Oil Zapper

TERI (The Energy and Resources Institute) Darbari Seth Block, IHC Complex, Lodhi Road, New Delhi - 110 003, INDIA, Tel. (+91 11) 2468 2100 and 41504900, Fax (+91 11) 2468 2144 and 2468 2145, E-mail [email protected]

Copyright © 2010 TERI

mailto:[email protected]

Bioremediation An Eco-Friendly Solution For Restoration of oil lakes

and soil remediation


What is Bioremediation ? Bioremediation : Naturally occurring microorganisms for remediation

of harmful substances to nontoxic compounds.

• Used for clean up of oil spills, oily sludge, oil contaminated

site, oil contaminated water , any other type of contamination

like pesticide contamination etc.

• End product CO2, water & cell biomass

• More Economical & Environment-friendly compared to other

conventional methods, like incineration etc.


TERI’s experience on bioremediation •Research in Bioremediation was initiated by TERI in 1991 after the gulf war.

•TERI’s bioremediation process is a customized approach where the type of contamination along with geo-climatic conditions are considered.


TERI’s Customized bioremediation process Development of indigenous microbial consortia capable of petroleum hydrocarbon degradation based on •Assessment of level and types of contamination •Assessment of Age and history of contamination •Assessment of geo-climatic conditions


Customized Microbial Products of TERI for bioremdiation


TERI’s experience on Bioremediation

Till date TERI has successfully carried out around 200,000 Metric Tonnes of petroleum wastes. Currently 60,000 MT of petroleum wastes is under bioremediation.

Types of petroleum waste bioremediated by TERI

• Dry oil contamination

• Oily sludge

• Emulsified oily sludge

• Wet oil contamination

• Oil contaminated piles and soil

• Marine oil contamination

• Oil based mud

• Oil contaminated drill cuttings

• Oil trench related contamination


Bioremediation of dry oil contamination at BPCL Refinery, Mumbai, India

Before bioremediation

After bioremediation


Bioremediation of wet oil contamination at Duliajan (Assam), India


After bioremediation


Bioremediation of oil trench at Oil India Ltd. India


After bioremediation by Oilzapper



Bioremediation of oil lake at ONGC, Assam, India

Before Bioremediation

After Bioremediation by oilzapper


Bioremediation of a oily sludge dumping site Mathura refinery, India

Before Bioremediation

After Bioremediation


Restoration of oil contaminated soil at Oil India Ltd. India

Before

After Application of Oilzapper


Bioremediation of oil lake at Digboi, India


Restoration of oil contaminate site through Bioremediation process


Restoration of oil lake

Site before bioremediation Site after bioremediation Natural vegetation after bioremediation

Aquatic birds in site filled with water

Fish culturing in site filled with rain water after bioremediation


Bioremediation of dry oil contaminated sites at Gujrat, India


Restoration of waste lands through bioremediation technology


Restoration of oil lake

Bioremediation Site at BGEPIL , Bhavanagar, Gujarat, India (Before bioremediation)

Bioremediation Site at BGEPIL , Bhavanagar, Gujarat, India (After bioremediation)

Plants grown on bioremediated SOBM waste at bioremediation site of BGEPIL, Bhavanager, INDIA

Crops grown on bioremediated SOBM waste at bioremediation site of BGEPIL, Bhavanager, INDIA


Green belt development with the treated SOBM waste at BGEPIL, Bhavanagar, India


Bioremediation of oil contaminated Piles at ONGC Mehsana, Gujarat, India

At zero day

After 45 days of application of Oilzapper


Clientele of bioremediation technology since 1991

• Indian Oil Corporation Ltd. (IOCL), India

• Hindustan Petroleum Corporation Ltd. (HPCL), India

• Bharat Petroleum Corporation Ltd. (BPCL), India

• Oil and Natural Gas Corporation Ltd. (ONGC), India

• Oil India Ltd (OIL), Assam, India

• Cairn Energy Pty. Ltd., India

• BG Exploration & Production India Ltd., India

• Reliance Energy Ltd., India

• Mangalore Refinery & Petrochemicals Ltd. (MRPL), India

• Kuwait Oil Company (KOC), Kuwait (Demonstrated)

• Abu Dhabi National Oil Company (ADNOC), Abu Dhabi.


List of Publications in scientific Journals • S Krishnan, P M Sarma and Lal B. 2006. Comparative analysis of phenotypic and genotypic

characteristics of two desulphurizing bacterial strains, Mycobacterium phlei SM120-1 and

Mycobacterium phlei GTIS10. Letters in Applied Microbiology. 42 : 483-489

• Prasad G S, Mayilraj S, Sood N, Singh V, Biswas K, and Lal B. 2005. Candida digboiensis

sp.nov. a novel anamorphic yeast species from an acidic tar sludge-contaminated oil field.

International Journal of Systematic and Evolutionary Microbiology 55: 633–638.

• Mishra S, Sarma P M, and Lal B. 2004. Crude oil degradation efficiency of a recombinant lux

tagged Acinetobacter baumannii strain and its survival in crude oil contaminated soil microcosm.

FEMS Microbiology Letters. 235: 323-331.

•

• Sarma P M, Bhattacharya D, Krishnan S, and Lal B. 2004. Assessment of intraspecies diversity

among strains of Acinetobacter baumannii isolated from sites contaminated with petroleum

hydrocarbons. Canadian journal of Microbiology. 50: 405-414.

• Sarma P M, Bhattacharya D, Krishnan S, and Lal B. 2004. Degradation of polycyclic aromatic

hydrocarbon by a newly discovered enteric bacterium, Leclercia adecarboxylata. Applied and

Environmental Microbiology. 70: 3163-3166.

• Bhattacharya D, Sarma P M, Krishnan S, Mishra S, and Lal B. 2003. Evaluation of the Genetic

Diversity among the Strains of Pseudomonas citronellolis isolated from oily Sludge Contaminated

Sites. Applied and Environmental Microbiology. 60: 1435-1441.

• Mishra S., Jyot J., Kuhad, R., and Lal B., 2001. Evaluation of inoculum addition to stimulate in

situ bioremediation of oily sludge contaminated soil, Applied and Environmental Microbiology,

67:1675-1682.

• Mishra S., Jyot J., Kuhad, R., and Lal B., 2001. In situ bioremediation potential of an oily sludge

degrading bacterial consortium. Current Microbiology 43: 328-335


Patents

• A process for enhanced recovery of crude oil from oil wells using novel

microbial consortium. Patent No. PCT/IN 2004/000206. (International

Patent)

• Bioremediation of acidic sludge. Patent No. 385. MUM/2004.

• A process for enhanced recovery of crude oil from oil wells using novel

multi-microbial strain. Patent No 892/DEL/2003.

• Microbial nutrients for biodegradation of oil refinery waste and process for

bioremediation of oily sludge and crude oil spill sites. Patent No

168/DL/2000.

• A method for preventing wax deposition in an Oil Well - bore using

microbial means. (Series no. CBR 1955, Application number

1437/DEL/2007).


Awards won (Total 10 Awards : International & National)

BPPDC Award 2002 Burhani Foundation 2002 NRDC Award 2005

AIBA Award 2001 BPPDC Award 2008 National Bioscience 2005


Awards won (Total 10 Awards : International & National)


Bioremediation of Oil Contaminated Site by KT-Oilzapper at an Oil Field of Kuwait Oil Company

From Oil

To Soil

The Energy and Resources Institute Delham General Trading & Contracting Co


Development of customized bioremediation process for Kuwait

• Oil contaminated soil samples were collected from Kuwait oil field (near

GC 1 and GC2 in 2001) for development of indigenous microbes for

customized bioremediation process.

• Total 50 bacterial strains were isolated from the above soil that could

degrade Tar crete sludge, kuwait crude oil, and oily sludge under local

geo-climatic conditions.

• Biodegradation efficiency of all 50 strains were studied at temperature up

to 60ºC and salinity up to 8% in TERI’s lab at New Delhi.

• Out of 50 strains finally four bacterial strains were selected which could

degrade all the fractions of TPHs at temperature up to 60ºC and 8 %

salinity

• A bacterial consortium named KT oilzapper was developed by

assembling the four selected strains


Degradation of alkane fraction of Kuwait crude oil at 55°C


Biodegradation of alkane compound By A.baumannii


Mineralization of alkane compound by A.baumannii


Mechanism of Action

Click to play video

C:/Documents and Settings/Administrator/Local Settings/Temp/bacteria.mp4


Degradation of aromatic fraction of Kuwait crude oil in 7 days at 55°C


Biodegradation of PAH compound by A.odorans


Mineralization of aromatic hydrocarbon compound by A.odorans


Biodegradation of sulphur containing compound of crude oil


Degradation of Asphaltene


KT-Oilzapper


Laboratory Facility


Pilot demonstration of bioremediation of dry oil contamination at KOC

• Period of pilot study: October 2004 to December 2004

• Site dimensions (70 m x 70 m)

• Application of KT-Oilzapper and nutrient recipe (patented)

• Tilling and monitoring of the bioremediation site at regular intervals


Selection of oil contaminated site



Bioremediation site contaminated with liquid oil and water

Liquid tar-crete at bioremediation site at KOC oil field



Liquid oily tar-crete at bioremediation site at KOC oil field

Liquid oil (tar-crete) and water at bioremediation site



Depth of contamination at bioremediation

Deep contamination (1.5 metre) in soil at bioremediation site


Oilzapper before application


Application of KT-Oilzapper at bioremediation site of KOC oil field


Preparation of nutrient mixture for application at bioremediation site


Spraying of nutrients at bioremediation site


Tilling of bioremediation site by mechanical tiller


Tilling of bioremediation site by mechanical tiller


Total petroleum hydrocarbons in soil at bioremediation site


Results

Biodegradation of alkane and aromatic fraction in Block A


Soil analysis report from ICAR, New Delhi, Government of India





From waste OIL To fertile SOIL


From waste OIL To fertile SOIL




Bioremediation core Team Members


Thank You!

TERI (The Energy and Resources Institute) Darbari Seth Block, IHC Complex, Lodhi Road, New Delhi - 110 003, INDIA, Tel. (+91 11) 2468 2100 and 41504900, Fax (+91 11) 2468 2144 and 2468 2145, E-mail [email protected]

mailto:[email protected]