201912v51
Newsletter
NMDA 38
Ru Fc 40



59
2



1970 80

3


4







2014 72016 10 JST



2 PPI
PPI Fig. 1a 1[5-8]

Newsletter-- Vol.34, No.2 (2019. 12)
5




[9] 2. COI1-JAZ in vitro in silico JA-Ile COI1-JAZ
COR, 3
COR JAZ
[10,11]COR
CFA, 4
CMA, 5
4 3, ent3, 6, ent6, Fig. 2 COI1-JAZ9
Y2H4

[11] ent6 Y2H COI1-JAZ9
JAZ COI1-JAZ
Y2H Y2H
COI1-JAZ

JAZ
JAZ

Figure 1. (a-c) a: [2]b:
[3]c: [5]. (d) 1 JA-Ile2
COI1-JAZ .
6
COI1-COR- JAZ1 X [5]COI1
JAZ1 COI1-COR Jas motif

JAZ Jas motif
anti-Fluorescein JAZ
JAZ1 GST COI1 COR3 COR
ent3anti-Fluorescein anti-GST
Fig. 2aCOI1-JAZ1 COR JA-Ile PPI ent3 JA
COI1-JAZ PPI Fig. 2b 4 COR Jas motif
JAZ COI1 PPI COR COI1-JAZ
ent6 COI1-JAZ3, 9-12 5 PPI ent6 in vitro
JAZ
COR-JAZ1 JAZ in silico docking study
COR
ent6 COI1-JAZ3, 9-12 COR
Figure 2. (a) OG JAZ pull-down . (b)
. (c) COR . (d) COR COI1-JAZs
(I.S. = ).
7

NOMeNOPh NOBn
JAZ
NOPh8 JAZ9 10
13 JAZ 2
Fig. 3b 3. COI1-JAZ in vivo 8
1 COR
ent6 8

8
PDF1.2 COR 5
ORA59
50 µM 5 A. brassicola COR


Fig. 4[9] in vitro JAZ9/10
in vivo
JAZ
ORA59 jaz10-1 mutant
jaz9-1 mutant

Figure 3. (a) ent6. (b) ent6, 79 COI1/JAZ .
Figure 4. COR (3) NOPh (8)
(a) (b).
8
[13] 8 COI1/JAZ9
ERF1-EIN3/EIL1-ORA59 MYC
COR JA-Ile
in vitro JAZ

JAZ 4.
2016 Greg Howe 13 JAZ 5 5
6
[14] 10 11 JAZ jazD, jazU[15] JAZ


[17] ITbM
Bump-and-hole [18]


2018 in silico
Roberto Solano Andrea Chini
JST JSPS
[1] Jiang, K.; Asami, T. Biosci. Biotechnol. Biochem., 2018, 82, 1265. [2] Murase, K.; Hirano, Y.; Sun, T.-P.; Hakoshima,
T., Nature 2018, 456, 459 (PDB ID: 2ZSH). [3] Tan, X. et al. Nature 2007, 446, 640 (PDB ID: 2PIQ). [4] Santiago, J. et
al. Nature 2009, 462, 665. [5] Sheard, L. B. et al. Nature 2010, 468, 400 (PDB ID: 3OGM). [6] Thins, B. et al. Nature 2007,
448, 661. [7] Chini, A. et al. Nature 2007, 448, 666. [8] Fonseca, S. et al. Nat. Chem. Biol., 2009, 5, 344. [9] Takaoka, Y. et al.
Nat. Commun. 2018, 9, 3654. [10] Okada, M. et al. Org. Biomol. Chem. 2009, 7, 3065. [11] Ueda, M. et al. ACS Cent. Sci.
2017, 3, 462. [12] Song, S. et al. Plant Cell 2014, 26, 263. [13] Zhu, Z. et al. Proc. Natl. Acad. Sci. USA 2011, 108, 12539.
[14] Campos, M. L. et al. Nat. Commun. 2016, 7, 12570. [15] Guo, Q. et al. Proc. Natl. Acad. Sci. USA 2018, 115, E10768.
[16] Halder, V.; Russinova, E. Nat. Chem. Biol. 2019, 15, 1025. [17] Vaidya, A. S. et al. Science 2019, 366, 446. [18] Uchida,
N. et al. Nat. Chem. Biol. 2018, 14, 299.
Newsletter-- Vol.34, No.2 (2019. 12)
9

2011–2012
2012–2019
2016–2018 Nathan W. Luedtke
2019 7





BrdU DNA
Fig. 11BrdU 5


10
5-ethynyldeoxyuridineEdU
DNA 2EdU Huisgen Copper catalyzed Azide-Alkyne CycloadditionCuAACDNA
CuAAC
3 DNA
4CuAAC
DNA 5-azidomethyldeoxyuridineAmdU
dimorpholino- cycloocadiyneDiMOC DNA

2. POM-AmdUChemBioChem, 2018, 19, 1939. Luedtke 5
AmdU 5AmdU Strain-Promoted Azide-Alkyne CycloadditionSPAAC 6
AmdU
NH2 O
PO OPh
NH O
OAB I2, pyridine
OAB O
11
HINT-1 ProtideProt10
Scheme 1AmdU DNA
3
HeLa 24 DNA
CuAAC DNA Fig. 2
POM-AmdU AB-AmdU DNA AmdUProt- AmdU HeLa AmdU
HINT-1 AB-AmdU HeLa
U2OS 12
AB-AmdU POM-AmdU AB-AmdU 2
AB-AmdU
11POM-AmdU DNA
POM-AmdU U2OS AML
POM-AmdU

Fig. 2 AmdU POM-, AB-, Prot-AmdU 10 µM HeLa
24 . 2M HCl DNA AlexaFluoro-594-
Huisgen DNA . POM-AmdU
DAPI.
12

DNA

2 122010 CODY
SPAAC 13

Scheme 2 8
14 DiMOC
DiMOC X
CODY
DiMOC
> 10 mM DiMOC DNA DiMOC DNA
DNA DiMOC UV
DiMOC DNA 3 1
15 µM DiMOC AmdU
1.0 M-1•sec-1 DNA
DiMOC DNA

BCN-TAMRA 50 4%
AmdU BCN
Scheme 2. Dimorpholino cyclooctadiyne (DiMOC) . NBS = N-bromosuccinimide, AIBN = azobis(isobutyronitrile), DIBAL-H = diisobutylaluminium hydride, LHMDS = lithium bis(trimethylsilyl)amide, LDA = lithium diisopropylamide.
CO2H
Me
LHMDS, ClP(O)(OEt)2 -78ºC
ON NO
13
ODN-1 DiMOC N3- TAMRA ODN-1 5

DNA
DAPI DiMOC
AmdU


2'(R)-azidodeoxyadenosineDNA
Fig. 4 RNA 2'(R)-azidodeoxy adenosineDNA AzC
.
Fig. 3 a) DiMOC AmdU DNA SPAAC . b) BCN-TAMRA N3-TAMRA
. c) AmdU DNAODN-1 BCN-TAMRA DiMOC/N3-TAMRA
. ODN-1 T AmdU . d) POM- AmdU HeLa BCN-TAMRA DiMOC/N3- TAMRA DNA .
Newsletter-- Vol.34, No.2 (2019. 12)
14
DiMOC N3-TAMRA RNADNA
AzC DiMOC/N3-TAMRA DNA AzC
15

3-2. DiMOC DNA Angew. Chem. Int. Ed., 2018, 57, 15405. Cis-Pt DNA-DNA ICL

POM-AmdU DNA 2
DiMOC DNA-DNA
AmdU DiMOC ICL
Fig. 5 ODN-2 AmdU ODN-3
AmdU Fig. 5a
ODN DiMOC 2 ODN-2
Fig 5b, lane 2 MALDI-MS ICL Fig. 5c 2.1 x 105 M-1•sec-1
AmdU DiMOC 10 DiMOC
DNA HeLa POM-AmdU 72
2 PD time = 30 DiMOC
ICL
denaturing-renaturing ICL POM-AmdUDiMOC
Fig. 5 a) DiMOC AmdU DNA ICL . b) ODN-2ODN-3 DIMOC
. c) Lane 2 MALDI-MS .
Newsletter-- Vol.34, No.2 (2019. 12)
15
DNA 100-300 bp DNA

ICL
Fig. 6aICL
POM-AmdU DiMOC DNA S1
Fig. 6b, lane 8, 10, 12
UPLC-MS ICL
Fig. 6c, dAmdU-DiMOC ICL
DiMOC POM- AmdU DiMOC

DiMOC SPAAC
DiMOC

Fig. 6 a) DNA ICL denaturing-renaturing . b) DNA
denaturing-renaturing S1 . c) S1

UHPLC-MS m/z=513.20–513.21 SIM . d) ICL .
Newsletter-- Vol.34, No.2 (2019. 12)
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POM-AmdU DiMOC DNA
POM-AmdU HeLa IC50 > 100 µMDiMOC DNA BrdU EdU
DiMOC
Department of Chemistry, University of Zurich Nathan W. Luedtke McGill UniversityLuedtke DiMOC X
Tony Linden University of ZurichUPLC-MS Jorn Piel
ETH Zurich
Dr. Helmut Legerlotz Foundation [1] R. C. Leif, J. H. Stein, R. M. Zucker, Cytometry 2004, 58A, 45–52. [2] A. Salic, T. J. Mitchison, Proc. Natl. Acad. Sci. 2008, 105, 2415–2420. [3] D. C. Kennedy, C. S. McKay, M. C. B. Legault, D. C. Danielson, J. A. Blake, A. F. Pegoraro, A. Stolow, Z.
Mester, J. P. Pezacki, J. Am. Chem. Soc. 2011, 133, 17993–18001. [4] S. H. Chiou, J. Biochem. 1983, 94, 1259–1267. [5] A. B. Neef, N. W. Luedtke, ChemBioChem 2014, 15, 789–793. [6] N. J. Agard, J. A. Prescher, C. R. Bertozzi, J. Am. Chem. Soc. 2004, 126, 15046–15047. [7] M. E. Black, L. A. Loeb, Biochemistry 1993, 32, 11618–11626. [8] D. Farquhar, D. N. Srivastva, N. J. Kuttesch, P. P. Saunders, J. Pharm. Sci. 1983, 72, 324–325. [9] W. Thomson, D. Nicholls, W. J. Irwin, J. S. Al-Mushadani, S. Freeman, A. Karpas, J. Petrik, N. Mahmood, A.
J. Hay, J. Chem. Soc. Perkin Trans. 1 1993, 1239–1245. [10] C. McGuigan, R. N. Pathirana, N. Mahmood, K. G. Devine, A. J. Hay, Antiviral Res. 1992, 17, 311–321. [11] J. L. Bolton, Curr. Org. Chem. 2014, 18, 61–69. [12] H. N. C. Wong, P. J. Garratt, F. Sondheimer, J. Am. Chem. Soc. 1974, 96, 5604–5605. [13] I. Kii, A. Shiraishi, T. Hiramatsu, T. Matsushita, H. Uekusa, S. Yoshida, M. Yamamoto, A. Kudo, M.
Hagiwara, T. Hosoya, Org. Biomol. Chem. 2010, 8, 4051. [14] F. Xu, L. Peng, K. Shinohara, T. Morita, S. Yoshida, T. Hosoya, A. Orita, J. Otera, J. Org. Chem. 2014, 79,
11592–11608. [15] T. Triemer, A. Messikommer, S. M. K. Glasauer, J. Alzeer, M. H. Paulisch, N. W. Luedtke, Proc. Natl. Acad.
Sci. 2018, 115, E1366–E1373.
Newsletter-- Vol.34, No.2 (2019. 12)
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DNA RNA

GNA[4]
XNA
D-aTNA (acyclic D- Threoninol Nucleic Acid)[5], L-aTNA (acyclic L-
Figure 1.
18
Threoninol Nucleic Acid)[6], SNA (Serinol Nucleic Acid)[7] 3
(Fig. 1) D-aTNA DNA RNASNA
L-aTNA

[8]
XNA XNA

XNA

DNA (Fig. 3)[10]
DNA L-aTNA N-Cyanoimidazole


2. L-aTNA 16-mer L-aTNA (Template) 8-mer L-aTNA (8A8B)8B
3’8A 1’8A 3’
PAGE (Fig. 4)
Figure 2.
19
DNA ( 27%) L-aTNA (74%)
N-Cyanoimidazole
L-aTNA DNA
DNA

N-Cyanoimidazole

DNA 2
DNA 1
L-aTNA
2
Figure 4. L-aTNA () () Reaction conditions: 4oC, 12 h, [oligomer] = 1.0 μM, 20 mM N-Cyanoimidazole, 20 mM ZnCl2, 100 mM NaCl, Denaturing PAGE: 15% AA, 8 M Urea, 1.5 h, 750 V.
Figure 5.
Reaction conditions: 25oC, [oligomer] = 1.0 μM, 10 mM N- Cyanoimidazole, 20 mM MnCl2, 100 mM NaCl.
Newsletter-- Vol.34, No.2 (2019. 12)
20
L-aTNA

L-aTNA (Fig. 6)
PCR
L-aTNA
8- mer 5-mer, 4-mer, 3-mer, 2- mer

(Fig. 7)6h 5- mer 4-mer
24h 3-mer
DNA
Figure 7. L-aTNA
Reaction conditions: 4oC, [8A] = [Template] = 1.0 μM, [5B] = [4B] = [3B] = [2B] = 10 μM, 10 mM N-Cyanoimidazole, 20 mM MnCl2, 100 mM NaCl.
Figure 6. L-aTNA
Newsletter-- Vol.34, No.2 (2019. 12)
21
(Fig. 8) 12-mer
16-mer
12h 84% A
L- aTNA

L-aTNA DNA

L-aTNA SELEX XNA
XNA Prebiotic world ”XNA world”

Figure 8.
Reaction conditions: 4oC, [8A] = [Template] = 1.0 μM, [4B] = [4B’] = 10 μM, 10 mM N-Cyanoimidazole, 20 mM MnCl2, 100 mM NaCl.
Newsletter-- Vol.34, No.2 (2019. 12)
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[1] (a) Obika, S.; Nanbu, D.; Hari, Y.; Andoh, J.; Morio, K.; Doi, T.; Imanishi, T. Tetrahedron Lett. 1998, 39, 5401.;
(b) Singh, S. K.; Nielsen, P.; Koshkin, A. A.; Wengel, J. Chem. Commun. 1998, 455 [2] Campbell, M. A.; Wengel, J. Chem. Soc. Rev. 2011, 40, 5680. [3] Schneider, K. C.; Benner, S. A. J. Am. Chem. Soc. 1990, 112, 453. [4] Zhang, L. L.; Peritz, A.; Meggers, E. J. Am. Chem. Soc. 2005, 127, 4174. [5] Asanuma, H.; Toda, T.; Murayama, K.; Liang, X.; Kashida, H. J. Am. Chem. Soc. 2010, 132, 14702. [6] Murayama, K.; Kashida, H.; Asanuma, H. Chem. Commun. 2015, 51, 6500. [7] Kashida, H.; Murayama, K.; Toda, T.; Asanuma, H. Angew. Chem., Int. Ed. 2011, 50, 1285. [8] Pinheiro, V. B.; Taylor, A. I.; Cozens, C.; Abramov, M.; Renders, M.; Zhang, S.; Chaput, J. C.; Wengel, J.;
Peak-Chew, S-Y.; McLaughlin, S. H.; Herdewijn, P.; Holliger, P. Science 2012, 336, 341. [9] Silverman, A. P.; Kool E. T. Chem. Rev. 2006, 106, 3775. [10] Kanaya, E.; Yanagawa, H. Biochemistry 1986, 25, 7423.
Newsletter-- Vol.34, No.2 (2019. 12)
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PPI PPI


1990


NSA 1a[4]
φ, ψ, ωφψ 1,3-
bω
Newsletter-- Vol.34, No.2 (2019. 12)
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N N

2006 Arvidsson [6] N

NSA
NSA MD
NSA 500
0.8
1.6
NSA

NSA DEER
NSA
NSA

NSA (a)
(b) NSA φψ(c) NSA

25
1HCOSYHMQCHMBC NMR
NOESY

4. NSA PPI NSA
NSA N

PPI MDM2 p53
MDM2 p53
p53 C transactivation domainTAD
p53-TAD Phe19Trp23Leu26 a[7]

NSA N p53-TAD
b1 N
NSA
c
NSA KD = 1.1 µM MDM2

NSA MDM2 p53-TAD
Ki = 0.93 µM MDM2 p53-TAD
NSA
MDM2 NSG
DEER NSA NSG
Tikhonov
26
NSG MDM2 NSA MDM2
PPI
PPI
PPI [8] Kodadek Lim
[9,10]


D2
MDM2-p53 NSA PPI (a) p53-TAD MDM2 (b)
p53-TAD NSA p53 F19W23
L26 αβ NSA Nα (c) p53
NSA MDM2-p53

27


CREST [1] Simon, R. J.; Kania, R. S.; Zuckermann, R. N.; Huebner, V. D.; Jewell, D. A.; Banville, S.; Ng, S.; Wang, L.;
Rosenberg, S.; Marlowe, C. K.; Spellmeyer, D. C.; Tan, R.; Frankel, A. D.; Santi, D. V.; Cohen, F. E.; Bartlett, P. A. Proc. Natl. Acad. Sci. U. S. A. 1992, 89, 9367−9371.
[2] Yu, P.; Liu, B.; Kodadek, T. Nat. Biotechnol. 2005, 23, 746−751. [3] Kodadek, T.; McEnaney, P. J. Chem. Commun. 2016, 52, 6038–6059. [4] Gao, Y.; Kodadek, T. Chem. Biol. 2013, 20, 360–369. [5] Morimoto, J.; Fukuda,Y.; Kuroda, D.; Watanabe, T.; Yoshida, F.; Asada, M.; Nakamura, T.; Senoo, A.;
Nagatoishi, S.; Tsumoto, K.; Sando, S. J. Am. Chem. Soc., 2019, 141, 14612−14623. [6] Zhang, S.; Prabpai, S.; Kongsaereeb, P.; Arvidsson, P. I. Chem. Commun. 2006, 497–499. [7] Kussie, P. H.; Gorina, S.; Marechal, V.; Elenbaas, B.; Moreau, J.; Levine, A. J.; Pavletich, N. P. Science 1996,
274, 948−953. [8] Shneider, J. A.; Craven, T. W.; Kasper, A. C.; Yun, C.; Haugbro, Briggs, E. M.; Svetlov, V.; Nudler, E.; Knaut,
H.; Bonneau, R.; Carabedian, M. J.; Kirshenbaum, K. Logan, S. K. Nat. Commun. 2018, 9, 4396. [9] Trader, D. J.; Simanski, S.; Kodadek, T. J. Am. Chem. Soc. 2015, 137, 6312–6319. [10] Oh, M.; Lee. J. H.; Moon, H.; Hyun, Y. J.; Lim, H. S. Angew. Chem. Int. Ed. 2016, 55, 602–606.
Newsletter-- Vol.34, No.2 (2019. 12)
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P-
-
2
PPI
P-
S-S
20
29
2P-
()Y140 (
)()

X
X X
(Fig. 3) PPI
PPI
P-


JSPS [1] Kudo, S.; Caaveiro, M. M. J.; Tsumoto, K. Structure, 2016, 24, 1523. [2] Senoo, A.; Nagatoishi, S.; Moberg, A.; Babol, N. L.; Mitani, T.; Tashima, T.; Kudo, S.; Tsumoto, K. Chem. Commun., 2018, 54, 5350. [3] Kudo, S.; Caaveiro, M. M. J.; Nagatoishi, S.; Miyafusa, T.; Matsuura T.; Sudou Y.; Tsumoto K. Sci. Rep., 2017, 7, 39518
Newsletter-- Vol.34, No.2 (2019. 12)
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“
31

SELEX FGFR2
DNA
FGFR2 A
FGFR2
FGFR2 2.




[1] Sarabipour and Hristoba. Nat. Commun. 2016, 4:7, 10262. [2] Ueki et al., Angew. Chem. Int. Ed., 2016, 55, 579. [3] Ueki et al., J. Am. Chem. Soc. 2017, 139, 6554.
. Aptamer
32
photoSLIPT
1 2JST
1990



1.

CFPGFPYFP mCherry

SLIPT


a
b
33
eDHFR TMP
o-(NVOC)
NVOC CFPGFP

(PIP3)PIP3
PIP3
PI3K mDcTMPNVOC
( 2a)PI3K p110 iSH2 eDHFR
(eDHFR-Venus-iSH2)eDHFR-Venus-iSH2 iSH2
eDHFR-Venus-iSH2 mDcTMPNVOC
3
( 2b)photoSLIPT


[1] Ishida, M.; Watanabe, H.; Takigawa, K.; Kurishita, Y.; Oki, C.; Nakamura, A.; Hamachi, I.; Tsukiji, S. J. Am.
Chem. Soc. 2013, 135, 12684. [2] Nakamura, A.; Katahira, R.; Sawada, S.; Shinoda, E.; Kuwata, K.; Yoshii, T.; Tsukiji, S. Biochemistry. in press [3] Nakamura, A.; Oki, C.; Kato, K.; Fujinuma, S.; Maryu, G.; Kuwata, K.; Yoshii, T.; Matsuda, M.; Aoki, K.;
Tsukiji, S. ChemRxiv, DOI: 10.26434/chemrxiv.8222456
a
b
34
RNA
4



RNA G- quadruplex 1)G-quadruplex

G-quadruplex
RT 3)RNA
cDNA
G-quadruplex Fig. 2
RNA Staple DNA
Fig. 1. Staple mRNA RNA G-quadruplex
Newsletter-- Vol.34, No.2 (2019. 12)
35
RNA G-quadruplex
TPM3 5’UTR
Firefly luciferaseFluc mRNA
Staple
Staple G-quadruplex
TPM3 Staple



4. Staple mRNA G-quadruplex


Staple Staple
RNAi


[1] Kumari, S.; Bugaut, A.; Huppert, J. L.; Balasubramanian, S. Nat. Chem. Biol. 2007, 3, 218–221 [2] Xu, S.; Li, Q.; Xiang, J.; Yang, Q.; Sun, H.; Guan, A.; Wang, L.; Liu, Y.; Yu, L.; Shi, Y.; Chen, H.; Tang, Y. Sci.
Rep. 2016, 6, 24793. [3] Hagihara, M.; Yoneda, K.; Yabuuchi, H.; Okuno, Y.; Nakatani, K. Bioorg. Med. Chem. Lett., 2010, 20, 2350-
2353.
Fig. 2. RT
36
1
1 1 2 3 1 4 4 5 6 7 3,
8 1, 9, 10
123456
789 10AMED-CREST


37
[2]

alkaline
phosphatases (ALPs)
ALPTNAP
Fig.3
ectonucleotide
pyrophosphatases/phosphodiesterases (ENPPs)

(Fig 5)
[1] R.Watanabe et al., Nat. Commun, 2014, 5, 4519. [2] S.Sakamoto et al., Sci. Adv, in press
Fig 4. AENPP
B

A
B

38
NMDA
………

(NMDAR)AMPA (AMPAR)
AMPAR



NMDAR NMDAR
NMDAR
39
NMDA




[1] Fujishima, S. et al. J. Am. Chem. Soc., 2012, 134, 3961. [2] Wakayama, S. et al. Nat. Commun., 2017, 8,14850
2(a)
(b) 2
LDAI (0h)
LDAI()
40
1 2
1,2, 1,2, 1, 1
2011 -2016
2016 -2018
2018 -2020


[1] ADC
Fc

1-methyl-4-aryl-urazole(MAUra) Ru
Fc
Figure 1.
41
ApA[4] Ru [5]
Fc (Figure 2a)Ru
HER2 CD20
EGFR Fc (Figure 2b)
LC-MS/MS Fc
Ru


[1] Beck, A.; Goetsch, L.; Dumontet, C.; Corvaïa, N. Nat. Publ. Gr. 2017, 16 (5), 315.
[2] Yamada, K.; Ito, Y. ChemBioChem 2019, 20, 2729.
[3] Sato, S.; Hatano, K.; Tsushima, M.; Nakamura, H. Chem. Commun. 2018, 54 (46), 5871.
[4] Li, R.; Dowd, V.; Stewart, D. J.; Burton, S. J.; Lowe, C. R. Nat. Biotechnol. 1998, 16, 190.
[5] Tsushima, M.; Sato, S.; Niwa, T.; Taguchi, H.; Nakamura, H. Chem. Commun. 2019, 55, 13275.
Figure 2. Fc
42


1,2,3
,4,5JST-PRESTO,6AMED-CREST
4, 1,2,6



1
g-GGT

43
2 X

X

X Y
Y
GGT
Y GGT




[1] Urano, Y. et al. Science Transl Med, 3, 110 (2011). [2] Ueo, H. et al. Sci. Rep., 5, 12080 (2015). [3] Asanuma,
D. et al. Nat. Commun., 6, 6463 (2015). [4] Komatsu, T. et al. J. Am. Chem. Soc., 135, 6002-6005 (2013).
Newsletter-- Vol.34, No.2 (2019. 12)
44




(Q)

45

D-aTNA
aTNA
Dabcyl

Q1
Q3 Cy3
FAM
9
(Fig.5)
[1] H. Asanuma, et al., J. Am. Chem. Soc., 2010, 132, 14702-14703. [2] K. Murayama, et al., ChemBioChem, 2015, 16, 1298-1301.
Fig. 2. DNA D-aTNA
Fig. 3
46




WPI






47



Newsletter-- Vol.34, No.2 (2019. 12)
48


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Newsletter-- Vol.34, No.2 (2019. 12)
54
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230
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MPC



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56
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Newsletter-- Vol.34, No.2 (2019. 12)
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53


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*1 Molecular Omics *2 Organic & Biomolecular Chemistry *3 Metallomics


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