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NEXTFLEX® RAPID XP DNA-SEQ KIT N O VA - 5 1 4 9 - 0 2 - P R O D U C T I N T R O D U C T I O N
Commercial launch on October 12, 2018 perkinelmer-appliedgenomics.com/rapid-xp
Catalog #NOVA-5149-02 (48 reactions)
List price USD1200
Robust DNA-seq kit that includes
enzymatic fragmentation to obtain
sequence-ready libraries from genomic
DNA using a streamlined protocol
NEXTFLEX® RAPID XP DNA-SEQ KIT XP STANDS FOR EXTRA PERFORMANCE
Reliable
Convenient
Flexible
Consistent fragmentation, library yields, and high-quality
sequencing data
All-inclusive kit that has been streamlined and automated
on Sciclone® and Zephyr® NGS workstations
Use with NEXTFLEX® color-balanced barcodes for
multiplexing up to 384 samples and PCR-free workflows
NEXTFLEX® RAPID XP DNA-SEQ KIT VALUE PROPOSITION
NEXTFLEX® RAPID XP DNA-SEQ KIT WORKFLOW
Manual (1.5 to 2.5 hours) Sciclone® workstation
Zephyr® workstation
NEXTFLEX® RAPID XP DNA-SEQ KIT CONSISTENT ENZYMATIC FRAGMENTATION
Figure 1: Reliable DNA enzymatic fragmentation with NEXTFLEX® Rapid XP
DNA-seq kit. Each dot indicates the library peak size. DNA is uniformly and
reproducibly fragmented into smaller sizes using the NEXTFLEX® Rapid XP DNA-
seq kit (457 ± 12 bp, c.v.=3 %, n=18). In comparison, the DNA libraries obtained
using Competitor K’s kit was non-uniform and variable indicated by the diffuse
pattern observed in the plot (730 ± 119 bp, c.v.=16 %, n=17). Libraries were
prepared from 100 ng of gDNA from 5 different commercially available sources
with 8 minutes of fragmentation and 5 cycles of PCR.
NEXTFLEX® RAPID XP DNA-SEQ KIT HIGH LIBRARY YIELD
Figure 2: High yield obtained for libraries prepared
using the NEXTFLEX® Rapid XP DNA-seq
kit. Libraries were prepared using both NEXTFLEX®
Rapid XP DNA-seq kit and Competitor K’s kit. Libraries
were prepared from 100 ng of DNA with 8 minutes of
fragmentation with 5 PCR cycles.
0
50
100
150
200
250
Human male Human mixed Jurkat Hela MCF7 Placenta
Yiel
d (
nM
)
NEXTFLEX® Competitor K
NEXTFLEX® RAPID XP DNA-SEQ KIT LOW GC BIAS
Figure 3. Libraries prepared with the
NEXTFLEX® Rapid XP DNA-seq kit show less or
comparable GC-bias than libraries prepared
with Competitor K’s kit. Picard GC bias analysis,
which show read coverage over the indicated
genome with respect to GC content, is shown as a
fraction of normalized coverage (blue) calculated in
100 bp windows (red) and plotted against the left y-
axis. Mean base quality at each window (green) is
calculated and plotted against the right y-axis.
Saccharomyces cerevisiae
NEXTFLEX®
Competitor K
Escherichia coli Bordetella pertussis
NEXTFLEX® RAPID XP DNA-SEQ KIT HIGH GENOME COVERAGE
Figure 4. Libraries prepared with the NEXTFLEX® Rapid XP DNA-seq kit show higher or comparable genome coverage
compared to libraries prepared using Competitor K’s kit. The percentage of genome bases covered at a minimum depth of 1X, 5X,
and 10X of S. cerevisiae, E. coli, and B. pertussis.
70 %
75 %
80 %
85 %
90 %
95 %
100 %
1 X 5 X 10 X
S. cerevisiae
NEXTFLEX® Competitor K
92 %
93 %
93 %
94 %
94 %
95 %
95 %
96 %
96 %
97 %
1 X 5 X 10 X
E. coli
NEXTFLEX® Competitor K
92 %
93 %
93 %
94 %
94 %
95 %
1 X 5 X 10 X
B. pertussis
NEXTFLEX® Competitor K
NEXTFLEX® RAPID XP DNA-SEQ KIT HIGH MAPPING RATE AND LOW DUPLICATION RATE
Figure 5. Libraries prepared with the NEXTFLEX® Rapid XP DNA-seq kit resulted in better or comparable data with respect to
mapping rate and duplication rate, compared to libraries prepared using Competitor K’s kit. (A) Mapping rates represented as the
percentage of reads which aligned to the indicated genome. (B) Duplication rates represented as the percentage of reads predicted to be PCR
duplicates by Picard MarkDuplicates.
0 %
10 %
20 %
30 %
40 %
50 %
60 %
70 %
80 %
90 %
100 %
S. cerevisiae E. coli B. pertussis
Mapping Rate
NEXTFLEX® Competitor K
0 %
1 %
2 %
3 %
4 %
5 %
6 %
7 %
8 %
S. cerevisiae E. coli B. pertussis
Duplication Rate
NEXTFLEX® Competitor K
NEXTFLEX® RAPID XP DNA-SEQ KIT POSITIVE EARLY ACCESS DATA
Successful displacement of incumbent chemistry
(Agilent® SureSelectXT®)
Compatibility with hybrid capture and multiple sample
types (saliva, whole blood, DBS, swab)
Fewer PCR cycles (less bias)
Robust Library yields reported from 50ng gDNA input
Site 1: Perkin Elmer Genomics
Site 2: Anonymous (customer site)
NEXTFLEX® RAPID XP DNA-SEQ KIT POSITIVE EARLY ACCESS DATA
Fewer steps => time and consumable savings Faster and more consistent fragmentation
Site 3: Perkin Elmer Health Sciences Australia
NEXTFLEX® Competitor K
12 minutes fragmentation 15 minutes fragmentation
XP produces tighter & more
consistent size distribution
compared with KAPA Hyperplus
with less incubation time.
Combined fragmentation and
ERAT reduces hands on time.
EXTERNAL POSITIONING: KAPA®
You Customer
Kapa® HyperPlus™ Library Prep Kit w amp NEXTFLEX® Rapid XP DNA-Seq Kit
$32 Price Per Sample (no barcodes, only kit) $25
No (~$3-$5 per prep) Beads included? Yes (No additional cost)
96 How many barcodes are available? 384
Two steps Fragmentation and end-repair/adenylation One step
50 ng Minimum Input amount complex genomes 1 ng
Variable (see supporting data in the appendix) Fragmentation efficiency Consistent (see data in the deck)
Reagent One-stop shop Automation + Reagent
I have been using Kapa
kits and am generally
happy with it.
I understand. Kapa is a widely-used kit. However, we have a new
compelling alternative that can save you money, time, and trouble.
Save >$10 prep sample (at list price)
Save on sequencing by higher multiplexing
More consistent enzymatic fragmentation
Input for complex genomes down to 1ng
Single point of contact for all library prep support
EXTERNAL POSITIONING: NEB®
You Customer
NEBNext® Ultra™ II FS DNA Library Prep with Sample Purification Beads NEXTFLEX® Rapid XP DNA-Seq Kit
$27.5 Price Per Sample (no barcodes, only kit) $25
96 How many barcodes are available? 384
No, indexed primers also increase index hopping PCR-free workflow Yes
Variable (see supporting data in the apendix) Operator repeatability Consistent
Reagent One-stop shop Automation + Reagent
I have been using
NEBNext kits and am
generally happy with it.
I understand. NEB is a high-performing kit. However, we
have a new compelling alternative that can save you
money, time, and offer flexibility.
Save >$2 prep sample prep (at list price)
Save on sequencing by higher multiplexing
PCR-free workflows
More consistent operator-to-operator reproducibility
Single point of contact for all library prep support
EXTERNAL POSITIONING: NEXTERA®
You Customer
I have been using Nextera
kits and am generally
happy with it.
Nextera® DNA and Nextera® XT DNAand Nextera® DNA Flex Library Preparation Kit NEXTFLEX® Rapid XP DNA-Seq
Kit
$34 or $82 Price Per Sample (no barcodes, only kit) $25
No Beads included? Yes (No additional cost)
No PCR-free workflow compatible? Yes
Locked at 1ng or 50ng only. High risk if badly quantified. (Flex allows 1-500ng) Range of Inputs? Yes
Variable Fragmentation efficiency and library yield Consistent (see data in the deck)
No, locked to tagmentation protocol (~250 or ~350 for Flex) Adjustable fragment size? Yes
>300bp, lose distal 50bp PCR Amplicon compatibility? Yes
Reagent One-stop shop Automation + Reagent
I understand. Illumina would be an obvious choice for library prep.
However, we have a new compelling alternative that can save you
money, time, trouble, and offer flexibility.
Save >$10 on prep sample (at list price)
Adjust fragmentation time and library size for different applications
Range of sample input concentration
PCR-free workflow
More consistent enzymatic fragmentation and high library yield
Single point of contact for all library prep support
NEXTFLEX® RAPID XP DNA-SEQ KIT DIFFERENCE BETWEEN RAPID XP DNA-SEQ AND RAPID DNA-SEQ KIT 2.0
NEXTFLEX® Rapid DNA-Seq 2.0 Kit NEXTFLEX® Rapid XP DNA-Seq Kit
Fragmentation Included? Not included, customer provided (Covaris® etc.) Included (enzymatic)
Turnaround Time ~2 to 3 hours (does not include fragmentation) ~1.5 to 2.5 hours (includes fragmentation)
Input Amount 1 ng to 1 ug 1 ng to 1 ug
# of Cleanup Steps 2 (1 if PCR-free workflow) 2 (1 if PCR-free workflow)
Barcodes Included? NEXTFLEX-HT™ barcodes (bundle) OR bundle
pricing (ex. NEXTFLEX® UDI) Bundle pricing, 50% off NEXTFLEX® barcodes
Beads Included? Yes Yes
Reliable
Convenient
Flexible
Consistent fragmentation, library yields and high-quality
sequencing data
All-inclusive kit that has been streamlined and automated
on Sciclone® and Zephyr® NGS workstations
Use with NEXTFLEX® color-balanced barcodes for
multiplexing up to 384 samples and PCR-free workflows
NEXTFLEX® RAPID XP DNA-SEQ KIT VALUE PROPOSITION
NEXTFLEX® RAPID XP DNA-SEQ KIT TARGET CUSTOMERS
Any DNA-seq application (WGS, WES, targeted seq, cDNA seq, PCR-free seq, etc.)
Tier 1: All Sciclone® and Zephyr® accounts
Tier 2: Core labs with high throughput that may benefit by replacing Covaris® with enzymatic
solution (drivers are performance and price)
Tier 3: Commercial and academic labs performing DNA-seq
Recommended Actions
Send product introduction email/collateral to Sciclone® and Zephyr® accounts
Use promotions to motivate customers to evaluate and switch
NEXTFLEX® RAPID XP DNA-SEQ KIT NEXTFLEX® PROMOS
NEXTFLEX® RAPID XP DNA-SEQ KIT SALES TOOL
Collateral Content Availability Location
Flyer General overview and selling points
October 12
BOX folder
Technical App Note Technical overview and competitor
comparison BOX folder
Internal Product Training Deck General overview and selling points BOX folder
Customer-facing Product Intro Deck General overview and competitive data BOX folder
Website Product page and ordering (SAP) Rapid-XP
User Guide/Manual User guide for library prep.
Instructions to obtain platform scripts BOX folder
Sales Training Product details Week of October 8 Webinar
NEXTFLEX® RAPID XP DNA-SEQ KIT QUESTIONS?
NEXTFLEX® RAPID XP DNA-SEQ KIT APPENDIX
NEXTFLEX® RAPID DNA-SEQ KIT 2.0 (NOT XP) COMPETITOR DETAILS
Kapa® HyperPrep™
Kit
Illumina® Nextera® or Nextera® XT DNA
Library Preparation Kit
Illumina® Nextera® DNA Flex Library Preparation Kit
Illumina® Truseq® Nano DNA Kits or
PCT-free Kits
NEBNext® Ultra™ II DNA Library Prep With Sample
Purification Beads
NEXTFLEX® Rapid DNA-Seq 2.0 kit
NEXTFLEX® Rapid DNA-Seq 2.0 Bundle
kit
Kit Price
$2,469 (96rxn), $720 (24rxn), $272
(8rxn)
$7870 (96rxn), $2200 (24rxn) OR $3270
(96rxn), $860 (24 rxn)
$4032 (96rxn), $1008 (24rxn)
$2650 (96rxn), $660 (24rxn)
$2465 (96rxn), $645 (24rxn) $2208 (96rxn), $1132 (48rxn), $194 (8rxn)
$2640 (96rxn), $1353 (48rxn), $232 (8rxn)
Price Per Sample (including barcode
purchase) $39 - $31
$85 - $94 OR $37 - $39
$47 $33 - $35 $30 - $45 $23 - $24 $27.5 - $29
Package Size (RXNs) 8, 24, 96 24, 96 24, 96 24, 96 24, 96 8, 48, 96 8, 48, 96
Fragmentation included? No Yes Yes No No No No
Beads included? No No No Yes Yes Yes Yes
Barcodes included? No No No No No No Yes (8, 48, 96 –HT
barcodes)
How many barcodes available?
24 SI or 96 DI 96 96 24 SI or 96 DI 12 SI or 96 DI 384 NA
Minimum input amount 1 ng 50 ng or 1ng 1 ng (rec 100ng) 100ng or 1ug 500 pg 1ng 1ng
Turnaround time ~2-3 hours ~1.5 hours ~1.5 hours ~5-6 hours ~2.5 hours ~3 hours ~3 hours
Yield +++ unknown unknown unknown ++++ ++++ ++++
*Pricing information and attributes found on vendor’s websites on 6-7-18
NEXTFLEX® RAPID XP DNA-SEQ KIT COMPETITOR DETAILS *Pricing information and attributes found on vendor’s websites on 10-6-18
Nextera® DNA Library
Preparation Kit
Nextera® XT DNA
Library Preparation Kit
Nextera® DNA Flex
Library Prepparation Kit
Kapa® HyperPlus™
Library Prep Kit w amp
NEBNext® Ultra™ II FS DNA
Library Prep for Illumina®
Qiagen® QIAseq® FX
DNA Library Kit
Swift® S2 Turbo™/Turbo
Flexible™ DNA Library Kits
NEXTFLEX® Rapid
XP DNA-Seq Kit
Kit Price (large size) $7,870 $3,270 $4,032 $3,072 $2,400 $3,280 $1,440 $1,200
Price Per Sample (no
barcodes, just kit) $82 $34 $42 $32 $25
$34 (includes
barcodes) $15 $25
Large Size (RXNs) 96 96 96 96 96 96 96 48
Fragmentation included? Yes Yes Yes Yes Yes Yes Yes Yes
Beads included? No No No for size selection No No No No Yes
Barcodes included? No No No No No Yes No No, price bundling
How many barcodes
available? 96 384 96 96 96 96 96 384
Minimum input amount 50 ng lock 1 ng lock
1ng (microbial), 100 ng
(complex and for
integrated normalization)
1 ng (microbial), 50 ng
(complex) 500 pg 1ng 1ng 1 ng
PCR-free workflow
compatible? No No No Yes No Yes No Yes
Turnaround time ~1.5 hours ~1.5 hours
~ 3-4 hours (INCLUDING
DNA extraction and
library normalization)
~1.5 to 2.5 hours ~2.5 hours ~2.5 hours ~1.5 to 2.5 hours ~1.5 – 2.5 hours
Notes:
Free-Adapter Blockers
(+ $).
Input is locked.
Not PCR-free
compatible.
For small genomes.
Free-Adapter Blockers
(+ $)
Input is 1ng only.
Not PCR-free
compatible.
Small, large, human
genomes
Blood, gDNA, Saliva
Bead-linked
transposome can do
tagmentation, index
tagging, normalization in
one step
Flex Lysis (for blood,
0.5hrs added only)
Market Leader.
Fragmentation and end-
repair/adenylation are
separate steps.
Quality brand.
Not PCR-free compatible,
barcoded primers contribute
significantly to index hopping
on patterned flow cells.
Includes barcode plate
with pierceable seal
for partial plate usage.
Up to 768-plex using
Combinatorial DI
(complicated)
Not PCR-free compatible,
barcoded primers contribute
significantly to index
hopping on patterned flow
cells.
Very EDTA sensitive
Sell together with
superior quality of our
barcodes.
Yield No notes/++ No notes/++ No notes/++ +++ ++++ No notes on yield No notes on yield +++
Kapa®’s Inconsistent Fragmentation
*from NEB® technical document available online 10-4-18. *from Agilent® technical document available online 10-4-18.
NEB®’s Variation by Operator
*from NEB® technical document available online 10-4-18. Same Control sample produced variable results for each User.
NEXTERA®’s Locked Tagmentation Protocols
*from NEB® technical document available online 10-4-18.
*from Illumina® technical document available online 10-5-18.