0.1100

0.2100

0.3100

0.4100

0.5100

0.6100

0.7100

350 450 550 650 750 850 950 1050 1150 1250 1350

Tunable Negative Index Lens• Focusing by negative index lens

– Si-based photonic crystal with negative index– Focusing of light due to negative refraction

• Mechanically Tunable Photonic Crystal– New pathway to tunability

Program Goal• Achieve on-chip detection of cancer with high

specificity and high sensitivity• Interdisciplinary program that combines

nanophotonics, nano-elecrtro-mechanical systems (NEMS), microfluidics, and cancer biology

• Thrust areas– Tunable negative index lens– On-chip system integration– Nanoprobe-based cancer detection

• Team– Won Park (University of Colorado at Boulder)– Lynne Bemis and William Robinson (UCDHSC)– J.-B. Lee (University of Texas at Dallas)– John Moreland (NIST-Boulder)– Geoff Lindsay (NAVAIR, China Lake, CA)– Zyvex Corp. and MicroFab Technologies, Inc.

On-Chip System Integration• Microfluidic channel

• Light source and detector

Program Overview

Nanoprobe-Based Cancer Detection• Metal nanoparticles and nanoshells

– Controlled plasmon resonance

• DNA conjugation andcancer detection

Broader Impacts

• Education and Human Resources Development– New course development

• Promote underrepresented minority participation– Genetic Education for Native Americans (GENA)

• Contribute to the existing research infrastructure at the University of Colorado Cancer Center and Nanotech Initiative at UTD

• Interact with Photonics/Biophotonics Industry in Colorado and Telecom Industry in North Texas

• Collaborations with federal research institutions• Dissemination of research and outreach

– Broomfield Library (Broomfield, CO)– The Science Place (Dallas, TX) – Web site: http://onchip.colorado.edu

• Benefits to society at large– Contribute to the nation’s fight against cancer

FundamentalNanoscale

Phenomena &Processes

NanophotonicCrystal

(UCB,UTD)

NEMSActuators

(UTD)

Nano-bio-photonics

(UCB,UCDHSC)

NanoscaleDevices

Dynamically TunableNegative Index Lens

(UCB,UTD)

NanoscaleSystems &

HierarchicalNanomanufacturing

Heterogeneously Integrated On-Chip Spectroscopy System(UCB,UTD,UCDHSC)

Micro/macro:Micro/macro: Diced light sources & detectors, microfluidicchannel, MEMS springs and structures for self-alignment

Nano:Nano: Tunable negative index lens, NEMS, nanoprobes

Broader Impact/Outreach

BroomfieldLibrary,

Broomfield,Colorado

The SciencePlace,

Dallas, TX

Web sites,Journal &

conferencepublications

UCB – ParkUTD – Lee

NISTNAVAIRZyvex MicroFab

CancerBiology

(UCDHSC)

UCDHSC –Bemis, Robinson

Diversity:GENA,MAP

ResearchTeam

PC

Si WG

Si Deflection BlockLight

Refracted Light

Stress Stress

Light

Refracted Light

Stress Stress

experimentexperimentMieMie theorytheory

Gold

nanoshell

BRAF in patient sample

Mut-BRAF in patient sample

Nanoprobe 1

-S-S-C18-C18-primer-(BRAF mutation) (BRAF DNA)-primer-C18-C18-S-S-

Nanoprobe 2

-S-S-C18-C18-primer-(BRAF) (BRAF DNA)-primer-C18-C18-S-S-

Nanoprobe BRAF

Nanoprobe BRAF

Gold nanoshell

Gold nanoshell

Gold nanoshell

Gold nanoshell

BRAF in patient sampleBRAF in patient sample

Mut-BRAF in patient sampleMut-BRAF in patient sample

Nanoprobe 1

-S-S-C18-C18-primer-(BRAF mutation) (BRAF DNA)-primer-C18-C18-S-S-

Nanoprobe 2

-S-S-C18-C18-primer-(BRAF) (BRAF DNA)-primer-C18-C18-S-S-

Nanoprobe BRAF

Nanoprobe BRAF

Gold nanoshell

Gold nanoshell

Gold nanoshell

wildtype

partial mutant

mutant

Mutant/hetero

A

B

C

D

• Target genes

InputWaveguide

NIM PC Output

TM

InputWaveguide

NIM PC Output

TMTM

Fluidic inlets

Mixing chamber

Fluidic outlet

Fluidic inlets

Mixing chamber

Fluidic outlet

PDMS microfluidic channelFully packaged microfluidic system with a ferromagnetic mixer

Microlens on movable microshuttle

Integrated VCSEL array

Lasing from integrated

VCSEL

NIRT: Active Nanostructure Enabled On-Chip Spectroscopy System for Cancer DetectionUniversity of Colorado at Boulder, University of Colorado at Denver and Health Sciences Center,

and University of Texas at Dallas (http://onchip.colorado.edu)

λ = 1560 nm