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Nitrogen efficiency of dairy sheep supplemented with crude glycerin diluted in water* Natália C. Alves 1** , José A. Junior 2 , Felipe S. Santos 2 , Hemilly C. M. Sá 3 , Iran Borges 2 , Gilberto L. Macedo 4 , Tassia L. T. M. 2 , Jizênio A. S. Passos 1 , Juliana A. Favato 1 , Celso H. F. Paiva 1 *Part of master thesis of the third author 1 Undergraduate student Veterinary School of Federal University of Minas Gerais; 2 Animal Science Department, Veterinary School of Federal University of Minas Gerais; 3 Animal Science Department, Federal University of Sergipe; 4 Animal Science Department, Federal University of Uberlândia; **Federal University of Minas Gerais, Av. Presidente Carlos Luz, 4466, São Francisco, CEP 31250-810, Belo Horizonte, MG, Brazil; [email protected] The Crude Glycerin (CG) expressed a potential for use in diet of dairy ruminants. Recent renewable energy use policies have promoted a CG surplus. This byproduct can be applied to ruminant nutrition as a cheap source of energetic food, with easily storage and delivery. The use of CG can provide balance between energy and protein, according to ruminal degradation kinetics, that results in maximum microbial growth, in lower urinary losses of energy and nitrogen, and optimization of dietary nitrogen use for casein synthesis. The objective of this study was to evaluate the effects of the inclusion of crude glycerin diluted in water for dairy sheep on nitrogen (N) efficiency for milk yield. Were used 20 Lacaune x East Friesian lactating dairy ewes, between three and four years’ age, with the average weight of 62.68 ± 5.95kg. The animals remained in individual pen with free access to water, and concentrate and roughage were provided separately. The sheep were distributed in a completely randomized design, in four treatments differed as to supplementary CG intake diluted in water: zero, 1.5%, 3.0% and 4.5% of dry matter intake. Ewes were Machine milked once a day at 15:00. The lactation persisted to 17 weeks. The diets were formulated to meet the nutritional requirements according to the NRC (2007). Weekly an individual sample of 40 ml of milk was collected, and sent to the Milk Quality Laboratory (Lab UFMG) to evaluate the amount of nitrogen present in the milk. Nitrogen efficiency was calculated as the ratio of total milk Nitrogen (g day -1 ) and the total Nitrogen intake (g day -1 ). Data were submitted to analysis of variance, F test (α<0.05), decomposed into orthogonal contrasts and polynomial regression. N-efficiency, in the order of CG inclusions, was 18.03 %; 17.14 %; 19.81 %; 18.74 %. There was a linear increase in N-efficiency (α<0.01) in function of the CG supplementation. Probably the CG provided a better use of ruminal nitrogen by the macrobiotic, with less losses of ammonia. The nitrogen efficiency in production of milk can be improved by supplementing crude glycerin diluted in water for dairy sheep. Keywords: biofuels, ewe, glycerol, protein. Acknowledgments: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do estado de Minas Gerais (FAPEMIG) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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Page 1: Nitrogen efficiency of dairy sheep supplemented with crude ... · Nitrogen efficiency of dairy sheep supplemented with ... individual sample of 40 ml of milk was ... (Lab UFMG) to

Nitrogen efficiency of dairy sheep supplemented with crude glycerin diluted in water*

Natália C. Alves1**, José A. Junior2, Felipe S. Santos2, Hemilly C. M. Sá3, Iran Borges2, Gilberto

L. Macedo4, Tassia L. T. M.2, Jizênio A. S. Passos1, Juliana A. Favato1, Celso H. F. Paiva1

*Part of master thesis of the third author 1Undergraduate student Veterinary School of Federal

University of Minas Gerais; 2Animal Science Department, Veterinary School of Federal

University of Minas Gerais;3Animal Science Department, Federal University of Sergipe; 4Animal Science Department, Federal University of Uberlândia;

**Federal University of Minas Gerais, Av. Presidente Carlos Luz, 4466, São Francisco, CEP

31250-810, Belo Horizonte, MG, Brazil; [email protected]

The Crude Glycerin (CG) expressed a potential for use in diet of dairy ruminants. Recent

renewable energy use policies have promoted a CG surplus. This byproduct can be applied to

ruminant nutrition as a cheap source of energetic food, with easily storage and delivery. The use

of CG can provide balance between energy and protein, according to ruminal degradation kinetics,

that results in maximum microbial growth, in lower urinary losses of energy and nitrogen, and

optimization of dietary nitrogen use for casein synthesis. The objective of this study was to

evaluate the effects of the inclusion of crude glycerin diluted in water for dairy sheep on nitrogen

(N) efficiency for milk yield. Were used 20 Lacaune x East Friesian lactating dairy ewes, between

three and four years’ age, with the average weight of 62.68 ± 5.95kg. The animals remained in

individual pen with free access to water, and concentrate and roughage were provided separately.

The sheep were distributed in a completely randomized design, in four treatments differed as to

supplementary CG intake diluted in water: zero, 1.5%, 3.0% and 4.5% of dry matter intake. Ewes

were Machine milked once a day at 15:00. The lactation persisted to 17 weeks. The diets were

formulated to meet the nutritional requirements according to the NRC (2007). Weekly an

individual sample of 40 ml of milk was collected, and sent to the Milk Quality Laboratory (Lab

UFMG) to evaluate the amount of nitrogen present in the milk. Nitrogen efficiency was calculated

as the ratio of total milk Nitrogen (g day -1) and the total Nitrogen intake (g day -1). Data were

submitted to analysis of variance, F test (α<0.05), decomposed into orthogonal contrasts and

polynomial regression. N-efficiency, in the order of CG inclusions, was 18.03 %; 17.14 %; 19.81

%; 18.74 %. There was a linear increase in N-efficiency (α<0.01) in function of the CG

supplementation. Probably the CG provided a better use of ruminal nitrogen by the macrobiotic,

with less losses of ammonia. The nitrogen efficiency in production of milk can be improved by

supplementing crude glycerin diluted in water for dairy sheep.

Keywords: biofuels, ewe, glycerol, protein.

Acknowledgments: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq),

Fundação de Amparo à Pesquisa do estado de Minas Gerais (FAPEMIG) and Coordenação de

Aperfeiçoamento de Pessoal de Nível Superior (CAPES).