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CE-phosphite blocker 3’phosphoramidite
Contact: Shinzo Nakayama 5-1, Nihonbashi-Kobunacho, Chuo-ku, Tokyo 103-8355 JAPAN e-mail: [email protected] Phone: +81-3-3665-3383, Fax:+81-3-6535-3940
>99% de
OO
Bprot
O
OO
Bprot
O
PNCH2CH2CO
PNCH2CH2CO
DMTrOO
Bprot
O
PN(i-C3H7)2NCH2CH2CO
X
X
X
n = 1–4
O
OBprot
OO
OBprot
O
PXH2C=HCH2CO
PH2C=HCH2CO
X
DMTrOO
Bprot
O
PN(i-C3H7)2H2C=HCH2CO
n = 1–10
X
X
X
product diastereomers
allyl-phosphate blocker 3’-phosphoramidite
HPLC profile of the CE trimer phosphoramidite
stereo-controlled blocker 3’-phosphoramidite
O
OBprot
OO
OBprot
O
PSO
POS
DMTrOO
Bprot
O
PN(i-C3H7)2NCH2CH2CO
n = 1–4
X
X
X
N
N
R O
R O
*
*
HPLC profile of the stereo-controlled dimer
acetylated impurities
98% de (all Ps)
6 mer
13 mer
19 mer
monitoring of elongation process using blockmers in liquid phase
+7
+6
a 18 mer API synthesis using trimers on solid support
— Nucleotide Building Blocks —We deliver 1 - 100 g of building blocks for oligonucleotideAPI synthesis until 4 weeks in >98% purity. Blockmers are applied to 2’ modified nucleosides.
HPLC profile of the allyl hexamer phosphoramidite
fluorous tagged 3’-phosphoramidite
(mF-monomer)
a 20 mer synthesis using mF-monomers on solid support
HPLC profile of the mF-T monomer
>99% purity
18 mer
HPLC profile of the stereo-controlled tetramer
no shortmer impurities
Nucleic Acid Technologies
NATiAS
