Norton group meeting 11/2010, David Neff

Comparison of excitation output Hg vs HgXe vs Xe lamp at objective of

TE200 with Optisplit:

justification for 2” optics necessary to efficiently couple Xe lamp to

o-split scope

HgXe lamp Hg lampNo lamp (ambient, lights off in room but fluor lights in main area leaking in through window)

Input optics on HgXe lamp are inferior so do not directly compare absolute intensities. Relative intensities at different wavelength are valid.

position 4 GFPex 470/40

bs 495LP

em 525/50

position 2 rhodex 560/40

bs 595LP |em 630/60

position 1 osplitex 470/40&550/20

bs 520/40&590/60

em 540LP-green 520/40 -red 595/50

this is the output straight from the lamp

this is the output straight from the lampno lamp

Xe lampNo lamp (ambient, room lights off but light leak in window from main room)

Input optics on HgXe and Xe lamp are inferior so do not directly compare absolute intensities. Relative intensities at different wavelength are valid.

position 4 GFPex 470/40

bs 495LP

em 525/50

position 2 rhodex 560/40

bs 595LP |em 630/60

position 1 osplitex 470/40&550/20

bs 520/40&590/60

em 540LP-green 520/40 -red 595/50

no lamp

this is the output straight from the lamp

In the scope:•59004x dual-band exciter•59004bs polychroic mirrorIn the Optosplit:•ET515/30m for GFP•ET595/50m for rhodamine•T540LPXR

biorad mrc1024 leica sp5

20x

100x

notice all the contrast in the Leica images almost make them look 'worse'.