NOVUS BIOLOGICALS ANTIBODIES FOR LIPID & METABOLISM RESEARCHimages.novusbio.com/design/lipid_catalog.pdf · LIPID & METABOLISM RESEARCH NOVUS IRELAND Phone: +353 1 506 0361 ... Journal

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LIPID & METABOLISM RESEARCH

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NOVUS BIOLOGICALS ANTIBODIES FOR

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Table of ContentsMetabolism & Epigenetics ..........2Pancreatic Islets ........................3ABC Transporters ......................4Obesity, Diabetes & Insulin Resistance ........................... 5-6Metabolism & Inflammation ... 7-8

Atherosclerosis ..........................9

CNS Control of Metabolism ....10

Neurodegeneration .........11-12

Tumor Metabolism ..........13-14

Application KeyELISA - Enzyeme-Linked Immunosorbent Assay

FACS - Fluorescent Activated Cell Sorting

ICC - Immunocytochemistry

IF - Immunofluorescence

IHC - Immunohistochemistry

IHC-Fr - Immunohistochemistry Frozen

IHC-P - Immunohistochemistry Paraffin

IP - Immunoprecipitation

PEP-ELISA - Peptide ELISA

WB - Western Blot

Reactivity KeyBv - Bovine

Ca - Canine

Ce - C. elegans

Ch - Chicken

ChHa - Chinese Hamster

Dr - Drosophila

Eq - Equine

Ha - Hamster

1

Hu - Human

Ma - Mammal

Mk - Monkey

Mu - Mouse

Po - Porcine

Rb - Rabbit

Rt - Rat

Sh - Sheep

Ze - Zebra Fish

Cover ImageScanning electron micrograph of adipocytes (orange) in human adipose connective tissue.

Making Your Success Our GoalNovus is committed to providing researchers with the highest quality antibodies so they may focus on their research. That’s why we’re continually working to provide the most comprehensive data available for our products. Its all available to you at www.novusbio.com.

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In addition to our primary antibody products, we offer an array of related research tools including immunoprecipitation and labeling kits, stem cell lines and a variety of secondary antibodies, lysates, peptides and proteins.

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• Acetoacetyl CoA synthetase• BMAL1• CLOCK• GAPDH• KAT3B/p300• NAD Synthetase

• NADK • NAMPT• NNMT• SIRT1• TPMT

Metabolism & EpigeneticsEpigenetic regulation via chromatin modifications is a growing field of research and has been shown to regulate many different gene pathways and pathologies. Many chromatin modifications require physiological metabolites resulting in epigenetic regulation that is directly affected by cellular metabolism. Chromatin modulators rely on essential cofactors that are linked closely to nutrient status. These cofactors include acetyl coenzyme A (A-coA), S-adenosyl methionine (SAM or AdoMet), and nicotinamide adenine dinucleotide (NAD)1. Epigenetic mechanisms have been implicated in the control of circadian rhythm and related metabolic path-ways. [PMID: 18662547] DNA methylation also plays a role in gene regulation of pancreatic islets from patients with type 2 diabetes. [PMID: 22293752]

2

Co-Factor Related Antibodies

Novus offers a full line of antibodies for DNA modification enzymes and modified histones, including our Epi-Plus® Antibodies, the most specific, well validated antibodies available for epigenetic research. Visit the Epigenetics Research area of our website for more information.

Epi-Plus®

BMAL1 Antibody NB100-2288

Species: Hu, Mu, Rt, MkApplications: WB, ICC, IF, IHC-P

Immuno- histochemicalanalysisin mousebrain using NB100-2288.

Customer Reviews

GAPDH (2D4A7) Antibody (NB300-328)Application: Western BlotSample Tested: HaCaT whole cell lysate Species: HumanResults: This antibody works great for Western blotting with little to no background, even at very dilute concentrations.

KAT3B/p300 Antibody (NB500-161)Application: WB, IP Sample Tested: Hypoxic hela cell Species: HumanChIP Type: Cross-linking (X-ChIP)

CLOCK Antibody NBP1-30326

Species: Hu, Mu, RtApplications: WB

Western blot analysis in Sol8 (mouse embryo fibroblast) cell extracts using NBP1-30326.

GAPDH (2D4A7) Antibody NB300-328

Species: Hu, MuApplications: WB, ELISA, ICC, IF, IHC-P

Immuno-cytochemicalanalysis (green) in HeLa cells using NB300-328.

KAT3B/p300 (RW105) Antibody NB100-616

Species: Hu, Mu, Rt, MkApplications: WB, IF, IP

Western blotanalysis in a HeLa nuclear extract using NB100-616.

KAT3B/p300 (RW109) Antibody NB100-617

Species: Hu, Mu, Rt, MkApplications: WB, IF, IP

Western blotanalysis in a HeLa nuclear extract using NB100-617.

SIRT1 AntibodyNBP1-49540

Species: HuApplications: WB, IHC

Immuno-histochemicalanalysis in human renal cell carcinomausing NBP1-49540.


• CD57• Chromogranin A• Glucose Transporter GLUT2 • Neuron-Specific Enolase (NSE)• Proprotein Convertase 2• Proprotein convertase PC4• Synaptophysin

Pancreatic Islets

3

Pancreatic islets are regions of the pancreas that contain the cells responsible for hormone production including glucagon, insulin, amylin, somatostatin, pancreatic polypeptide and ghrelin. These regions comprise 1-2% of the mass of the pancreas and are primarily involved in glucose homeostasis.

Metabolite Related Antibodies

• Amylin• Ghrelin• Glucagon• Glucagon Receptor• Insulin• Insulin Receptor• Insulin Receptor Beta• Insulin/Proinsulin

• IR Related Receptor • Pancreatic Polypeptide• Somatostatin• Somatostatin Receptor 1• Somatostatin Receptor 2• Somatostatin Receptor 3• Somatostatin Receptor 4• Somatostatin Receptor 5

Islet Markers

[CD57 Antibody NBP1-19788] Ichinose M. et al. Altered cytokine levels and increased CD4+CD57+ T cells in the peripheral blood of hepatitis C virus-related hepatocellular carcinoma patients. Oncol Rep. 2011 Jul;26(1):201-8. [PMID: 21491089][Chromogranin A Antibody NB120-15160] Polyak S, Mach A, Porvasnik S, et al. Identification of Adeno- Associated Viral Vectors Suitable for Intestinal Gene Delivery and Modulation of Experimental Colitis. American Journal of Physiology. Gastrointestinal and Liver Physiology. 2011 Nov 23. [PMID: 22114116][Glucagon Antibody NB100-91782] Rovira M, Scott SG, Liss AS, et al. Isolation and characterization of centroacinar/terminal ductal progenitor cells in adult mouse pancreas. Proc Natl Acad Sci USA. 2010 Jan 5;107(1):75-80. [PMID: 20018761][Somatostatin Receptor 2 Antibody NB300-157] Li F, Ryu BY, Krueger RL, et al. Targeted entry via somatostatin receptors using a novel modified retrovirus glycoprotein that delivers genes at levels comparable to wild type viral glycoproteins. Journal of Virology. 2011 Oct 19. [PMID: 22013043][Somatostatin Receptor 2 Antibody NB100-74537] Kao YJ, Ghosh M, Schonbrunn A. Ligand-Dependent Mechanisms of sst2A Receptor Trafficking: Role of Site-Specific Phosphorylation and Receptor Activation in the Actions of Biased Somatostatin Agonists. Mol Endocrinol. 2011 Apr 14. [PMID: 21493671][Synaptophysin Antibody NB300-653] Dash PK, Gorantla S, Gendelman HE, et al. Loss of Neuronal Integrity during Progressive HIV-1 Infection of Humanized Mice. J. Neurosci. 2011 Mar 2.[PMID: 21368026]

Product Citations

Chromogranin A (LK2H10) Antibody NB110-2475

Species: Hu, Rt, Mk, Po, RbApplications: WB, IHC-P

Immuno-histochemical analysis of rat pancreasusing NB110-2475.

CD57 Antibody NBP1-78982

Species: Hu, MuApplications: ICC, IF, IHC-P

Immuno-histochemical analysis in mousebrain usingDAB with hematoxylincounterstain usingNBP1-78982.

Glucose Transporter GLUT2 (5D1) AntibodyNBP1-40176

Species: HuApplications: FACS, ICC, IHC-P

Immuno-histochemical analysis inhuman liverusing NBP1-40176.

NSE (EPR3377) Antibody NBP1-40641

Species: Hu, Mu, RtApplications: WB, FACS, ICC, IHC-P

Immuno-histochemical analysis of embedded humanbrain tissueusing NBP1-40641.

Somatostatin Receptor 1 Antibody NB300-120

Species: Hu, Mu, RtApplications: WB, IP

Western blot analysis from rat pancreas using NB300-120.

Somatostatin Receptor 2 Antibody NB300-157

Species: Hu, Mu, RtApplications: WB, IF, IHC-Fr, IHC-P, IP

Immuno-histochemicalanalysis of rattissue usingNB300-157.

www.novusbio.com • [email protected] 4

Species: HuApplications: FACS, ICC, IHC-P

Immuno-histochemical analysis inhuman liverusing NBP1-40176.

Species: Hu, Mu, RtApplications: WB, IF, IHC-Fr, IHC-P, IP

Immuno-histochemicalanalysis of rattissue usingNB300-157.

NB400-105 ABCA1 Rabbit Polyclonal WB, FACS, ICC, IF, IHC-P, IP Hu, Mu, Rt, Ha 132

NB400-164 ABCA1 Rat Monoclonal WB Mu 2

NB400-165 ABCA1 Rat Monoclonal FACS Mu

NB100-2068 ABCA1 Mouse Monoclonal WB, IHC-P Hu, Mu,Rt 5

NB400-166 ABCA7 Rat Monoclonal WB, FACS Mu

NB400-163 ABCA7 Rat Monoclonal WB, FACS Mu

NBP1-77687 ABCB5 Rabbit Polyclonal ICC, IF, IHC-P Hu, Mu

NBP1-77689 ABCB5 Rabbit Polyclonal ICC, IF Hu

NB400-116 ABCE1 Rabbit Polyclonal WB, IP, ICC, IF Hu 1

NB400-115 ABCF2 Rabbit Polyclonal WB Hu, Mu, Ye

NB400-132 ABCG1 Rabbit Polyclonal WB, ICC, IF, IHC-P Hu, Mu 42

NB400-117 ABCG8 Mouse Monoclonal WB Hu, Mu 1

NB400-110 ABCG8 Rabbit Polyclonal WB Hu 2

NBP1-71706 ABCG8 Mouse Monoclonal WB, IF, IHC-P, IP Mu 4

NB110-41576 Abhd5 Rabbit Polyclonal WB Hu 3

ABC TransportersATP-binding cassette transporters (ABC transporters) are transmembrane proteins that transport a wide variety of substrates, including metabolic products, lipids and sterols, and drugs across both extra and intracellular membranes. ABC transporters are associated with a wide range of human diseases, including tumor resistance, cystic fibrosis, bacterial multi-drug resistance, and other inherited human diseases.

Catalog# Product Host Type Application Species References Customer Reviews

ABCA1 Antibody (3A1.891.3) (NB400-164)Application: Western BlotSample Tested: Brain microves-sels total proteins Species: Mouse

ABCA1 Antibody (NB400-105)Application: Western Blot Species: Mouse

ABCA1 Antibody NB400-105

Species: Hu, Mu, Rt, HaApplications: WB, FACS, ICC, IF, IHC-P, IP

Immuno-cytochemicalanalysis in HeLacells with FITC(green) usingNB400-105.

ABCB5 Antibody NBP1-77687


Immuno-histochemical analysis ofmouse spleenusing NBP1-77687.

ABCG1 Antibody NB400-132

Species: Hu, MuApplications: WB, ICC, IF, IHC-P

Immuno-histochemical analysis of lung, alveolar macrophages using NB400-132.

[ABCA1 Antibody NB400-105] McIntosh AL, Atshaves BP, Storey SM, et al. Loss of liver fatty acid binding protein significantly alters hepatocyte plasma membrane microdomains. Journal of Lipid Research. 2012 Jan 5. [PMID: 22223861][ABCA1 Antibody NB400-105] Storey SM, McIntosh AL, Huang H, et al. Intracellular Cholesterol Binding Proteins Enhance HDL-mediated Cholesterol Uptake in Cultured Primary Mouse Hepatocytes. American Journal of Physiology. Gastrointestinal and Liver Physiology. 2012 Jan 12. [PMID: 22241858][ABCA1 (HJ1) Antibody NB100-2068] de Souza Pinto R, Castilho G, Paim BA, et al. Inhibition of Macrophage Oxidative Stress Prevents the Reduction of ABCA-1 Transporter Induced by Advanced Glycated Albumin. Lipids. 2012 Jan 21. [PMID: 22271422][ABCG1 Antibody NB400-132] Ayaori M, Yakushiji E, Ogura M, Nakaya K, Hisada T, Uto-Kondo H, Takiguchi S, Terao Y, Sasaki M, Komatsu T, Iizuka M, Yogo M, Uehara Y, Kagechika H, Nakanishi T, Ikewaki K. Retinoic acid receptor agonists regulate expression of ATP-binding cassette transporter G1 in macrophages. Biochim Biophys Acta. 2012 Feb 14;1821(4):561-572. [PMID: 22353356][ABCG8 (1B10A5) Antibody NBP1-71706] Wang J, Grishin N, Kinch L, Cohen JC, Hobbs HH, Xie XS. Sequences in the nonconsensus nucleotide-binding domain of ABCG5/ABCG8 required for sterol transport. J Biol Chem. 2011 Mar 4;286(9):7308-14. [PMID: 14504269][Abhd5 Antibody NB110-41576] Timmers S, de Vogel-van den Bosch J, Hesselink M, et al. Paradoxical Increase in TAG and DAG Content Parallel the Insulin Sensitizing Effect of Unilateral DGAT1 Overexpression in Rat Skeletal Muscle. PLoS ONE 2011, 6(1). [PMID: 21264296]

Product Citations


Obesity, Diabetes & Insulin Resistance In the bloodstream, insulin acts to control blood sugar levels by regulating the uptake of glucose from the blood and glucose secretions from the liver. Under normal physiological conditions, adipose tissue can function as a signaling center, releasing adipokines which modulate this pathway. Under conditions of starvation or obesity, this cycle becomes perturbed and the action of the glucose transporter GLUT-4 is blocked, possibly through an increase in RBP4 release by adipocytes. This results in a failure of glucose uptake by the muscle and adipose tissue, while glucose secretion from the liver remains active. High levels of both glucose and insulin in the bloodstream cause a condition known as insulin resistance. Insulin resistance is considered a pre-diabetic state and can lead to the development of metabolic syndrome and Type 2 diabetes. Diseases associated with obesity include hypertension, dyslipidemia, type 2 diabetes, coronary heart disease, stroke, gallbladder disease, osteoarthritis, various cancers, sleep apnea and respiratory problems.

GLUT-4

RBP4

GlucoseRBP4

Liver

Glucose

GLUT-4Insulin

Muscle

Glucose

Related Antibodies

• Acetyl Coenzyme A Alpha• ACC1• Adiponectin• Adropin• AGPAT6• Alcohol Dehydrogenase• ATGL• Caveolin 1• Caveolin 2• Caveolin 3• CCKR• Ceramide Kinase• CFTR• DGAT1• Endothelial Lipase

• FADS1• Fatty Acid Synthase• GAD65• Gastric Lipase• GLUT1• GLUT4• HSL• Insulin Receptor• IRE1 alpha• Leptin• LPIN1• LXR• MRP 1• MRP 2• NOX4

• OXPAT• PDZK1• Perilpin• Perilpin A• Perilpin A+B• PGC-1 alpha• PGC-1 beta• PLTP• PPAR delta• RBP4• SCD• Sorbitol Dehydrogenase• SREBP1• TIP47• WFS1


[Caveolin 1 7C8) Antibody NB100-615] Munjal C, Tyagi N, Lominadze D, Tyagi SC. Matrix metalloproteinase 9 in homocysteine induced intestinal microvascular endothelial paracellular and transcellular permeability. Journal of Cellular Biochemistry. 2011 Nov 17. [PMID: 22275073][DGAT1 Antibody NB110-41487] Li M, Paran C, Wolins NE, Horowitz JF. High muscle lipid content in obesity is not due to enhanced activation of key triglyceride esterification enzymes or to the suppression of lipolytic proteins. Am J Physiol Endocrinol Metab. 2011 Feb 1. [PMID: 21285405][Endothelial Lipase Antibody NB400-118] Riederer M, Trbuši M, Degoricija V, Frank S. Endothelial Lipase Plasma Levels are Increased in Patients With Significant Carotid Artery Stenosis and History of Neurological Impairment. J Clin Med Res. 2012 Jan 15;4(1):49-51. [PMID: 22383927][IRE1 alpha [p Ser724] Antibody NB100-2323] Chen K, Jin P, He H-H, et al. Overexpression of Insig-1 protects beta cell against glucolipotoxicity via SREBP-1c. J Biomed Sci. 2011 Aug 16;18(1):57. [PMID: 21843373][IRE1 alpha [p Ser724] Antibody NB100-2323] Ota A, Wang Y. Cdc37/Hsp90-mediated regulation of IRE1[alpha] activity in ER stress response and insulin synthesis in INS-1 cells. The Journal of Biological Chemistry. 2011 Dec 23. [PMID: 22199355][NOX4 Antibody NB110-58851] Chen J, Chen JK, Harris RC. Angiotensin II Induces Epithelial-to-Mesenchymal Transition in Renal Epithelial Cells through Reactive Oxygen Species/Src/Caveolin-Mediated Activation of an Epidermal Growth Factor Receptor-Extracellular Signal-Regulated Kinase Signaling Pathway. Mol Cell Biol. 2012 Mar;32(5):981-91. [PMID: 22215616][Perilipin Antibody NB100-60554] Rigamonti A, Brennand K, Lau F, Cowan CA. Rapid cellular turnover in adipose tissue. PLoS ONE. 2011. [PMID 21407813][PGC-1 alpha Antibody NBP1-04676] Liu W, Singh R, Choi CS, et al. LDL Receptor Related Protein 6 (LRP6) regulates body fat and glucose homeostasis by modulating nutrient sensing pathways and mitochondrial energy expenditure. The Journal of Biological Chemistry. 2012 Jan 9. [PMID: 22232553][PGC-1 alpha Antibody NBP1-04676] Rebholz SL, Jones T, Burke KT, Jaeschke A, Tso P, D’Alessio DA, Woollett LA. Multiparity leads to obesity and inflammation in mothers and obesity in male offspring. Am J Physiol Endocrinol Metab. 2012 Feb;302(4):E449-57. [PMID: 22127227]

Product Citations

Customer Reviews

GLUT1 Antibody (NB110-39113)Application: Western BlotSample Tested: HeLa cell lysates Species: Human

NOX4 Antibody (NB110-58851)Application: Western BlotSample Tested: Rat liver whole homogenate/primary hepatocyte cell lysate Species: Rat

Fatty Acid Synthase Antibody (NB400-114)Application: Western BlotSample Tested: Rat Species: Rat Sample Pretreated: Doxorubicin

Adropin Antibody NBP1-26387

Species: Hu, Mu, Rt, BvApplications: WB

Western blot analysis on mouse brain lysate usingNBP1-26387.

AGPAT6 Antibody NB100-2390

Species: Hu, Mu, Rt, Bv, Mk, PoApplications: WB

Western blotanalysis inmouse skeletalmuscle usingNB100-2390.

Caveolin 1 (7C8) Antibody NB100-615

Species: Hu, Mu, RtApplications: WB, FACS, IF, IHC-P, IP

Immuno-fluorescentanalysis ofEaHy926 endothelial cell lineusing NB100-615.

GLUT1 Antibody NB110-39113

Species: Hu, Mu, Rt, Bv, Mk, RbApplications: WB, ICC, IF, IHC-P

Immuno-cytochemicalanalysis ofHeLa cells (green) using NB110-39113.

LPIN1 Antibody NB110-57150

Species: Hu, Mu, RtApplications: WB, IHC, IHC-P

Immuno-histochemical analysis ofLPIN1 in mousepancreas usingNB110-57150.

MRP1 (IU5C1) Antibody NB110-57131

Species: Hu, MuApplications: WB, IF

Immuno-fluorescentanalysis in HEK293cells usingNB110-57131.

Obesity, Diabetes & Insulin Resistance


Metabolism & Inflammation PPARs & LXRsThe nuclear receptors known as PPARs and LXRs are lipid-activated transcription factors. Recent studies indicate that they are key regulators of lipid metabolism and inflammation. PPARs and LXRs are activated by non- esterified fatty acids and cholesterol metabolites. Both control the expression of a range of metabolic and inflammatory genes. [PMID: 18650918]

• LXR• LXR alpha• LXR beta• PPAR alpha• PPAR delta• PPAR gamma

Customer Reviews

PPAR delta Antibody (NB600-637)Application: IHC-PSample Tested: Gerbil stomach Species: OtherSpecies Other: Gerbil

PPAR gamma Antibody (NB120-19481)Application: Western BlotSample Tested: Mouse BALB/c macrophage cell line Species: Mouse

NALP3 InflammasomeThe emergence of chronic inflammation during obesity has been associated with the interaction of several pathways. The Nod-like receptor (NLR) family of innate immune cell sensors including the NALP3 Inflommasome (also known as NLRP3 or cryopyrin) are implicated in recognizing signals leading to caspase-1 activation and subsequent interleukin-1β (IL1 beta) and IL-18 secretion. [PMID: 21217695]

• Caspase 1• IL18• IL1 beta• NALP3

LXR alpha Antibody NBP1-77106

Species: Hu, Mu, RtApplications: WB, ELISA, IHC-P

Immuno-histochemicalanalysis of ratliver tissue usingNBP1-77106.

LXR Antibody NB400-157

Species: Hu, Mu, Rt, Ch, MkApplications: WB

WB analysis of in human heart lysate usingNB400-157.

PPAR delta Antibody NBP1-39684

Species: Hu, Mu, Rt, Po, ShApplications: WB, ICC, IHC-P

Immuno-histochemicalanalysis ofhuman skeletalmuscle usingNBP1-39684.

IL18 Antibody NBP1-81607

Species: HuApplications: WB, IHC-P

Immuno-histochemicalanalysis of human lymphnode usingNBP1-81607.

NALP3 (Nalpy3-b) Antibody NBP1-97601

Species: HuApplications: WB, IHC-Fr, IP

Immuno-histochemicalanalysis in epithelial layer of human tonsil using NBP1-97601.

Related Antibodies

Related Antibodies

PPAR alpha Antibody NB600-636

Species: Mu, Rt, Bv, Ca, Ha Applications: WB, ELISA, IHC-P

Immuno-histochemicalanalysis of ratmouse livertissue usingNB600-636.

LXR beta Antibody NB100-1464

Species: HuApplications: WB, IHC-P, PEP-ELISA

Immuno-histochemicalanalysis ofhuman spleen usingNB100-1464.

PPAR gamma Antibody NBP1-61399


Immuno-histochemicalanalysis ofhuman placentatissue usingNBP1-61399.


Species: HuApplications: WB, IHC-Fr, IP


Immuno-histochemicalanalysis ofhuman spleen usingNB100-1464.


Unfolded Protein Response The endoplasmic reticulum (ER) is the major site in the cell for protein folding and trafficking and is central to many cellular functions. Failure of the ER’s adaptive capacity results in activation of the unfolded protein response (UPR), which intersects with many different inflammatory and stress signaling pathways. These pathways are also critical in chronic metabolic diseases such as obesity, insulin resistance, and type 2 diabetes. The ER and related signaling networks are emerging as a potential site for the intersection of inflammation and metabolic disease. [PMID: 20303879]

• ATF4• ATF6• CHOP/GADD153• Derlin1• eIF2A• GADD34

• GRP78• HSPA5• IRE1 alpha• SKIV2L2• XBP1

Customer Reviews

CHOP/GADD153 (9C8) Antibody (NB600-1335)Application: Western blotSample Tested: Rat heart tissue lysate Species: RatResults: We found it is also useful in rat tissue.

GADD34 Antibody (NB100-778)Application: Western BlotSample Tested: Whole cell lysateSample Loaded: 40 ug

[eIF2A (3D5) Antibody H00083939-M01] Rojas M, Arias CF, Lopez S. PKR is the kinase responsible for the phosphorylation of eIF2-alpha in rotavirus infection. J Virol. 2010 Jul 14. [PMID: 20631127][GRP78 Antibody 28220002] Gao D, Bambang IF, Putti TC, et al. ERp29 induces breast cancer cell growth arrest and survival through modulation of activation of p38 and upregulation of ER stress protein p58(IPK). Lab Invest. 2011 Nov 7. [PMID: 22064321][GRP78 Antibody NB100-91794] Audouard C, Le Masson F, Charry C, et al. Oocyte-targeted deletion reveals that hsp90b1 is needed for the completion of first mitosis in mouse zygotes. PLoS ONE. 2011;6(2):e17109. [PMID: 21358806][IL1 beta Antibody NB600-633] Paino A, Tuominen H, Jääskeläinen M, et al. Trimeric Form of Intracellular ATP Synthase Subunit [beta] of Aggregatibacter actinomycetemcomitans Binds Human Interleukin-1[beta]. PLoS One. 2011 Apr 18;6(4).[PMID: 21533109][NALP3 (8N8E9) Antibody NBP2-03947] De Nardo D, Latz E. NLRP3 inflammasomes link inflammation and metabolic disease. Trends Immunol. 2011 Aug;32(8):373-9. [PMID: 21733753][PPAR alpha (3B6/PPAR) Antibody NB300-537] Crespillo A, Suárez J, et al. Expression of the cannabinoid system in muscle: effects of a high-fat diet and CB1 receptor blockade. Biochem J. 2010 Dec 15;433(1):175-85

Product Citations

ATF6 Antibody NBP1-75478

Species: HuApplications: ICC, IF

Immuno-cytochemicalanalysis in HeLa cells with FITC (green) usingNBP1-75478.

CHOP/GADD153 (9C8) Antibody NB600-1335

Species: Hu, MuApplications: WB, IF, IHC-P, IP

Western blotanalysis of primary human fibroblasts. Lane 1: Untreated cells, Lane 2: Cells treated with tunicamycin

GRP78 Antibody NBP1-06274

Species: Hu, Mu, Rt, Bv, Ce, Ch, Dr, Mk, Sh, ZeApplications: WB, ICC, IF

Immuno-cytochemicalanalysis inHeLa cells with Dylight 488 (green)using NBP1-06274.

Metabolism & Inflammation

Related Antibodies

eIF2A (E90) [p Ser51] Antibody NB110-56949

Species: Hu, Mu, RtApplications: WB, ICC, IHC-P

Immuno-histochemicalanalysis ofhuman livercarcinoma usingNB110-56949.

XBP1 Antibody NBP1-77681


Immuno-cytochemicalanalysis inHeLa cells with with FITC (green)using NBP1-77681.

Derlin Antibody NB100-448

Species: Hu, Mu, Rt, Bv, Ca, Ch, Mk, ZeApplications: WB, IHC

Immuno-histochemicalanalysis of humanliver usingNB100-448.


AtherosclerosisHigh density lipoproteins (HDLs) play a critical role in cholesterol metabolism. Their plasma concentrations are inversely correlated with risk for atherosclerosis. The Scavenger Receptor Class B1 (SR-BI) binds HDLs with high affinity and mediates selective uptake of HDL cholesteryl ester. SR-BI is expressed primarily in liver and nonplacental steroidogenic tissues, and mediates selective cholesterol uptake by a distinct mechanism. Several Nox proteins, including Nox2 and Nox4, may contribute to increased intracellular oxidative stress in human coronary atherosclerosis in a cell-specific manner and thus may be involved in the genesis and progression of human coronary atherosclerotic disease. [PMID: 11914250]

NBP1-19168 CYBB/NOX2 Mouse Monoclonal WB Hu

NBP1-51928 CYBB/NOX2 Goat Polyclonal WB, PEP-ELISA Hu

NBP1-77008 HDL Chicken Polyclonal WB, ELISA, IF Hu, Mu, Rt

NBP1-76563 HDL Rabbit Polyclonal WB, ELISA Hu

NB110-58849 NOX4 Rabbit Polyclonal WB, ICC, IF, IHC-P Hu, Mu, Rt, Bv, Mk, Sh 7

NB110-58851 NOX4 Rabbit Polyclonal WB, ICC, IF, IHC-P Hu, Mu, Rt, Mk 11

NB400-101 SR-BI Rabbit Polyclonal WB, FACS, ICC, IF, IHC, IP Mu 64

NB400-131 SR-BI Goat Polyclonal WB, ICC, IF Hu, Mu, Rt 8

NB400-104 SR-BI Rabbit Polyclonal WB, FACS, ICC, IF, IHC-P, IP Hu, Mu, Rt 70

NB400-113 SR-BI Rabbit Polyclonal WB, B/N, ICC, IF, IP Hu, Mu 8

NB400-134 SR-BI/SR-BII Rabbit Polyclonal WB, ICC, IF, IHC, FACS Mu 3

NB400-102 SR-BII Rabbit Polyclonal WB, IF, IHC-P Hu, Mu, Rt, Bv 7

Catalog# Product Host Type Application Species References

CYBB/NOX2 Antibody NBP1-59062

Species: Hu, Mu, Rt, Ca, RbApplications: WB

Immuno-histochemical analysis of human lungusing NBP1-56062.

SR-BI Antibody NB400-104

Species: Hu, Mu, RtApplications: WB, FACS, ICC, IF, IHC-P, IP

Immuno-histochemical analysis ofadrenal cortexusing NB400-104.

SR-BI/SR-BII AntibodyNB400-134

Species: MuApplications: WB, ICC, IF, IHC

Immuno-cytochemicalanalysis inHeLa cells using NB400-134.

Customer Review

SR-BI Antibody (NB400-104)Application: FACSSample Tested: Mouse Species: MouseSample Pretreated: ParaformaldehydeResults: The signal is strong and clear

[SR-BI Antibody NB400-101] Saha S, Graessler J, Schwarz PE, et al. Modified high-density lipoprotein modulates aldosterone release through scavenger receptors via extra cellular signal-regulated kinase and Janus kinase-dependent pathways. Mol Cell Biochem. 2012 Mar 1. [PMID: 22382638][SR-BII Antibody NB400-102] Kushiyama A, et al. Xanthine oxidoreductase is involved in macrophage foam cell formation and atherosclerosis development. Arterioscler Thromb Vasc Biol. 2012 Feb;32(2):291-8. [PMID: 22095983][SR-BI Antibody NB400-104] Molina-Jimenez F, Benedicto I, Dao Thi VL, et al. Matrigel-embedded 3D culture of Huh-7 cells as a hepatocyte-like polarized system to study hepatitis C virus cycle. Virology. 2012 Jan 24. [PMID: 22280897][SR-BI Antibody NB400-104] Storey SM, McIntosh AL, Huang H, et al. Intracellular Cholesterol Binding Proteins Enhance HDL-mediated Cholesterol Uptake in Cultured Primary Mouse Hepatocytes. American Journal of Physiology. Gastrointestinal and Liver Physiology. 2012 Jan 12. [PMID: 22241858][SR-BI Antibody NB400-113] Kim K-Y, Stevens MV, Akter MH, et al. Parkin is a lipid-responsive regulator of fat uptake in mice and mutant human cells. J Clin Invest. 2011 Aug 25. [PMID: 21865652][SR-BI/SR-BII Antibody NB400-134] Hu J, Zhang Z, Shen W-J, et al. Differential Roles of Cysteine Residues in Cellular Trafficking, Dimerization, and Function of the HDL Receptor, SR-BI. Biochemistry. 2011 Nov 18. [PMID: 22097902]

Product Citations

SR-BI Antibody NB110-58849

Species: Hu, Mu, Rt, Bv, Mk, ShApplications: WB, ICC, IF, IHC-P

Immuno-histochemical analysis in proximal convoluted tubules of the kidney using NB110-58849.


Species: Hu, Mu, Rt, Ca, RbApplications: WB

Immuno-histochemical analysis of human lungusing NBP1-56062.

Species: MuApplications: WB, ICC, IF, IHC

CNS Control of Metabolism Leptin & Insulin Adiposity SignalsFood intake is controlled by a highly complex process. Compounds that affect food intake and that are critical for normal energy homeostasis are termed adiposity signaling molecules. Leptin and insulin are two molecules that act through intermediaries as adiposity signals. Several distinct hypothalamic neuropeptide-containing pathways have emerged as candidate mediators of leptin and insulin action in the central nervous system (CNS). [PMID: 10766253]

Related Antibodies

• AGRP• CARTPT• CRHBP• Galanin• GalR1• GalR2• GalR3• GALP• GLP1R• IL-1 Receptor beta• Insulin • Insulin Receptor• Insulin Receptor beta• IR-realted receptor

• Leptin• Leptin Receptor • MCHR• MCHR1• MCHR2• MRAP2• Neuropeptide Y• Neurotensin• Neurotensin Receptor 1• Neurotensin Receptor 2• Noradrenaline transporter• NPY1R• NPY2R• NPY4R

• NPY5R• Orexin• Orexin A• Orexin Receptor 1• Orexin Receptor 1/2• Orexin Receptor 2• Oxytocin• Oxytocin Receptor• Serotonin• Thyrotropin Releasing Hormone• Thyrotropin Releasing Hormone Receptor• Urocortin

Customer Review

Orexin Receptor 2 Protein (H00003062-Q01)Application: Western BlotSpecies: Human

CRHBP Antibody NBP1-91811


Immuno-histochemical analysis of human placenta using NBP1-91811.

GLP1R Antibody NBP1-97308

Species: Hu, MuApplications: WB, ICC, IF, IHC-P

Immuno-histochemical analysis in mouse pancreas using NBP1-97308.

[GALR1 Antibody NLS4105] Barson JR, Chang G-Q, Poon K, et al. Galanin and the orexin 2 receptor as possible regulators of enkephalin in the paraventricular nucleus of the hypothalamus: relation to dietary fat. Neuroscience. 2011 Jul 28. [PMID: 21821102][Neuropeptide Y (2C10) Antibody H00004852-M03] Yang Y, Fung SJ, Rothwell A, et al. Increased interstitial white matter neuron density in the dorsolateral prefrontal cortex of people with schizophrenia.Biol Psychiatry. 2011 Jan 1;69(1):63-70.[PMID: 20974464]

Product Citations

Leptin Receptor Antibody NBP1-19740


Immuno-histochemical analysis ofhuman braintissue using NBP1-19740.

Oxytocin Receptor Antibody NBP1-02410

Species: HuApplications: IHC-P

Immuno-histochemical analysis of human pregnantuterus usingNBP1-02410.

Serotonin (5HT-H209) Antibody NB120-16007

Species: Hu, Mu, Rt, GPApplications: ICC, IF, IHC-Fr, IHC-P

Immuno-histochemical analysis of human pregnantuterus usingNBP1-02410.


NeurodegenerationLipid storage diseases, or lipidoses, are a group of inherited metabolic disorders in which harmful amounts of lipids accumulate in certain cells and tissues. People with these disorders do not produce enough of one of the enzymes needed to metabolize lipids or produce enzymes that do not work properly. Over time, this accumulation of fats can cause permanent cellular and tissue damage, particularly in the brain, peripheral nervous system, liver, spleen, and bone marrow. Niemann-Pick disease is an autosomal recessive disorder resulting in an accumulation of fat and cholesterol in cells of the liver, spleen, bone marrow, lungs, and in some patients, the brain.

The LDL receptor (LDLR) family is comprised of several multifunctional cell surface proteins. One ligand common to all LDLR family members is apolipoprotein E (ApoE), a lipid transport protein that also plays a central role in the pathogenesis of neurodegeneration in Alzheimer’s disease. [PMID: 14657206] There is also evidence linking Reelin with neurodegeneration through binding of ApoE receptors and as a mediator of Tau hyperphosphorylation. [PMID: 15583703]

Apolioprotein Antibodies

• ApoE• ApoE4• ApoER2• Apolipoprotein AI• Apolipoprotein AII• Apolipoprotein A5• Apolipoprotein B• Apolipoprotein A1• Apolipoprotein C1

• Apolipoprotein CII• Apolipoprotein CIII• Apolipoprotein D• Apolipoprotein F• Apolipoprotein H• Apolipoprotein J• Apolipoprotein L• Apolipoprotein M

Customer Review

Apolipoprotein CIII Antibody (NB600-610)Application: ELISASample Tested: Human serumSpecies: HumanLot: 21761

ApoE (WUE-4) Antibody NB110-60531

Species: HuApplications: WB, ELISA, IHC

Western blotanalysis in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain

ApoE4 (4E4) Antibody NBP1-49529

Species: HuApplications: WB, ELISA, IP

Western blotanalysis in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using NBP1-49529.

ApoER2 AntibodyNB100-2216

Species: Hu, Mu, ChApplications: WB

Western blot analysis of ApoER2 on mouse brain membrane usingNB100-2216.

Apolipoprotein A5 (1G5G9) Antibody NB110-55454

Species: HuApplications: WB, ELISA, IHC-P

Western blotanalysis in HepG2 lysates using NB110-55454.

Apolipoprotein A5 (1G5G9) Antibody NB400-139

Species: HuApplications: WB, ELISA, IHC-P

Western blotanalysis in in HepG2 cell lysate with NB400-139.

Apolipoprotein D (EPR2916) AntibodyNBP1-95270

Species: HuApplications: WB, ICC, IHC-P

Immuno-histochemical analysis ofhuman liver tissue using NBP1-95270.

Apolipoprotein F Antibody NBP1-20882


Immuno-histochemical analysis of humanliver usingNBP1-20882.

Apolipoprotein J Beta Chain Antibody NBP1-90128


Immuno-histochemical analysis ofhuman pancreasusing NBP1-90128.

Apolipoprotein L2 AntibodyNBP1-55937

Species: HuApplications: WB, ELISA, IF, IHC-P

Immuno-histochemical analysis ofhuman cervixcarcinoma tissueusing NBP1-55937.


Species: Hu, Mu, ChApplications: WB

Western blot analysis of ApoER2 on mouse brain membrane usingNB100-2216.

Apolipoprotein D (EPR2916) AntibodyNBP1-95270

Species: HuApplications: WB, ICC, IHC-P

Immuno-histochemical analysis ofhuman liver tissue using NBP1-95270.

Apolipoprotein L2 AntibodyNBP1-55937


Immuno-histochemical analysis ofhuman cervixcarcinoma tissueusing NBP1-55937.

Related Antibodies

• AKT1• AMACR• Amyloid Precursor Protein• Cathepsin E• Ceramide Kinase• CYP46A1• DAB1• EGF• Fatty Acid Synthase• GSK3 beta• IRP2• JIP2• JIP3• LDL Receptor• LRP1B

• LRP5/6• LRP6• Macrophage Scavenger Receptor I• MC3 Receptor• Niemann-Pick C1• Niemann-Pick C1L1• NMDA• PI 3 Kinase• PIK3CA• Reelin• RNF139• Tau• Thimet Oligopeptidase• VLDL Receptor• WFS1

LDL Receptor (EP1553Y) Antibody (NB110-57162)Application: Western blotSample Tested: Cell lines (Hep2 HepG2) Results: So far the best antibody for LDL Receptor.

NMDAR1 Antibody (NB100-92192)Application: IHC-P Sample Tested: RatSpecies: Rat

[ApoE Antibody NB100-2040] Terwel D, Steffensen KR, Verghese PB, et al. Critical Role of Astroglial Apolipoprotein E and Liver X Receptor-alpha Expression for Microglial A beta Phagocytosis. J Neurosci. 2011 May 11;31(19):7049-59. [PMID: 21562267][ApoE (WUE-4) Antibody NB110-60531] Lee CY, Tse W, Smith JD, Landreth GE. Apolipoprotein E Promotes ß-Amyloid Trafficking and Degradation by Modulating Microglial Cholesterol Levels. J Biol Chem. 2012 Jan 13;287(3):2032-44. [PMID: 22130662][LDL Receptor Antibody NBP1-06709] Guo H, Zhang Y, Brockman DA, et al. Lipocalin 2 Deficiency Alters Estradiol Production and Estrogen Receptor Signaling in Female Mice. Endocrinology. 2012 Jan 10. [PMID: 22234464][LDL Receptor (EP1553Y) Antibody NB110-57162] Domenger D, Dea D, Theroux L, et al. The MPTP neurotoxic lesion model of Parkinson’s disease activates the apolipoprotein E cascade in the mouse brain. Experimental Neurology. 2011. [PMID: 22155743]

Product Citations

Customer Reviews

NMDAR1 Antibody (NB400-114)Application: Western blotSample Tested: RatSpecies: Rat

Cathepsin E Antibody NB400-152

Species: Hu, Mu, RtApplications: WB, IF, IHC-P

Immuno-histochemicalanalysis in human renal cancer usingNB400-152.

Ceramide Kinase Antibody NB100-2911

Species: HuApplications: WB

Western blotanalysis in A549 cell lysate using NB100-2911.

CYP46A1 AntibodyNB400-140


Blocking experiment using NB400-140.

LDL Receptor Antibody NBP1-06709

Species: Hu, Mu, MkApplications: WB

Western blot analysis on HepG2 whole cell lysate usingNBP1-06709.

Macrophage Scavenger Receptor I Antibody NBP1-00093

Species: Hu, Mu, Rt, Bv, Ca, Eq, MkApplications: WB, ICC, IF

Immuno-cytochemicalanalysis in HeLacells usingNBP1-00093.

Niemann-Pick C1 AntibodyNB400-148

Species: Hu, Mu, ChHaApplications: WB, IF, IHC-P, IP

Immuno-histochemicalanalysis in brain, cortex, neurons andastrocytes usingNB400-148.

Neurodegeneration

12


Tumor MetabolismEarly in the last century, theories abounded as to how cancer arose in the body. Nobel laureate Otto Warburg hypothesized that malignant growth occurred as a consequence of cells utilizing non-oxidative metabolism of glucose as opposed to oxidative respiration. Current understanding of genetic alterations in many of the known oncogenes shows that the alterations aid the tumor in adapting cellular metabolism to meet the requirements of rapid cell proliferation as well as autonomous growth and survival in an environment absent of contact with extracellular matrix. Accumulating evidence indicates that almost every known oncogene regulates downstream targets that are directly connected to metabolic regulation. [PMID: 20598111]

Related Antibodies

• ADFP• AMACR• ARH• AS3MT• ATF6• Cathepsin E• Caveolin 1• CBR3• Ceramide Kinase• CHREBP• CLPP• CPT1B1• CYP7B1• Cytochrome P450• DCXR• EDG1• EDG2

• elF2• GADD34• GLUT1• Importin 13• Lipocalin 2• Macrophage Scavenger Recptor 1

• MafA

• MafB

• MARCKS

• MCT2

• MRP1

• MRP4

• MTCH1

• ORP1

• OSBP

• OSBP1

• OSBP2• OSBPL2• OSBPL6• OSBPL7• OSBPL9• OSBPL10• OSBPL11• PCK1• PDHX• PDK1• PI-15• PTEN• SCARF1• SRD5A1• STARD3• TXNRD1• VDAC2

ADFP Antibody NB110-40877

Species: Hu, MuApplications: WB, ICC, IF

Immuno-cytochemicalanalysis in HeLa cells usingNB110-40877.

AMACR Antibody NBP1-50557


Immuno-histochemicalanalysis onhuman kidney using NBP1-50557.

ATF6 AntibodyNBP1-75478

Species: HuApplications: ICC, IF

Immuno-cytochemicalanalysis in HeLa cells withFITC (green) using NBP1-75478.

CBR3 Antibody NBP1-87065

Species: HuApplications: WB, IF, IHC-P

Immuno-fluorecentanalysis showingpositivity in nucleus but not nucleoli & cytoplasm usingNBP1-87065.

CHREBP Antibody NB400-136

Species: Hu, MuApplications: IF

Immuno-fluorescentanalysis of ahepatocyte using NB400-136.

CHREBP AntibodyNB400-135

Species: Hu, Mu, RtApplications: WB, ChIP, IF, IHC-P

Immuno-histochemicalanalysis inhuman colon,epitheliumusing NB400-135.

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Customer Reviews

GADD34 Antibody (NB100-778)Application: Western blotSample Tested: Whole cell lysate

Lipocalin 2 Antibody (NB100-1503)Application: Western BlotSample Tested: Human Xenograft Tumor Species: HumanSample Pretreated: Paraffin embedded

PDK1 Antibody (NB100-2383)Application: Western BlotSample Tested: HeLa cell lysates

CHREBP Antibody (NB400-135)Application: Western blotSample Tested: Aorta whole tissueSpecies: Mouse


[ADFP Antibody NB110-40877] Wohlers LM, Jackson KC, Spangenburg EE. Lipolytic signaling in response to acute exercise is altered in female mice following ovariectomy. J Cell Biochem. 2011 Aug 3. [PMID: 21815195]

[ARH Antibody NB100-1276] Ye Z-J, Go G-W, Singh R, et al. LRP6 regulates LDLR-mediated LDL uptake. The Journal of Biological Chemistry. 2011 Nov 28. [PMID: 22128165]

[Cytochrome P450 17A1 Antibody NB100-2842] Efstathiou E, Titus M, Tsavachidou D, Tzelepi V, Wen S, Hoang A, Molina A, Chieffo N, Smith LA, Karlou M, Troncoso P, Logothetis CJ. Effects of abiraterone acetate on androgen signaling in castrate-resistant prostate cancer in bone. J Clin Oncol. 2012 Feb 20;30(6):637-43. [PMID: 22184395]

[Cytochrome P450 17A1 Antibody NB100-92420] Jeong CW, Yoon CY, Jeong SJ, et al. Limited expression of cytochrome p450 17[alpha]-hydroxylase/17,20-lyase in prostate cancer cell lines. Korean J Urol. 2011 Jul;52(7):494-497. [PMID: 21860772]

[MRP4 Antibody NB100-1471] Naud J, Michaud J, Beauchemin S, et al. Effects of chronic renal failure on kidney drug transporters and cytochrome P450 in rats.Drug Metab Dispos. 2011 Aug;39(8):1363-9. [PMID 21525170]

[OSBP1 Antibody NB100-1500, OSBPL10 Antibody NB100-2448, OSBPL11 Antibody NB100-1337, OSBPL2 Antibody NB100-1332 and OSBPL9 Antibody NB100-1336] Storey SM, McIntosh AL, Huang H, et al. Intracellular Cholesterol Binding Proteins Enhance HDL-mediated Cholesterol Uptake in Cultured Primary Mouse Hepatocytes. American Journal of Physiology. Gastrointestinal and Liver Physiology. 2012 Jan 12. [PMID: 22241858]

[PDK1 Antibody NB100-2383] Luo W, Hu H, Chang R, et al. Pyruvate Kinase M2 is a PHD3-Stimulated Coactivator for Hypoxia-Inducible Factor 1.Cell. 2011 May 27;145(5):732-44. [PMID: 21620138]

[PDK1 Antibody NB100-2384] Lin HP, Jiang SS, Chuu CP. Caffeic Acid Phenethyl Ester Causes p21 Induction, Akt Signaling Reduction, and Growth Inhibition in PC-3 Human Prostate Cancer Cells. PLoS One. 2012;7(2):e31286. [PMID: 22347457]

Product Citations

EDG1 Antibody NBP1-76942


Immuno-histochemicalanalysis ofmouse thymustissue usingNBP1-76942.

EDG2 Antibody NLS212

Species: HuApplications: IHC-P

Immuno-histochemicalanalysis inadrenalusing NLS212.

Lipocalin 2 AntibodyNB100-1503

Species: HuApplications: WB, IHC, PEP-ELISA

Immuno-histochemicalanalysis ofhuman spleen using NB100-1503.

Tumor Metabolism

OSBPL6 Antibody NBP1-31456


Immuno-histochemicalanalysis ofSCC15 usingNBP1-31456.

OSBPL10 Antibody NBP1-86153


Immuno-histochemicalanalysis of humannasopharynxusing NBP1-86153.

PTEN AntibodyNBP1-73972

Species: Hu, Mu, RtApplications: WB, ELISA, IHC-P, IP

Immuno-histochemicalanalysis ofhuman prostatetissue usingNBP1-73972.

SCARF1 (EPR3848) Antibody NBP1-40779

Species: HuApplications: WB, ICC, IHC-P, IP

Immuno-histochemicalanalysis ofhuman lungtissue usingNBP1-40779.

Txnrd1 Antibody NBP1-81791


Immuno-fluorescentanalysis showingpositivity in cytoplasm usingNBP1-81791.

VDAC2 Antibody NBP1-89477


Immuno-histochemicalanalysis ofhuman cerebellumusing NBP1-89477.


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NOVUS BIOLOGICALS ANTIBODIES FOR LIPID & METABOLISM RESEARCHimages.novusbio.com/design/lipid_catalog.pdf · LIPID & METABOLISM RESEARCH NOVUS IRELAND Phone: +353 1 506 0361 ... Journal