f h i ll i iliNews from the Live Cell Imaging Facility

Pi C l PhDPina Colarusso, PhDScientific Director

J 9 2011June 9, 2011

LCI ToolkitImage processing

Wide‐field  (white‐light,  fluorescence, deconvolution)

Image processing and analysis

Spinning disk confocal

Laser scanning confocalConsultations

Training

Workshops and seminarsMultiphotonMultiphoton

Demonstrations

STEDDarkfield

from the New York Times

MICROBES CAUGHT IN ACTIONMoving Pictures of Them a Great Aid In Medical Research.

Alisa W. Serio, Robert L. Jeng, Cat M. Haglund, Shawna C. Reed and Matthew D. Welch Cell Host & Microbe, Volume 7, Issue 5, 20 May 2010, Pages 388‐398

Support Equipment AvailableSupport Equipment Available

Temperature, humidity, CO2, flow

Spotlight on Multiphoton

11‐‐photon prop. Iphoton prop. I22‐‐photon prop. I photon prop. I 22

Principles of Two‐Photon ExcitationPrinciples of Two Photon Excitation

Depth Resolution: Confocal vs. l i hMultiphoton

Rat optic nerve loaded with the Ca‐sensitive dye X‐rhod‐1 (red) and fluorescein dextran (green).

Courtesy of Ileana Micu, PhDHotchkiss Brain Institute

Multiphoton vs. ConfocalMultiphoton vs. Confocal

• > λ used in multiphoton

• Damage• Depth• Expense• Expense• Characterization

Courtesy of Laura Milne and Dr. Bjoern Petri, Kubes Group

Courtesy of Dr. Bjoern Petri, Phenomics Core, Snyder Institute

Upcoming : Stimulated Depletion Emission Depletion

Garth J. Simpson, Nature 440, 879‐880 (13 April 2006) 

Importance of TeamsImportance of Teams

Craig Brideau, Electrical enigineerPeter Stys MD Professor HBIPeter Stys, MD, Professor, HBI

Stimulated Depletion Emission Depletion

http://www.leica‐microsystems.com/

Reorganization of VE-cadherin under flow with nanoscaleresolution STED-microscopy.

Seebach J et al. Cardiovasc Res 2007;75:598-607

Copyright © 2007, European Society of Cardiology

www.iii.ucalgary.ca/imaging

LCI StaffLCI Staff

Kasia Stevens, PhDI Vi i i i li

Jen AmonO i l I i S i liIn Vivo imaging specialist Optical Imaging Specialist

Last but not least!Last but not least!

Faculty Directorsy

Donna‐Marie McCafferty, PhDKamala Patel, PhD