Ontogenetic peculiarities and regenerative potentials of brain

vascular plexuses in case of xenogenic cerebrospinal fluid

injection.

Crimea State Medical University named after S.I. Georgievsky

Human normal anatomy department

Prepared by:I.H. Gasanova

Scientific advisor:prof. V.S. Pykaluk, MD

The GoalThe Goal::

is to study special morpho-functional characteristics of is to study special morpho-functional characteristics of brain vascular plexuses in case of xenogenic cerebro-brain vascular plexuses in case of xenogenic cerebro-spinal fluid injection spinal fluid injection

Research materialsResearch materials::vascular plexuses of certain age Wistar vascular plexuses of certain age Wistar

white ratswhite rats

Research methods:

Organometric indicatorsOrganometric indicatorsHistological examination of vascular plexusesHistological examination of vascular plexuses

Histochemical examination of choroid plexusesHistochemical examination of choroid plexusesStatistical data evaluationStatistical data evaluation

juvenile control

experiment

7 days

7 days

6 rats

6 rats

adult control

experiment

7,30,90 days

7,30,90 days

6 rats/group

7 rats/group

presenile control

experiment

7,30,90 days

7,30,90 days

6 rats/group

7 rats/group

age group slaughtering date

number of animals

Table of experiment

0.9% NaCl

CSF

control

experiment

Ventricular system of the brain

Lateral ventricles and third ventricle, frontal section

Lateral brain ventricle vascular plexus, plexus choroideus ventriculi lateralis. Well vascularized choroid structure penetrates to the brain ventricle through vascular fissure. Anteriorly it gets to interventricular foramen, posteriorly it protracts to inferior horn. Figure Б.

Vascular plexuses physiology: - vascular plexuses and cerebro-spinal fluid:

CSF secretion theory CSF filtration theory

CSF resorption theoryCSF dialysis theory

- vascular plexuses and brain-blood barrier :

hemato-liquor barrier hemato-cerebral barrier liquoro-cerebral barrier

Organometric evaluation of a brain

–brain mass–hemisphere length(1) –hemisphere width(2) –hemisphere heigth(3)

2 1

3

Brain mass indicatorsBrain mass indicators

((pp<0.05).<0.05).

- Cephalization index: Е2/РЕ- brain mass, Р- body mass

-percentage ratio of brain mass to body mass 0,9-1% (at adult rats)

Cephalization index indicators

((pp<0.05).<0.05).

Brain hemispheres length

Brain hemispheres width

Organometric characteristics of brain hemispheres

((pp<0.05).<0.05).

Hematoxylin-eosin staining

Brain vascular plexus microscopy

Schiff reaction staining to determine GAG type(acidic GAG in intercellular space and neutral GAG inside arteries)

ConclusionConclusion::• The function of brain vascular plexuses is complicated a lot. This The function of brain vascular plexuses is complicated a lot. This

function also changes much during the life period or due to function also changes much during the life period or due to different functional statuses of organismdifferent functional statuses of organism. . Therefore it’s hard to Therefore it’s hard to define the single common function for choroid plexuses. The most define the single common function for choroid plexuses. The most probable assumption is that it does the combinatorial function probable assumption is that it does the combinatorial function ((combinatorial theorycombinatorial theory))

• With the increase of age brain mass in rats increases also and rises With the increase of age brain mass in rats increases also and rises 0,9-1% 0,9-1% of brain mass in adult animalsof brain mass in adult animals. . Conversely, cephalisation Conversely, cephalisation index decreases step by step in an age and the presenile animals index decreases step by step in an age and the presenile animals have a low level of ithave a low level of it. . Initially grows the brain length, later in adult Initially grows the brain length, later in adult rats starts to grow fast in adult. As a result maximum length is rats starts to grow fast in adult. As a result maximum length is defined in adult rats and maximum width is in a presenile age.defined in adult rats and maximum width is in a presenile age.

• Brain vascular plexus histological examination shows embrionic Brain vascular plexus histological examination shows embrionic epithelium in plexuses of newborn and juvenile rats. With the epithelium in plexuses of newborn and juvenile rats. With the increase of age also grows a number of cells and collagenous and increase of age also grows a number of cells and collagenous and elastinic fibers. Epithelium cells decrease in size in an presenile elastinic fibers. Epithelium cells decrease in size in an presenile animals.animals.

• Brain choroid plexus histochemical examination shows acidic GAG Brain choroid plexus histochemical examination shows acidic GAG in intercellular space and neutral GAG inside arteries.in intercellular space and neutral GAG inside arteries.

Thanks for your

attention!