Oilseed TAG yields from plant leaves James Petrie, CSIRO

“Producing biomass with 10% oil on a

dry weight basis could have a major

positive impact… Is such engineering of

energy crops feasible?” 1

1. Quote from Ohlrogge & Chapman, Biochemist. 33(2) (2011).

Oilseed TAG yields from plant leaves James Petrie, CSIRO

Global mineral oil vs. plant oil utilisation

Fuels

(4.3 Gt)

Petrochem.

(400 Mt)

Mineral Oils (4.7Gt) Carlsson et al. (2011) Eur J Lipid Sci Technol 113: 812-831

Oilseed TAG yields from plant leaves | James Petrie

Aviation fuel

(250 Mt)

Global mineral oil vs. plant oil utilisation

Fuels

(4.3 Gt)

Petrochem.

(400 Mt)

Mineral Oils (4.7Gt)

Food oils

(120 Mt)

Oleochemicals

(20 Mt)

Vegetable Oils (140Mt)

Carlsson et al. (2011) Eur J Lipid Sci Technol 113: 812-831

Oilseed TAG yields from plant leaves | James Petrie

Aviation fuel

(250 Mt)

Three P’s of oil accumulation

Oilseed TAG yields from plant leaves | James Petrie

Three P’s of oil accumulation

Oilseed TAG yields from plant leaves | James Petrie

Surprising positive synergies found Transcription factor + assembly enzyme can be synergistic

0

0.5

1

1.5

2

2.5

3

Control WRI1 DGAT1 WRI1+DGAT1

TAG

(%

DW

)

Oilseed TAG yields from plant leaves | James Petrie

Surprising positive synergies found Transcription factor + assembly enzyme can be synergistic

0

0.5

1

1.5

2

2.5

3

Control WRI1 DGAT1 WRI1+DGAT1

TAG

(%

DW

)

Oilseed TAG yields from plant leaves | James Petrie

Increasing leaf oil content Multiple target approach: the Three P’s

Oilseed TAG yields from plant leaves | James Petrie

Increasing leaf oil content DNA construct design for efficient gene expression in tobacco

Oilseed TAG yields from plant leaves | James Petrie

Increasing leaf oil content DNA construct design for efficient gene expression in tobacco

Broad leaf biomass crop: tobacco

Oilseed TAG yields from plant leaves | James Petrie

High leaf oil tobacco Surprising spike in oil production late in lifecycle

17.1% TAG 23.4% TFA

69 days 104 days 139 days

Oilseed TAG yields from plant leaves | James Petrie

High leaf oil tobacco MALDI-Orbitrap imaging clearly shows TAG accumulation

University of North Texas

Oilseed TAG yields from plant leaves | James Petrie

High leaf oil tobacco 3D confocal images

University of North Texas

Oilseed TAG yields from plant leaves | James Petrie

High leaf oil tobacco 3D confocal images

University of North Texas

Oilseed TAG yields from plant leaves | James Petrie

High leaf oil tobacco Plants do not have significant negative phenotype

Oilseed TAG yields from plant leaves | James Petrie

High leaf oil tobacco Fatty acid profile changes significantly

Oilseed TAG yields from plant leaves | James Petrie

High leaf oil tobacco Oil can be extracted from leaf

Oilseed TAG yields from plant leaves | James Petrie

• High biomass crops > 20 T/Ha

• 15% TAG = 3 T/Ha

Summary 1. Three P’s of oil accumulation

Oilseed TAG yields from plant leaves | James Petrie

Summary 1. Three P’s of oil accumulation

2. 17% TAG in tobacco leaf

Oilseed TAG yields from plant leaves | James Petrie

Summary 1. Three P’s of oil accumulation

2. 17% TAG in tobacco leaf

3. The oil can be extracted

Oilseed TAG yields from plant leaves | James Petrie

Thank you CSIRO James Petrie

t +61 2 6426 4985 e james.petrie@csiro.au

CSIRO National Research Flagship Surinder P. Singh Thomas Vanhercke Uday K. Divi Anna El Tahchy Phil J. Larkin Qing Liu Maged P. Mansour

Peter D. Nichols Jean-Philippe Ral Pushkar Shrestha Xue-Rong Zhou

Rothamsted Research Frederic Beaudoin

University of North Texas Kent D. Chapman Patrick J. Horn Christopher N. James