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Novel Technologies in Mass Rearing of
Beneficial Arthropods
Shimon Steinberg, BioBee Sde Eliyahu Ltd.ISRAEL ABIM, Basel, October 2013
Macrobial natural enemies for biological pest control
BioBee Sde Eliyahu Ltd. Produces, markets and implementscomprehensive solutions by means of:
Bumblebees for natural pollination
Sterile Mediterranean fruit flies for medfly control using Sterile Insect Technique (SIT)
Biopesticides - To complement the natural enemy’s package
BioBee
• Established 1983
• Two hundred employees in Israel and
in 3 subsidiaries
• The majority of the produce is
exported to 100 destinations world-
wide
BioBee
Production of P. persimilis
Off-Plant Production of the Predatory Mite:
Phytoseiulus persimilis
Production of P. persimilis
Modified Tri-trophic System
P. persimilisSpider-miteBean
Production of P. persimilis
• Indoor (=climate room) controlled rearing system
Production of P. persimilis
• Indoor (=climate room) controlled rearing system
• The product: age distribution includes 30-50% eggs
Production of P. persimilis
• Indoor (=climate room) controlled rearing system
• The product: age distribution includes 30-50% eggs
• The product: well fed gravid females capable of
surviving storage and transport and establish
rapidly in the field
P. persimilis rate of establishment
Tomato | 2012
Spider mites population dynamics
Tomato | 2012
Prey/predator ratio
Tomato | 2012
BugFeed®
Inactivated Frozen Medfly Eggs
BugFeed®
• Alternative to Ephestia eggs as feed for mass
rearing of predatory bugs, predatory beetles,
others?
BugFeed®
• Alternative to Ephestia eggs as feed for mass
rearing of predatory bugs, predatory beetles,
others?
• One year storage capacity
BugFeed®
• Alternative to Ephestia eggs as feed for mass
rearing of predatory bugs, predatory beetles,
others?
• One year storage capacity
• Patent granted. Product on sale
BugFeed®
BugFeed® | as Good as Ephestia Eggs
Orius laevigatus mass rearing
0
10000
20000
30000
40000
50000
Ephestia eggs 8 months stored
BugFeed®
Mixed diet
No. of
indiv
idual
s co
llec
ted
Diet
Low feeding
High feeding
0
2
4
6
8
10
Ephestia eggs BugFeed®
% M
ort
alit
y
Diet
High feeding
Low feeding
Mealybug Parasitoid’s Mummies
Mealybugs’ Mummies
Anagyrus pseudococciLeptomastix algirica
Mealybugs’ Mummies
Mealybugs’ Mummies
• A robust rearing system that can be applied to a number of mealybug parasitoids
Mealybugs’ Mummies
• A robust rearing system that can be applied to a number of mealybug parasitoids
• A breakthrough in delivery of mealybugparasitoids’ commercial products to the field
Mealybugs’ Mummies
• A robust rearing system that can be applied to a number of mealybug parasitoids
• A breakthrough in delivery of mealybugparasitoids’ commercial products to the field
• Patent granted (national phase). Two products on sale
Mealybugs’ Mummies
R² = 0.9167
R² = 0.8949
1
2
3
4
5
6
7
8
9
0
10
20
30
40
50
60
70
80
90
100
0 5 10 15 20 25 30
Avera
ge
eg
gs
/fem
ale
Em
erg
en
ce r
ate
(%
)
storage (days)
Anagyrus pseudococci(a) Emergence rate
(b) Fertility
R² = 0.9872
R² = 0.9832
1
2
3
4
5
6
7
8
9
10
0
10
20
30
40
50
60
70
80
90
100
0 5 10 15 20 25 30 35 40 45A
vera
ge
eg
gs
/fem
ale
Em
erg
en
ce r
ate
(%
)
Storage (days)
Leptomastix algirica
(a) Emergence rate
(b) Fertility
Post-storage capacity | @12°C
Mealybugs’ Mummies
0.05.0
10.015.020.025.030.035.040.0
15
.11
.20
11
29
.11
.20
11
12
.12
.20
11
29
.12
.20
12
14
.01
.20
12
28
.1.2
01
2
13
.2.2
01
2
5.3
.20
12
19
.3.2
01
2
7.0
4.2
01
2
21
.04
.20
12
% f
ruit
infe
ste
d
Robyn - % fruit infestation by mealybug
Control
2500/ha
5000/ ha
Moore et al. 2012
Anagyrus field trials in citrus | Western Cape, S.A
Mealybugs’ Mummies
Moore et al. 2012
Anagyrus field trials in citrus | Western Cape, S.A
Augmentation with Anagyrus sp. nrpseudococci should be conducted at a rate ofat least 5000 parasitoids per ha in the firstyear, and possibly a lesser quantity from thesecond year onwards.
Conclusion
Take Home Message
Mass rearing of macrobial biocontrol agents is an everlasting challenge
BioBee has developed novel rearing techniques which contribute significantly to its commercial products’ Availability-Consistency-Quality
Thank you!
www.biobee.com