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Nicotiana benthamiana. Malva parviflora. AY591807 Abutilon andrewsianum. EF219369 A. grandifolium. EF219363 A. eremitopetalum. 760. 774. EF219364 A. eremitopetalum. 521. EF219365 A. menziesii. 999. EF219367 A. sandwicense. 1000. EF219366 A. sandwicense. - PowerPoint PPT Presentation
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Nicotiana benthamiana
Malva parviflora
AY591807 Abutilon andrewsianum EF219369 A. grandifolium
EF219363 A. eremitopetalum EF219364 A. eremitopetalum EF219365 A. menziesii
EF219367 A. sandwicense EF219366 A. sandwicense
EF219368 A. incanum AY591808 A. sp. La Duke s.n. AY863058 Typhonium divaricatum AY863059 A. indicum DQ006017 A. theophrasti DQ287984 A. theophrasti AY591815 Bastardiopsis densiflora
AY591812 Bakeridesia gloriosa AY591859 B. gaumeri
A. megapotamicum AY591818 Briquetia sonorae
AY591817 Billieturnera helleri AY591851 Robinsonella lindeniana AY591824 Fryxellia pygmaea
GQ478107 Sida fallax DQ006018 S. spinosa
RCA21 PCR1RCA21 PCR2
AY591855 Tetrasida weberbaueri EF679734 Althaea armeniaca EF679733 Althaea officinalis EF419541 Althaea cannabina EF419540 Althaea cannabina EF419539 Althaea cannabina GU166763 Alcea hohenackeri GU166772 Alcea rhyticarpa GU166759 Alcea glabrata
EF419547 Anisodontea malvastroides EF635464 Hoheria populnea HM348802 Plagianthus divaricatus EF152583 Cristaria insularis
1000
681
999
994
1000
1000
1000
1000
999
995 977632
1000
652
533
826 674
726 1000
721
10001000
760774
999
1000
521
0.01
a N. benthamiana
HM437879.1
HM437882.1
M. parviflora
HM446943.1
GU135393.1
GQ982301.1
AY371673.1
AY371675.1
FJ038996.1
RCA21 PCR1RCA21 PCR2
DQ006201.1 S. spinosa
DQ006200.1 A. theophrasti
GQ435057.1
HQ415485.1
AB617863.1
AB617862.1
AB452562.1
AB452563.1
1000
774
1000
537
980
773
523
1000
1000
1000
797
1000
635
769
1000
0.01
b
Nicotiana benthamiana
DQ006130 Justicia americana
RCA22 Peristrophe spp. (Cuba)
Peristrophe speciosa (Potsdam)
JF708219 Peristrophe japonica
GQ435144 Rhinacanthus nasutus
EU725798 Rhinacanthus nasutus
EU725795 Rhinacanthus nasutus
GQ995666 Dyschoriste albiflora
GQ995627 Ruellia ciliatiflora
GQ995634 R. paniculata
GQ995631 R. foetida
GQ995657 R. pringlei
GQ995658 R. pringlei
GQ995665 R. speciosa
GQ995655 R. laslobasensis
GQ995629 R. eumorphantha
870
587
824
1000
955
1000
951
1000
1000
996
598
0.02
c
Cubana_1302_Suppl. Figure 1
CCGG GATC
M 15 16 17 18 19 20 22 21 23 24 25 26 27 15 16 17 18 19 20 22 21 23 24 25 26 27
1252
698 -681
348-371
256-293
2632
ud
Calculated ethidium bromide invertCalculated ethidium bromide invert
192119211833183320172017
997997
18191819
16271627
25932593
12341234
17381738
2699269926452645 25932593
165016501592159214951495
18761876
689689
13251325
833833
23362336
10391039
13331333
19681968
514514
16751675
968968 985985
693693
645645
968968
666666
20502050
956956
12261226
17121712
7878
979979
508508
607607
852852
613613597597
728728
556556
18471847
892892934934
16271627
514514
277277
415415
828828
584584
435435
678678
476476
16031603
833833
693693
10141014
468468
249249264264
678678
492492
420420
590590
370370
12341234
761761
372372
872872
376376
139139
638638
401401
268268 262262
230230
857857
645645
284284
787787
9292
457457
353353
186186
162162
766766
549549
266266
704704
262262
162162
132132
663663
435435
648648
194194
613613
418418
590590
153153
514514
353353
406406
439439
245245
286286
396396
372372
242242
15811581
10031003
787787736736
10031003
16751675
991991
14061406
10081008 968968
10711071
704704
815815
940940
655655
587587
872872
779779
537537
903903 908908
678678641641
704704
435435
531531
824824
613613
478478
716716
862862
531531540540 537537
401401
478478
670670
473473
437437
620620
810810
406406
353353
481481
332332308308
540540
353353
250250
559559
720720
330330
288288
468468
273273293293
465465
244244
359359
659659
259259
200200
432432
202202
235235
353353
224224
185185
641641
229229
173173
232232225225
293293
181181
349
546
1829
676
105
5A
880826
9
349
546
1833
675
105
4A
829
279
221
192188
9
350
2A
688
609
494
392
668
435
331
215195188163154134
72-9
347
11A
1803
770
43,9
965
613
466
216
Predicted DNA APredicted DNA A3A
599,593
431417
267
178
147
535497
226215,205
174105105
88-6
A1/A2
1030
535
902
476
226215,213,205
175
10593,87,76
61-6
769
431
251,258
348
Cubana_1302_Suppl. Figure 2
CCGG GATC
M 15 16 17 18 19 20 22 21 23 24 25 26 27 15 16 17 18 19 20 22 21 23 24 25 26 27
1252
698 -681
348-371
256-293
2632
ud
Calculated ethidium bromide invertCalculated ethidium bromide invert
192119211833183320172017
997997
18191819
16271627
25932593
12341234
17381738
2699269926452645 25932593
165016501592159214951495
18761876
689689
13251325
833833
23362336
10391039
13331333
19681968
514514
16751675
968968 985985
693693
645645
968968
666666
20502050
956956
12261226
17121712
7878
979979
508508
607607
852852
613613597597
728728
556556
18471847
892892934934
16271627
514514
277277
415415
828828
584584
435435
678678
476476
16031603
833833
693693
10141014
468468
249249264264
678678
492492
420420
590590
370370
12341234
761761
372372
872872
376376
139139
638638
401401
268268 262262
230230
857857
645645
284284
787787
9292
457457
353353
186186
162162
766766
549549
266266
704704
262262
162162
132132
663663
435435
648648
194194
613613
418418
590590
153153
514514
353353
406406
439439
245245
286286
396396
372372
242242
15811581
10031003
787787736736
10031003
16751675
991991
14061406
10081008 968968
10711071
704704
815815
940940
655655
587587
872872
779779
537537
903903 908908
678678641641
704704
435435
531531
824824
613613
478478
716716
862862
531531540540 537537
401401
478478
670670
473473
437437
620620
810810
406406
353353
481481
332332308308
540540
353353
250250
559559
720720
330330
288288
468468
273273293293
465465
244244
359359
659659
259259
200200
432432
202202
235235
353353
224224
185185
641641
229229
173173
232232225225
293293
181181
B1
1248
653
535
152
1383
474
183
123
108
76-6
11B
2598
752
621
238219
136128114111108
76-6
10B
1610
679
256
695
456423
300
182
126122117108
16
5B
946
616
1547
653
167
105
65-14
1894
585
152
4B
790
403
272
231204
143
111
76-4
3B
984
676658
256
748
590
311
232
182
126
69-6
Predicted DNA BPredicted DNA B
Cubana_1302_Suppl. Figure 3
1820
1336
417
1752
1446
667 682
613
548
489
405
353
593
440417
177
1765
678
1739
754
1005
889
763
671
445420
353
253
1688
678
168
1582
785
682
512
400
286
1427
976
620
515
1176
671
517
826
554
362
290
224
194
965
557
494
365
229
189
671
340
224
196
551538
235215
845
551
365
294
229
197
1005
634
573
497
224
551
220
921
682
563
365
116
982937874
821
746
634
542554
451
381
340
298
66
CCGG GATC
M 15 16 17 18 19 20 22 21 23 24 25 26 27 15 16 17 18 19 20 22 21 23 24 25 26 27
Calculated DNA A hybridizationCalculated DNA A hybridization
1252
698 -681
348-371
256-293
2632
ud
349
546
1829
676
105
5A
880826
9
349
546
1833
675
105
4A
829
279
221
192188
9
347
11A
1803
770
43,9
965
613
466
216
Predicted DNA APredicted DNA A
350
2A
688
609
494
392
668
435
331
215195188
163154134
72-9
3A
599,593
431417
267
178
147
535497
226215,205
174
105105
88-6
A1/A2
1030
535
902
476
226215,213,205
175
10593,87,76
61-6
769
431
251,258
348
Cubana_1302_Suppl. Figure 4
CCGG GATC
M 15 16 17 18 19 20 22 21 23 24 25 26 27 15 16 17 18 19 20 22 21 23 24 25 26 27
919
1827
939
12311154
1245
2359
796
899
1663
660
934
637
1174
444
583
140
627
256
142
904
1299
248 258
139
678
565
375
525
272250
1502
970
784748
975
1563
1013970
1071
654
442 449
601
876
1390
725
817
748
413
490 487
627
756
432
209
319
577
660
259
187
Calculated DNA B hybridizationCalculated DNA B hybridization
698 -681
348-371
256-293
B1
1248
653
535
152
1383
474
183
123
10876-6
11B
2598
752
621
238
219
136128114111108
76-6
10B
1610
679
256
695
456
423
300
182
126122117108
16
5B
946
616
1547
653
167
10565-14
1894
585
152
4B
790
403
272
231
204
143
11176-4
3B
984
676658
256
748
590
311
232
182
126
69-6
Predicted DNA BPredicted DNA B
Cubana_1302_Suppl. Figure 5
