetics are safe to use. Cosmeceuticals are cosmetics in pharmaceutical hybrid products intended to improve the health and beauty of the skin by providing a specific result, ranging from acne, control and anti-wrinkle effects to skin protection by introducing the novel technologies in anti-aging. Cosmeceuticals have medical benefits that affect the biological functioning of skin depending upon the type of functional ingredient, by these natural cosmeceuticals improves the texture of skin by boosting collagen growth by eradicating harmful effects if free radicals in maintaining keratin structure in good condition for healthier skin. There are numerous natural herbs available with different uses in cosmetic preparations for skin, hair and anti-oxidant activities. The current review highlights the importance of herbal cosmetics in herbs with their advantages over synthetic counterparts Keywords: Herbal Cosmetics, Cosmeceuticals, Keratin, Skin ailments.

Page 39: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P23 NUTRACEUTICAL PRODUCT FOR POST MENOPAUSAL WOMEN

D. Vishnu Varsha*,Prof. P. Suvarnalatha Devi, T. SwetaTejaswi

Department of biochemistry and applied microbiology, Sri Padmavathi Mahila Visva Vidhyalayam, Tirupati. [email protected] ABSTRACT Menopause is one of the common most significant events and result in a variety of physiological changes that eventually impact a women’s life. During this stage, ovaries stop releasing eggs, as well as estrogen and progesterone hormones. These low levels of estrogen and progesterone raise risk for certain health problems like osteoporosis, cardiovascular diseases, vaginal dryness, mood swings, endometrial hyperplasia etc. constitutes postmenopausal syndrome.I want to prepare a new nutraceutical product with a powder formulation containing Ginkgo Balibo, almonds, walnuts, flaxseeds, lentils, soybean, wheat berries, collard greens, turnips, mustard green which improves cardiovascular health, provide phytoestrogens and increase calcium respectively. Nutraceuticals are food or part of a food that provides medical or health benefits, including disease prevention and/or treatment. Nutraceutical has an advantage over the medicinal product as it avoids side effects, improves health benefits, have natural dietary supplements, improves human health and is far more economically affordable. Keywords: postmenopausal syndrome, nutraceutical products, endometrium hyperplasia, phytoestrogens and calcium.

Page 40: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P24

DIAGNOSIS OF DENGUE

N. JWALITHA

SVU COLLEGE OF PHARMACEUTICAL SCIENCES Abstract: Dengue is a mosquitos -borne viral disease that has rapidly spread in all regions. Dengue disease causing virus is transmitted by female mosquitos mainly of the species aedesaegypt . These mosquitoes also acts as a vectors of chikangunya,yellow fever. National dengue day is may 16. Severe dengue was first recognizes in the 1950 during dengue epidemics in the Philippines and thiland. One case study indicates 390 million dengue virus infections per year 284 million people . Another case study reveals that 3.9 billion are at risk of infection with dengue viruses. The virus is transmitted through the bites of infected female mosquitoes to a new host. Incubation period is 8-12 days. Dengue is a severe ,flu like illness that affects about infants, young children’s and adults Symptoms usually last for 2-7 days. Dengue should be suspected when a high fever 40C/140F degrees. It also accompanied by symptoms like headache, nausea, vomiting, muscle and joint pain, restlessness, blood in vomit. Several methods can be used for diagnosis of dengue. These include virological tests, serological tests. There is no specific treatment for dengue fever. Drugs used for the treatment for dengue fever. Drugs used for the treatment are pain killers, fever reducers , NSAIDS. Prevention and control of the dengue by reducing the transmission of virus & by taking measures to avoid breeding around the houses and work places Key words: Nausea, vomiting, muscle and joint pain,Blood in vomit, rest

Page 41: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

25

INHIBITION OF BREAST CANCER CELL PROLIFERATION VIA NOTCH SIGNALLING PATHWAY BY GINGER EXTRACT

Kodavala Sireesha and P.V.G.K.Sarma Cell culture Laboratory, Department of Biotechnology, Sri Venkateswara Institute of Medical Sciences, Tirupati. Abstract Breast cancer is one of the most prevalent cancers in women throughout the globe. Drugs against it have a significant value. Ginger has received extensive attention because of its antioxidant, anti inflammatory and anti tumour activities. In the present study the effect of ginger extract on Breast cancer cells was assessed. MTT assay was done to evaluate the cytotoxic effect of ginger extract on two breast cancer cell lines MCF-7 (Michigan Cancer Foundation-7) and BCFNA (Breast Cancer Fine Needle Aspirated). MCF-7 cells and BCFNA cells were inhibited with an IC50 value of 300µg and 50µg respectively. Zymography was done to check the release of metallo proteases that has capacity to degrade the gelatine present in the gel and form clear zone. After the treatment of ginger extract the cell lines failed to clear the gel matrix clearly explaining the suppression of cell proliferation. Hairy and enhancer of split (HES1), a transcription factor whose expression is initiated by the Notch signaling pathway, is a repressor of cell cycle inhibitors. Activation of Notch pathway leads to increase in expression of HES1 gene leading to Breast cell proliferation. Expression of HES1 gene was studied in ginger extract treated and untreated MCF7, BCFNA cell lines. Low expression in treated cells and high expression in untreated cells suggests that ginger extract has inhibited the proliferation of Breast cancer cells. These results explain dietary inclusion of ginger would go long way in preventing breast cancers.

Page 42: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P26 Ammireddy raja Abstract: Neuroprotection is the preservation of the structure and function of neurons from insults arising from cellular injuries induced by a variety of agents or neurodegenerative diseases (NDs). The various NDs including Alzheimer’s, Parkinson’s, and Huntington’s diseases as well as amyotropic lateral sclerosis affect millions of people around the world with the main risk factor being advancing age. Each of these diseases affects specific neurons and/or regions in the brain and involves characteristic pathological and molecular features. Hence, several in vitro and in vivo study models specific to each disease have been employed to study NDs with the aim of understanding their underlying mechanisms and identifying new therapeutic strategies. Of the most prevalent drug development efforts employed in the past few decades, mechanisms implicated in the accumulation of protein-based deposits, oxidative stress, neuroinflammation, and certain neurotransmitter deficits such as acetylcholine and dopamine have been scrutinized in great detail. This review is designed to give a brief description of a number of neurodegenerative diseases, the most important pathological events associated with the diseases, and an overview of therapeutic options with some popular plant-derived neuroprotective agents.

Page 43: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P27

ANTI-ULCER ACTIVITY OF FICUS RELIGIOSA LEAF ETHANOLIC EXTRACT

G.Gowthami, M.Pharmacy , S.v.u, Tirupati. ABSTRACT: Objective: To evaluate the anti-ulcer activity and acute toxicity of Ficus religiosa (F. religiosa) leaf ethanolic extract in animal models. Methods: Anti-ulcer activity of F. religiosa ethanolic extract (250 and 500 mg/kg body weight) was studied on stress induced ulcer animal models. Ranitidine was used as standard. The anti-ulcer activity of F. religiosa was evaluated with the help of ulcer area and histopatholgical examination. Preliminary phyto-chemical screening and acute toxicity studies of F. religiosa also carried out. Results: Results showed that the extract treatments prevented ulcer area and gastric secretion in a dose-dependent manner. Administration of 2000 mg/kg extract did not show any acute toxicity in albino mice. Preliminary phytochemical analysis identified the presence of flavonoids in the ethanolic extract of F. religiosa. Conclusions: The extract is non-toxic even at relatively high concentrations. The anti-ulcer activity is probably due to the presence of flavanoids. KEYWORDS: Ficus religiosa, Flavonoids, Ethanolic extract, Ulcer area, Ranitidne.

Page 44: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P28

MANAGEMENT OF RHEUMATOID ARTHRITIS Muneer Basha SVU, Tirupati.

ABSTRACT :

Rheumatoid arthritis (RA) can be defined as an autoimmune inflammatory disease which is represented in about1% of the world population and occurs in the middle age with increasing frequency in older population.RA has several types which can be caused by joint inflammation,bone or joint damage. Scientists were unable to determine the causes of such inflammation until few years ago when some evidences or clues were unveiled that involved mainly genetics and environmental factors. Although there is no known complete cure of RA,treatment aim to achieve several goals which are pain relief, slow joint degeneration and improve patient well-being. To achieve that, several approaches could be applied including lifestyle, medication, routine monitoring and surgery. Keywords: RA, DMARDs, Methotrexate, NSAIDs, corticosteroids.

Page 45: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P29

ANTIBACTERIAL AND ANTIFUNGAL ACTIVITIES FROM LEAF EXTRACTSOF CASSIA FISTULA

Vijaya lakshmi, S.V.U, Tirupati. Abstract This study was carried out with an objective to investigate the antibacterial and antifungal potentials of leaves of Cassia fistula Linn. The aim of the study is to assess the antimicrobial activity and to determine the zone of inhibition of extracts on some bacterial and fungal strains. In the present study, the microbial activity of hydroalcohol extracts of leaves of Cassia fistulaLinn. (an ethnomedicinal plant) was evaluated for potential antimicrobial activity against medically important bacterial and fungal strains. The antimicrobial activity was determined in the extracts using agar disc diffusion method. The antibacterial and antifungal activities of extracts (5, 25, 50, 100, 250 μg/ml) of Cassia fistulawere tested against two Gram-positive—Staphylococcus aureus, Streptococcus pyogenes; two Gram-negative—Escherichia coli, Pseudomonas aeruginosahuman pathogenic bacteria; and three fungal strains— Aspergillus niger, Aspergillus clavatus, Candida albicans.Zone of inhibition of extracts were compared with that of different standards like ampicillin, ciprofloxacin, norfloxacin, and chloramphenicol for antibacterial activity and nystatin and griseofulvin for antifungal activity. The results showed that the remarkable inhibition of the bacterial growth was shown against the tested organisms. The phytochemical analyses of the plants were carried out. The microbial activity of the Cassia fistulawas due to the presence of various secondary metabolites. Hence, these plants can be used to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals research activities. Keywords: Cassia fistula,in vitroantibacterial activity, antifungal activity, secondary metabolites

Page 46: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P30 A REVIEW ON LIPID BASED CARRIER DRUG DELIVERY SYSTEM:

LIPOSPHERES Nagavani

ABSTRACT: Lipospheres are lipid-based water dispersible solid particles of particle size between 0.01 and 100 µm in diameter composed of a solid hydrophobic lipid core (triglycerides), stabilized by a layer of Phospholipid molecules embedded in their surface. Lipospheres delivery system is a suitable carrier system for the delivery of both hydrophobic and hydrophilic drugs. Much of research is now focused on using lipids as novel carriers for drug moieties. Lipid based drug delivery systems like solid lipid nanoparticles, lipospheres (LS) are being developed as substitutes for “polymer based delivery systems” due to the increasing toxicity related concerns of monomers after intracellular processing of polymers and attractive benefits offered by lipids as carriers. Lipospheres are new type of drug delivery system developed mainly for parenteral system. As most of the recent drugs discovered (nearly 40-45 %) are lipophilic in nature having poor water solubility and less bioavailability. This article explains about formulation of lipospheres, factors influencing the quality attribute of lipospheres, mechanisms behind drug loading, evaluation of lipospheres and challenges in the development of lipospheres are discussed in detail. Key words: Lipospheres, Phospholipid, hydrophobic, hydrophilic drugs, polymers.

Page 47: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P31 TARGETING RAF-MEK-ERK BY NOVEL LEAD THROUGH IN SILICO

APPROACHES

Vankadoth Umakanth Naik*, Sandeep Swargam, Katari Sudheer Kumar and Amineni Umamaheswari*

Bioinformatics Centre, Department of Bioinformatics, SVIMS University, Tirupati-517507 *Presenting author **Corresponding author: [email protected] Abstract:

Mitogen-activated protein kinases (MAPK) are serine/threonine kinases that act as secondary messengers or effectors for cell proliferation and cell survival in signal transduction pathways. MAPK1/ERK2 and MAPK3/ERK1 are the two MAPKs phosphorylate more than 160 different substrates during signal transduction. MEK activity on ERK results in dual phosphorylation of Thr-Glu- Tyr motif of ERK1 T202/Y204which activates ERK1 to phosphorylate various transcription factors that promotes tumorigenesis through Ras-Raf-MEK- ERKpathway. Three e-pharmacophores (ADDDR, AADRR and ADRR) were generated from the three diverse co-crystal structures of ERK1 (2ZOQ-5ID, 4QTB-38Z and 6GES-6H3). Virtual screening of three e-pharmacophores, vemurafenib and trametinib against >21 million inhouse-library small molecules yielded 5,000 similar hits, the hits were subjected to rigid receptor docking over ERK1 (4QTB) through high throughput virtual screeningrefinement of 10% (500 hits), standard precision of 10% (50 hits) and extra precision of 10% resulted 5 ligands with docking and binding free energy scoring functions. Among the 5 ligands, one compound scored better than crystal ligands and published inhibitors (vemurafenib and trametinib) was selected by comparing docking and binding free energy score. Quantum polarized ligand and induced fit docking results also favoured the top ranked compound as the best lead over the existing ligands. The dynamic parameters like root mean square deviations, fluctuations, protein ligand contacts and energies infers that the lead-ERK1 complex was more stable compared than crystal ligand (38Z) in 100 ns molecular dynamics simulations. The proposed lead inhibits ERK’s ATP binding site, thereby arresting phosphorylation or activation of various transcription factors that induces cell proliferation during oncogenesis. Keywords: MAPK, E-pharmacophores, Virtual screening, Docking and Molecular dynamics

Page 48: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P32

CORE HOPPING-BASED DESIGNING OFEFFECTUAL INHIBITORS AGAINSTLIPOPOLYSACCHARIDE (LPS)BIOSYNTHESIS

USINGCONSERVED TARGET D-SEDOHEPTULOSE 7-PHOSPHATE ISOMERASE

ChiranjeeviPasala*, RavinamadhulithaNalamolu, Umamaheswari Amineni**. *Presenting author: [email protected]; ** Corresponding author: [email protected] Abstract:

Helicobacter pylori, infects 50% of world human population and became a causing agent of gastric cancer, 5th most common neoplasm and 3rd most deadly cancer (GLOBOCAN-2018).In addition to adverse drug affects, drug resistance in Helicobacter pyloriis rampant as intemperate and inappropriate use of antibiotics led to emerging of multidrug-resistant strains and limiting the efficacy of the pre-existing medication.The drug discovery process becomes a formidable challenge for the development of effective therapeutics with target-specific action and without side affects. Hence, we carried out broad spectrum target analyses against 53 H. pylori strains and identified D-sedoheptulose 7-phosphate isomerase (GmhA) enzyme as a conserved (common) target involved in first step of the subpathway of lipopolysaccharide core region biosynthetic processessential for the cell wall formation.Inthe study,we developed quaternary- structure of the target and was deposited in PMDB database with < 3% stereochemical check failure (PM0082288).In lead-optimization campaign, substrate (D-sedoheptulose 7-phosphate) and existinginhibitors-basedshape screening against >21 million compounds, multitier docking (RRD, QPLD, andIFD), MM/GBSA, enrichment factor(ROC metrics) calculations and parent lead-based CoreHopping method resulted ninenovel leads having better binding strengths with active tunnel residues and good pharmacological (ADME/Tox) properties than previous inhibitors. Long range (300ns) molecular dynamics simulations for substrate-GmhA and lead1-GmhA unveiled the system consistency nature in physiological milieu. Thereby, these designed scaffolds could be suggested to propose as competitive inhibitors against GmhA to curtail drug resistance of H. pylori strains. Key words:H. pylori, GmhA, multitier docking, MM/GBSA, core hopping, dynamics simulations

Page 49: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P3 THE PROTECTIVE ROLE OF BRONCO-T IN REGULATION OF LIPID

PROFILE AND GENE EXPRESSION IN RATS ON EXPOSURE TO FORMALDEHYDE.

S. Payani, B. Sujatha, C. Mamatha, C. Chandraprakash and M. Bhaskar* Department of Zoology, S.V.University, Tirupati-517502, A.P. *Corresponding Email: [email protected] Abstract

Formaldehyde is one of the imprudent pollutants, and has extensive range of household (flooring materials, wood-based panels, paper products, etc.) and industrial sources. It is a toxic compound for living cells due to its nonspecific reactivity with proteins and nucleic acids. Bronco-T was polyherbal plant product which is consists of five herbal plants such as Cinnamomum tamala, Solanum xanthocarpum, Albizialebbeck, Justicia adhatoda and Yastimadhu. These plants are extensively used to treat many diseases. The present study was carried out to evaluate the effect of formaldehyde exposure on lipids in blood and gene expression in rat lung. Bronco-T a poly-herbal formulation was used as the remedy. In this experiment thirty adult male albino Wistar rats were used for the study and subdivided in to five groups consist of 6 rats in each group. Group-I served as control and the other 4 groups such as II, III, IV and V are considered as experimental. The control and experimental rats were maintained for 21 days period. Group III, IV and V were exposed to 40% formaldehyde for one hour and later treated with Bronco-T (Group- IV) and salbutamol drug (Group-V) for one hour. In the present investigation, the lipid profile (LDL, HDL, VLDL and SGOT) of blood and gene expression in lung (A1AT, IL-4) were analysed. Alpha1Antitrypsin (A1AT) is mainly produced in the liver and reaches the lungs by diffusion from the circulation and functions to protect the lung against proteolytic damage (e.g., from neutrophil elastase). Interleukin-4 is expressed by lung macrophages and T-lymphocytes in this model is consistent with the concept that IL-4 is a key cytokine of the Th2 type immune response. On exposure to Formaldehyde (Group-III), the rats showed significant decreased levels of lipid profile in blood compared to control rats and increased levels were observed in group –IV and group-V, but Bronco T treated rats were showed improved levels when compared to standard drug. In lungs A1AT expression was decreased and IL-4 expression was increased in formaldehyde exposed group than Bronco -T and salbutamol treated rats. An expression level of A1AT was increased and IL-4 was decreased in bronco-T treated rats compared to salbutamol. The result indicates that lipid levels and gene expression are regulated effectively by Bronco-T than standard drug. So Bronco-T treatment may be a suitable remedy for formalin induced occupational diseases. Key words: Bronco-T, Alpha1Antitrypsin, Interleukin-4, formaldehyde, polyherbal, metabolic markers, salbutamol.

Page 50: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P34

ANTI-CANCER PROPERTY OF CURCUMIN E.L. Samundeshwari and P.V.G.K Sarma

Cell culture Laboratory, Department of Biotechnology, Sri Venkateswara Institute of Medical sciences, Tirupati ABSTRACT Breast cancer is the leading cancer related to death among female in the world. Natural products have been used for the treatments for cancer due to its wide range of biological activity and low toxicity. The present study examined anti-proliferation activity of Curcumin and its effect on the apoptosis of breast cancer cell like MCF-7 and BCFNA. In this study in-vitro assay such as MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide), migration assay, Soft agar assay, gelatin zymography were performed to determine migration of cells, the number of colonies formed, anti-proteases activity of Curcumin in breast cancer cells and quantitative real time PCR is used for the detection of MCL-1 gene expression. Based on MTT Assay 50% of cells were inhibited by increasing the concentration to 800µM in BCFNA and 620 µM in MCF-7 compared to control. Similarly MCL-1 gene expression decreased with increasing Curcumin concentration shows 100 folds difference compared to control. Soft agar assay results after incubation the cells with IC50 value of drug number of colonies were decreased when compared to control. Our present study findings indicate Curcumin significantly inhibited the growth of BCFNA and MCF-7 by inducing Apoptosis. Here Curcumin bind to the receptors like EGFR and cytokine receptor and they induced signals to activate the JAK and STAT pathway which regulates MCL-1 gene expression cause the release of Cytochrome - C from Mitochondria. By promoting Curcumin as dietary ingredient will be long way in preventing occurrence of breast cancer. KEY WORDS: Curcumin, Anti-apoptosis, MCF-7, BCFNA, JAK- STAT Pathway.

Page 51: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P35

ANTI ULCER ACTIVITY OF POLY HERBAL FORMULATION OF ALOE VERA AND OCIMUM SANCTUM

C.Girish, Prof. Ch. Appa Rao

S.V.U.College of Pharmaceutical Sciences, Sri Venkateshwara University, Tirupati - 517501. A.P. ABSTRACT The lesion of peptic ulcer disease is a disruption in the mucosal layer of the stomach or duodenum. Recent researchers have discovered a number of newer risk factors regulating the development of disease which are not addressed properly by the current clinical therapies. In this study polyherbal formulation was prepared consisting of Aloe vera and Ocimum sanctum. Suitable extracts depending on effective chemical composition was prepared and studied on three antiulcer models, namely aspirin induced ulcer model, Ethanol induced ulcer model and Histamine induced ulcer model. This polyherbal formulation significantly reduces ulcer index. All results show that polyherbal formulation show gastric healing property by multiple mechanisms. Many drugs are there in the market to treat the ulcer, but they are having lot of adverse effects. The present study reveals, by using combination of herbal drugs we have proved that these are the effective alternatives of chemical drugs. Keywords: Peptic ulcer, Aloe vera, Ocimum sanctum, Omeprazole, Histamine, Ranitidine.

Page 52: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P36

IDENTIFICATION AND CHARACTERIZATION OF EPO-R GENE IN PATIENTS WITH ERYTHROCYTOSIS

E.L.Samundeshwari, Dr.Chandra Sekar. C, Dr.P.V.G.K.Sarma Medical Genetics Laboratory, Department of Biotechnology, Sri Venkateswara Institute of Medical Sciences, Tirupati. ABSTRACT Primary familial and congenital Erythrocytosis is a rare autosomal dominant inherited disorder with isolated proliferation of the Erythroid progenitor cells leading to Erythrocytosis. It is caused by deregulated red blood cell production where erythrocyte overproduction results in elevated hemoglobin and hematocrit levels. Family Studies revealed secondary cause were ruled out that Erythropoietin (EPO) levels were normal or low or resulted from mutations found in EPOR gene lead to the truncation of the C-terminal part of the protein which plays a key role in the modulation of the cell response to EPO stimulation. In order to understand the involvement of mutations in the EPOR gene, suspicious family with Erythrocytosis was taken genomic DNA was isolated, Exon 8 of EPOR gene was amplified, sequenced (using Sanger sequencing) and Endogeneous Erythroid Colony Forming assay was performed. Our present finding showed mutation c.1013 G>A in EPO-R gene and CFU-E is positive for patients with mutation in EPO-R Gene, which indicates abnormal EPO production due to variations in EPOR gene, probably this could be the causative reason for the congenital Erythrocytosis in this patients. To our knowledge this mutation is the first observed in Indian population, which concludes these patients were diagnosed with Congenital Erythrocytosis. KEY WORDS: Missense mutation, Congenital Erythrocytosis, EPOR gene.

Page 53: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P37

COUMARIN AND CINNAMALDEHYDE IN CINNAMON INHIBITS IN VITRO PROLIFERATION OF BREAST CANCER CELL LINES

Kattaru Surekha and P.V.G.K.Sarma Cell culture Laboratory, Department of Biotechnology, Sri Venkateswara Institute of Medical Sciences, Tirupati. Abstract Cancer is one of the leading causes of death worldwide and the prevalence of cancer has been considerably increased. The consequence of this issue has attracted further attention to discover novel therapies to prevent and treat cancers. In recent years, natural compounds draw a major attention for their potent anti-neoplastic activity. Among those Cinnamon is one of the natural components showing a wide range of activities including anti-cancer activity. According to previous studies, drugs used to treat cancer have shown significant boundaries. Therefore, the role of herbal medicines alone or in combination with chemotherapy drugs has been enormously studied in cancer treatment. In our study the anti- cancer activity of Cinnamon is explored by treating breast cancer cells (BCFNA and MCF-7 cell lines) with increased concentration of Cinnamon extract and analyzed the results through cell counting where 98.9% of growth inhibition was observed and 1.43% of viable cells through cell viability. Results of soft agar assay demonstrated significant reduction in the number of colonies and Cinnamon reduced the migration of breast cancer cells in a considerable manner determined through scratch assay. Remarkable decrease in the expression of matrix metallo proteinases was demonstrated through Gelatin zymography. MTT assay results correlated with qPCR analysis showing down regulation in the expression of CycD1 and HER2 which are enormously expressed in cancerous condition. The expression of CycD1 and HER2 in breast cancer cells is down regulated mainly through the active compounds like coumarin and cinnamaldehyde from Cinnamon in which HER2 acts on matrix metallo proteinases and up regulates the anti-neoplastic activity while PARP1 enhances the apoptosis leading to the inhibition of breast cancer cell growth.

Page 54: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P38 EFFECTS OF ADEQUATE TREATMENT OF HYPERTHYROIDISM ON

GLYCATED HEMOGLOBIN LEVELS IN NONDIABETIC SUBJECTS WITH HYPERTHYROIDISM

Mani Deepthi Dasari, Suresh V, Alok Sachan

Department of Endocrinology, Sri Venkateswara Institute of Medical Sciences, Tirupati Corresponding author: Suresh V Aims and objectives: HbA1c levels may not truly reflect the level of ambient glycemia in hyperthyroidism due to changes in RBC turnover . Material and Methods: Adult patients with overt hyperthyroidism who were non-diabetic on an oral glucose tolerance test (OGTT) were assessed for HbA1c and reticulocyte counts at baseline and three or more months after achievement of near euthyroid status on carbimazole. Results: In 36 patients completing follow-up; after achieving euthyroidism, there was improvement in the hemoglobin from 12.19 ± 1.25gm/dl to 12.71±1.39gm/dl (p=0.004) along with an improvement in the packed cell volume. This was accompanied by a non-significant fall in the reticulocyte percentage from 1.4±1.1% to 1.2±0.9% (p=0.59). Mean HbA1c at baseline was 5.20±0.37%.After achievement of euthyroid status the HbA1c has risen to 5.35 ±0.45% (p=0.025). This resulted in a rise in proportion of patients with prediabetes based on HbA1c (> 5.7%) from 8.3% to 27.7% (p=0.016).There were no corresponding changes in fasting or post OGTT plasma glucose values (p>0.05). Conclusion: Thus HbA1c is thus falsely low in relation to glycemia in patients with hyperthyroidism and under-diagnoses dysglycemia . It should not be employed as a test to make a diagnosis of diabetes or prediabetes in patients with hyperthyroidism. Only OGTT should be used for this purpose.

Page 55: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P39

IN VITRO ANTIOXIDANT AND ANTIDIABETIC ACTIVITY OF CUMINIC ACID

Chinthirla Partha Sarathi1, A. Rajasekhar1, P. Muni Rajeswari1, N. Vedasree 1, Ch. Appa Rao*

1 Department of Biochemistry, Sri Venkateswara University, Tirupati-517102. Abstract Diabetes mellitus is a chronic metabolic disorder, caused due to the Insulin deficiency and/or its insufficiency. It is one of the leading causes of deaths globally. As Oral hypoglycemic agents (OHA) usage causes one or more side effects, Phytochemicals can be used as alternative herbal medicine to treat Diabetes mellitus with little or no side effects. In the current study Cuminic acid (4-Isopropyl benzoic acid) is used to evaluate its In vitro antioxidant and antidiabetc activity. Different doses of Cuminic acid were used to analyze the free radical scavenging effect by DPPH, H2O2 & NO methods. Alpha amylase and alpha glucosidase inhibitory effects of Cuminic acid were also studied using various doses of Cuminic acid. Glucose diffusion inhibitory assay was also carried using Dialysis bag. The result of the antioxidant study at 200µg/ml Cuminic acid showed highest free radical scavenging activity in all the methods in comparison to the standard Ascorbic acid. The results from In vitro Alpha amylase and Alpha glucosidase enzyme inhibitory studies with Cuminic acid, showed significant increase in antidiabetic activity indirectly with increasing concentrations of Cuminic acid from 50µg/ml to 200µg/ml. The In vitro antidiabetic activity of Cuminic acid was found to be equivalent to the standard drug acarbose. The result of Glucose diffusion inhibitory assay also proved to be significant compared to the standard drug metronidazole. Based on the results of the study it is concluded that Cuminic acid has potent In vitro anti oxidant and antidiabetic activity. Key words: Diabetes mellitus, Cuminic acid, Alpha Amylase and Alpha Glucosidase, Acarbose, Metronidazole.

Page 56: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P40 In Vitro Anti diabetic and Antioxidant activity of root tubers of Cyanotis

tuberose 1Nalluri Vedasree, 2Kotha Peddanna, 1Allagadda Rajasekhar, 1Chippada Appa Rao.

1Department of Biochemistry, Sri Venkateswara University, Tirupati, Andhra Pradesh, India. 2Research Centre in Chinese Medicine and Acupuncture, Asia University, Taichung, Taiwan Abstract:

Diabetes is a chronic metabolic disease that is showing an alarming increase in prevalence in developing countries such as India. Diabetes mellitus results from shortage or lack of insulin secretion or reduced sensitivity of the tissues to the insulin. Usage of Oral hypoglycemic agents causes several side effects, so there is a need for new therapeutic agent with lower side effects. Ethnomedicinal plant use had shown promise for the development of cheaper, cost-effective antidiabetic agents with fewer side effects. Cyanotis tuberosa is one of such medicinal plant which is traditionally used as ethnomedicine for curing several ailments in India. The objective of the present study was to evaluate the In vitro antidiabetic activity of root tubers of Cyanotis tuberosa. Different doses of solvent extracts (hexane, ethyl acetate, methanol and aqueous) made by soxhlation were evaluated for invitro α-glucosidase and α-amylase enzyme inhibition assays. Hexane extract at the dose of 750mg/kg.bwt had shown maximum (80%) inhibition effect on α-glucosidase and α-amylase which was comparable to that of the standard drug Acarbose (85%) when compared to the other extracts. The plant extracts were also examined for its antioxidant activities by using free radical 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging method,H2O2 and NO method. The study revealed that hexane extract possessed a very good antioxidant activity. Thus in conclusion our findings suggest that hexane extract had a potent invitro antidiabetic and antioxidant activity.

Page 57: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P41 IN-VITRO ANTIDIABETIC ACTIVITY OF CREPIDIUMACUMINATUM

ON 3T3L1 CELL LINE K.R.Padma,N. Gayathri and J. Ramadevi N. Lakshmi Manasa

Department of Biotechnology, Sri Padmavati Mahila VisvaVidyalayam (Women’s University), Tirupati, Andhra Pradesh, India. Abstract Background: Diabetes mellitus is activated due to insufficiency in assemblage of insulin by the pancreas, or by the ineptness of the insulin formed. It is a global problem and number of those affected is augmenting day by day. The usages of plants parts belonging to the Orchidaceae family of flowering plants revealed antidiabetic property. Aim and Objective: The present study was aimed to investigate antidiabetic activity of methanolic leaf extract of C. acuminatum using invitro techniques. Materials and Methods: The leaf extract of C. acuminatum was subjected to In- vitro assessment of α-Amylase inhibition, α-glucosidase reduction; flow cytometry based cellular GLUT4 expression and Glucose translocation studies. Results: The results showed that the standard drug Acarbose exhibited 85.57% at 500𝜇g/mL concentrations respectively, and IC50 value for Acarbose was found to be 42.54±0.93𝜇g/mL in comparison with C. acuminatum extract revealed 72.81% at 500 𝜇g/mL concentrations, respectively, and its IC50 was found to be 236.81±4.30 𝜇g/mL. On the other side the α-glucosidase inhibitory activity by the plant extract revealed 70.95% at 500 𝜇g/mL concentration, with its IC50 found to be 252.43±1.29 𝜇g/mL. GLUT4 expression and Glucose uptake was high in C. acuminatum leaf extract treated cells, revealing its anti-diabetic property. Conclusion: The cytotoxicity test by MTT assay as well as GLUT4 expression studies in comparison with standard drugs available in market clearly revealed the uptake of glucose. Hence, C. acuminatum could be recommended as a potential source of anti-diabetic agents. Keywords: Crepidium acuminatum, 3T3L1-cell line, antidiabetic activity, Orchidaceae, GLUT4, Glucose uptake, Flow cytometry

Page 58: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P42 ENZYMATIC AND NON ENZYMATIC ANTIOXIDANT ACTIVITY OF

THE ACTIVE FRACTION OF THE ROOT TUBERS OF A. GONOCLADOS (AGAF)

1Allagadda Rajasekhar, 2Kotha Peddanna, 1Nalluri Vedasree, 1Chinthirla Partha Sarathi, 1Chippada Appa Rao.

1Department of Biochemistry, Sri Venkateswara University, Tirupati, Andhra Pradesh, India. 2Research Centre in Chinese Medicine and Acupuncture, Asia University, Taichung, Taiwan Abstract Asparagus gonoclados is a endemic plant to Tirupati region which have a potent antihyperglycemic and antihyperlipidemic activity. The aim of the present study was to evaluate the enzymatic and non enzymatic antioxidant activity of the active fraction of the root tubers of A. gonoclados (AGAF). The male wistar rats aged 3 months with body weights 180 -220 g were divided in to five groups, and strepotozotocin induced diabetic rats were treated with AGAF at the dose of 50 mg/Kg b.wt/day for 45 days. After treatment with AGAF for 45 days there was a significant decrease in Plasma and Liver TBARS (Lipid peroxides) and increase in the activities of GPx, SOD and GST in both tissues of diabetic rats. Similar effects were observed in the Glibenclamide Treated diabetic rats. Normal and Normal Treated rats had maintained normal levels of activities of these enzymes. The administration of AGAF to the diabetic rats has increased the levels of non enzymatic antioxidants vitamin C, vitamin E and GSH in plasma, Liver and kidney tissues. Similar results were observed in diabetic rats treated with glibenclamide. From the above results it was confirmed that AGAF has significant enzymatic and non enzymatic antioxidant activity in STZ induce diabetic rats.

Page 59: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P43 ANTI ULCER and ANALGESIC ACTIVITY OF ANNONA SQUAMOSA

LEAVES Murali Krishna ABSTRACT The anti-ulcer activity of ethanolic extract of Annona squamosa leaves was investigated on aspirin induced ulcer models and pylorus ligation in wistar rats. In both models the common parameter determined was ulcer-index. Ethanolic extract of dosage 50, 100 mg/kg p.o produced significant inhibition of gastric lesions induced by pylorus ligation and aspirin induced ulcers. The extract 50mg/kg & 100mg/kg showed significant (p<0.01) reduction in gastric volume,free acidity and ulcer index as compared to control.This present study indicate that Annona squamosa leaves extract have potential anti ulcer activity in both models. This results may further suggests that ethanolic extract was found to posses antiulcerogenic as well as ulcer healing property,which might be anti secretory activity. Key words: Annona squamosa Linn, antiulcer, aspirin, pylorus ligation.

Page 60: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P44 ANTI-PLASMODIUM FALCIPARUM ACTIVITY OF EXTRACTS FROM 10 CAMEROONIAN MEDICINAL PLANTS Abstract: Background: In the midst of transient victories by way of insecticides against mosquitoes or drugs against malaria, the most serious form of malaria, caused by Plasmodium falciparum, continues to be a major public health problem. The emergence of drug-resistant malaria parasites facilitated by fake medications or the use of single drugs has worsened the situation, thereby emphasizing the need for a continued search for potent, safe, and affordable new antimalarial treatments. In line with this need, we have investigated the antiplasmodial activity of 66 different extracts prepared from 10 different medicinal plants that are native to Cameroon. Methods: Extracts were evaluated for their capacity to inhibit the growth of the chloroquine-sensitive (Pf 3D7) and resistant (PfINDO) strains of P. falciparum using the SYBR green fluorescence method. The cytotoxicity of promising extracts against human embryonic kidney cells (HEK293T) mammalian cells was assessed by MTT assay. Results: The antiplasmodial activity (50% inhibitory concentration, IC50) of plant extracts ranged from 1.90 to >100 µg/mL against the two strains. Six extracts exhibited good activity against both Pf 3D7 and PfINDO strains, including cold water, water decoction, and ethyl acetate extracts of leaves of Drypetes principum (Müll.Arg.) Hutch. (IC503D7/INDO = 4.91/6.64 µg/mL, 5.49/5.98 µg/mL, and 6.49/7.10 µg/mL respectively), water decoction extract of leaves of Terminalia catappa L. (IC503D7/INDO = 6.41/8.10 µg/mL), and water decoction extracts of leaves and bark of Terminalia mantaly H.Perrier (IC503D7/INDO = 2.49/1.90 µg/mL and 3.70/2.80 µg/mL respectively). These promising extracts showed no cytotoxicity against HEK293T up to 200 µg/mL, giving selectivity indices (SIs) in the range of >31.20–80.32. Conclusions: While providing credence to the use of D. principum, T. catappa, and T. mantaly in the traditional treatment of malaria, the results achieved set the stage for isolation and identification of active principles and ancillary molecules that may provide us with new drugs or drug combinations to fight against drug- resistant malaria. Keywords: Plasmodium falciparum; medicinal plants; antiplasmodial activity; selectivity index

Page 61: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P45 Sujana kumari ABSTRACT

In the present work, an attempt has been made to develop controlled release tablets of oxcarbazepine by selecting of HPMC and Poly ethyleneoxide as retarding polymers. All the formulations were prepared by direct compression method. The blend of all the formulations showed god flow properties such as angle of repose, bulk density, tapped density. The prepared tablets were shown good post compression parameters and they passed all the quality control evaluation parameters as per I.P limits. Among all the formulations F10 formulation showed maximum % drug release i.e., 98.84% in 12 hours hence it is considered as optimized formulation F10 which contains Ethylcellulose N50 (20 mg) . Whereas the formulations with HPMC K15Mshowed more retarding with increasing concentration of polymer. The formulations with Poly ethyleneoxide were unable to produce the desired drug release pattern. keywords: oxcarbazepine, Poly ethylene oxide, Ethylcellulose N50, contolled release tablets

Page 62: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P46 Sujanakumari ABSTRACT The claims of Nigella Sativa Seed’s antiurolithiatic effect have not been established experimentally. The present study is designing to evaluate the invitro anti-urolithiatic activity of Hydroalcoholic and aqueous seed extracts of Nigella Sativa. Urolithiasis is a common and a major cause of morbidity worldwide. Nigella is widely utilized in traditional medicine systems. Traditional communities in India use this plant as an anti pyretic, anti-inflammatory, anti-bacterial, anti-fungal, anti-psoriasis, antioxidant, anti-cancer, anti-microbial, anti-diabetic, anti- viral, anti-parasitic, wound healing, vitiligo ,hyper sensitivity reactions etc. So far, the claims of Nigella sativa seed’s anti urolithiatic activity have not been confirmed experimentally. The present study is designed to evaluate the invitro anti- urolithiatic activity of Hydroalcoholic and aqueous seed extracts of Nigella sativa. The Seed’s of Nigella sativa were collected. The Seeds were dried and grounded to fine powder. The powder was macerated with water, ethanol and Hydroalcoholic individually for a week. Then they were filtered and concentrated by heating. The preliminary phytochemical analysis of the extracts were carried out. In the present study invitro antiurolithiatic activity was carried out. Semi permeable membranes were isolated from farm eggs. They were packed with the extracts and calcium oxalate and incubated in conical flasks containing TRIS buffer for 7-8 hours. Then the contents were taken titrated against standard potassium permanganate to estimate the amount of calcium oxalate remained. There is decrease in the amount of Calcium oxalate in both Ethanolic and Hydroalcoholic extracts indicating the antiurolithiatic activity of Nigella sativa Seed’s. In the present study ethanolic and Hydroalcoholic Seed extracts of Nigella sativa were assessed for their invitro antiurolithiatic activity i.e. stone decomposition capacity was evaluated. And the Ethanolic and Hydroalcoholic seed extracts of Nigella Sativa showed antiurolithiatic activity.

Page 63: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P47

ANTIAMOEBIC ACTIVITY OF ADENOPHYLLUM AURANTIUM (L.) STROTHER AND ITS EFFECT ON THE ACTIN CYTOSKELETON OF

ENTAMOEBA HISTOLYTICA

Vinny and Vinny Abstarct

Abstract In Mexico, the Adenophyllum aurantium (L.) Strother plant is consumed as an infusion to treat intestinal diseases such as amoebiasis, which is an endemic health problem in Mexico and other countries. However, the effect of A. aurantium on Entamoeba histolytica, the causative agent of amoebiasis, is unknown. An aerial part methanolic extract (AaMeA), a root methanolic extract (AaMeR) and a root ethyl acetate extract (AaEaR) were tested on E. histolytica trophozoites. AaMeA and AaMeR did not show antiproliferative activity; however, AaEaR exhibited an in vitro GI50 of 230 µg/ml, and it was able to inhibit the differentiation of Entamoeba invadens trophozoites into cysts. The intraperitoneal administration of AaEaR (2.5 or 5 mg) to hamsters that were infected with E. histolytica inhibited the development of amoebic liver abscesses in 48.5 or 89.0% of the animals, respectively. Adhesion to fibronectin and erythrophagocytosis were 28.7 and 37.5% inhibited by AaEaR, respectively. An ultrastructure analysis of AaEaR-treated trophozoites shows a decrease in the number of vacuoles but no apparent cell damage. Moreover, this extract affected the actin cytoskeleton structuration, and it prevented the formation of contractile rings by mechanism(s) that were independent of reactive oxygen species and RhoA activation pathways. 13C NMR data showed that the major compounds in the AaEaR extract are thiophenes. Our results suggest that AaEaR may be effective in treatments against amoebiasis, nevertheless, detailed toxicity studies on thiophenes, contained in AaEaR, are required to avoid misuse of this vegetal species. Keywords: actin, cytoskeleton, Adenophyllum aurantium, amoebiasis, Entamoeba histolytica, encystati

Page 64: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P48 TITLE: GENETIC DIVERSITY OF MYCOBACTERIUM

TUBERCULOSIS COMPLEX ISOLATED FROM SPUTUM SMEAR POSITIVE PREVIOUSLY TREATED PATIENTS WITH PULMONARY

TUBERCULOSIS. Author’s details 1. AnagoniSrikar, Research Scholar, Department of microbiology, SVIMS

University, Tirupati. 2. Dr.Venkataramana, Associate professor, Department of microbiology, SVIMS

University, Tirupati. 3. Dr.Alladimohan, Professor &amp; Head, Department of Medicine, SVIMS

University, Tirupati. 4. Dr. PVGK Sarma, Associate professor &amp; Head, Department of

Biotechnology, SVIMS University, Tirupati. 5. Dr. Abhijit Chaudhury, Professor, Department of microbiology, SVIMS

University, Tirupati. Background: M. tuberculosis drug resistance is a serious problem, especially among previously treated patients. We aimed to investigate the pattern of drug resistance and strain diversity among previously treated patients with sputum smear-positive pulmonary tuberculosis in a South Indian population. Methods: A total of 97 patients with sputum smear positivity and who had previously undergone treatment were selected. Sputum was cultured and drug susceptibility testing was done for first line tuberculosis drugs. Spoligotyping was performed on genomic DNA using standard protocol. Results: The 97 patients belonged to all age groups (10-90 years) with a mean age of 50 years; most common being in 61-70 years range(28.9%). Females were 40 (41.24%) and males 57 (58.76%). The isolates showed highest (48.4%) and lowest (6.2%) resistance to isoniazid and ethambutol respectively. Mono- resistance to isoniazid 14 (14.43%) was highest among all drugs. Significant difference (p<0.0001) was found between rifampicin and isoniazid resistant pattern. Spoligotyping results showed 34 different Spoligotyping patterns. Out of 97 isolates, 76 belonged to well defined lineages and 7 clusters; while the remaining 21 were singletons. The distribution of most common lineages were: Beijing-12 (12.37%), CAS1-Delhi- 9 (9.27%), EAI3-Ind – 20 (20.61%), EAI5- 13 (13.40%), and 7 each of Unknown and Orphan. Conclusion: Mono-resistance to isoniazid was very high among the previously treated patients. The occurrence of 12.4% Beijing strains in South India is an important finding. Spoligotyping can be very useful to delineate the lineages of MTBC in circulation in a given geographic region.

Page 65: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P49

COMPARISON OF TWO METHODS FOR MEASUREMENT OF SERUM CHLORIDE

Authors: M.KeerthiThej, K.Mamatha, Aparna R. Bitla Affiliation: Department of Biochemistry, Sri Venkateswara Institute of Medical Sciences, Tirupati Background: Discrepancies in electrolyte concentration namely in sodium and potassium using the direct and indirect ISE methods has been described. The present study was taken up to compare serum chloride values obtained using direct and indirect ISE methods. Material and methods: Serum chloride measurement was done in residual samples received in the clinical laboratory using direct ISE method and Indirect ISE method Imprecision, accuracy and comparability of the methods was studied as per the Clinical and Laboratory Standards Institute (CLSI) EP A guidelines. The American Clinical Laboratory Improvement Amendments (CLIA) guideline of acceptability of ±5% was considered as the quality goal. Results: Both the methods had acceptable imprecision and inaccuracy of less than 5%. Good agreement between the two methods was seen as assessed using Bland Altman plot and Passing Bablok regression analysis and a correlation coefficient of 0.9838. A significant difference in serum chloride levels measured in patient samples covering the entire analytical range was observed (p<0.001) with the results of serum Chloride by indirect ISE being lower compared to the direct ISE method. The difference between the two methods was below ±5% and thus met the CLIA guideline. The goal of ±5% can be crucial in the decision levels as the indirect ISE method can falsely classify patients with hyperchloremia as normochloremia. Conclusion: The findings of the present study show that serum chloride estimation using direct ISE and indirect ISE methods are comparable. However, caution is recommended in interpretation of serum chloride levels in the acute care setting.

Page 66: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P50

PROBING BINDING INTERACTION OF BOVINE SERUM ALBUMIN (BSA) WITH BIXIN AND CROCIN THROUGH SPECTROSCOPY

STUDIES AND THERMODYNAMIC PROPERTIES

P.Venkata Likhitha , V. Sai Ramya Teja Reddy , P.Vaishnavi , I. Swathi , K. Sai Rachana Manasa , Reshma Anjum , Sankari Mohan

Sri Padmavathi Mahila Viswavidhyalayam (Women’s University) Tirupati- 517501 Abstract Bixin and crocin are natural apocarotenoids consumed as food colorants and additives in food industries worldwide. For safety assessment it is essential to understand the biological interaction of food colorants. In our present study, we report the interaction of two apocarotenoids with bovine serum albumin (BSA) at physiological pH using spectroscopic techniques and in-silico tools. The binding constant and the number of binding site has been studied. The thermodynamics study showed the interaction between the bixin and crocin with BSA was examined by enthalpy, entropy, and electrostatic interaction plays an important role in these two apocarotenoids with BSA. The adverse temperature dependence in the unusual static quenching is found to be a reasonable consequence of the large activation energy requirement in the binding process, which is required to overcome the structural block and it is a direct result of the unique microstructure of the binding sites. The experimental analysis, we investigated the binding patterns using different in silico tools. A combination of molecular docking, molecular dynamics and toxicity analysis was performed and the results revealed that both the apocarotenoids had high binding affinity with a binding energy of -5.84 and -6.28 kcal/mol for bixin and crocin respectively with no toxic effects and the results are in accordance with our experimental analysis. The results directly revealed that the flexibility between the nature of the protein and the structure of bixin and crocin has a great impact in the interaction and these compounds can guardedly use as food colorants in food industries and drug delivery system Keywords:Bixin and Crocin, Bovine Serum Albumin, Fluorescence spectroscopy, Molecular docking, Molecular simulation

Page 67: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P51

ANTIFUNGAL ACTIVITY OF AQUEOUS EXTRACTS OF SOME DOMINANT ALGERIAN MEDICINAL PLANTS

Sambatturu Obulesu

Abstract This study investigated the antifungal properties of aqueous extracts obtained from indigenous plants that grow spontaneously in the Northern Sahara of Algeria. The activities of these plants in controlling two fungal species that belong to Fusarium genus were evaluated in an in vitro assay. Materials and Methods. Fresh aerial parts of four plant species (Artemisia herba alba, Cotula cinerea, Asphodelus tenuifolius, and Euphorbia guyoniana) were collected for the preparation of aqueous extracts. Two levels of dilution (10% and 20%) of the pure extracts were evaluated against Fusarium graminearum and Fusarium sporotrichioides. Results. The results of this study revealed that the A. herba alba, C. cinerea, A. tenuifolius, and E. guyoniana aqueous extracts are effective at both concentrations of 10% and 20% for the Fusarium mycelia growth inhibition. In particular, A. tenuifolius extract is effective against F. graminearum, whereas F. sporotrichioides mycelium growth is strongly affected by the E. guyoniana 20% extract. The phytochemical characterization of the compositions of the aqueous extracts has revealed that the presence of some chemical compounds (tannins, flavonoids, saponins, steroids, and alkaloids) is likely to be responsible for the antifungal activities sought. Conclusion. The antifungal properties of A. herba alba, C. cinerea, A. tenuifolius, and E. guyoniana make these plants of potential interest for the control of fungi affecting both wheat yield and safety.

Page 68: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P52

COSMOCEUTICAL VALUE OF HERBAL EXTRACTS IN NATURAL WITH NOVEL TECHNOLOGIES IN ANTI-AGING

M LAKSHMI, A DEEVAN PAUL SVU COLLEGE OF PHARMACEUTICAL SCIENCES, SV UNIVERISTY ABSTRACT: Herbal cosmetics are formulatedusing different cosmetic ingredients to form the base in which one or more herbal ingredients are used to cure various skin ailments. The name itself suggests that herbal cosmetics are natural and free from all harmful synthetic chemicals often to prove efficacy inskin sensitisations, in comparison with other beauty products the natural cosmetics are safe to use. Cosmeceuticals are cosmetics in pharmaceutical hybrid products intended to improve the health and beauty of the skin by providing a specific result, ranging from acne, control and anti-wrinkle effects to skin protection by introducing the novel technologies in anti-aging. Cosmeceuticals have medical benefits thataffect the biological functioning of skin depending upon the type of functional ingredient, by these natural cosmeceuticalsimproves the texture of skin by boosting collagen growth by eradicating harmful effects if free radicals in maintaining keratin structure in good condition for healthier skin. There are numerous natural herbs available with different uses in cosmetic preparations for skin, hair and anti-oxidant activities. The current review highlights the importance of herbal cosmetics in herbs with their advantages over synthetic counterparts Keywords: Herbal Cosmetics, Cosmeceuticals, Keratin, Skin ailments.

Page 69: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P53

PERSONALIZED AND PRECISION MEDICINE –A BUSINESS OPPORTUNITY TO GRAB WITH MINIMAL INVESTMENT AND

GREAT HEALTH ECONOMICS

Dr K.R.SUBASH, Dr K.UMAMAHESWARA RAO Affiliation-Department of Pharmacology, SVIMS, Sri Padmavathi Medical College for Women Abstract Pharmacogenomic(PG) testing services that marks an important step towards providing personalised care based on the genetic profile of patients. The genetic make-up of a person accounts for 60-70 per cent of variations in the manner and the measure to which the body responds to medication. PG testing of an individual provides important genetic information that can be used to customize therapy. The test results provide doctor vital clues about the patient’s capacity to handle certain drugs. Based on this, the physician can then choose an appropriate drug and its dosage that maximizes safety and efficacy besides bringing down costs. In patients with heart disease, around 30 per cent treated with statins, 35 per cent treated with beta- blockers and about half the patients put on tricyclic antidepressants show no response to therapy. Moreover, some patients may develop drug toxicity even at recommended doses. “The key is that PG testing provides predictive values that help doctors choose a drug within a class likely to be most effective and least hazardous in terms of side effects before patients are started on a regimen of medicines,” Experts believe that around 70 per cent of adverse drug reactions could be related to genetic profile that concern drug-handling enzymes of a patient, which can be predicted and prevented by a suitable pharmacogenomics test. WHY SVIMS ideal to set up Business Platform to Develop Pharmacogenomics

1. Department of Pharmacology at SVIMS is functioning as regional Adverse Drug Reaction with PHARM –D Interns for detection and reporting. In-house Biotechnology department with Testing facility of Genomic KITS

2. Analysis of Pharmacogenomic reporting and prediction report by In-house Pharmacologist

Page 70: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

3. Dedicated SVIMS -Community Medicine Clinic, Geriatric Clinic for Patient

Counseling with generated reports and Internal referral system to various specialty and super specialty departments

4. First of a kind in the region with minimal or negligible investment on par with international standards WHY BUSINESS STYLED-INVESTORS To Tap Health conscious affordable (geriatric) population- As they can also utilise this service during master health check-ups at hospital, so that they can have a pharmacogenomic profile report valid throughout their life. ACKNOWLEDGEMENT:Dr. Adithan C., professor of pharmacology and Director of Central Inter-Disciplinary Research Facility, MGMCRI, for his vision and mission towards pharmacogenomics.

Page 71: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P54

IRRITABLE BOWEL SYNDROME

N.G.KAMALANADHAN

Gmail : [email protected] SVUCPS ,TIRUPATI. ABSTRACT

Irritable Bowel Syndrome (IBS) is a common, chronic gastrointestinal condition defined by disturbances in bowel habits and abdominal pain, in the absence of known organic pathology. IBS reduces patients’ quality of life, and exerts a substantial, negative impact on financial resources of patients and society at large. The diagnosis of IBS is made according to a symptom based classification system, the Rome Criteria, with updated guidelines, Rome IV, recently released. Physiological and psychological variables have been identified as playing a role in the etiology and perpetuation of symptomatology, highlighting the cross-talk between the brain and the gut in patients living with IBS. Although research investigations have made gains in understanding the pathophysiology of IBS, therapeutic interventions remain symptom driven, with both pharmacological and non-pharmacological approaches in the treatment arsenal. The purpose of this review is to summarize the epidemiology, pathophysiology, diagnosis, and treatment of IBS, and to discuss implications for clinical nursing practice. Keywords: Irritable Bowel Syndrome, IBS, Rome Criteria, treatment approaches

Page 72: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P55

PROBING BINDING INTERACTION OF BOVINE SERUM ALBUMIN (BSA) WITH BIXIN AND CROCIN THROUGH SPECTROSCOPY

STUDIES AND THERMODYNAMIC PROPERTIES

P.Venkata Likhitha , V. Sai Ramya Teja Reddy , P.Vaishnavi , I. Swathi , K. Sai Rachana Manasa, Reshma Anjum , Sankari Mohan

Sri Padmavathi Mahila Viswavidhyalayam (Women’s University) Tirupati-517501 Abstract Bixin and crocin are natural apocarotenoids consumed as food colorants and additives in foodindustries worldwide. For safety assessment it is essential to understand the biological interaction of food colorants. In our present study, we report the interaction of two apocarotenoids with bovine serum albumin (BSA) at physiological pH using spectroscopic techniques and in-silico tools. The binding constant and the number of binding site has been studied. The thermodynamics study showed the interaction between the bixin and crocin with BSA was examined by enthalpy, entropy, and electrostatic interaction plays an important role in these two apocarotenoids with BSA. The adverse temperature dependence in the unusual static quenching is found to be a reasonable consequence of the large activation energy requirement in the binding process, which is required to overcome the structural block and it is a direct result of the unique microstructure of the binding sites. The experimental analysis, we investigated the binding patterns using different in silico tools. A combination of molecular docking, molecular dynamics and toxicity analysis was performed and the results revealed that both the apocarotenoids had high binding affinity with a binding energy of -5.84 and -6.28 kcal/mol for bixin and crocin respectively with no toxic effects and the results are in accordance with our experimental analysis. The results directly revealed that the flexibility between the nature of the protein and the structure of bixin and crocin has a great impact in the interaction and these compounds can guardedly use as food colorants in food industries and drug delivery system Keywords:Bixin and Crocin, Bovine Serum Albumin, Fluorescence spectroscopy, Molecular docking, Molecular simulation

Page 73: ORAL PRESENTATIONsvimstpt.ap.nic.in/symposium_files/finalsymp.pdf · PRESENTATION . ROLE OF MEDITATION IN CARDIOVASCULAR DISEASES . Dr.Vijaya Chandra Reddy Konda,* Associate Professor

P56 GOOSENECK FLEXIBLE BRAIN RETRACTOR – UTILITY OF

GOOSENECK TUBING IN NEUROSURGERY. Ramesh chandra vv, Prasad bcm, Goutham hanu* Department of

Neurosurgery,SVIMS . Abstract Brain retractor is an indispensable instrument in Neurosurgery instrument set. The initial hand held brain retractors are in use since 1885. Flexible table mounted brain retractors are in use since 1977 (Leyla retractor). There has been no progress in the retractor systems after 1977 and all Neurosurgeons use this retractor. Gooseneck tubing is an engineering marvel and has many applications (Reading lamp, Microphone, Tablet/phone holder etc) .We present a brain retractor using this technology. Methods and Results: The traditional flexible brain retractor (Leyla) consists of three principal parts: a fixation base, a flexible arm or arms, and a blade holder.Leyla mechanical arm is composed by multiple portions inserted similar to a tulip with an adjustable stainless steel wire inside. The arm needs to be adjusted frequently by adjusting the tension in the wire this adjustment is subjective and excessive tension may lead to pressure/injury to the brain. The mobility of this arm is restricted and does not have a 360 degree exposure. The retractor devised by us has the same components as a traditional retractor except the flex arm. The flex arm is replaced by an ultra- light duty Gooseneck tubing. This allows a very passive and light retraction on the brain. The flex arm has a greater degree of movement and can be used to retract all regions of the brain with out changing the fixation point. This retractor is cheaper than the traditional retractor. The retractor proved very useful in most of the cranial surgeries and no complications were noted during its use. Conclusion: Gooseneck flexible Brain Retractor is a safe and cheaper alternative to the present available brain retractor system. The retraction provided is passive as it does not involve active tightening of the flex arm. The efficacy and supremacy over traditional retractor systems need to be confirmed by further studies.