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Standardized procedures for microbiological examinations
of food productsn acc. EN-ISO Standards n
www.scharlab.com [email protected]
NEW Certificates
New certificate including picture of typical growth.
Additives and supplements in vial
New sterilized presentation: fast, easy and less risk of contamination. Click, shake and dispense.
Prepared culture media
Selection of the most common media in different presentations.
90 mm Petri plates. Available also with single and double “flow pack bag” and gamma irradiated for use in clean room.55 mm filtration plates. Prepared plates of solid medium for the membrane filtration technique. Packed in blister. Rodac plates for surfaces control. Packaging into blisters inside clean room to avoid contamination and dehydration.Agars in flasks. Long shelf life in different volumes for remelting.Broths in flasks. Enrichment media and diluents. Sterility control.Agars in tubes for remelting. For mass inoculation technique.Broths in tubes. Differents volumes depending on working protocol.
Scharlau microbiologyHistory
Scharlau Chemie S.A. has been manufacturing laboratory chemicals since 1949. In 1979 ADSA-MICRO started the manufacture of dehy-drated media. This company was acquired by Scharlau Chemie S.A. in 1992 and the brand changed to Scharlau.A new facility for the manufacture of dehydrated media was set up in 2002, which allowed us to increase capacity and to further improve the quality of our media.ISO 9001:2000 quality assurance
was put in place since 1995.ISO 14001:1996 for environmental care was obtained in 2002.In 2002 Scharlau Chemie S.A. started offering also a wide range of prepared media and supplements.
Microbiological culture mediaScharlau manufactures two separate lines of culture media, dehydrated and prepared media; also stains in solution, reagents, su-pplements and rapid confirmation tests (see more information at the end of this leaflet). Please ask for the specific catalogues and brochures.
Dehydrated culture media
Scharlau Chemie S.A. manufactures dehydrated culture media for microbiology. •Available in 100 g and 500 g bottles•Each bottle comes in an individual bag to avoid dust and a COA (certificate of analysis) is included with each bottle•Description in 5 languages•Tamper-proof cap•No inside aluminium foil•Perfectly tight-closing cap insures long shelf life
NEW Packaging
PAC-O-VAC® is the novel airtight packing from Scharlau that provides an extraordinary protection to microbiology culture media. Vacuum, followed by replacement of residual air with nitrogen, guarantees that our products are protected from contamination by moisture, dust particles or microorganisms.This assures optimum storage conditions and
prevent the products from altering their organoleptic or physical properties.
1 2
Index• Scharlau microbiology• Staphylococcus aureus and other species• Escherichia coli• Enterobacteriaceae• Coliforms• Listeria• Microorganisms• Bacillus cereus• Yeasts and moulds• Clostridium perfringens• Salmonella spp. • Food products determinations• Technical documentation
Tel. +34 93 745 64 00 Fax +34 93 715 27 65
PresentationIn this brochure we have put toge-ther the latest ISO directives that have been published for the analysis of food.
You will be able to find other media used in microbiological food analy-sis in our latest Handbook edition no. 8.
Microbiological quality control invol-ves the measurement of the general contamination level (total amount of microorganisms) as well as the detection of specific pathogens.
Current ISO directives have been published for the analysis of:
Staphylococcus aureusE. coliEnterobacteriaListeria monocytogenesMicroorganismsBacillus cereusYeasts and mouldsClostridium perfingensSalmonella spp.
The graphical protocol description will help the microbiologist to apply the official ISO methods without having to read a lot of text.
Since new directives are added every year and existing methods changed, please check our website (www.scharlau.com) for possible updates on this brochure.
Apart from the media mentioned in this leaflet, Scharlau offers a full range of standard and chromogenic media. Both in dehydrated and pre-pared form.
Please check our website for the latest editions.
CONSIDERATIONS:TO chOOSE ThE dIluTION fluId, SEE ISO 6887 (PrEParaTION Of
TESTS SamPlES INITIal SuSPENSION aNd dEcImal dIluTIONS fOr
mIcrObIOlOgIcal ExamINaTION) Or 8261 aNd ThE SPEcIfIc
INTErNaTIONal STaNdard dEalINg wITh ThE PrOducT uNdEr
ExamINaTION.
gENEral dIluENTS arE SalINE PEPTONE waTEr/maxImum rEcOvEry
dIluENT (rEf. 2-510), buffErEd PEPTONE waTEr (rEf. 2-277) aNd OThErS
lIkE PEPTONE waTEr (rEf. 3-156).
www.scharlab.com [email protected]
Baird Parker Agar Base 01-030-500 01-030-100 064-BA1033 Egg’s Yolk Telurite Sterile Emulsion 06-026-100 Baird Parker Medium 064-PA0008 064-PR0008Lyophilized Rabbit Plasma (Coagulase Test) 064-PLA-CO Brain Heart Infusion Broth (Coagulase Test) 02-599-500 02-599-100 064-TA0143
Staphylococcus aureus and other species EN-ISO 6888-1:2000Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species). Part 1: Technique using Baird-Parker Agar Medium. Isolation medium ConfirmationSpread inoculum over surface of Baird Parker Agar CoagulaseIncubation: 37 ±1°C for 24-48 h
S. aureus Discard
isolation
–+
EN-ISO 6888-2:2000 Enumeration of coagulase-positive staphylococci. (Staphylococcus aureus and other species).Part 2: Technique that use Rabbit Plasma Fibrinogen (RPF) Agar Medium. Isolation medium ConfirmationMass inoculation technique of Rabbit Plasma Fibrinogen Agar Is not necessaryIncubation: 35-37 ±1°C for 18-24 h Incubate for a further 24 hours if necessary
Baird Parker Agar Base 01-030-500 01-030-100 064-BA1033 Egg’s Yolk Telurite Sterile Emulsion 06-026-100RPF Supplement Lyophilized 064-TA-0155Baird Parker RPF Base 064-BA1028 RPF Supplement Lyophilized 064-TA-0155 Baird Parker RPF Agar 064-BA0RPF(1) 064-PA0145
isolation Confirmation
+
–
+–S. aureus
OthersDiscard
Coagulase37 ±1°C up to 24 h
0,1 ml
BHI37 ±1°C - 24 ± 2 h
0,1 ml
samplE dilution
0,1 ml
samplE dilution
1 ml
1 ml
(1)Set: 4 bottles of Baird Parker RPF base + 4 vials of RPF Suppplement
Tel. +34 93 745 64 00 Fax +34 93 715 27 65
isolation
TBX
–
+
Blue: E. coli
Other color: Discard
TBX
Escherichia coli EN-ISO 16649-2:2001 Horizontal method for the enumeration of ß-glucuronidase-positive Escherichia coli. Part 2: Colony-count technique at 44°C using 5-bromo-4-chloro-3-indolyl ß-D-glucuronide.
Selective isolation mediumTryptone Bile X-Glucuronide Agar (TBX) Mass inoculation Incubation: 44°C for 18-24 hIn case of suspected damaged cells, incubate in advanced at 37°C for 4 h.
TBX Agar 01-619-500 01-619-100 064-BA0945 064-PA0945
samplE dilution
TBX15 ml
1 ml
1 ml
www.scharlab.com [email protected]
Buffered Peptone Water 02-277-500 02-277-100 064-BA0486 064-TA0100 EE Broth 02-064-500 02-264-100 064-TA0189 Violet Red Bile Dextrose Agar (VRBD) 01-295-500 01-295-100 064-BA1011 064-TA0147 064-PA0046 064-PR0006Oxidase Test Sterile Swabs 06-119-025Oxidase Reagent in powder 06-057-005 Glucose Bromocresol Purple Agar 01-502-500 01-502-100 064-TA0129*Nutrient Agar 01-144-500 01-144-100 064-PA0059
Enterobacteriaceae ISO 8523:1991General guidance for the detection of Enterobacteriaceae with pre-enrichment. Pre-enrichment medium Enrichment medium Isolation medium ConfirmationBuffered Peptone Water Buffered Glucose-Brilliant Violet Red Bile Oxidase Green Bile Broth (EE Broth) Dextrose Agar (VRBD)Incubation: 35-37°C for 16-20 h Incubation: 35-37°C for 18-24 h Incubation: 35-37°C for 24 ± 2 h Glucose fermentation (Glucose Bromocresol Purple) Incubation: 37°C for 24 ± 2 h
Buffered Peptone Water
10 ml
+
– Discard
Oxidase
Other color > Discard
+
+ Discard
– Enterobacteriaceae
EnriChmEnt isolation ConfirmationprE-EnriChmEnt
1 ml
Nutrient Agar
Glucose fermentation
+ Enterobacteriaceae
* Please check availability
Tel. +34 93 745 64 00 Fax +34 93 715 27 65
VRBL
+ Red 0,5mm ø Count
– Discard
15 ml
isolation Confirmation
Coliforms EN-ISO 4832:1991General guidance for the enumeration of coliforms - Colony-count technique.
Isolation medium Characteristic coloniesMass inoculation with Violet Red Bile Lactose Agar (VRBL) Violet red with ø ≥ 0,5 mmIncubation: 30°C, 35°C or 37°C for 24 ± 2 h
Violet Red Bile Lactose Agar (VRBL) 01-164-500 01-164-100 064-BA0485 064-TA0485 064-PA0049 064-PR0018
samplE dilution
1 ml
1 ml
www.scharlab.com [email protected]
+ (See below)
– Discard
Fraserhalf-
concentrated
225 ml Fraser
Oxford
Palcam
CatalaseAdd 1 drop of hydrogen
peroxid
Gram Stain (Gram +)
Motility Broth(25°C for 48 h until 5 days
if it is necessary)
Haemolysis in Blood Agar n. 2(37°C - 24 ± 2 h)
Ramnose(35°C or 37°Cuntil 5 days)
Xylose(35°C or 37°Cuntil 5 days)
CAMP testin Blood Agar n. 2
isolation
Confirmation
EnriChmEnt
Listeria EN-ISO 11290-1:1997Horizontal method for the detection and enumeration of Listeria monocytogenes. Part 1: Detection method. Enrichment media Isolation media ConfirmationPrimary enrichment Spread inoculum over surface of PALCAM Agar and OXFORD Agar Genus confirmation:in Half-concentrated Fraser Broth Incubation: Aerobic or microaerobic 30°C, 35°C or 37°C for 24 ± 2 h CatalaseIncubation: 30°C for 24 ± 2 h and continue for another 24 ± 2 h if there are no colonies or they are small Gram MotilitySecondary enrichment: Specie confirmation:0,1 ml of primary culture Haemolysisin 10 ml of Fraser Broth Fermentation of:Incubation: 35 or 37°C for 48 ± 2 h • Ramnose • Xylose CAMP testNote: This standard has been revised. The new version include as an isolation method a chromogenic medium, like ALOA Agar, or other lecithin based ones, and also another medium of the laboratories choice.
– Discard
+ (See below)
TSYEA(35°C or 37°C for 18 or 24 h)0,1 ml
Fraserhalf-
concentrated
225 ml
TSYEB
Tel. +34 93 745 64 00 Fax +34 93 715 27 65
Palcam– Discard
isolation
Listeria EN-ISO 11290-2:2000Horizontal method for the detection and enumeration of Listeria monocytogenes. Part 2: Enumeration method. Dilution and revivification Isolation medium ConfirmationBuffered Peptone Water or Fraser Broth Base Spread inoculum over surface of PALCAM Agar See EN-ISO 11290-1:1997obtain 1/10 dilution Incubation: Aerobic or microaerobic 35°C or 37°C for 24 ± 2 h Revivification: 20°C for 1 h and continue for another 24 ± 2 h if there are no colonies or they are small Note: This standard is being revised. The new version it is envisaged, will include as an isolation method a chromogenic medium, like ALOA Agar, or other lecithin based ones, and also another medium of the laboratories choice. Warning!: With the object of safeguarding the health of the laboratory staff, it is recommended that care is taken and the tests for Listeria are performed in properly equipped labs under the supervision of qualified microbiologists and that all contaminated materials are discarded safely. In particular it is recommended that female personnel are advised of the risk to the developing foetus if the mother is infected after exposure to Listeria monocytogenes. National legislation may establish the exact requirements.
+ (See below)
Half-concentrated Fraser Broth (1) 064-BA5002 Fraser Base Broth (1) 02-496-500 02-496-100 Listeria Selective Suppl. for Fraser 06-111CASE Ferric Ammonium Citrate 06-112CASE Fraser Broth 064-TA1125 Oxford Agar 064-PA0071Oxford Base Agar 01-471-500 01-471-100Oxford Selective Supplement 06-109CASE Palcam Agar 064-PA0070Palcam Base Agar 01-470-500 01-470-100Palcam Selective Supplement 06-110CASE Listeria Chromogenic Medium acc. ISO 11290 064-PA0LCMCatalase AC07461000 064-TA9999* Crystal Violet, solution according Gram 06-029-100 06-0291000 Lugol’s solution 06-030-100 06-0301000 Bleaching agent, solution according Gram 06-031-100 06-0311000 Safranine, solution according Gram 06-032-100 06-0321000 Motility Broth 064-TA9998*Blood Base Agar n. 2 01-505-500 01-505-100Defibrinated Sheep Blood (2) 064-SS0003 Blood Agar n. 2 064-PA9997Ramnose Test 064-TA9996* Xylose Test 064-TA9995*TSYEB 02-498-500 02-498-100 064-TA9993*TSYEA 064-PA9994*TSYEB (3) 02-498-500 02-498-100 064-TA9993*Agar- Agar 07-490-500 07-490-100
Buffered Peptone Wateror Fraser Broth Base
0,1 ml
dilution and rEvivifiCation
Buffered Peptone Water 02-277-500 02-277-100 064-BA6012 064-BA0486 064-TA0100 Fraser Base Broth (1) 02-496-500 02-496-100 Listeria Selective Suppl. for Fraser 06-111CASE Ferric Ammonium Citrate 06-112CASE Fraser Broth 064-BA5001 064-TA1125 Palcam Agar 064-PA0070Palcam Base Agar 01-470-500 01-470-100Palcam Selective Supplement 06-110CASE
TSYEA(35°C or 37°C
for 18 h or 24 h)
(1) Add 1 vial of 06-111CASE and 2 vials of 06-112CASE to 02-496, to obtain 1 l of half-concentrated Fraser Broth; (2) Only available in spanish market;(3) Add to TSYEB 9-18g/l of Agar-Agar to obtain TSYEA formulation * Please check availability
See on page before, the confirmation references of EN-ISO 11290-1:1997
www.scharlab.com [email protected]
Enumeration
isolation
Microorganisms EN-ISO 4833:2003 Horizontal method for the enumeration of microorganisms. Colony-count technique at 30°C.
Isolation mediumPlate Count Agar (PCA) Mass inoculation Incubation: 30°C for 72 ± 3 h
15x15
Plate Count Agar 01-161-500 01-161-100 064-BA1005 064-TA0325 064-PA0024 064-PR0001Plate Count Skim milk Agar (1) 01-412-500 01-412-100
15 ml1 ml
1 ml
samplE dilution
MYP
isolation Confirmation
Bacillus cereus En-ISO 7932:1998Horizontal method for the enumeration of presumtive Bacillus cereus. Colony-count technique at 30°C. Isolation medium ConfirmationSpread inoculum over the surface of Mannitol-Egg Yolk-Polymyxin Agar (MYPA) ß-haemolysis test on Sheep Blood Agar Incubation: 30°C for 18-24 h (Blood Agar n. 2)Incubate for further 24 hours if colonies are not easily visible Incubation: 30°C for 24 ± 2 h
MYP Base Agar (Bacillus Cereus Agar) 01-262-500 01-262-100 064-BA2129 Egg’s Yolk Sterile Emulsion 06-016-100 Polymixin B Sulfate Selective Supplement 06-021CASEMYP Agar (Bacillus Cereus Agar) 064-PA0032Blood Base Agar n. 2 01-505-500 01-505-100Defibrinated Sheep Blood (1) 064-SS0003 Blood Agar n. 2 064-PA9997
samplE dilution
Sheep Blood Agar
0,1 ml
0,1 ml
+ ß-haemolysis
(1) Only available in spanish market
(1) When milk and dairy products are examinated
Tel. +34 93 745 64 00 Fax +34 93 715 27 65
Penicilium or othersCGA
Yeasts
isolation Confirmation
Yeasts and moulds ISO 7954:1987General guidance for enumeration of yeasts and moulds. Colony-count technique at 25°C.
Isolation medium ConfirmationChloramphenicol Glucose Agar, CGA (Yeast Extract Glucose Chloramphenicol Agar, YGC) Microscopical observation Mass inoculation technique if necessarylncubation: 25°C with readings on day 3, 4 and 5
CGA Agar (YGC) 01-366-500 01-366-100 064-BA9992 064-PA9992
samplE dilution
15 ml1 ml
1 ml
www.scharlab.com [email protected]
isolation Confirmation
TSC
Clostridium perfringens EN-ISO 7937:2005Horizontal method for the enumeration of Clostridium perfringens. Colonies enumeration technique.
Isolation medium Confirmation: Two optionsMass inoculation (poured-plate technique) Option 1:in Sulfite-Cycloserine without Egg Yolk (SC) • Lactose Sulfite Broth (LS) (This was originally designated “Egg-Yolk-free Tryptose-Sulfite-Cycloserine without Egg Yolk”, TSC) Incubation: Incubate anaerobically 46°C for 18-24 h in bathIncubation: Under anaerobic conditions 37°C for 20 ± 2 h With prior inoculation in Thioglycollate Broth Incubation: Incubate anaerobically 37°C for 18-24 h Option 2: • Motility-Nitrate Medium Stab-inoculate colonies into freshly deaerated medium 37°C for 24 h Incubation: Incubate anaerobically 37°C for 24 h • Lactose-Gelatine Medium Incubation: Incubate anaerobically 35°C or 37°C for 24 h plus another 24 h to check Gelatin test, previous cooling 5°C for 1 h
TSC Agar Base 01-278-500 01-278-100 064-BA6016 Cycloserine Selective Supplement 06-116CASETSC Agar 064-BA6020 Thioglicollate 03-187-500 03-187-100 064-TA0134Lactose Sulfite Base Broth 02-519-500 02-519-100 Ammonium Ferric Citrate 06-114CASESodium Metabisulfite solution 06-113CASELactose Sulfite Broth 064-TA0846Motility-Nitrate Medium 03-612-500 03-612-100 064-TA9991* Lactose-Gelatin Medium 03-632-500 03-632-100 064-TA9990* A Reagent for Nitrates Reduction test 06-003-100B Reagent for Nitrates Reduction test 06-004-100
samplE dilution
15 ml1 ml
1 ml
Option 1:
Option 2:
Thioglycollate Broth
+Lactose Sulfite Broth (LS)
– Lactose Sulfite Broth
5 drops
+Motility-Nitrate Medium
+Lactose Gelatin Medium
TSC
* Please check availability
Tel. +34 93 745 64 00 Fax +34 93 715 27 65
Confirmation
+TSI Agar
Salmonella spp. EN-ISO 6579:2003Horizontal method for the detection of Salmonella spp.
Pre-enrichment medium Enrichment media Isolation media ConfirmationBuffered Peptone Water Rappaport-Vassiliadis Soya Broth (RVS) Isolation in Xylose Lysine Biochemical Confirmation Incubation: 37°C for 18 ± 2 h Incubation: 41,5°C for 24 ± 3 h Deoxycholate Agar (XLD) Triple Sugar iron Agar ( TSI) and a second agar Urea Agar choosed by the laboratory Lysine Descarboxilation Incubation: 37°C for 24 ± 3 h ß-galactosidase Voges-Proskauer Tetrathionate Muller-Kauffmann Broth Isolation in Xylose Lysine Indole with Novobiocine (MKTTn) Deoxycholate Agar (XLD) Incubation: 37°C for 24 ± 3 h Incubation: 37°C for 24 ± 3 h and a second agar Serological Confirmation choosed by the laboratory Antigens Confirmation O, Incubation: 37°C for 24 ± 3 h H and Vi
0,1 mlRappaport-VassiliadisSoya Broth
1 mlMKTTn
Brilliant Green Agar (BGA)Hektoen AgarSalmonella Shigella Agar (SS)Chromogenic
XLDand other second agarchosen by the laboratory
isolation
–Urea Agar
+Lysine
descarboxilation
–ß-galactosidase
+Voges-Proskauer
–Indole
EnriChmEnt
25 g or 25 ml
0,1 ml
prE-EnriChmEnt
Brilliant Green Agar (BGA)Hektoen AgarSalmonella Shigella Agar (SS)Chromogenic
XLDand other second agarchosen by the laboratoryBuffered
Peptone Water225 ml
See the ordering information, on the next page.
www.scharlab.com [email protected]
Microorganisms Enterobacteriaceae E. coli S. aureus Salmonella Yeasts and moulds Other parameters/ /Coliforms L. monocytogenes Commentsbottled drinking water (microbiological rules at the bottle) • Coliforms • Enterococci, P. aeruginosa, clostridiaBottleddrinkingwater(waterrulesfromwatersource) • Coliforms • fecal enterococci, P. aeruginosa, clostridiaDrinkingwaterforpublicconsumption • Coliforms • Enterococci,C.perfringensBroth,consommé,soupsandcreams • • C.perfringens,B.cereusSweetsandchewinggums • Enterobacteriaceae YeastsandmouldsRefrigeratedandfrozenmeat • Enterobacteriaceae • • • C.perfringens,ShigellaFreshpoultryandrabbitmeat • Enterobacteriaceae • • C.perfringens,ShigellaMincedmeatfromthebutcher’s • • C.perfringens,ShigellaMincedmeatfromthemeatindustry • • • • Meatderivatives(ham,coldmeat,sausages) • • •Butcher’sproducts(cookedandpasteurized) • Enterobacteriaceae • • • Shigella, anaerobicButcher’sproducts(incans) • Enterobacteriaceae • • • Shigella, anaerobicHamandcoldham Enterobacteriaceae • • Shigella,Cl.sulfitereductorsRoastedjoinandcoldbacon Enterobacteriaceae • • Shigella,Cl.sulfitereductorsCookedleanmeatandandcoldleanmeat Enterobacteriaceae • • Shigella,Cl.sulfitereductorsCereals • Coliforms • • YeastsandmouldsBreakfastcereals • • • Yeastsandmoulds B.cereusBeer Enterobacteriaceae • MouldsGroupA:Non-heat-treatedprocessedfoodandheat-treated • Coliforms • • • l. monocytogenes 1processedfoodwithnon-heat-treatedingredientsGroupB:Heat-treatedprocessedfood • Coliforms • • • l. monocytogenes 1GroupC:Sterilizedprocessedfood 1GroupD:Packedfoodwithrawvegetables • • • l. monocytogenes 1Herbsandspices • • SulfitereductorsCannedfood • Enterobacteriaceae • • • Shigella,Cl.sulfitereductorsCurdmilkandcoagulantenzymes • Enterobacteriaceae • • • Yeastsandmoulds Cl.sulfitereductorsSpecialfoodsupplementsforbreastfeeding • Coliforms • • • YeastsandmouldsFruitsandvegetables • Coliforms • • YeastsandmouldsFrozenfruitsandvegetables • Coliforms • • • Yeastsandmoulds Shigella,Sulfitereductors,aerobicpsicrofilic
Plainbiscuits • Enterobacteriaceae • • • • B.cereusNon-plainbiscuits • Enterobacteriaceae • • • • B.cereusEdiblegelatins • • • • C.perfringensEdiblefats • Yeastsandmoulds ArizonaSemolinaandflour • • • MouldsIce-creamsandfrozenproducts • Coliforms • • l. monocytogenesSyrups • Enterobacteriaceae • •
Buffered Peptone Water 02-277-500 02-277-100 064-BA6012 Rappaport Vassiliadis Caldo 02-379-500 02-379-100 064-TA0198 Tetrathionate Muller-Kauffmann Base Broth 02-335-500 02-335-100 Brilliant Green-Novobiocin Supplement 06-017CASEIodine-iodide solution for Muller Kauffman 064-V11108 Tetrathionate Muller-Kauffmann Broth 064-TA1024 Iodine-iodide solution for Muller Kauffman 064-V11108 Xylose Lysine Deoxycholate Agar (XLD) 01-552-500 01-552-100 064-PA0034Brilliant Green Agar (BGA) 01-309-500 01-309-100 064-PA0045Hektoen Agar 01-216-500 01-216-100 064-PA0014Salmonella Shigella Agar (SS) 01-171-500 01-171-100 064-PA0028Salmonella Plus Chromogenic Agar (Colorex®) 064-PA0083TSI Agar 01-192-500 01-192-100 064-TA0177 Urea Base Agar 01-261-500 01-261-100 Urea Sterile Solution 40% 06-083-100 Urea Agar 064-TA0148 Lysine Descarboxilation 064-TA0125* ß-galactosidase (ONPG) 064-TA0203 Methyl Red Voges-Proskauer Broth (MRVP Broth) 02-207-500 02-207-100 064-TA0261Barrit’s Reagent for Voges-Proskauer Test 06-027-100 O’Meara’s Reagent for Voges-Proskauer Test 06-006-100 Indole Test 064-TA0132 Kovac’s reagent for Indole Test 06-018-100
Colorex® is the trademark for CHROMagar™ prepared media.* Please check availability
Food products determinations
1 Without pathogenic agents
Tel. +34 93 745 64 00 Fax +34 93 715 27 65
Microorganisms Enterobacteriaceae E. coli S. aureus Salmonella Yeasts and moulds Other parameters/ /Coliforms L. monocytogenes CommentsRawcow’smilkheat-treateddairyindustry • Rawcow’smilkfornon-heat-treateddairyproducts • • Rawcow’smilk,non-heat-treatedbetween36hours • Rawbuffalo’smilkforheat-treateddairyproducts • Rawbuffalo’smilkfornon-heat-treateddairyproducts • • Rawgoatandsheep’smilkforheat-treateddairyproducts • Rawgoatandsheep’smilkfornon-heat-treateddairyproducts • • Rawcow’smilkfordirectconsumption • • •Pasteurizedorheat-treatedcow’smilk • Pasteurizedmilk • Coliforms • l. monocytogenesSterilizedandUHTmilk • Coliforms Powderedmilk Coliforms • • l. monocytogenesCheesefromraworheat-treatedmilk • • • l. monocytogenesCreamcheese(fromheat-treatedmilk) Coliforms • • • l. monocytogenesCottagecheese • • l. monocytogenesPasteurizedcheese Enterobacteriaceae • • • l. monocytogenes ShigellaCreamcheese Enterobacteriaceae • • • l. monocytogenes Shigellacream, grated or powdered cheese or with vegetable ingredients Enterobacteriaceae • • • l. monocytogenes ShigellaFrozendairyproducts • Coliforms • • l. monocytogenesLiquiddairyproducts Coliforms • l. monocytogenesSterilizeddairyproducts,UHTliquidsorjellies. • Liquiddairyproducts,heat-treated,non-sterilized • Coliforms • l. monocytogenesandnon-fermented(cream,condensedmilk…)Butterfrommilkorpasteurizedcream Coliforms • l. monocytogenes ShigellaCurdcheese • Enterobacteriaceae • • • l. monocytogenes ShigellaYogurt Coliforms • • l. bulgaricus, S. thermophilusHoney • Enterobacteriaceae • • Moulds ShigellaEggproducts • Enterobacteriaceae • •Cakesandpastries • • Yeastsandmoulds Shigella,Cl.sulfitereductorsCrispsandsnackfood Enterobacteriaceae • • Streptococcusfaecalis,ShigellaFresh,salted,refrigeratedandfrozenfishproducts • Enterobacteriaceae • ShigellaCookedfishproducts • Enterobacteriaceae • • ShigellaCannedseafoodwithvinegar • Enterobacteriaceae • • ShigellaAnchoviesinoil • Enterobacteriaceae • AnaerobesSeafood Coliforms • •Dried,saltedfishproducts • Enterobacteriaceae • ShigellaSmokedfish • Enterobacteriaceae • • ShigellaSmokedsalmon,codandothers • Coliforms • • l. monocytogenesSmokedherringandanchoviesinbrine • Coliforms • l. monocytogenesSmokedsalmon,vacuum-packed • Coliforms • • l. monocytogenesCookedseafood Coliforms Seafoodwithshell • •Seafoodwithoutshell • Coliforms • • •Crabmeat • •Packedsalt • 1Tablesauces: Coliforms Friedtomato • Shigella,B.cereusKetchup • Enterobacteriaceae • ShigellaMayonnaise • Enterobacteriaceae • ShigellaMustard • Enterobacteriaceae • ShigellaOtherssauces Enterobacteriaceae • ShigellaTeaandderivatives • • • • Shigella,B.cereusNougatandmarzipan Enterobacteriaceae • • • Yeastsandmoulds Shigella1 Without pathogenic agents
Food products determinations
www.scharlab.com [email protected]
CA
T-FO
OD
07 (7
-200
7)
Culture Media Handbook Edition no. 9
The latest edition no. 9 is ready. Over 276 pages with helpful information and photos of our media. Spiral bound for easy and space-saving manipulation on the work bench. International edition written in English language. Cross reference charts available separately in Excel format. New MSDS can be downloaded from our website: www.scharlau.com. Contains over 350 different products.
Microbiology catalogues
1: Dehydrated culture media handbook 2: NEW Prepared culture media for microbiology leaflet 3: NEW Microbiological water testing leaflet 4: Clinical microbiology leaflet 5: NEW Food analysis leaflet 6: RPF leaflet 7: NEW CHROMagar™ leaflet 8: Colinstant leaflet 9: Supplements leaflet10: Dehydrated/prepared culture media and culture media-supplements poster
1 2 NEW 3 NEW
8 9 10
4 5 NEW 6 7 NEW
Technical documentation
Scharlab [email protected]. +34 93 745 64 00Fax +34 93 715 27 65
