aati-us.comR

NGS AnalysisFragment AnalyzerTM Automated CE System

Streamline workfl ow by rapidly quantifying and qualifying fragments for NGS

During NGS library preparati on, initi al DNA samples may be fragmented/sheared, tailed, ligated, size selected, captured, released, and/or amplifi ed. Accurate quanti fi cati on and qualifi cati on of DNA at crucial quality control checkpoints during NGS library constructi on to ensure successful sequencing. Conventi onal fragment analysis with lab-on-chip methods generates bott lenecks for high-throughput NGS environments.

Hundreds of NGS laboratories have adopted a bett er quality control strategy. The Fragment Analyzer Automated CE System provides NGS labs with a variable throughput method, enabling dozens to thousands of samples to be analyzed per day. The Fragment Analyzer used in conjuncti on with the DNF-473 Standard Sensiti vity NGS Fragment Analysis Kit and the DNF-474 High Sensiti vity NGS Fragment Analysis Kit provides confi dent sizing and quanti fi cati on data for all short-read sequencing NGS libraries.

Sample ID Trace Range ng/µL % Total nmole/L Avg. Size % CVNGS 1 Black 30 – 1,500 bp 3.8759 99.6 17.6542 361 50.54NGS 2 Red 30 – 1,500 bp 1.7835 99.2 8.2248 357 51.42NGS 3 Blue 30 – 1,500 bp 0.9293 98.1 4.1529 368 51.64NGS 4 Orange 30 – 1,500 bp 0.4368 96.2 1.9389 371 52.34

Separati on and quanti fi cati on of NGS libraries using the DNF-474 High Sensiti vity NGS Fragment Analysis Kit. Capillary electrophoresis was performed on a Fragment Analyzer Automated CE System equipped with a Short Capillary Array (33-55). Post-electrophoreti c analysis was completed with the proprietary PROSize Data Analysis Soft ware. Smear Analysis was performed with a smear range of 30 bp to 1,500 bp, reporti ng: concentrati on, % of total, molarity, average size, and % CV.

Copyright ® 2017 Advanced Analyti cal Technologies, Inc. All Rights Reserved. Reproducti on, adaptati on or translati on without prior writt en permission is prohibited except as allowed under copyright laws. Fragment Analyzer™ is a trademark of Advanced Analyti cal Technologies, Inc. PROSize® is a registered trademark of Advanced Analyti cal Technologies, Inc.

2450 SE Oak Tree Ct.Ankeny, IA 50021Phone: + 1-515-964-8500Fax: + 1-515-964-7377E-mail: sales@aati -us.comwww.aati -us.com

Hans-Bunte-Str. 5/169123 HeidelbergPhone: + 49 6221 868058-20Fax: + 49 6221 868058-99E-mail: sales@aati -de.comwww.aati -de.com

Bio-Cluster Genopole - Campus 15 rue Henri Desbruerès91030 Every cedexPhone: + 33 1 78 05 47 22E-mail: sales@aati -fr.comwww.aati -fr.com

Advanced Analyti cal Technologies, Inc.

4 Rankin House, Murdoch CourtKnowlhill, Milton Keynes K5 8HLUnited Kingdom

E-mail: sales@aati -uk.comwww.aati -uk.com

aati-us.com

R

Fragment Analyzer™ Automated CE System

Process Comparison MethodsLab-on-Chip Method1. Prepare gel/dye prior to use, store in dark at 4°C2. Equilibrate gel to room temperature for 30 minutes3. Remove chip from bag4. Pipett e 9 µL of gel onto chip5. Assemble chip priming stati on and depress syringe

plunger, wait exactly 60 seconds6. Wait an additi onal 5 seconds7. Remove chip priming stati on 8. Pipett e 9 µL of gel into 2 more wells on chip9. Pipett e 5 µL marker into each sample well used10. Pipett e 1 µL of ladder into one well11. Pipett e 1 µL of sample into each well on the chip12. Place loaded chip onto vortexer13. Vortex for 60 seconds14. Load chip and begin run within 5 minutes15. Start method16. Analyze results17. Generate report

Fragment Analyzer Method1. Prepare gel-dye and store at room temperature2. Prepare inlet buff er tray3. Prepare rinse buff er tray4. Dilute 2 µL samples and ladder with diluent

marker into 96-well sample plate5. Place sample plate onto instrument6. Start method7. Analyze results8. Generate report

Features and Benefi tsSimplifi ed Sample HandlingRequires a single diluti on step into a 96-well plate

No Chip LoadingSeparati on gel is automati cally loaded into capillaries prior to each run

High SensitivityQuanti fy library smears as low as 50 pg/µL

Short Run Times Complete analysis as quickly as 40 minutes

Multi-Plate CapacityHolds up to 288 samples in three, 96 well plates, sample rows/plates can be analyzed in user-defi ned order

Long Reagent Shelf-LifeHolding reagents at room temperature for extended periods, allows for quick access and reduced instrument preparati on ti me

Suitable for All NGS PlatformsProvides quality and quanti ty analysis for the major NGS platf orms

Powerful Data Analysis SoftwarePROSize enables automated baseline selecti on, Smear Analysis, “Apply to All” feature, and customized report formatti ng for size distributi on and concentrati on measurements

NGS Analysis Fragment AnalyzerTM Automated CE System

MRK-BRNGS-0002-0617