NHMFL User Committee MeetingNMR/MRI Subgroup

October 2nd 2009

Update from

The Advanced Magnetic Resonance Imaging & Spectroscopy Program

at the University of Floridaand

The NMR Spectroscopy & Imaging Program

at Florida State University

NHMFL User Committee MeetingNMR/MRI Subgroup

October 2nd 2009

Agenda11:00 NMR/MRI Program Overviews,

Tim Cross Joanna Long

11:45 Lunch

12:30 Tallahassee Presentations Fengli ZhangSam GrantZhehong GanBill Brey

14:00 HTS NMR Magnet Prospects

14:30 Coffee Break

14:45 Gainesville PresentationsTom MareciSteve BlackbandAaron Dossey

16:15 Discussion of Priorities

User Statistics from NMR/MRI NHMFL Activities

• • ICR, NMR, EMR, AMRIS and Geochemistry ICR, NMR, EMR, AMRIS and Geochemistry provide the majority of the provide the majority of the chemical & biological research user programschemical & biological research user programs

and represent 25-45% of the Magnet Lab user productivityand represent 25-45% of the Magnet Lab user productivity

»» Research Reports (2005-2008):»» Research Reports (2005-2008):NMR/MRI (UF & FSU)NMR/MRI (UF & FSU) 195195ICRICR 8181EMREMR 134 total ~1/2 are chem/bio134 total ~1/2 are chem/bio 6767GeochemistryGeochemistry 7575total total - - represents 26% of all NHMFL reportsrepresents 26% of all NHMFL reports 418418

»» Research Reports for 2008: »» Research Reports for 2008: NMR/MRI (UF & FSU)NMR/MRI (UF & FSU) 8585ICRICR 1515EMREMR 39 total ~1/2 are chem/bio39 total ~1/2 are chem/bio 2020GeochemistryGeochemistry 2222total - represents 38% of all NHMFL reports total - represents 38% of all NHMFL reports 144144

User Statistics from Chem/Bio NHMFL Activities

• • ICR, NMR, EMR, AMRIS and GeochemistryICR, NMR, EMR, AMRIS and Geochemistry

»» Users for 2008:»» Users for 2008:NMR/MRI (UF & FSU)NMR/MRI (UF & FSU) 257257ICRICR 9797EMREMR 99 total ~1/2 are chem/bio99 total ~1/2 are chem/bio 5050GeochemistryGeochemistry 4646total total represents 46% of all NHMFL usersrepresents 46% of all NHMFL users 450450

»» Publications for 2008: »» Publications for 2008: NMR/MRI (UF & FSU)NMR/MRI (UF & FSU) 7777ICRICR 1717EMREMR 47 total ~1/2 are chem/bio47 total ~1/2 are chem/bio 2424GeochemistryGeochemistry 99total total represents 30% of all NHMFL pubs represents 30% of all NHMFL pubs 127127represents 46% of all NHMFL User Facility Pubsrepresents 46% of all NHMFL User Facility Pubs

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UF/FSU NMR/MRI News and Collaborative Efforts

• Art Edison agreed to serve the NHMFL as Associate Director for Chemistry and Biochemistry

• This summer he led an effort to write a proposal for a 950 MHz spectrometer focusing on 13C direct detection at high fields with both cryo and HTS probes - the latter to be jointly developed by Bill Brey and

Agilent, building on the previous collaboration between Bill Brey and Rich Withers, formerly of Bruker.

• Funding for a new HTS probe has been achieved represents a similar collaboration to that described above, but here for a higher mass sensitivity probe at 1.5 mm with enhanced filling factor.

• The External Advisory Committee met in August and recommended that the vacant chief scientist position be filled with a Chem Bio researcher with major NIH funding.

FSU NMR Spectroscopy and Imaging Mission

»» Multi-Frequency RF Probe Development»» Low-E RF Probes»» HTS Probe Development»» Additional Probe Hardware for Unique Capabilities»» Magnetic Field Stabilization Hardware»» Theoretical Spin Physics»» Novel Pulse Sequence Development»» Web-based Metabolomics Tools»» Experimental Protocols»» Technology and Science Drivers for Novel and

Ultra High Field Magnets

• User Driven Unique Technology Development for High Field NMR Spectroscopy and Imaging

Bo

• User Driven Development of Unique Applications for High Field NMR Spectroscopy and Imaging

»» Inorganic Chemistry and Materials Chemistry»» Biophysics and Biochemistry»» Drug Screening and Metabolomics»» Anatomy and Biomedical Science»» Spin Physics»» Macromolecular Dynamics and Structure

FSU Facility Highlights from ‘08

• Installed Bruker Biospin Avance III console for the 900 and the Avance I 900 console will replace the current DRX console on the WB600 - this was part of a $3.2M contract for NMR instrumentation to FSU. For the 900 new console signal generation units and receivers, but with the previous RF power and gradient amplifiers as well as previous preamplifiers. Up to date software for the 900 and WB600.

» state of the art capability for solids and imaging on the 900 and much improved capability for the WB600 - both WB 600 instruments will have Avance I consoles

• New WB 500 - 50% for Prof. Anant Paravastu and 50% for the NMR User facility - primarily for MAS solids. A 1.3 mm 60kHz MAS probe will be delivered with the instrument

2009 Planned Proposals for NMR User Program

• Proposal to NIBIB for a BRP in support of solid state NMR and and Na+ imaging: “Novel Technology for Biomedical Research in Ultra-High Magnetic Fields” - letter requesting permission to submit proposal will be sent out next week. Proposal to be submitted 2/09. PI will be Cross

• S10 Proposal to the NCRR ‘High-End NMR’ program for a 900 MHz console - to be submitted 1/09. PI will be Sam Grant

• HTS 3mm probe for structural biology - to be submitted in late ‘09. PI will be Brüschweiler

Proposal after 12 months of negotiations with NIBIB was refused for lack of interest in High Field Imaging and SSNMR.

Plan involved lease/purchase arrangement and University refused to permit us to submit it - FSU funded $2.5M and the NHMFL provided $0.7M for console upgrades.

Proposal led by Art Edison for 950 MHz with 3 mm HTS probe submitted for High-End Instrumentation to NCRR

Follow up on plans presented last year

2009 Planned Proposals for NMR User Program

• NIH proposal (mechanism undefined) for design/ construction of a 30 T high homogeneity HTS magnet - to be submitted in late ‘09

• NSF MRI proposal for the 36 T SCH console - to be submitted 1/10

Proposal led by Bill Brey for SCH console submitted as an MRI Proposal to NSF

After extensive deliberation it appears that we should obtain more preliminary HTS magnet results - 32 T HTS cold bore magnet for physics has been funded by NSF MRI.

Follow up on plans presented last year

• $9M Program Project Grant funded with UCSD, U. Alabama, Burnham Inst., & Harvard - validation of membrane protein drug targets- search for ligands that bind (initial drug screening)- structural characterization of 10 TB membrane proteins- development of triple resonance low-E probes for high fields- development of solid state NMR drug screening probes

Additional funding efforts

Moving Forward - Continuing Facility Enhancement in Tallahassee - setting priorities

• Last few years we pointed out that much of our instrumentation is seriously aging, some progress has been made.

» UWB 900 - new console delivered in ‘09

» VNB 830 - needs a high power RF amplifier - will order Fall ‘09

» SB 720 - replace with a solution NMR centerpiece - 950 Proposal submitted

» SB 600 - replace the 10 yr. old console - (along with 950 purchase)

» WB 600a - an Avance I system - not currently a top priority

» WB 600b - to be upgraded to Avance I in ‘09

» SB 500 w/cryo - receives little demand

» (WB 500) - to be delivered in ‘09

» WB 400 - an Avance I console

» SB 300 - staging instrument for the Keck magnet with TecMag console

» WB 300 - important staging instrument - 14 year old DRX console

» 36 T SCH - proposal submitted summer ‘09

Moving Forward - Continuing Facility Enhancement in Tallahassee - setting priorities - May ‘09 report to GSB

Emergency Needs (list one item)Replacement of the 720 MHz instrument (950 MHz proposal - no resources if this is

not funded)Top Priority Needs (list three items to maintain existing user program without growth)

Spectroscopist scholar scientist for the 950 MHz instrument if fundedSeries Connected Hybrid Console if not funded (MRI R2 proposal - no resources if

this is not funded)Expense and Equipment for RF development and maintenance

Middle Priority Needs (list three items - necessary growth to maintain premier position)Jason Kitchen - RF engineer - temporarily funded on NMR account without budgetSeries Connected Hybrid Scholar ScientistConsole for 600 MHz instrument (should be done in concert with 720 replacement)

Emergency Needs (list five items - visionary growth in user program or strategic planning)Development of DNP CapabilitiesAnimal Resource Technician (2 years of support from Grant’s start up)Software Application Engineer to facilitate software disbursement Replacement of two WB600 and 830 MHz consoles

We would like to have your advice on these or other priorities