JOURNAL OF CLINICAL MICROBIOLOGYVOLUME 3 NUMBER 6 JUNE 1976

EDITORIAL BOARDALBERT BALOWS, Editor-in-Chief (1979)

Center for Disease Control,Atlanta, Ga.

LORRAINE FRIEDMAN, Editor (1979)Tulane University Medical School,

New Orleans, La.

HENRY D. ISENBERG, Editor (1979)Long Island Jewish-Hillside Medical Center,

New Hyde Park, N. Y.NATHALIE J. SCHMIDT, Editor (1980)

California Department of Health,Berkeley, Calif

Daniel Amsterdam (1977)Libero Ajello (1976)William B. Cherry (1978)Walter R. Dowdle (1976)V. R. Dowell, Jr. (1977)Richard W. Emmons (1977)John C. Feeley (1977)James D. Fenters (1976)Sydney M. Finegold (1976)Maxwell Finland (1977)Herman Friedman (1978)Thomas L. Gavan (1977)Morris A. Gordon (1978)Dieter Groschel (1977)Leonor D. Haley (1977)James L. Hardy (1978)Melvin T. Hatch (1978)Harry R. Hill (1977)

Lillian V. Holdeman (1976)Milton Huppert (1978)S. S. Kalter (1978)Leo Kaufman (1977)George E. Kenny (1976)George P. Kubica (1976)Howard W. Larsh (1977)James D. MacLowry (1977)Harold Markowitz (1977)William J. Martin (1977)John M. Matsen (1977)Joseph L. Melnick (1976)Willum I. Metzger (1977)Maurice A. Mufson (1977)Barbara G. Painter (1977)Demosthenes Pappagianis (1977)Robert H. Purcell (1977)William E. Rawls (1977)

Glenn D. Roberts (1977)Jon E. Rosenblatt (1977)Margarita Silva-Hutner (1977)James W. Smith (1977)Louis DS. Smith (1978)Peter B. Smith (1976)Alex C. Sonnenwirth (1977)Welton I. Taylor (1977)John H. Thompson, Jr. (1977)Richard C. Tilton (1978)Marvin Turck (1977)Alexander von Graevenitz (1977)Kenneth W. Walls (1977)Lewis W. Wannamaker (1978)Robert E. Weaver (1978)Irene Weitzman (1978)Robert P. Williams (1978)Marion E. Wilson (1977)

Robert A. Day, Managing EditorGisella Poliock, Director of Editorial Services

Leslie Frymire, Production Editor1913 I St., N. W., Washington, D.C. 20006

EX OFF]Helen R. Whiteley, President (1975-1976)

J. Mehsen Joseph, Secretary

The Journal of Clinical Microbiology, a publication of theAmerican Society for Microbiology, 1913 I St., N. W., Wash-ington, D.C. 20006, is devoted to the dissemination of newknowledge concerning the applied microbiological aspects ofhuman and animal infections and infestations, particularlyregarding their etiologic agents, diagnosis, and epidemiology.Papers dealing with antibiotics and antimicrobial agents andchemotherapy, with fundamental aspects of infection andimmunity, and with food or dairy microbiology fall within thescope of other ASM publications. The Journal is publishedmonthly, and the twelve numbers are divided into two volumesper year. The nonmember subscription price is $50 per year.The member subscription price is $13 per year. Single copiesare $5. Correspondence relating to subscription, reprints,

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Author IndexBlattner, W. A., 593Blazevic, Donna J., 556Brashear, Mary D., 640Brock, Susan, 582

Chamberland, Mary, 582Clark, W. A., 632Clinard, Elva H., 604

Dart, R. K., 635

Evans, Charles A., 576

Fedorko, J., 599Fossieck, B. E., Jr., 599Fraumeni, J. F., Jr., 593

Gabridge, Michael G., 560Giard, D. J., 593Gunnell, M., 593

Hall, M. Marsha, 643Hebert, G. A., 609

Hines, Paula, 640

Ilstrup, Duane M., 643Ishizaki, Hiroshi, 545Iwatsu, Tokio, 545

Kalmakoff, J., 637Kaplan, N. M., 593Kariya, Hideo, 545

Lambe, Dwight W., Jr., 586Lubiniecki, A. S., 593Lunceford, Carl D., 613Lund, Maryls E., 556Lyons, Robert W., 582

Mason, T., 593Moroz, David A., 586Myers, Donald M., 548

Nakamura, Yoichi, 545

Otto, L. A., 566

Parker, R. H., 599Parkinson, A. J., 637Pickett, M. J., 566Prince, Alfred M., 626

Rubin, Sally J., 582

Saunders, George C., 604Shepard, Maurice C., 613Smith, Rodney F., 640Stevens, Robert J., 576Stretton, R. J., 635

Triantafellu, N., 593Tzianabos, T., 609

Vnek, John, 626

Washington, J. A., II, 643Wheat, Robert, 545White, L. A., 609Williams, D. N., 556Wong, James, 640

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Friday and Saturday, October 15 and 16, 1976Sheraton-Park Hotel, Washington, D.C.

This Conference will focus attention on newer developments in laboratory immu-nology which are directly relevant to clinical patient care as well as to a wide varietyof biomedical disciplines, including antimicrobial immunity, autoimmunity, trans-plantation immunity, and cancer immunology.

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AUTHOR INDEX

VOLUME 3

Achanzar, David, 206Aikawa, T., 206Akiya, F. I., 21Alexander, David N., 42Alexander, Jane J., 381Allen, Alfred M., 465Alley, Cynthia C., 180Alon, Shlomit, 227Altmann, G., 390Amdur, B., 474Anderson, Richard A., 382

Bae, H. C., 211Bagley, L. R., 513Bahnemann, Hans G., 209Baker, C. N., 425Barden, Gertrude, 246Barile, Michael F., 110Barnard, Tom, 382Baron, Ellen S., 330Baron, Samuel, 223Bartenstein, Lynne, 8Bartlett, John G., 133Bartlett, Raymond C., 327Ben-Moshe, Hana, 227Bernstein, R. S., 474Bessudo, David M., 486Bettini, T. Manlio, 203Beutner, Karl R., 469Blakemore, William S., 264Blattner, W. A., 593Blazevic, Donna J., 313, 556Blue, J. L., 149Bodey, Gerald P., 14Boncyk, L. H., 463Bradbury, Janet M., 449Brady, Margaret I., 524Brashear, Mary D., 640Brasier, Francoise, 309Brock, Susan, 582Brooks, John B., 34, 180, 239Brosi, B. J. M., 541Brown, J., 149Bruun, Johan N., 264Buckingham, Judy M., 443Burgdorfer, W., 51Burson, G. Gene, 251, 258

Cahan, D., 390Cannon, Hubert, 465Cannonico, P. G., 513Carmody, Patricia, 469Carter, G. R., 344Casper, E. A., 51Chalgren, Sarah L., 359Chamberland, Mary, 582Chambers, R. W., 364Chester, Brent, 119

Chow, Anthony W., 128Chu, Fred C., 402Clark, W. A., 632Clinard, Elva H., 604Cole, Francis E., Jr., 460Cole, John R., Jr., 537Coleman, R. Marie, 281Corbett, Dorothy S., 306Coriell, Lewis L., 264Couch, Robert B., 309Cox, James H., 96Cummins, A. C., 5Cunningham, Patrick J., 128

D'Arcangelis, Dora C., 338Dajani, Adnan S., 318Dart, R. K., 635David, Hugo L., 414Davis, R. B., 149Deibel, Rudolf, 397DeWitt, Wallis E., 486DeYoung, Donald R., 47Diaz, Ramon, 203DiSalvo, Arthur F., 306Dizon, Delia, 211Dobos, P., 373Dorn, Gordon L., 251, 258Dowdle, Walter R., 233Dowell, V. R., Jr., 291Downie, Jean C., 233Drake, C. R., Jr., 359Duncan, W. Christopher, 14

Edberg, Stephen C., 49Eddy, Gerald A., 460Ederer, Grace M., 42Edwards, Earl A., 339Edwards, L. R., 501Elin, R. J., 21Evans, Charles A., 576Evans, G. L., 496

Facklam, R. R., 501Falco, Eleanora B., 453Farmer, J. J., III, 206, 350Fedorko, J., 599Feeley, John C., 486Finkelstein, Richard A., 382Flower, Margaret, 246Fossieck, B. E., Jr., 599Fraumeni, J. F., Jr., 593Freeburg, Pat W., 443Fuchs, Peter C., 75Furminger, I. G. S., 524

Gaafar, Hassan A., 438Gabridge, Michael G., 560Galphin, Judith C., 233

Gangarosa, Eugene J., 143Gelbart, Sheldon M., 62Gerichter, Ch. B., 390Gerna, G., 364Gershman, M., 214Giard, D. J., 593Gilman, Robert H., 456Gorbach, Sherwood L., 133Gordon, L. P., 102Goss, W. A., 406Grabowski, Marion W., 110Graves, S. R., 72Gray, Samuel B., Jr., 1Grove, S. B., 474Green, James H., 1Greenberg, J., 324Greenberg, Stephen B., 309Greenfield, Brian, 327Greenlee, Herbert B., 62Grillner, Lena, 86Gross, Peter A., 246Gunnell, M., 593Guze, Lucien B., 128

Hall, M. Marsha, 212, 643Harrell, W. Knox, 1Hawkins, Jean E., 453Haynes, John R., 251, 258Hebert, G. A., 609Hennessy, Joan N., 385Hentges, David J., 218Hickman, F. W., 350Higbee, James W., 137Hilderbrand, Richard L., 339Hines, Paula, 640Hinton, Norman A., 385Ho, Monto, 272Hochstein, H. D., 21Holdeman, Lillian V., 432Holder, Ian A., 175Hollick, Gary E., 302Hollis, D. G., 425Hornick, Richard B., 456Huppert, M., 186

Iannelli, Domenico, 203Iida, H., 206Ilstrup, Duane M., 212, 643Irwin, Gilbert R., 465Ishizaki, Hiroshi, 545Ito, H6mu, 91Iwatsu, Tokio, 545

Jacobs, Nicholas J., 287Jenkin, H. M., 72Jerris, Robert C., 506Jimenez-Ulate, F., 359Johns, Ronald W., 137

ii AUTHOR INDEX

Johnson, Dwight R., 533Johnson, Eugene A., 105Johnson, James E., 157, 334Johnson, Joyce B., 402Johnson, J. L., 359Jones, Dan, 309Jones, James R., 382Jones, Ronald N., 75Jones, W. D., Jr., 324Jones, Wallis L., 480Jordan, F. T. W., 449

Kalmakoff, J., 637Kalter, S. S., 463Kaplan, M. M., 593Kaplan, William, 272Kariya, Hideo, 545Karlson, Alfred G., 47Katz, David, 227Kaufman, Leo, 191Kaul, Bansi L., 469Kaye, Donald, 67Keen, Barbara M., 318Kellogg, Douglas S., Jr., 34Kennett, Margery L., 528Kenyon, R. H., 513Klein, George C., 458Knight, Ralph A., 67Knight, S. T., 72Knight, Vernon, 309

Lambe, D. W., Jr., 281, 506, 586Larghi, 0. P., 26Larsson, Lennart, 81Laslie, W. Wayne, 281Laver, W. Graeme, 233Lewis, F. A., 528Lewis, Jay F., 381Littlejohn, Dewitt C., 402Lombard, G. L., 291Lubiniecki, A. S., 593Lunceford, Carl D., 613Louie, Thomas J., 133Lukert, P. D., 149Lund, Maryls E., 556Luoma, M. K., 21Lutticken, Doris, 533Lutticken, Rudolf, 533Lyons, Robert W., 582

McBride, Charles M., 14McGrath, M. B., 406McBride, Mollie E., 14McCloud, C. J., 364McGarrity, Gerard J., 264Macks, Gerald C., 421MacLowry, James D., 421Mardh, Per-Anders, 81Marks-Hellman, Sherry, 272Martin, William T., 486Marucha, P. T., 474Mason, T., 593Matsen, John M., 42, 105Mazens, Mary, 327Merritt, Katharine, 287

Merson, Michael H., 143, 486Merz, W. G., 496Michaud, R. N., 406Mihailescu, Cornelia, 211Millstein, C. H., 463Milner, E. C. B., 21Morris, George K., 143, 486Morse, C. Dwayne, 34Morse, Stephen A., 8Moroz, David A., 586Murphy, Brian R., 223Myers, Donald M., 548

Nakamura, Yoichi, 545Nebel, A. E., 26

Oberhofer, Thomas R., 137Oblack, Donna, 175Ogra, Pearay L., 469Ormsbee, R. A., 51Oriel, Christine A., 449Orr, Henry C., 402Otto, L. A., 566Owens, Dwight R., 218

Palutke, Waldemar A., 77Parisi, Joseph T., 519Parker, R. H., 599Parkinson, A. J., 637Patton, Charlotte M., 143Peacock, M. G., 51Pedersen, Carl E., Jr., 113, 460,

513Penner, John L., 385Peterson, Martin W., 376Petricciani, John C., 402Philip, R. N., 51Phillips, Betty J., 272Phillips, S. E., 291Pickett, M. J., 566Porschen, Richard K., 161Prince, Alfred M., 626Probst, Peter G., 402Pursell, Alfred R., 537

Qadri, S. M. Hussain, 102

Reinhardt, George F., 62Richman, Douglas D., 223Riddle, Conradine F., 206Roberts, Glenn D., 47, 157, 302Robertson, E. Arthur, 421Robinson, David M., 460Robrish, S. A., 474Rocha, Heonir, 67Rodr;guez, A., 26Rolfe, Rial D., 218Rottem, Shlomo, 110Rowen, Joyce W., 137Rubin, Sally J., 582Rudback, J. A., 21Ruitenberg, E. J., 541Russell, H., 501

Sack, David A., 486

Sack, R. Bradley, 486Sammons, L. S., 513Samuels, Sandra, 49Sandok, P. L., 72Sanford, G., 496Saphir, D. A., 344Saunders, George C., 604Savy, V. L., 26Saz, Arthur K., 330Schlossberg, David, 239Schneider, Lothar G., 96Schreckenberger, Paul C., 313Schryver, George D., 397Schwarz, Jan, 175Sechter, I., 390Segal, Herbert E., 465Selin, Merle J., 180Selva-Sutter, Ernesta A., 414Shepard, Maurice C., 613Shulman, Jonas A., 234Sielaff, Bruce H., 105Silcox, Vella A., 414Sivendra, R., 70Smart, Ross A., 376Smibert, R. M., 432Smith, John A., 5Smith, Rodney F., 640Smith, Thomas F., 334Sniderman, Steve, 77Socransky, S. S., 474Spendlove, Rex S., 376Staat, Robert H., 378Stalons, Donald R., 161Standard, Paul G., 191Stargel, M. D., 206, 291Steerenberg, P. A., 541Stevens, Robert J., 576Stotzky, Guenther, 119Strannegard, Oxian, 86Stretton, R. J., 635Sullivan-Sigler, Nadine, 133Sultz, Harry A., 469Sun, S. H., 186Syed, Salam A., 200

Taber, Larry H., 309Talbot, Henry W., Jr., 519Taylor, Welton I., 206Thirumoorthi, M. C., 318Thomas, K. R., 21Thompson, F. S., 291Thornsberry, C., 425Tiku, Moti L., 469Top, Franklin H., Jr., 465Treadwell, Thomas L., 287Triantafellu, N., 593Tu, Kevin K., 77Tzianabos, T., 609

Uhlendorf, Carol, 223

Valu, Joseph A., 172Vasil, Michael L., 382Vnek, John, 626Vukovich, K. R., 186

AUTHOR INDEX iii

Wang, Howard S., 302Wannamaker, Lewis W., 533Warren, Edward, 212Washington, John A., II, 212,

643Weaver, R. E., 425Weetall, Howard H., 402

Weissman, Jack B., 143Wells, Joy G., 486Wende, R. D., 102West, Susan E. H., 393Wheat, Robert, 545White, L. A., 609Wilkins, Tracy D., 359, 393

Wilkinson, Hazel W., 480Willhight, Milton, 465Williams, D. N., 556Williams, R. P., 102Wong, James, 640Wooley, R. E., 149

SUBJECT INDEX

VOLUME 3

Acetylmethylcarbinolproduced by N. gonorrhoeae, 34

Acute central nervous system infectionsviral antibody in, 397

Aerobic bacteriaschemata for identification, 161

Aeromonas hydrophilameningitis, 102

Affinity chromatographyelimination of bacteriophages

by, 402Agglutination test

false positive histoplasmin latex, 306serogrouping ofB. fragilis

subsp. fragilis, 586Anaerobes

survival in clinical specimens, 133Anaerobic bacteria

evaluation of schemata for detection of, 161in nonsupportive gassed transport system, 128modification of Minitek system to characterize,

291rapid fermentation testing of, 313

Anaerobic cell culture systemT. pallidum, 72

Antibodiesbronchitis virus, 149

Antigenhepatitis B surface, 626T, 593

API 20 C microtube systemto identify yeasts, 302

API 20S and API 20E kitsto identify Enterobacteriaceae, 421

Arcton 113to purify influenza virus, 524

ASO/ADB test kitevaluation of, 458

Autobac 1bacterial identification, 105

Bacillimethod for identification, 566

Bacteremiaeffect of volume of blood on, 643radiometric detection, 281

Bacteriaanaerobic, 161anaerobic, aerobic, 128effected by sonic treatment, 474tetrazolium reduction by, 327

Bacterial identificationcomputer-assisted, 105

Bacteriuriaassociation with bacteremia, 246

Bacteroides corrodensisolates of anaerobic gram-negative rods, 432

Bacteroides fragilisinhibition on blood agar plates, 359

Bacteroides fragilis subsp. fragilis

serogrouping by agglutination, 586Bacteroides melaninogenicus

description by fluorescent antibody staining, 506Bacti-Lab streptococci culture systems

to identify group A streptococci, 443Binary ethyleneimine

inactivation of viruses in serum with, 209Blood agar plates

inhibition of B. fragilis on, 359Blood cultures

Vibrio sp. isolated from, 425Blood culture technique

clinical evaluation, 251developmental phase, 258

Bronchitis virus antibodiesmicroneutralization test, 149

Brucella abortus antibodiesidentification of, 203

Cancermicrobial skin flora, 14

Candida agglutinin testin diagnosis of candidiasis, 175

Candidiasistests for diagnosis, 175

Catalase-negative strainS. aureus, 77

Ceratocystiscross-reactions with S. schenckii, 545

Cetylpyridinium chlorideeffect on fungi and N. asteroides in sputum, 272

Chagas' diseasedetection of antibodies, 67

Childrenanaerobic infections in, 318

Chlamydia subgroup Acomparison after incubation, 334

Choleracaused by enterotoxigenic E. coli, 382

Chromatographic detectionelectron capture gas, 34

Citrobacter diversusisolated from clinical material, 390

Clostridium chauvoeielectron capture gas chromatography study, 180

Clostridium septicumelectron capture gas chromatography study, 180

Coccidioides immitisidentification of, 186

Complement fixation testcompared with counterimmunoelectrophoresis

test, 67for histoplasmosis, 157

Conjugatesfluorescent antibody, 2

Coronavirusdetection by fluorescent virus precipitin test, 376

Cotton swab and scrub methodsfor differential quantitation of bacteria, 576

Counterimmunoelectrophoresisiv

SUBJECT INDEX v

compared with complement fixation test, 67of staphylococcal antibody, 599to recognize group B streptococci, 287

Cytomegalovirusdetection of antibodies, 364

Decarboxylasecompared with dihydrolase results, 75for differentiation of bacteria, 137

Dental plaque floramedium to detect gelatin-hydrolyzing activity,

200Diarrheamethods to detect E. coli, 486

Diarrhea virusinfant reovirus-like, 376neonatal calf, 376

Differential agar medium (A7)to identify U. urealyticum, 613

Dihydrolasecompared with decarboxylase results, 75for differentiation of bacteria, 137

Duodenal isolationof S. typhi, 456

Electron capture gas chromatography studyof C. septicum and C. chauvoei, 180

EnterobacteriaceaeAPI 20E and API 20S kits, 421unusual, 206

Enzyme-labeled antibody test, 604Equine encephalomyelitis viruses

preparation and testing of vaccines, 113Escherichia coli

as cause of clinical cholera, 382methods to detect, 486

Ethyleneimineviruses with, 209

Eubacterium lentumcontamination with S. sonnei, 206

Fluorescein-125I antibodyto detect virus-specific immunoglobulins, 637

Fluorescent antibody conjugatesstability of, 1

Fluorescent antibody stainingofB. melaninogenicus, 506of virus-infected cell cultures, 537

Fluorescent antibody testto diagnose gonorrhea, 438

Fluorescent virus precipitin testdetection of viruses, 375

Formate-fumerate energy metabolismB. corrodens, V. succinogenes, 432

Fungirecovery from clinical specimens, 47

Gas chromatographic characterizationof mycobacteria, 81

Gas chromatographyto detect cryptococcal meningitis, 239

Gelatin-hydrolyzing activityof human dental plaque flora, 200

Gentamicinclinical evaluation, 496

Germ tube dispersion testfor diagnosis of candidiasis, 175

Gingival florabacteria associated with dog bites, 344

Gloved-hand modelfor measurements of skin-degerming activity, 406

Gonorrheafluorescent antibody test, 438

Group B streptococcal typesIa, Tb, Ic, II, and III, 480

Group B streptococcirecognition by pigment production and counter-

immunoelectrophoresis, 287Gum tragacanth overlay

in plaque assay of viruses, 373

hardjo, from horses of Argentina, 548Hemadsorption inhibition method

filtration of Newcastle disease virus in, 227Hemolysis-in-gel test

for screening of rubella immunity, 86Hepatitis B surface antigen

in health care institution, 469persistence of antibody to, 465purification of, 626

Herpes simplex virus infectiondiagnosis by immunofluorescence, 309

Herpesvirus hominisuse of biological characteristics, 277

Hippurate hydrolysisby group B Streptococcus, 49

Histoplasmatest for identification of, 191

Histoplasmosiscomplement fixation tests, 157

Human diploid cell culturevaccine, 96

Immune globulinrabies fluorescein isothiocyanate-labeled, 609

Immunodiffusion testinfluenza virus A neuraminidase antigens, 233

Immunofluorescencediagnosis of herpes simplex virus infection, 309

Immunofluorescent identificationof mycoplasma colonies, 449

Immunological testto identify Histoplasma, 191

Immunoperoxidase techniqueto detect antibodies to cytomegalovirus, 364

Immunosorbent assayfor streptococcal M protein antibodies, 501measurement of rucroplites, 541

Indirect enzyme-labeled antibody conceptscreening for antibodies, 604

InfectionsShigella, 143

Influenza A virusinterferon sensitivity, 223neuraminidase antigens, 233

Influenza viruspurification by Arcton 113, 524

Isolation of anaerobeseffectiveness of palladium chloride for, 218

Leifson flagella strain

vi SUBJECT INDEX

simplified, 632Leptospira biflexa, 548

isolation from horses, 548Long-chain fatty acids

of Sporothrix (Sporotrichum) schenckii, 632

McCoy's cells5-iodo-2-deoxyuridine-treated, 334

Mediumfor detecting dental plaque flora, 200

Meningitisdiagnosis by gas chromatography, 239due to A. hydrophila, 102

Methanolysatestrifluoracetylated whole-cell, 81

Methodscotton swab and scrub, 576modified hemadsorption, 227Moeller, 75rapid, 75, 566replicator plate, 75simple disk-plate, 172

Microbial skin florafrom cancer patients, 14

Micrococcaceaecoagulase-negative, 556

Microimmunofluorescence testfor study of Rocky Mountain spotted fever, ty-

phus, 51Microneutralization test

to determine bronchitis virus antibodies, 149Microrespirator chamber

to determine viability, 560Microtiter test

cholesterol requirement of mycoplasmas, 110Minitek system

for characterization of anaerobic bacteria, 291to identify N. gonorrhoeae, 8

Mitis-Salivarius agarinhibition of S. mutans, 378

Modified hemadsorption methodtitration of Newcastle disease virus, 227

Mycobacteriagas chromatographic characterization of, 81

Mycobacteriological culturerecovery of fungi, 47

Mycobacteriumresembling M. fortuitum, 453

Mycobacterium fortuitumthat produces brown pigment, 453use of phage F-4WJ-1, 324

Mycobacterium gordonaesimple procedure for detection, 211

Mycobacterium szulgaidifferential identification of, 414

Mycobacterium tuberculosisdiscerning more phage types, 324

Mycological mediawith gentamicin added, 496

Mycoplasma coloniesimmunofluorescent identification, 449

Mycoplasmascholesterol requirement of, 110

Neisseriasimple disk-plate method, 172

Neisseria gonorrhoeaeacetylmethylcarbinol produced by, 34identification with Minitek system, 8production of acid from glucose, 330

Neutralization testinfluenza virus A neuraminidase antigens, 233

Newcastle disease virusand its neutralizing antibodies, 227

Nocardia sp.use of C02, 463

Nocardia asteroideseffect of cetylpyridinium chloride on, 272

Nonsupportive gassed transport systemanaerobic and aerobic bacteria, 128

Palladium chlorideeffectiveness of, 218

Peptostreptococcus anaerobiusinhibition of, 393

Phage typingof S. epidermidis, 519

Phage typing setfor salmonellae, 214

Plaque assayof Sendai virus, 71

Precipitin testfor diagnose Candidiasis, 175

Procedureto detect M. gordonae, 211

Prophylactic immunizationof humans against rabies, 96

Proteus rettgeri 0-serotyping systemevaluation of, 385

Pseudomonas aerginosaepidemiology of infections, 264

Pseudomonas cepaciafrom water reservoirs, 62

Pseudomonas stutzeripitting of agar surface by, 381

Pyocen typingdetermining P. aerginosa infections, 264

Rabiesfluorescein isothiocyanate, 609prophylactic immunization, 96

Radioimmunoassayfor measuring group B streptococcal types, 480

Radiometric detectionof bacteremia, 281

Rapid decarboxylase testfor differentiation of bacteria, 137

Rapid fermentation testingof anaerobic bacteria, 313

Rhamnose-positive Y. enterocoliticabiochemical characteristics, 119

Rheumatoid factorblocking effect, 157

Rhinovirusisolation of, 528

Rocky Mountain spotted feverantibody response to, 513microimmunofluorescence test, 51

Rubella hemagglutination inhibitionevaluation of, 5

Salmonella

method for identifying, 339Salmonellaegroup Cl and C2, 214

Salmonella typhiduodenal isolation of, 456

Screening of rubella immunityhemolysis-in-gel-test, 86

Sendai virusplaque assay, 91

Serotype hardjofrom horses, 548

Serratia marcescens

serotyping and biotyping of, 582Shigellamethod for identifying, 339

Shigella infectionsseroepidemiology of, 143

Shigella sonneicontamination with E. lentum, 206

Simian virus 40-induced T-antigen expression, 593

Simple disk plate methodfor confirmation of Neisseria, 172

Single radial diffusionto identify B. abortus, 203

Skin-degerming activitygloved-hand model, 406

Sodium amylosulfatecompared with sodium polyanetholsulfonate, 212

Sodium polyanetholsulfonatecompared with sodium amylsulfate, 212P. anaerobius inhibition by, 393

Sonic treatmenteffect on bacteria, 474

Sporothrix schenckiicross-reactions with Ceratocystis, 545

Sporothrix (Sporotrichum) schenckiilong-chain fatty acids of, 635

Sporotrichotic patientsdelayed cross-reactions in, 545

Staphylococcal antibodycounterimmunoelectrophoresis of, 599

Staphylococcus aureus

catalase-negative strain, 77Staphylococcus epidermidisphage typing of, 519

Streptococcal M antibodiesmeasured by radioimmunoassay, 501

StreptococciBacti-Lab streptococci culture systems, 443

Streptococcus (group B)determination of Hippurate hydrolysis, 49

Streptococcus mutansinhibition by Mitis-Salivarius agar, 378

Streptococcus pyogenesnew method to detect M types of, 533

Techniqueblood culture, 251, 258immunoperoxidase, 364

Testagglutin, 175

SUBJECT INDEX vii

agglutination, 586complement fixation, 67counterimmunoelectrophoresis, 67decarboxylase, 75, 137dihydrolase, 75, 137enzyme-labeled antibody, 604fluorescent antibody, 438germ tube dispersion, 175hemolysis-in-gel, 86immunodiffusion, 227microimmunofluorescence, 51microtiter, 110neutralization, 233precipitin, 175rubella hemagglutination inhibition, 5to determine Hippurate hydrolysis, 49

Tetrazolium reductionby bacteria, 327

Treponema pallidumin an aerobic cell culture system, 72

Tuberculosisfalse positive histoplasmin latex agglutination

test, 306Typhusmicroimmunofluorescence test, 51

Ureaplasma urealyticumdifferential agar medium (A7) to identify, 613

Vaccinehuman diploid cell culture, 96prepared from equine encephalomyelitis viruses,

113Venezuelan equine encephalomyelitis, 460

Vaginal yeastsisolation on media, 640

Venezuelan equine encephalomyelitis vaccineimproved method for production of, 460

Vibriohalophilic, 425

Vibrio succinogenesisolates of anaerobic gram-negative rods, 432

Viral antibodyin acute central nervous system infections, 397

Virushuman cytomegalovirus, 364influenza A, 223Newcastle disease, 227Sendai, 91

Viruses in plaque assayuse of gum tragacanth overlay, 373

Virus neutralization testto determine bronchitis virus antibodies, 147

Virus-specific immunoglobulinsdetection of, 637

Yeastsidentified by API 20C microtube system, 302vaginal, 640

Yersinia enterocoliticarhamnose-positive, 119

INDEX TO DATE OF ISSUE

Month Date of Issue Pages

January 6 February 1976 1-80February 2 March 1976 81-221March 24 March 1976 223-384April 30 April 1976 385-464May 25 May 1976 465-543June 2 July 1976 545-646

JOURNALOF

MICROBIOLOGY

VOLUME 3

WASHINGTON, D.C. 20006

1976

JOURNAL OF CLINICAL MICROBIOLOGYVOLUME 3 1976

EDITORIAL BOARDALBERT BALOWS, Editor-in-Chief (1979)

Center for Disease Control,Atlanta, Ga.

LORRAINE FRIEDMAN, Editor (1979)Tulane University Medical School,

New Orleans, La.

HENRY D. ISENBERG, Editor (1979)Long Island Jewish-Hillside Medical Center,

New Hyde Park, N. Y.NATHALIE J. SCHMIDT, Editor (1980)

California Department of Health,Berkeley, Calif

Daniel Amsterdam (1977)Libero Ajello (1976)William B. Cherry (1978)Walter R. Dowdle (1976)V. R. Dowell, Jr. (1977)Richard W. Emmons (1977)John C. Feeley (1917)James D. Fenters (1976)Sydney M. Finegold (1976)Maxwell Finland (1977)Herman Friedman (1978)Thomas L. Gavan (1977)Morris A. Gordon (1978)Dieter Groschel (1977)Leonor D. Haley (1977)James L. Hardy (1978)Melvin T. Hatch (1978)Harry R. Hill (1977)

Lillian V. Holdeman (1976)Milton Huppert (1978)S. S. Kalter (1978)Leo Kaufman (1977)George E. Kenny (1976)George P. Kubica (1976)Howard W. Larsh (1977)James D. MacLowry (1977)Harold Markowitz (1977)William J. Martin (1977)John M. Matsen (1977)Joseph L. Melnick (1976)William I. Metzger (1977)Maurice A. Mufson (1977)Barbara G. Painter (1977)Demosthenes Pappagianis (1977)Robert H. Purcell (1977)William E. Rawls (1977)

Glenn D. Roberts (1977)Jon E. Rosenblatt (1977)Margarita Silva-Hutner (1977)James W. Smith (1977)Louis DS. Smith (1978)Peter B. Smith (1976)Alex C. Sonnenwirth (1977)Welton I. Taylor (1977)John H. Thompson, Jr. (1977)Richard C. Tilton (1978)Marvin Turck (1977)Alexander von Graevenitz (1977)Kenneth W. Walls (1977)Lewis W. Wannamaker (1978)Robert E. Weaver (1978)Irene Weitzman (1978)Robert P. Williams (1978)Marion E. Wilson (1977)

Robert A. Day, Managing EditorGisella Pollock, Director of Editorial Services

Leslie Frymire, Production Editor1913 I St., N. W., Washington, D.C. 20006

EX OFFICIOHelen R. Whiteley, President (1975-1976) Harlyn 0. Halvorson, Vice-President (1975-1976)

J. Mehsen Joseph, Secretary Brinton M. Miller, Treasurer

The Journal of Clinical Microbiology, a publication of theAmerican Society for Microbiology, 1913 I St., N. W., Wash-ington, D.C. 20006, is devoted to the dissemination of newknowledge concerning the applied microbiological aspects ofhuman and animal infections and infestations, particularlyregarding their etiologic agents, diagnosis, and epidemiology.Papers dealing with antibiotics and antimicrobial agents andchemotherapy, with fundamental aspects of infection andimmunity, and with food or dairy microbiology fall within thescope of other ASM publications. The Journal is publishedmonthly, and the twelve numbers are divided into two volumesper year. The nonmember subscription price is $50 per year.The member subscription price is $13 per year. Single copiesare $5. Correspondence relating to subscription, reprints,

defective copies, availability of back issues, lost or late proofs,disposition of submitted manuscripts, and general editorialmatters should be directed to the ASM Publications Office,1913 I St., N.W., Washington, D.C. 20006 (area 202833-9680).

Second-class postage paid at Washington, D.C. 20006, and atadditional mailing offices.Made in the United States of America.Copyright © 1976, American Society for Microbiology.1976, American Society for Microbiology.All Rights Reserved.

Volume 3 Contents for January Number I

BacteriologyAdaptation of the Minitek System for the Rapid Identification of Neisseria

gonorrhoeae. STEPHEN A. MORSE* AND LYNNE BARTENSTEIN .... .......... 8Electron Capture Gas Chromatographic Detection of Acetylmethylcarbinol Pro-

duced by Neisseria gonorrhoeae. C. DWAYNE MORSE, JOHN B. BROOKS,* ANDDOUGLAS S. KELLOGG, JR . .............................................. 34

Evaluation of an Adenosine 5'-Triphosphate Assay as a Screening Method toDetect Significant Bacteriuria. DAVID N. ALEXANDER, GRACE M. EDERER,AND JOHN M. MATSEN* ............. .................................... 42

Rapid, Colorimetric Test for the Determination of Hippurate Hydrolysis by GroupB Streptococcus. STEPHEN C. EDBERG* AND SANDRA SAMUELS .... ........ 49

Unusual Chromobacterium violaceum: Aerogenic Strains. R. SIVENDRA ....... 70Retention of Motility of Treponema pallidum (Nichols Virulent Strain) in an An-

aerobic Cell Culture System and in a Cell-Free System. P. L. SANDOK,*H. M. JENKIN, S. R. GRAVES, AND S. T. KNIGHT .......................... 72

Comparison of Amino Acid Decarboxylase and Dihydrolase Results by Moeller,Rapid, and Replicator Plate Methods. RONALD N. JONES,* PETER C.FUCHS, AND STEVE SNIDERMAN .......................................... 75

Isolation and Characterization of a Catalase-Negative Strain of Staphylococcusaureus. KEVIN K. Tu* AND WALDEMAR A. PALUTKE ..... ................ 77

Virology, Rickettsiology, and ChlamydiologyEvaluation of a Rubella Hemagglutination Inhibition Test System.

JOHN A. SMITH* AND A. C. CUMMINS .................................... 5

Ethylenimine-Inactivated Rabies Vaccine of Tissue Culture Origin. 0. P.LARGHI, V. L. SAVY, A. E. NEBEL, AND A. RODRIGUEZ* ..... .............. 26

Microimmunofluorescence Test for the Serological Study of Rocky MountainSpotted Fever and Typhus. R. N. PHILIP,* E. A. CASPER, R. A. ORMSBEE,M. G. PEACOCK, AND W. BURGDORFER ................................... 51

MycologyRecovery of Pathogenic Fungi from Clinical Specimens Submitted for Myco-

bacteriological Culture. GLENN D. ROBERTS,* ALFRED G. KARLSON, ANDDONALD R. DEYOUNG .............. .................................... 47

Nosocomial InfectionsMicrobial Skin Flora of Selected Cancer Patients and Hospital Personnel.

MOLLIE E. McBRIDE,* W. CHRISTOPHER DUNCAN, GERALD P. BODEY, ANDCHARLES M. MCBRIDE .................................................. 14

Pseudomonas cepacia Strains Isolated from Water Reservoirs of Unheated Nebu-lizers. SHELDON M. GELBART,* GEORGE F. REINHARDT, AND HERBERT B.GREENLEE ............................................................. 62

ParasitologyComparison of the Complement Fixation Test and Counterelectrophoresis Test

for the Detection of Antibodies in Chagas Disease. RALPH A. KNIGHT,*HEONIR ROCHA, AND DONALD KAYE ........ .............................. 67

Clinical Laboratory ImmunologyStability of Fluorescent Antibody Conjugates Stored Under Various Conditions.* Asterisk refers to person to whom inquiries regarding the paper should be addressed.

xi

JAMES H. GREEN, SAMUEL B. GRAY, JR., AND W. KNOX HARRELL* .1Preparation and Properties of a National Reference Endotoxin. J. A. RuD-

BACH,* F. I. AKIYA, R. J. ELIN, H. D. HOCHSTEIN, M. K. LUOMA, E. C. B.MILNER, K. C. MILNER, AND K. R. THOMAS .............................. 21

ERRATUMIndirect Hemagglutination Test for Human Antibody to Typhus and Spotted

Fever Group Rickettsiae. AKIRA SHIRAI, JOHN W. DIETEL, AND JOSEPHV. OSTERMAN*........................................................ 79

Volume 3 Contents for February Number 2Bacteriology

Gas Chromatographic Characterization of Mycobacteria: Analysis of Fatty Acidsand Trifluoroacetylated Whole-Cell Methanolysates. LENNART LARSSON*AND PER-ANDERS MARDH ........... .................................... 81

Meningitis Due to Aeromonas hydrophila. S. M. HUSSAIN QADRI,* L. P. GORDON,R. D. WENDE, AND R. P. WILLIAMS ........ .............................. 102

Computer-Assisted Bacterial Identification Utilizing Antimicrobial SusceptibilityProfiles Generated by Autobac 1. BRUCE H. SIELAFF,* EUGENE A. JOHNSON,AND JOHN M. MATSEN ............. .................................... 105

Temperature-Dependent Cultural and Biochemical Characteristics of Rhamnose-Positive Yersinia enterocolitica. BRENT CHESTER* AND GUENTHER STOTZKY . 119

Survival of Anaerobic and Aerobic Bacteria in a Nonsupportive Gassed TransportSystem. ANTHONY W. CHOW,* PATRICK J. CUNNINGHAM, AND LUCIEN B.GUZE ................................................................. 128

Anaerobes Survive in Clinical Specimens Despite Delayed Processing. JOHN G.BARTLETT,* NADINE SULLIVAN-SIGLER, THOMAS J. LOUIE, AND SHERWOOD L.GORBACH ..............................................................133

Evaluation ofthe Rapid Decarboxylase and Dihydrolase Test for the Differentiationof Nonfermentative Bacteria. THOMAS R. OBERHOFER,* JOYCE W. ROWEN,JAMES W. HIGBEE, AND RONALD W. JOHNS ...... ........................ 137

Evaluation of Simplified Dichotomous Schemata for the Identification of AnaerobicBacteria from Clinical Material. RICHARD K. PORSCHEN* AND DONALD R.STALONS .............................................................. 161

Simple Disk-Plate Method for the Biochemical Confirmation of Pathogenic Neis-seria. JOSEPH A. VALU ............ .................................... 172

Electron Capture Gas Chromatography Study of the Acid and Alcohol Products ofClostridium septicum and Clostridium chauvoei. JOHN B. BROOKS,* MERLE J.SELIN, AND CYNTHIA C. ALLEY ......... ................................. 180

A New Medium for the Detection of Gelatin-Hydrolyzing Activity of Human Den-tal Plaque Flora. SALAM A. SYED ........ .............................. 200

Unusual Enterobacteriaceae: H2S+ Shigella sonnei, One Authentic and One FalsePositive Due to Contamination with the Obligate Anaerobe Eubacteriumlentum. J. J. FARMER III,* CONRADINE F. RIDDLE, M. D. STARGEL, H. IIDA,T. AIKAWA, DAVID ACHANZAR, AND WELTON I. TAYLOR ...... .............. 206

Simple Procedure for Detection of Mycobacterium gordonae in Water CausingFalse-Positive Acid-Fast Smears. DELIA DIZON, CORNELIA MIHAILESCU, ANDH. C. BAE* ........................................................... 211

Comparison of Sodium Amylosulfate and Sodium Polyanetholsulfonate in Blood

xii CONTENTS

Culture Media. M. MARSHA HALL, EDWARD WARREN, DUANE M. ILSTRUP,AND JOHN A. WASHINGTON II* ......... ................................. 212

Effectiveness of Palladium Chloride for the Isolation of Anaerobes. DWIGHT R.OWENS,* RIAL D. ROLFE, AND DAVID J. HENTGES ...... ................... 218

Virology, Rickettsiology, and ChlamydiologyEvaluation of the Hemolysis-in-Gel Test for the Screening of Rubella Immunity

and the Demonstration of Recent Infection. LENA GRILLNER* AND ORJANSTRANNEGARD......................................................... 86

Plaque Assay of Sendai Virus in Monolayers of a Clonal Line of Porcine KidneyCells. H6MU ITO...................................................... 91

Prophylactic Immunization ofHumans Against Rabies by Intradermal Inoculationof Human Diploid Cell Culture Vaccine. JAMES H. Cox* AND LOTHAR G.SCHNEIDER ............................................................. 96

Preparation and Testing of Vaccines Prepared from the Envelopes of Venezuelan,Eastern, and Western Equine Encephalomyelitis Viruses. CARL E. PEDER-SEN, JR . .............................................................. 113

Comparison ofa Microneutralization Test in Cell Culture and Virus NeutralizationTest in Embryonated Eggs for Determining Infectious Bronchitis Virus Anti-bodies. R. E. WOOLEY,* J. BROWN, R. B. DAVIS, J. L. BLUE, AND P. D.LUKERT ............................................................... 149

Inactivation of Viruses in Serum with Binary Ethyleneimine. HANs G. BAHNE-MANN ................................................................ 209

MycologyBlocking Effect of Rheumatoid Factor and Cold Agglutinins on Complement Fixa-

tion Tests for Histoplasmosis. JAMES E. JOHNSON AND GLENN D. ROBERTS . 157Comparative Evaluation of the Candida Agglutinin Test, Precipitin Test, and

Germ Tube Dispersion Test in the Diagnosis of Candidiasis. DONNA OBLACK,JAN SCHWARZ, AND IAN A. HOLDER* ..................................... 175

Rapid In Vitro Conversion and Identification of Coccidioides immitis. S. H. SUN,M. HUPPERT,* AND K. R. VUKOVICH ..................................... 186

Specific Immunological Test for the Rapid Identification of Members of the GenusHistoplasma. PAUL G. STANDARD* AND LEO KAUFMAN ..... .............. 191

ImmunologyIdentification ofBrucella abortus Antibodies in Cattle Serum by Single Radial Dif-

fusion. DOMENICO IANNELLI,* RAMON DIAZ, AND T. MANLIO BETTINI ...... 203

EpidemiologyDiagnostic Value of Indirect Hemagglutination in the Seroepidemiology ofShigella

Infections. CHARLOTTE M. PArrON,* EUGENE J. GANGAROSA, JACK B. WEISS-MAN, MICHAEL H. MERSON, AND GEORGE K. MORRIS ..... ................. 142

Phage Typing Set for Group Cl and C2 Salmonellae. M. GERSHMAN .... ....... 214

MycoplasmatologyCholesterol Requirement of Mycoplasmas as Determined by a Microtiter Test

Using Polyene Antibiotics. MARION W. GRABOWSKI,* SHLOMO ROTTEM, ANDMICHAEL F. BERILE..................................................... 110

ERRATAPolypectate Digestion by Yersinia. V. LYLE VON RIESEN ..... ................ 221Convenient, Simplified Preparation of Less Commonly Used Media. V. LYLE

VON RIESEN ........................................................... 221

CONTENTS *x.ii

Improved Transport System for Neisseria gonorrhoeae in Clinical Specimens.DOROTHY A. SYMINGTON................................................. 1

Volume 3 Contents for March Number3Bacteriology

Polymicrobic Bacteriuria: Significant Association with Bacteremia. PETER A.GROSS,* MARGARET FLOWER, AND GERTRUDE BARDEN ...................... 246

Blood Culture Technique Based on Centrifugation: Developmental Phase. GOR-DON L. DORN,* JOHN R. HAYNES AND G. GENE BURSON .................... 251

Blood Culture Technique Based on Centrifugation: Clinical Evaluation. GORDONL. DORN,* G. GENE BURSON, AND JOHN R. HAYNES....................... 258

Clinical Comparison of Aerobic, Hypertonic, and Anaerobic Culture Media for theRadiometric Detection of Bacteremia. R. MARIE COLEMAN, W. WAYNELASLIE, AND D. W. LAMBE, JR.*...... ................................... 281

Rapid Recognition of Group B Streptococci by Pigment Production and Counter-immunoelectrophoresis. KATHARINE MERRITT,* THOMAS L. TREADWELL, ANDNICHOLAS J. JACOBS................................................... 287

Modification of the Minitek Miniaturized Differentiation System for Characteriza-tion of Anaerobic Bacteria. M. D. STARGEL,* F. S. THOMPSON, S. E. PHILLIPS,G. L. LOMBARD, AND V. R. DOWELL, JR ................................. 291

Rapid Fermentation Testing of Anaerobic Bacteria. PAUL C. SCHRECKENBERGER*AND DONNA J. BLAZEVIC............................................... 313

Anaerobic Infections in Children: a Prospective Survey. M. C. THIRUMOORTHI,BARBARA M. KEEN, AND ADNAN S. DAJANI .............................. 318

Use of Phage F-OWJ-1 of Mycobacterium fortuitum to Discern More Phage Typesof Mycobacterium tuberculosis. W. D. JONES, JR.,* AND J. GREENBERG..... 324

Acceleration of Tetrazolium Reduction by Bacteria. RAYMOND C. BARTLETT,MARY MAZENS,* AND BRIAN GREENFIELD ................................. 327

Effect of Types of Media on the Production of Acid from Glucose by So-CalledGlucose-Negative Strains of Neisseria gonorrhoeae. ELLEN S. BARON ANDARTHUR K. SAZ*....................................................... 330

Method for Identifying Salmonella and Shigella Directly from the Primary Isola-tion Plate by Coagglutination of Protein A-Containing Staphylococci Sen-sitized with Specific Antibody. EARL A. EDWARDS* AND RICHARD L.HILDERBRAND.......................................................... 339

Gingival Flora of the Dog with Special Reference to Bacteria Associated withBites. D. A. SAPHIR AND G. R. CARTER* ................................ 344

Differentiation of Proteus mirabilis by Bacteriophage Typing and the Dienes Re-action. F. W. HICKMAN AND J. J. FARMER III* ..... ..................... 350

Inhibition of Bacteroides fragilis on Blood Agar Plates and Reversal of Inhibitionby Added Hemin. TRACY D. WILKINS,* SARAH L. CHALGREN, F. JIMENEZ-ULATE, C. R. DRAKE, JR., AND J. L. JOHNSON ..... ....................... 359

Inhibition of Streptococcus mutans Strains by Different Mitis-Salivarius AgarPreparations. ROBERT H. STAAT ........ ............................... 378

Pitting of Agar Surface by Pseudomonas stutzeri. JANE J. ALEXANDER AND JAYF. LEWIS* ............................................................ 381

Clinical Cholera Caused by Enterotoxigenic Escherichia coli. RICHARD A.FINKELSTEIN,* MICHAEL L. VASIL, JAMES R. JONES, RICHARD A. ANDERSON,AND TOM BARNARD..................................................... 382

XIV CONTENTS

Virology, Rickettsiology, and ChlamydiologyThree Strains of Influenza A Virus (H3N2): Interferon Sensitivity In Vitro and

Interferon Production in Volunteers. DOUGLAS D. RICHMAN, BRIAN R. MUR-PHY,* SAMUEL BARON, AND CAROL UHLENDORF ...... ..................... 223

Titration of Newcastle Disease Virus and Its Neutralizing Antibodies in Micro-plates by a Modified Hemadsorption and Hemadsorption Inhibition Method.DAVID KATZ,* HANA BEN-MOSHE, AND SHLOMIT ALON ..... ................ 227

Antigenic Relationships Among Influenza Virus A Neuraminidase (N2) Antigensby Immunodiffusion and Postinfection Neutralization Test. WALTER R.DOWDLE,* W. GRAEME LAVER, JUDITH C. GALPHIN, AND JEAN C. DOWNIE .. 233

Use of Biological Characteristics to Type Herpesvirus hominis Types 1 and 2 inDiagnostic Laboratories. SHERRY MARKS-HELLMAN AND MONTO Ho* ...... 277

Diagnosis of Herpes Simplex Virus Infection by Immunofluorescence. LARRYH. TABER,* FRANCOISE BRASIER, ROBERT B. COUCH, STEPHEN B. GREENBERG,DAN JONES, AND VERNON KNIGHT ........ ............................... 309

Comparison of Chlamydia Subgroup A Detection from Clinical Specimens After40 and 64 Hours of Incubation in 5-Iodo-2-Deoxyuridine-Treated McCoy'sCells. JAMES E. JOHNSON* AND THOMAS F. SMITH ..... .................. 334

Immunoperoxidase Technique for Detection of Antibodies to Human Cytomegalo-virus. G. GERNA,* C. J. MCCLOUD, AND R. W. CHAMBERS ..... ........... 364

Use of Gum Tragacanth Overlay, Applied at Room Temperature, in the PlaqueAssay of Fish and Other Animal Viruses. PETER DOBOS ..... ............ 373

Detection of Neonatal Calf Diarrhea Virus, Infant Reovirus-Like Diarrhea Virus,and a Coronavirus Using the Fluorescent Virus Precipitin Test. MARTINW. PETERSON, REX S. SPENDLOVE,* AND Ross A. SMART ..... .............. 376

MycologyPossibility of Diagnosing Meningitis by Gas Chromatography: Cryptococcal

Meningitis. DAVID SCHLOSSBERG, JOHN B. BROOKS,* AND JONAS A. SHULMAN 239

Effect of Cetylpyridinium Chloride on Pathogenic Fungi and Nocardia asteroidesin Sputum. BETTY J. PHILLIPS* AND WILLIAM KAPLAN ..... .............. 272

Evaluation of the API 20 C Microtube System for the Identification of ClinicallyImportant Yeasts. GLENN D. ROBERTS,* HOWARD S. WANG, AND GARY E.HOLLICK .............................................................. 302

Apparent False Positive Histoplasmin Latex Agglutination Test in Patients withTuberculosis. ARTHUR F. DISALVO* AND DOROTHY S. CORBETT .... ........ 306

EpidemiologyEpidemiology of Pseudomonas aeruginosa Infections: Determination by Pyocin

Typing. JOHAN N. BRUUN, GERARD J. MCGARRITY,* WILLIAM S. BLAKEMORE,AND LEWIS L. CORIELL ............. .................................... 264

Volume 3 Contents for April Number 4Bacteriology

Citrobacter diversus Isolated from Clinical Material. G. ALTMANN, I. SECHTER,*D. CAHAN, AND CH. B. GERICHTER ...................................... 390

Medium-Dependent Inhibition of Peptostreptococcus anaerobius by Sodium Poly-anetholsulfonate in Blood Culture Media. TRACY D. WILKINS* AND SUSAN E.H. WEST .............................................................. 393

Differential Identification of Mycobacterium szulgai and Other Scotochromogenic

CONTENTS xv

Mycobacteria. ERNESTA A. SELVA-SUTTER, VELLA A. SILCOX,* AND HUGO L.DAVID ................................................................ 414

Analysis of Cost and Accuracy of Alternative Strategies for EnterobacteriaceaeIdentification. E. ARTHUR ROBERTSON,* GERALD C. MACKS, AND JAMES D.MACLOWRY ............................................................ 421

Halophilic Vibrio Species Isolated From Blood Cultures. D. G. HOLLIS,* R. E.WEAVER, C. N. BAKER, AND C. THORNSBERRY ............................ 425

Clinical Isolates of Anaerobic Gram-Negative Rods with a Formate-FumerateEnergy Metabolism: Bacteroides corrodens, Vibrio succinogenes, and Uniden-tified Strains. R. M. SMIBERT* AND LILLIAN V. HOLDEMAN .... ........... 432

Evaluation of the Bacti-Lab Streptococci Culture Systems for Selective Recoveryand Identification of Group A Streptococci. PAT W. FREEBURG* AND JUDY M.BUCKINGHAM.......................................................... 443

Mycobacterium Resembling Mycobacterium fortuitum That Produces Brown Pig-ment. JEAN E. HAWKINS* AND ELEANORA B. FALCO ..... ................ 453

Duodenal Isolation ofSalmonella typhi by String Capsule in Acute Typhoid Fever.ROBERT H. GILMAN* AND RICHARD B. HORNICK ...... ..................... 456

Use of CO2 for More Rapid Growth of the Nocardia Species. L. H. BONCYK,* C. H.MILLSTEIN, AND S. S. KALTER .......... ................................. 463

Virology, Rickettsiology, and ChlamydiologyViral Antibody in the Cerebrospinal Fluid of Patients with Acute Central Nervous

System Infections. RUDOLF DEIBEL AND GEORGE D. SCHRYVER .... ........ 397Elimination of Bacteriophages from Tissue Culture Serum by Affinity Chromatog-

raphy. HENRY C. ORR,* HOWARD H. WEETALL, PETER G. PROBST, DEWITT C.LITTLEJOHN, FRED C. CHU, JOYCE B. JOHNSON, AND JOHN C. PETRICCIANI ... 402

Improved Method for Production of Attenuated Venezuelan Equine Encepha-lomyelitis (TC-83 Strain) Vaccine. FRANCIS E. COLE, JR.,* CARL E. PEDER-SEN, JR., DAVID M. ROBINSON, AND GERALD A. EDDY ..... ................. 460

ImmunologyFluorescent Antibody Test for the Serological Diagnosis of Gonorrhea. HASSAN

A. GAAFAR* AND DORA C. D'A.RCANGELIS ................................ 438Simple Method for Immunofluorescent Identification of Mycoplasma Colonies.

JANET M. BRADBURY,* CHRISTINE A. ORIEL, AND F. T. W. JORDAN .... ..... 449Evaluation of the Beckman Antistreptolysin 0 and Antideoxyribonuclease B

(ASO/ADB) Test Kit. GEORGE C. KLEIN ................................ 458

EpidemiologyEvaluation of a Proteus rettgeri O-Serotyping System for Epidemiological Investi-

gation. JOHN L. PENNER,* NORMAN A. HINTON, AND JOAN N. HENNESSY .. 385

Nosocomial InfectionsApplication of a Gloved-Hand Model for Multiparameter Measurements of Skin-

Degerming Activity. R. N. MICHAUD,* M. B. MCGRATH, AND W. A. Goss . 406

Volume 3 Contents for May Number5

BacteriologyEffect of Sonic Treatment on Pure Cultures and Aggregates of Bacteria. S. A.

XVi CONTENTS

ROBRISH,* S. B. GROVE, R. S. BERNSTEIN, P. T. MARUCHA, S. S. SOCRANSKY,AND B. AMDUR......................................................... 474

Description of a Polyvalent Conjugate and a New Serogroup of Bacteriodesmelaninogenicus by Fluorescent Antibody Staining. DWIGHT W. LAMBE,JR.,* AND ROBERT C. JERRIS ......... ................................... 506

Application of a New Method for Detecting Streptococcal Nicotinamide AdenineDinucleotide Glycohydrolase to Various M Types of Streptococcus pyogenes.RuDoLF LUTTIcKEN,* DORIS LuTTrIcKEN, DWIGHT R. JOHNSON, AND LEWIS W.WANNAMAKER........................................................ 533

Virology, Rickettsiology, and ChlamydiologyPersistence of Antibody to Hepatitis B Surface Antigen. GILBERT R. IRWIN,*

ALFRED M. ALLEN, HERBERT E. SEGAL, MILTON WILLHIGHT, HUBERT CANNON,AND FRANKLIN H. Top, JR ............................................. 465

Hepatitis B Infection in Health Care Personnel of an Institution for MentallyHandicapped Children and Adults. MOTI L. TIKU, KARL R. BEUTNER,PATRICIA CARMODY, HARRY A. SULTZ, BANSI L. KAUL, AND PEARAY L. OGRA* 469

Antibody Response to Rocky Mountain Spotted Fever. R. H. KENYON,* P. G.CANONICO, L. S. SAMMONS, L. R. BAGLEY, AND C. E. PEDERSEN, JR......... 513

Purification of Influenza Virus with Arcton 113. MARGARET I. BRADY* AND I. G.S. FURMINGER ......................................................... 524

Comparison of Rhinovirus-Sensitive HeLa Cells and Human Embryo Fibroblastsfor Isolation of Rhinoviruses from Patients with Respiratory Disease. F. A.LEWIS* AND MARGERY L. KENNETT ...................................... 528

Procedure for Fluorescent-Antibody Staining of Virus-Infected Cell Cultures inPlastic Plates. ALFRED R. PURSELL* AND JOHN R. COLE, JR .............. 537

ImmunologyRadioimmunoassay for Measuring Antibodies Specific for Group B Streptococcal

Types Ia, Ib, Ic, II, and III. HAZEL W. WILKINSON* AND WALLIS L. JONES.. 480Enzyme-Linked Immunosorbent Assay for Streptococcal M Protein Antibodies.

H. RUSSELL,* R. R. FACKLAM, AND L. R. EDWARDS ........................ 501Direct Measurement of Microplates and Its Application to Enzyme-Linked Im-

munosorbent Assay. E. J. RUITENBERG,* B. J. M. BROSI, AND P. A.STEERENBERG......................................................... 541

EpidemiologyLaboratory Investigation of Diarrhea in Travelers to Mexico: Evaluation of

Methods for Detecting Enterotoxigenic Escherichia coli. GEORGE K. MOR-RIS,* MICHAEL H. MERSON, DAVID A. SACK, Joy G. WELLS, WILLIAM T.MARTIN, WALLIS E. DEWITT, JOHN C. FEELEY, R. BRADLEY SACK, AND DAVIDM. BESSUDO ........................................................... 486

Phage Typing of Staphylococcus epidermidis. HENRY W. TALBOT, JR., ANDJOSEPH R. PARISI*..................................................... 519

MycologyClinical Evaluation of the Addition of Gentamicin to Commercially Prepared

Mycological Media. W. G. MERZ, G. SANFORD, AND G. L. EVANS .......... 496

ERRATAPhage Typing Set for Group C, and C2 Salmonellae. M. GERSHMAN .... ...... 543Clinical Comparison of Aerobic, Hypertonic, and Anaerobic Culture Media for the

Radiometric Detection of Bacteremia. R. MARIE COLEMAN, W. WAYNELASLIE, AND D. W. LAMBE, JR.* ........ ................................ 543

x*iiCONTENTS

Volume 3 Contents for June Number 6Bacteriology

Serological Studies and Isolations of Serotype hardjo and Leptospira biflexaStrains from Horses of Argentina. DONALD M. MYERS ..... .............. 548

Significance of Urinary Isolates of Coagulase-Negative Micrococcaceae. D. N.WILLIAMS,* MARYLS E. LUND, AND DONNA J. BLAZEVIC ..... .............. 556

Rapid Method for Identification of Gram-Negative, Nonfermentative Bacilli. L.A. OTTO AND M. J. PICKETT* ............................................ 566

A Simplified Leifson Flagella Stain. W. A. CLARK .......................... 632Effect of Volume of Blood Cultured on Detection of Bacteremia. M. MARSHA

HALL, DUANE M. ILSTRUP, AND JOHN A. WASHINGTON II* ...... ........... 643

Virology, Rickettsiology, and ChlamydiologySystematic Variables Affecting Simian Virus 40-Induced T-Antigen Expression

and Transformation in Human Cells. M. M. KAPLAN, A. S. LUBINIECKI,*W. A. BLArrNER, T. MASON, D. J. GIARD, M. GuNNELL, N. TRIANTAFELLU, ANDJ. F. FRAUMENI, JR. .............................................. 593

Preparation of Rabies Fluorescein Isothiocyanate-Labeled Immune Globulin fromMouse Hyperimmune Ascitic Fluids. T. TZIANABOS,* G. A. HEBERT, ANDL. A. WHITE ............. ................................ 609

Large-Scale Purification of Hepatitis B Surface Antigen. JOHN VNEK AND AL-FRED M. PRINCE* .................... ......................... 626

Detection of Virus-Specific Immunoglobulins Using a Doubly Labeled Fluorescein-125I Antibody. A. J. PARKINSON* AND J. KALMAKOFF ....... .............. 637

ImmunologyCounterimmunoelectrophoresis of Staphylococcal Antibody. J. FEDORKO, B. E.

FOSSIECK, JR.,* AND R. H. PARKER ................ ...................... 599Rapid Micromethod of Screening for Antibodies to Disease Agents Using the

Indirect Enzyme-Labeled Antibody Concept. GEORGE C. SAUNDERS* ANDELVA H. CLINARD .................... ......................... 604

EpidemiologyCombined Serotyping and Biotyping of Serratia marcescens. SALLY J. RUBIN,*

SUSAN BROCK, MARY CHAMBERLAND, AND ROBERT W. LYONS ..... .......... 582Serogrouping of Bacteroides fragilis subsp. fragilis by the Agglutination Test.

DWIGHT W. LAMBE, JR.,* AND DAVID A. MOROZ ........ .................. 586

MycologyDelayed Hypersensitivity Cross-Reactions Between Sporothrix schenckii and

Ceratocystis Species in Sporotrichotic Patients. HIROSHI ISHIZAKI,* YOICHINAKAMURA, HIDEO KARIYA, TOKIO IWATSU, AND ROBERT WHEAT .... ....... 545

Long-Chain Fatty Acids of Sporothrix (Sporotrichum) schenckii. R. J. STRETTONAND R. K. DART*....................................................... 632

Comparative Isolation of Vaginal Yeasts on Selective and Nonselective Media.MARY D. BRASHEAR, JAMES WONG, PAULA HINES, AND RODNEY F. SMITH* .. 640

Nosocomial InfectionsDifferential Quantitation of Surface and Subsurface Bacteria of Normal Skin by

the Combined Use of the Cotton Swab and the Scrub Methods. CHARLESA. EVANS* AND ROBERT J. STEVENS ........ ............................. 576

xviii CONTENTS

CONTENTS xix

MycoplasmatologyMicrorespirometer Chamber for Determinations of Viability in Cell and Organ

Cultures. MICHAEL G. GABRIDGE ....................................... 560Differential Agar Medium (A7) for Identification of Ureaplasma urealyticum

(Human T Mycoplasmas) in Primary Cultures of Clinical Material. MAU-RICE C. SHEPARD* AND CARL D. LUNCEFORD .............................. 613

ERRATAEffects of Types of Media on the Production of Acid from Glucose by So-Called

Glucose-Negative Strains of Neisseria gonorrhoeae. ELLEN S. BARON* ANDARTHUR K. SAZ........................................................ 646

Rapid In Vitro Conversion and Identification ofCoccidioides immitis. S. H. SUN,M. HUPPERT,* AND K. R. VUKOVICH ..................................... 646