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Nobel Prize in Physiology or Medicine - 1974 For “the structure and functional organization of the cell”. George E. Palade. Albert Claude. Christian de Duve. Modern Cell Biology – Structure and Function A Membrane Bound Perspective. 1938 - Electron microscope available - PowerPoint PPT Presentation
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Nobel Prize in Physiology or Medicine - 1974For “the structure and functional organization of the cell”
Christian de Duve George E. PaladeAlbert Claude
Modern Cell Biology – Structure and FunctionA Membrane Bound Perspective
1938 - Electron microscope available
Albert Claude – Rockefeller Institute used EM for visualizing cells Invented differential centrifugation and went on to discover the endoplasmic reticulum
George Palade – Discovered ribosomes and determined that they carry out protein synthesis
Went on to show that vesicles travel from ER to golgi to outside of the cell
Christian de Duve – Used fractionation to isolate groups of enzymes discovered lysosome and perioxisome
A WELL-KNOWN TECHNIQUE FOR USING 35S OR 3H TO STUDY PROTEIN MOVEMENT
WITHIN A CELL
After 10 minutes, insulinmoves from ER to Golgi
Pulse of pancreatic B cells with 3H
After 45 minutes, the protein is found in the secretory granules
Flourescence Recovery after photobleaching (FRAP)Flourescence loss in photobleaching (FLIP) pg 608 in your book
Three main types of coated vesicles:
Clathrin is also involved in receptor mediated endocytosis
http://www.abcam.com/
BOTH Clathrin and COPI/II assembly requires GTPases