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Ahmed Morad Asaad M.B.B.S, M.Sc., M.D.

Professor of Microbiology & Immunology

Coordinator of Microbiology Department

Scientific Deputy of Prince Meshaal Bin Abdul-Aziz

Chair for Endemic Diseases

College of Medicine, Najran University

Najran

Saudi Arabia

P.O Box 1988

Phone: +966530584013 - +966725448516

E-mail: ahmedmoradasaad@hotmail – amasad@nu.edu.sa

Field of Interest: Bacterial infections

Molecular antimicrobial drug resistance

Mycobacteriology

Viral infections

Fungal infections

Molecular biology techniques

Immunological techniques

Membership of Societies and Associates: -Saudi Commission for Health Specialties: Consultant Microbiologist Professional

Registration

-Member of Egyptian Medical Microbiology Society (EMMS).

- Editorial Board member in Clinical Microbiology Open Access Journal – OMICS

- Editorial Board member in Global Scientific Inc Publishing Journals

- Editorial Board member in HSOA Journal of Infectious and non Infectious

Diseases – Herald Scholarly Open Access

- Reviewer in many international and national (Egyptian and Saudi) peer-reviewed

Journals

-Head of Infection Control Committee, Najran University Hospital, Najran, Saudi

Arabia

Research Under

Progress:

Research Under Progress:

Mohamed AlAyed, Asaad AM, Mohamed Ansar Qureshi, Mohamed

Ameen. Viral etiology of respiratory infections in children in Najran, Saudi

Arabia, using the multiplex RT-PCR. Saudi Medical Journal (In Press).

Jobran Miree Alqahtani, Asaad AM, Essam Mohamed Ahmed, Mohamed

Ansar Qureshi. Assessment of bacteriological quality of drinking water in

Najran, southwestern Saudi Arabia. Clean SoilAir & Water Journal (In

Press).

Elabd FM, Zayed MS, Asaad AM, Alseeri S, Qureshi MA and Mussa HA:

Molecular characterization of oxacillinases among nosocomial

Acinetobacter baumannii isolated from a Saudi hospital. J. Infec. Pub. Health

(In Press).

Asaad AM, Metawee ME, Zayed MS and Qureshi MA. Distribution and

implications of Hepatitis B Virus (HBV) genotypes in Saudi patients with

different HBV clinical presentations in Najran Region. (Under progress)

Publications:

Alqahtani JM, Abu-Eshy SA, Mahfouz AA, El-Mekki AA and Asaad AM:

Seroprevalence of hepatitis B and C virus infections among health

students and health care workers in the Najran region, southwestern

Saudi Arabia: The need for national guidelines for health students. BMC

Public Health 2014; 14: 577-583.

Alqahtani JM and Asaad AM: Anti-tuberculous drugs susceptibility

testing methods: Current knowledge and future challenges.

Mycobacterial Diseases Journal 2014, 4: 140-146. doi:10.4172/2161-

1068.1000140.

Asaad AM, Zaid MS, Qureshi MA: Emergence of unusual nonfermenting

gram negative nosocomial pathogens in a Saudi hospital. Japanese

Journal of Infectious Diseases 2013; 66: 507-511.

Asaad AM, Qureshi MA: Increased vancomycin minimum inhibitory

concentrations of methicillin-resistant Staphylococcus aureus nosocomial

isolates from southwestern Saudi Arabia. American Journal of

Epidemiology and Infectious Diseases 2013; 1: 59-62.

Asaad AM, Jobran Miree Alqahtani: Primary anti-tuberculous drugs

resistance of pulmonary tuberculosis in Southwestern Saudi Arabia. 3rd

International Conference and Exhibition, 2013 on Pharmaceutics &

Novel Drug Delivery Systems, Chicago/Northbrook, USA.

Mahmoud MM, Asaad AM, Qureshi AM: Hand rubbing and scrubbing in

relation to microbial count among surgical team members in a Saudi

Hospital. Life Science Journal 2013; 10: 198-205.

Mahmoud MM, Asaad AM: SURGICAL ASEPSIS PRACTICES AMONG

OR STAFF IN KING KHALID HOSPITAL, NAJRAN. International

Journal of Current Research 2013; 5: 3461-3473.

Zaid MS, Asaad AM, Alhayek AA, Qureshi MA: Etiology of acute

gastroenteritis in children in Najran Saudi Arabia. Journal of Health

Specialities 2013; 1: 28-33.

Asaad AM, Alqahtani JM: Primary antituberculous drugs resistance of

Pulmonary tuberculosis in southwestern Saudi Arabia. Journal of

Infection and Public Health 2012; 5: 281-285.

Asaad AM, Alqahtani JM: Serological and molecular diagnosis of human

brucellosis in Najran, Saudi Arabia. Journal of Infection and Public

Health 2012; 5: 189-194.

Asaad AM, Al Yousef S, Al gharawi A: Metallo-β-Lactamase producing

Pseudomonas aeruginosa clinical isolates from a Saudi Military Hospital. Egy.

J. Med. Microbiol. 2009; Vol. 18, No. 3: 91-98.

Howyda MI, Asaad AM and Ahmed Amer: Antifungal susceptibility

patterns of Dermatophytes clinical isolates from dermatophytosis patients

before and after therapy. Egy. J. Med. Microbiol. 2010; Vol. 19, No. 1: 41-

46.

Ahmed AS, Asaad AM, Amal HA and Azza OA: Phenotypic and genotypic

patterns of aminoglycosides-resistant Staphylococcus aureus clinical isolates.

Egy. J. Med. Microbiol. 2010; Vol. 19, No. 2: 1-8.

Ahmed SS, Abeer MN, Asaad AM and Ayman AA: Clinical and

microbiological features of nosocomial infections caused by Acinetobacter

species. Egy. J. Med. Microbiol. 2010, Vol. 19, No. 2: 121-130.

Abeer M. Nafee and Asaad AM: Antimicrobial resistance trends and

distribution of virulence genes among Shigella isolates from patients with

acute diarrhea. J. Trop. Med. 2010; Vol. 11, No. 4.

Asaad AM, Jehan A El-Shennawy, Howyda M Ebrahim and Hala M Morsi:

Staphylococcus aureus isolates from patients with atopic dermatitis: Clinical,

bacteriological and molecular characters. Egy. J. Med. Microbiol. 2010;

Vol. 19, No. 3: 87-96.

Jehan A El-Shennawy and Asaad AM: Comparative study of different

methods to detect primary and non primary cytomegaloviral infection in

pregnant women. Egy. J. Med. Microbiol. 2010; Vol. 19, No. 4: 93-102.

Neveen H.A, Hanan MT and Asaad AM: Control of Listeria

monocytogenes and Staphylococcus aureus isolated from chicken meat and

chicken products by dipping in some organic acid solutions. Bulletin of

Animal Health and production in Africa 2008; Vol. 56, No. 4: 271-

279.

Asaad AM, Al Yousef S: Surveillance of antimicrobial resistance in a

Military Hospital, Saudi Arabia using WHONET 5 program. Egy. J. Med.

Microbiol. 2007; Vol. 16, No. 1: 79-94.

Asaad AM. WHONET: What is it? NAAFH CPE Bulletin, 2007; vol. 8,

No. 5: 5.

Asaad AM. Lecture Notes on Microbiology. Nursing Diploma Program

Book, 2005. Northern Area Armed Forces Hospital, King Khalid Military

City, Saudi Arabia.

Asaad AM, Al-Bialy AA and Awad-Alla MG. Bacteriological and molecular

aspects of Staphylococcus aureus clinical isolates carrying genes for

exfoliative toxins and Panton-Valentine leukocidin. Egy. J. Med. Microbiol.

2004; Vol. 11, No. 1.

Asaad AM, Amer A and Abd El-Dayem WA. Performance of enzyme

immunoassay and PCR for rapid diagnosis of Helicobacter pylori infection.

Egy. J. Med. Microbiol. 2003; Vol. 12, No. 2.

Asaad AM, El-Behedy EM and Amer A. Diagnosis of Shigella infection in

children with acute diarrhea by conventional and molecular methods. Egy.

J. Med. Microbiol. 2003; Vol. 12, No. 1.

Tema MA, Asaad AM, Mamoon M. Use of PCR for diagnosis and follow

up of leprosy patients during multi-drug therapy. Egy. J. Med. Microbiol.

2003; Vol. 9, No. 2.

Elshamy EA and Asaad AM. Some pro-inflammatory and anti-

inflammatory cytokines in cirrhotic ascetic patients with and without

spontaneous bacterial peritonitis. Egy. J. Med. Microbiol. 2003; Vol. 9, No.

3.

Hasanen OM, Asaad AM and Allam A. Prevalence of TT virus infection in

Egyptian children and adults and in patients with liver disease. Egyp. J.

Intern Med 2002; vol. 14, No.4.

Abd El-Dayem WA, Sherben AS, Elshami EA and Asaad AM.

Antineutrophil cytoplasmic antibodies types and titers in ulcerative colitis

patients. Relationship with disease variables. Zag Univ Med J 2002, vol. 8,

No. 7.

Asaad AM, Abo-Taleb AF and Mahfouz T. Susceptibility testing of

Mycobacterium tuberculosis in clinical isolates by three different methods.

Egy. J. Med. Microbiol. 2001; Vol. 10, No. 4.

Our ongoing research project

Distribution and implications of Hepatitis B Virus

(HBV) genotypes in Saudi patients with different

HBV clinical presentations in Najran Region

Hepatitis B virus (HBV) infection remains a serious health

problem. According to the homogeneity of virus sequences, at

least 10 HBV genotypes (A to J) have been defined throughout

the world with a characteristic geographical distribution. Several

HBV genotyping methods are in widespread use, such as direct

sequencing, restriction fragment length polymorphism analysis,

reverse hybridization, and enzyme-linked immunosorbent assays.

Within the last few years, a well standardized multiplex nested

PCR protocol was developed for simultaneous detection of HBV

load and identification of HBV genotypes A to G.

The knowledge regarding clinical significance of HBV

genotypes in terms of clinical outcomes and therapeutic

response to antiviral therapy in patients with HBV related

severe liver conditions is still very limited and results from

different research groups were discordant. In Saudi Arabia,

although HBV is hyperendemic, little is known about the

prevalence and distribution of HBV genotypes.

Furthermore, the association between the distinct

genotypes and the severity of liver disease in the country

remains unreported. To our knowledge, this study will be

the first to determine the prevalence and distribution of

HBV genotypes in HBV patients using multiplex nested PCR

protocol and investigate the implications of HBV genotypes

in HBV patients with different clinical presentation.

This study aims to determine the prevalence and

distribution of HBV genotypes in HBV patients and

investigate the implications of HBV genotypes in HBV

patients with different clinical presentation.

Research Methodology:

Design

The study will be a cross-sectional study on a

representative sample of HBV patients attending Najran

University Hospital, Maternity and Children Hospital and

King Khalid Hospital, Najran, Southwestern Saudi Arabia.

Clinical assessment of cases

The study will include 100 Saudi patients with HBV

infection. They will be subjected to:

Clinical assessment

Liver function tests (billirubin, AST and ALT)

HBV markers (HBs Ag, HBe Ag, Anti-HBs Ab, Anti-HBe Ab)

α-fetoprotein

The patients will be classified into 3 groups on the basis of

clinical and laboratory findings:

Group 1 (acute HBV)

Group 2 (chronic HBV)

Group 3 (HBV patients with hepatocellular carcinoma)

Blood samples will be collected from all patients for

molecular analysis.

Processing and handling of clinical specimens

All samples will be subjected for DNA extraction by a

commercial kit from reputed manufacturers. Samples will be

frozen at -20⁰C for further analysis.

Multiplex nested PCR

DNA amplification by multiplex nested PCR using well

defined sequence primers in two tubes:

The first tube for detecting HBV genotypes A, B or C

The second tube for detecting HBV genotypes D, E, F or G)

Clinical Microbiology-Open Access

Bacteriology & Parasitology Clinical Microbiology: Open

Access

Virology & Antiviral Research

Virology & Mycology

2nd International Congress on

Bacteriology and Infectious Diseases

3rd International Conference on Clinical

Microbiology & Microbial Genomics

Clinical Microbiology-Open Access

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