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New HABs monitoring tools amp approaches for developing and
maintaining collaborative networksUSEPA Region 10 HABs workshop
Alan Wilson
Fisheries - Auburn University
29-20 March 2016
Aquaculture pondAlabama August 2008
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Lake Fox16 January 2016
03 m Secchi depth
What is a bloom
Auburn Univ pond7 August 2009
What is a bloom
Fayette County Fishing Lake10 August 2009024 m Secchi depth118 μgL chlorophyll
What is a bloom
bull World Health Organization
bull httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Chorus and Bartram 1999
WHO ThresholdsCell density ge 20000 cellsmlChlorophyll ge 10 microgLMicrocystin drinking water ge1 microgL
What is a bloom
bull World Health Organization
bull US Environmental Protection Agency
bull httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
USEPA drinking water advisoriesmicrocystin
03 microgL children 0-6yrs16 microgL gt6yrs
cylindrospermopsin07 microgL children 0-6yrs30 microgL gt6yrs
What is a bloom
bull World Health Organization
bull US Environmental Protection Agency
bull Carlson Trophic State Index (TSI)bull widely used classification scheme
bull based on transparency (Secchi depth) chlorophyll a total phosphorus to relate to algal biomass typically during the summer
bull scale from 0 ndash 110 increase of 10 TSI units = 2x algal biomassbull TSI useful for comparing lakes within a region and for assessing
changes in trophic status over timebull httpaslonetlotocvol_22issue_20361pdf
What is a bloom
TSI values vs water quality
lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year
40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery
50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo
60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses
gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses
Carlson 1977 Limnology and Oceanography
TSI values
Oligotrophic Mesotrophic Eutrophic Hypereutrophic
Trophic state index
Secchi depth(m)
Chlorophyll(microgL)
Total Phosphorus
(microgL)
NOT A BLOOM
BLOOM
Carlson 1977 Limnology and Oceanography
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
What is a bloom
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Lakesidemonitoringapproaches
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Lake Fox16 January 2016
03 m Secchi depth
bull Important targetsbull Toxigenic cyanobacteria
bull Toxins (cell-bound andor dissolved)
bull Poor transparency
MicrocystisChroococcus Planktothrix
Cylindrospermopsis
Anabaena
Merismopedia Lyngbya Coelosphaerium
How do we quantify cyanobacterial abundance
Huge size range
Kalff 2002 Limnology
Algal group Length (microm)
PicoplanktonNanoplanktonMicroplanktonMesoplankton
02-2020-3030-200
200-20000
Burj Khalifa in Dubai (830m)
Picoplankton Mesoplankton
100000xdifference
Human (2m)
Stacked 240x to reach 200000m
Common techniques for enumerating phytoplankton
Hydrobios settling chambers
High performanceliquid chromatography
Flow CAM imaging particle analyzer
Quantitative PCR
Benchtop fluorometerTurner Designs Trilogy
Field sondeHydrolab minisonde
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Lake Fox16 January 2016
03 m Secchi depth
What is a bloom
Auburn Univ pond7 August 2009
What is a bloom
Fayette County Fishing Lake10 August 2009024 m Secchi depth118 μgL chlorophyll
What is a bloom
bull World Health Organization
bull httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Chorus and Bartram 1999
WHO ThresholdsCell density ge 20000 cellsmlChlorophyll ge 10 microgLMicrocystin drinking water ge1 microgL
What is a bloom
bull World Health Organization
bull US Environmental Protection Agency
bull httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
USEPA drinking water advisoriesmicrocystin
03 microgL children 0-6yrs16 microgL gt6yrs
cylindrospermopsin07 microgL children 0-6yrs30 microgL gt6yrs
What is a bloom
bull World Health Organization
bull US Environmental Protection Agency
bull Carlson Trophic State Index (TSI)bull widely used classification scheme
bull based on transparency (Secchi depth) chlorophyll a total phosphorus to relate to algal biomass typically during the summer
bull scale from 0 ndash 110 increase of 10 TSI units = 2x algal biomassbull TSI useful for comparing lakes within a region and for assessing
changes in trophic status over timebull httpaslonetlotocvol_22issue_20361pdf
What is a bloom
TSI values vs water quality
lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year
40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery
50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo
60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses
gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses
Carlson 1977 Limnology and Oceanography
TSI values
Oligotrophic Mesotrophic Eutrophic Hypereutrophic
Trophic state index
Secchi depth(m)
Chlorophyll(microgL)
Total Phosphorus
(microgL)
NOT A BLOOM
BLOOM
Carlson 1977 Limnology and Oceanography
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
What is a bloom
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Lakesidemonitoringapproaches
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Lake Fox16 January 2016
03 m Secchi depth
bull Important targetsbull Toxigenic cyanobacteria
bull Toxins (cell-bound andor dissolved)
bull Poor transparency
MicrocystisChroococcus Planktothrix
Cylindrospermopsis
Anabaena
Merismopedia Lyngbya Coelosphaerium
How do we quantify cyanobacterial abundance
Huge size range
Kalff 2002 Limnology
Algal group Length (microm)
PicoplanktonNanoplanktonMicroplanktonMesoplankton
02-2020-3030-200
200-20000
Burj Khalifa in Dubai (830m)
Picoplankton Mesoplankton
100000xdifference
Human (2m)
Stacked 240x to reach 200000m
Common techniques for enumerating phytoplankton
Hydrobios settling chambers
High performanceliquid chromatography
Flow CAM imaging particle analyzer
Quantitative PCR
Benchtop fluorometerTurner Designs Trilogy
Field sondeHydrolab minisonde
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Lake Fox16 January 2016
03 m Secchi depth
What is a bloom
Auburn Univ pond7 August 2009
What is a bloom
Fayette County Fishing Lake10 August 2009024 m Secchi depth118 μgL chlorophyll
What is a bloom
bull World Health Organization
bull httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Chorus and Bartram 1999
WHO ThresholdsCell density ge 20000 cellsmlChlorophyll ge 10 microgLMicrocystin drinking water ge1 microgL
What is a bloom
bull World Health Organization
bull US Environmental Protection Agency
bull httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
USEPA drinking water advisoriesmicrocystin
03 microgL children 0-6yrs16 microgL gt6yrs
cylindrospermopsin07 microgL children 0-6yrs30 microgL gt6yrs
What is a bloom
bull World Health Organization
bull US Environmental Protection Agency
bull Carlson Trophic State Index (TSI)bull widely used classification scheme
bull based on transparency (Secchi depth) chlorophyll a total phosphorus to relate to algal biomass typically during the summer
bull scale from 0 ndash 110 increase of 10 TSI units = 2x algal biomassbull TSI useful for comparing lakes within a region and for assessing
changes in trophic status over timebull httpaslonetlotocvol_22issue_20361pdf
What is a bloom
TSI values vs water quality
lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year
40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery
50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo
60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses
gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses
Carlson 1977 Limnology and Oceanography
TSI values
Oligotrophic Mesotrophic Eutrophic Hypereutrophic
Trophic state index
Secchi depth(m)
Chlorophyll(microgL)
Total Phosphorus
(microgL)
NOT A BLOOM
BLOOM
Carlson 1977 Limnology and Oceanography
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
What is a bloom
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Lakesidemonitoringapproaches
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Lake Fox16 January 2016
03 m Secchi depth
bull Important targetsbull Toxigenic cyanobacteria
bull Toxins (cell-bound andor dissolved)
bull Poor transparency
MicrocystisChroococcus Planktothrix
Cylindrospermopsis
Anabaena
Merismopedia Lyngbya Coelosphaerium
How do we quantify cyanobacterial abundance
Huge size range
Kalff 2002 Limnology
Algal group Length (microm)
PicoplanktonNanoplanktonMicroplanktonMesoplankton
02-2020-3030-200
200-20000
Burj Khalifa in Dubai (830m)
Picoplankton Mesoplankton
100000xdifference
Human (2m)
Stacked 240x to reach 200000m
Common techniques for enumerating phytoplankton
Hydrobios settling chambers
High performanceliquid chromatography
Flow CAM imaging particle analyzer
Quantitative PCR
Benchtop fluorometerTurner Designs Trilogy
Field sondeHydrolab minisonde
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Auburn Univ pond7 August 2009
What is a bloom
Fayette County Fishing Lake10 August 2009024 m Secchi depth118 μgL chlorophyll
What is a bloom
bull World Health Organization
bull httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Chorus and Bartram 1999
WHO ThresholdsCell density ge 20000 cellsmlChlorophyll ge 10 microgLMicrocystin drinking water ge1 microgL
What is a bloom
bull World Health Organization
bull US Environmental Protection Agency
bull httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
USEPA drinking water advisoriesmicrocystin
03 microgL children 0-6yrs16 microgL gt6yrs
cylindrospermopsin07 microgL children 0-6yrs30 microgL gt6yrs
What is a bloom
bull World Health Organization
bull US Environmental Protection Agency
bull Carlson Trophic State Index (TSI)bull widely used classification scheme
bull based on transparency (Secchi depth) chlorophyll a total phosphorus to relate to algal biomass typically during the summer
bull scale from 0 ndash 110 increase of 10 TSI units = 2x algal biomassbull TSI useful for comparing lakes within a region and for assessing
changes in trophic status over timebull httpaslonetlotocvol_22issue_20361pdf
What is a bloom
TSI values vs water quality
lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year
40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery
50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo
60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses
gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses
Carlson 1977 Limnology and Oceanography
TSI values
Oligotrophic Mesotrophic Eutrophic Hypereutrophic
Trophic state index
Secchi depth(m)
Chlorophyll(microgL)
Total Phosphorus
(microgL)
NOT A BLOOM
BLOOM
Carlson 1977 Limnology and Oceanography
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
What is a bloom
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Lakesidemonitoringapproaches
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Lake Fox16 January 2016
03 m Secchi depth
bull Important targetsbull Toxigenic cyanobacteria
bull Toxins (cell-bound andor dissolved)
bull Poor transparency
MicrocystisChroococcus Planktothrix
Cylindrospermopsis
Anabaena
Merismopedia Lyngbya Coelosphaerium
How do we quantify cyanobacterial abundance
Huge size range
Kalff 2002 Limnology
Algal group Length (microm)
PicoplanktonNanoplanktonMicroplanktonMesoplankton
02-2020-3030-200
200-20000
Burj Khalifa in Dubai (830m)
Picoplankton Mesoplankton
100000xdifference
Human (2m)
Stacked 240x to reach 200000m
Common techniques for enumerating phytoplankton
Hydrobios settling chambers
High performanceliquid chromatography
Flow CAM imaging particle analyzer
Quantitative PCR
Benchtop fluorometerTurner Designs Trilogy
Field sondeHydrolab minisonde
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Fayette County Fishing Lake10 August 2009024 m Secchi depth118 μgL chlorophyll
What is a bloom
bull World Health Organization
bull httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Chorus and Bartram 1999
WHO ThresholdsCell density ge 20000 cellsmlChlorophyll ge 10 microgLMicrocystin drinking water ge1 microgL
What is a bloom
bull World Health Organization
bull US Environmental Protection Agency
bull httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
USEPA drinking water advisoriesmicrocystin
03 microgL children 0-6yrs16 microgL gt6yrs
cylindrospermopsin07 microgL children 0-6yrs30 microgL gt6yrs
What is a bloom
bull World Health Organization
bull US Environmental Protection Agency
bull Carlson Trophic State Index (TSI)bull widely used classification scheme
bull based on transparency (Secchi depth) chlorophyll a total phosphorus to relate to algal biomass typically during the summer
bull scale from 0 ndash 110 increase of 10 TSI units = 2x algal biomassbull TSI useful for comparing lakes within a region and for assessing
changes in trophic status over timebull httpaslonetlotocvol_22issue_20361pdf
What is a bloom
TSI values vs water quality
lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year
40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery
50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo
60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses
gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses
Carlson 1977 Limnology and Oceanography
TSI values
Oligotrophic Mesotrophic Eutrophic Hypereutrophic
Trophic state index
Secchi depth(m)
Chlorophyll(microgL)
Total Phosphorus
(microgL)
NOT A BLOOM
BLOOM
Carlson 1977 Limnology and Oceanography
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
What is a bloom
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Lakesidemonitoringapproaches
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Lake Fox16 January 2016
03 m Secchi depth
bull Important targetsbull Toxigenic cyanobacteria
bull Toxins (cell-bound andor dissolved)
bull Poor transparency
MicrocystisChroococcus Planktothrix
Cylindrospermopsis
Anabaena
Merismopedia Lyngbya Coelosphaerium
How do we quantify cyanobacterial abundance
Huge size range
Kalff 2002 Limnology
Algal group Length (microm)
PicoplanktonNanoplanktonMicroplanktonMesoplankton
02-2020-3030-200
200-20000
Burj Khalifa in Dubai (830m)
Picoplankton Mesoplankton
100000xdifference
Human (2m)
Stacked 240x to reach 200000m
Common techniques for enumerating phytoplankton
Hydrobios settling chambers
High performanceliquid chromatography
Flow CAM imaging particle analyzer
Quantitative PCR
Benchtop fluorometerTurner Designs Trilogy
Field sondeHydrolab minisonde
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
bull World Health Organization
bull httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Chorus and Bartram 1999
WHO ThresholdsCell density ge 20000 cellsmlChlorophyll ge 10 microgLMicrocystin drinking water ge1 microgL
What is a bloom
bull World Health Organization
bull US Environmental Protection Agency
bull httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
USEPA drinking water advisoriesmicrocystin
03 microgL children 0-6yrs16 microgL gt6yrs
cylindrospermopsin07 microgL children 0-6yrs30 microgL gt6yrs
What is a bloom
bull World Health Organization
bull US Environmental Protection Agency
bull Carlson Trophic State Index (TSI)bull widely used classification scheme
bull based on transparency (Secchi depth) chlorophyll a total phosphorus to relate to algal biomass typically during the summer
bull scale from 0 ndash 110 increase of 10 TSI units = 2x algal biomassbull TSI useful for comparing lakes within a region and for assessing
changes in trophic status over timebull httpaslonetlotocvol_22issue_20361pdf
What is a bloom
TSI values vs water quality
lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year
40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery
50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo
60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses
gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses
Carlson 1977 Limnology and Oceanography
TSI values
Oligotrophic Mesotrophic Eutrophic Hypereutrophic
Trophic state index
Secchi depth(m)
Chlorophyll(microgL)
Total Phosphorus
(microgL)
NOT A BLOOM
BLOOM
Carlson 1977 Limnology and Oceanography
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
What is a bloom
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Lakesidemonitoringapproaches
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Lake Fox16 January 2016
03 m Secchi depth
bull Important targetsbull Toxigenic cyanobacteria
bull Toxins (cell-bound andor dissolved)
bull Poor transparency
MicrocystisChroococcus Planktothrix
Cylindrospermopsis
Anabaena
Merismopedia Lyngbya Coelosphaerium
How do we quantify cyanobacterial abundance
Huge size range
Kalff 2002 Limnology
Algal group Length (microm)
PicoplanktonNanoplanktonMicroplanktonMesoplankton
02-2020-3030-200
200-20000
Burj Khalifa in Dubai (830m)
Picoplankton Mesoplankton
100000xdifference
Human (2m)
Stacked 240x to reach 200000m
Common techniques for enumerating phytoplankton
Hydrobios settling chambers
High performanceliquid chromatography
Flow CAM imaging particle analyzer
Quantitative PCR
Benchtop fluorometerTurner Designs Trilogy
Field sondeHydrolab minisonde
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
bull World Health Organization
bull US Environmental Protection Agency
bull httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
USEPA drinking water advisoriesmicrocystin
03 microgL children 0-6yrs16 microgL gt6yrs
cylindrospermopsin07 microgL children 0-6yrs30 microgL gt6yrs
What is a bloom
bull World Health Organization
bull US Environmental Protection Agency
bull Carlson Trophic State Index (TSI)bull widely used classification scheme
bull based on transparency (Secchi depth) chlorophyll a total phosphorus to relate to algal biomass typically during the summer
bull scale from 0 ndash 110 increase of 10 TSI units = 2x algal biomassbull TSI useful for comparing lakes within a region and for assessing
changes in trophic status over timebull httpaslonetlotocvol_22issue_20361pdf
What is a bloom
TSI values vs water quality
lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year
40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery
50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo
60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses
gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses
Carlson 1977 Limnology and Oceanography
TSI values
Oligotrophic Mesotrophic Eutrophic Hypereutrophic
Trophic state index
Secchi depth(m)
Chlorophyll(microgL)
Total Phosphorus
(microgL)
NOT A BLOOM
BLOOM
Carlson 1977 Limnology and Oceanography
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
What is a bloom
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Lakesidemonitoringapproaches
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Lake Fox16 January 2016
03 m Secchi depth
bull Important targetsbull Toxigenic cyanobacteria
bull Toxins (cell-bound andor dissolved)
bull Poor transparency
MicrocystisChroococcus Planktothrix
Cylindrospermopsis
Anabaena
Merismopedia Lyngbya Coelosphaerium
How do we quantify cyanobacterial abundance
Huge size range
Kalff 2002 Limnology
Algal group Length (microm)
PicoplanktonNanoplanktonMicroplanktonMesoplankton
02-2020-3030-200
200-20000
Burj Khalifa in Dubai (830m)
Picoplankton Mesoplankton
100000xdifference
Human (2m)
Stacked 240x to reach 200000m
Common techniques for enumerating phytoplankton
Hydrobios settling chambers
High performanceliquid chromatography
Flow CAM imaging particle analyzer
Quantitative PCR
Benchtop fluorometerTurner Designs Trilogy
Field sondeHydrolab minisonde
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
bull World Health Organization
bull US Environmental Protection Agency
bull Carlson Trophic State Index (TSI)bull widely used classification scheme
bull based on transparency (Secchi depth) chlorophyll a total phosphorus to relate to algal biomass typically during the summer
bull scale from 0 ndash 110 increase of 10 TSI units = 2x algal biomassbull TSI useful for comparing lakes within a region and for assessing
changes in trophic status over timebull httpaslonetlotocvol_22issue_20361pdf
What is a bloom
TSI values vs water quality
lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year
40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery
50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo
60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses
gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses
Carlson 1977 Limnology and Oceanography
TSI values
Oligotrophic Mesotrophic Eutrophic Hypereutrophic
Trophic state index
Secchi depth(m)
Chlorophyll(microgL)
Total Phosphorus
(microgL)
NOT A BLOOM
BLOOM
Carlson 1977 Limnology and Oceanography
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
What is a bloom
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Lakesidemonitoringapproaches
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Lake Fox16 January 2016
03 m Secchi depth
bull Important targetsbull Toxigenic cyanobacteria
bull Toxins (cell-bound andor dissolved)
bull Poor transparency
MicrocystisChroococcus Planktothrix
Cylindrospermopsis
Anabaena
Merismopedia Lyngbya Coelosphaerium
How do we quantify cyanobacterial abundance
Huge size range
Kalff 2002 Limnology
Algal group Length (microm)
PicoplanktonNanoplanktonMicroplanktonMesoplankton
02-2020-3030-200
200-20000
Burj Khalifa in Dubai (830m)
Picoplankton Mesoplankton
100000xdifference
Human (2m)
Stacked 240x to reach 200000m
Common techniques for enumerating phytoplankton
Hydrobios settling chambers
High performanceliquid chromatography
Flow CAM imaging particle analyzer
Quantitative PCR
Benchtop fluorometerTurner Designs Trilogy
Field sondeHydrolab minisonde
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
TSI values vs water quality
lt40 Oligotrophic clear water high hypolimnetic O2 year-round but possible anoxia in the deeper hypolimnion part of year
40-50 Mesotrophic moderately clear water possible hypolimnetic anoxia in summer andor under ice Fully supportive of all swimmable aesthetic uses possible cold-water fishery
50-60 Mildly eutrophic decreased secchi anoxic hypolimnion possible macrophyte ldquoproblemsrdquo warm-water fishery supportive of all swimmable aesthetic uses but ldquothreatenedrdquo
60-70 blue-green algal dominance with scums possible extensive macrophyte problems not supportive of all beneficial uses
gt70 Heavy blooms and scums in summer likely dense ldquoweedrdquo beds hypereutrophic possible fish kills fewer plant beds due to high algae not supportive of many beneficial uses
Carlson 1977 Limnology and Oceanography
TSI values
Oligotrophic Mesotrophic Eutrophic Hypereutrophic
Trophic state index
Secchi depth(m)
Chlorophyll(microgL)
Total Phosphorus
(microgL)
NOT A BLOOM
BLOOM
Carlson 1977 Limnology and Oceanography
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
What is a bloom
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Lakesidemonitoringapproaches
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Lake Fox16 January 2016
03 m Secchi depth
bull Important targetsbull Toxigenic cyanobacteria
bull Toxins (cell-bound andor dissolved)
bull Poor transparency
MicrocystisChroococcus Planktothrix
Cylindrospermopsis
Anabaena
Merismopedia Lyngbya Coelosphaerium
How do we quantify cyanobacterial abundance
Huge size range
Kalff 2002 Limnology
Algal group Length (microm)
PicoplanktonNanoplanktonMicroplanktonMesoplankton
02-2020-3030-200
200-20000
Burj Khalifa in Dubai (830m)
Picoplankton Mesoplankton
100000xdifference
Human (2m)
Stacked 240x to reach 200000m
Common techniques for enumerating phytoplankton
Hydrobios settling chambers
High performanceliquid chromatography
Flow CAM imaging particle analyzer
Quantitative PCR
Benchtop fluorometerTurner Designs Trilogy
Field sondeHydrolab minisonde
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
TSI values
Oligotrophic Mesotrophic Eutrophic Hypereutrophic
Trophic state index
Secchi depth(m)
Chlorophyll(microgL)
Total Phosphorus
(microgL)
NOT A BLOOM
BLOOM
Carlson 1977 Limnology and Oceanography
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
What is a bloom
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Lakesidemonitoringapproaches
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Lake Fox16 January 2016
03 m Secchi depth
bull Important targetsbull Toxigenic cyanobacteria
bull Toxins (cell-bound andor dissolved)
bull Poor transparency
MicrocystisChroococcus Planktothrix
Cylindrospermopsis
Anabaena
Merismopedia Lyngbya Coelosphaerium
How do we quantify cyanobacterial abundance
Huge size range
Kalff 2002 Limnology
Algal group Length (microm)
PicoplanktonNanoplanktonMicroplanktonMesoplankton
02-2020-3030-200
200-20000
Burj Khalifa in Dubai (830m)
Picoplankton Mesoplankton
100000xdifference
Human (2m)
Stacked 240x to reach 200000m
Common techniques for enumerating phytoplankton
Hydrobios settling chambers
High performanceliquid chromatography
Flow CAM imaging particle analyzer
Quantitative PCR
Benchtop fluorometerTurner Designs Trilogy
Field sondeHydrolab minisonde
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
What is a bloom
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Lakesidemonitoringapproaches
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Lake Fox16 January 2016
03 m Secchi depth
bull Important targetsbull Toxigenic cyanobacteria
bull Toxins (cell-bound andor dissolved)
bull Poor transparency
MicrocystisChroococcus Planktothrix
Cylindrospermopsis
Anabaena
Merismopedia Lyngbya Coelosphaerium
How do we quantify cyanobacterial abundance
Huge size range
Kalff 2002 Limnology
Algal group Length (microm)
PicoplanktonNanoplanktonMicroplanktonMesoplankton
02-2020-3030-200
200-20000
Burj Khalifa in Dubai (830m)
Picoplankton Mesoplankton
100000xdifference
Human (2m)
Stacked 240x to reach 200000m
Common techniques for enumerating phytoplankton
Hydrobios settling chambers
High performanceliquid chromatography
Flow CAM imaging particle analyzer
Quantitative PCR
Benchtop fluorometerTurner Designs Trilogy
Field sondeHydrolab minisonde
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
What is a bloom
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Lakesidemonitoringapproaches
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Lake Fox16 January 2016
03 m Secchi depth
bull Important targetsbull Toxigenic cyanobacteria
bull Toxins (cell-bound andor dissolved)
bull Poor transparency
MicrocystisChroococcus Planktothrix
Cylindrospermopsis
Anabaena
Merismopedia Lyngbya Coelosphaerium
How do we quantify cyanobacterial abundance
Huge size range
Kalff 2002 Limnology
Algal group Length (microm)
PicoplanktonNanoplanktonMicroplanktonMesoplankton
02-2020-3030-200
200-20000
Burj Khalifa in Dubai (830m)
Picoplankton Mesoplankton
100000xdifference
Human (2m)
Stacked 240x to reach 200000m
Common techniques for enumerating phytoplankton
Hydrobios settling chambers
High performanceliquid chromatography
Flow CAM imaging particle analyzer
Quantitative PCR
Benchtop fluorometerTurner Designs Trilogy
Field sondeHydrolab minisonde
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
bull Go samplebull Integrated andor surface
samples
bull Whole water samples
bull Secchi depth
What is a bloom
Lake Fox16 January 2016
03 m Secchi depth
bull Important targetsbull Toxigenic cyanobacteria
bull Toxins (cell-bound andor dissolved)
bull Poor transparency
MicrocystisChroococcus Planktothrix
Cylindrospermopsis
Anabaena
Merismopedia Lyngbya Coelosphaerium
How do we quantify cyanobacterial abundance
Huge size range
Kalff 2002 Limnology
Algal group Length (microm)
PicoplanktonNanoplanktonMicroplanktonMesoplankton
02-2020-3030-200
200-20000
Burj Khalifa in Dubai (830m)
Picoplankton Mesoplankton
100000xdifference
Human (2m)
Stacked 240x to reach 200000m
Common techniques for enumerating phytoplankton
Hydrobios settling chambers
High performanceliquid chromatography
Flow CAM imaging particle analyzer
Quantitative PCR
Benchtop fluorometerTurner Designs Trilogy
Field sondeHydrolab minisonde
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
MicrocystisChroococcus Planktothrix
Cylindrospermopsis
Anabaena
Merismopedia Lyngbya Coelosphaerium
How do we quantify cyanobacterial abundance
Huge size range
Kalff 2002 Limnology
Algal group Length (microm)
PicoplanktonNanoplanktonMicroplanktonMesoplankton
02-2020-3030-200
200-20000
Burj Khalifa in Dubai (830m)
Picoplankton Mesoplankton
100000xdifference
Human (2m)
Stacked 240x to reach 200000m
Common techniques for enumerating phytoplankton
Hydrobios settling chambers
High performanceliquid chromatography
Flow CAM imaging particle analyzer
Quantitative PCR
Benchtop fluorometerTurner Designs Trilogy
Field sondeHydrolab minisonde
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Huge size range
Kalff 2002 Limnology
Algal group Length (microm)
PicoplanktonNanoplanktonMicroplanktonMesoplankton
02-2020-3030-200
200-20000
Burj Khalifa in Dubai (830m)
Picoplankton Mesoplankton
100000xdifference
Human (2m)
Stacked 240x to reach 200000m
Common techniques for enumerating phytoplankton
Hydrobios settling chambers
High performanceliquid chromatography
Flow CAM imaging particle analyzer
Quantitative PCR
Benchtop fluorometerTurner Designs Trilogy
Field sondeHydrolab minisonde
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Common techniques for enumerating phytoplankton
Hydrobios settling chambers
High performanceliquid chromatography
Flow CAM imaging particle analyzer
Quantitative PCR
Benchtop fluorometerTurner Designs Trilogy
Field sondeHydrolab minisonde
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Graham et al 2008 USGS reporthttppubsusgsgovsir20085038pdfSIR2008-5038pdf
Common toxin analytical approaches
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Chorus and Bartram 1999httpappswhointirisbitstream1066542827
10419239308_engpdfua=1
Common toxin analytical approaches
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Outlinebull New HABs monitoring tools
bull CellScope
bull Phycocyanin
bull qPCR
bull Environmental Sample Processor (ESP)
bull Flow cytobots
bull Toxin test strips and tube kits
bull Real-time databull Forecasting websites
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Outlinebull New HABs monitoring tools
httpehpniehsnihgovwp-contentuploads1228ehp122-A206pdf
Seltenrich 2014
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
CellScope
bull httpcellscopeberkeleyedu
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Phycocyanin approaches
Turner Designs Trilogy
Orange module630nm excitation660nm emission
Field sondeHydrolab minisonde
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Phycocyanin benchtop method development
bull Field sampling
ndash September 2012 ndash 16 ponds (pond survey)
Auburn University pond facility
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Pigments vs cell densities
bull Weak to no relationships between pigments and cell densities
ndash Only statistically significant relationship was chlorophyll vs phytoplankton
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Pigments vs biovolumes
bull Strong relationships between pigments and biovolume or only pigments
ndash Large range
ndash Wide diversity of taxa
ndash Chlorophyll good predictor of phycocyanin
Kasinak et al 2015 Journal of Plankton Researchhttpplanktoxfordjournalsorgcontent371248abstract
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
bull qPCRbull Quantifies number of gene copies associated with taxa-
and trait-specific primers and probes
bull Phytoxigenebull CyanoDTec ndash microcystin nodularin
cylindrospermopsin saxitoxin
bull DinoDTec ndash saxitoxin
bull httpwwwphytoxigenecom
bull httpswwwyoutubecomwatchv=o2bYaY1TDLs
qPCR assays
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
bull Automated in situ collection and analyses for water quality and DNA analysis of biological community to identify toxigenic taxa
bull Remotely shares data to improve forecasting models
bull httpwwwnwfscnoaagovresearchdatatechtechespcfm
Environmental Sample Processor (ESP)
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Flow cytobot
bull Automated in situ collection and analyses to identify toxigenic phytoplankters using video flow cytometric and fluorescence technology
bull Remotely shares data to improve forecasting models
bull httpwwwwhoiedumainimaging-flow-cytobot
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Toxin test strips and tube kitsbull Available for several compounds including
microcystin cylindrospermopsin and anatoxin
bull Abraxisbull httpwwwabraxiskitscomproductsalgal-toxins
bull Beacon Analytical Systemsbull httpwwwbeaconkitscomwelcome
Abraxis test strip (05 ndash 50 microgL)Beacon toxin tube kit (03 ndash 50 microgL)
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Toxin test strips and tube kits
bull 2007 EPA National Lakes Assessment
bull 1252 samples for microcystin
bull Range 0-225 microgLbull 68 - undetectable bull 16 - 005-05 microgLbull 5 - 05-10 microgLbull 9 - 10-50 microgLbull 2 - gt5 microgL
bull httpwwwepagovnational-aquatic-resource-surveysdata-national-aquatic-resource-surveys
Hollister et al 2016 F1000Researchhttpf1000researchcomarticles5-151v1
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Real-time data and forecastingbull Remote sensing
bull httpwwwsfeiorgsitesdefaultfilesRick20Stumpf_part2_RS_basicspdf
Stumpf et al 2015 talk
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Real-time data and forecastingbull NOAA Harmful Algal Bloom Operational Forecast
System (HAB-OFS)bull Gulf of Mexico ndash Karenia brevis
bull httphabsosnoaagovbull httptidesandcurrentsnoaagovhab
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Real-time data and forecastingbull National Centers for Coastal Ocean Sciences
(NCCOS) funding research in support of pilot regional HAB forecasts inhellip
bull Pacific Northwest - Pseudo-nitzscha
bull Puget Sound - Alexandrium
bull Gulf of Maine - Alexandrium
bull Southern California - Pseudo-nitzschia
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Real-time data and forecastingbull Great Lakes Observing System (GLOS)
bull Western Lake Erie
bull httphabsglosusmap
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie HABs tracker (forecast days 1-5)
bull httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Real-time data and forecastingbull Great Lakes Environmental Research Lab (GLERL)
bull Western Lake Erie
bull httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Real-time data and forecastingbull Phytoplankton monitoring network
bull NOAA Citizen science outreach to educate public about harmful phytoplankton taxa in coastal areas and Great Lakes region
bull httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
bull httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Real-time data and forecastingbull What about inland lakes
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Real-time data and forecastingbull California Surface Water Ambient Monitoring
Program (SWAMP)bull Uses remote sensing surface water color satellite data to
estimate and forecast HABs in fresh waterbodies
bull httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Real-time data and forecastingbull USEPA NOAA USGS and NASA working together
to develop an android HABs appbull Cyanobacteria Assessment Network (CyAN) mobile appbull Uses MODIS surface water color satellite data to
estimate and forecast HABs
bull httpsdeveloperepagovhab-challengebull httpswwwnalmsorgmediaacuxbeb75c9c-f812-
4753-b888-79864899c6d6
Mattas-Curry et al 2015 LakeLine
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcombloom_network
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
SECCHI DEPTH FORECASTING MODEL
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Real-time data and forecasting
httpwilsonlabcomforecastinghtml
WATER QUALITY FORECASTING MODEL
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Aquaculture pondAlabama August 2008
Contact informationAlan Wilson
Fisheries ndash Auburn University
203 Swingle Hall Auburn AL 36849
wilsonauburnedu 334-246-1120
httpwilsonlabcom
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
PAPERS
Carlson R E 1977 A trophic state index for lakes Limnology and Oceanography 22361-369 httpaslonetlotocvol_22issue_20361pdf
Chorus I and J Bartram 1999 Toxic cyanobacteria in water A guide to their public health consequences monitoring and management httpwwwwhointwater_sanitation_healthresourcesqualitytoxicyanbacten
Doster E M F Chislock J F Roberts J J Kottwitz and A E Wilson 2013 Recognition of an important water quality issue at zoos prevalence and potential threat of toxic cyanobacteria Journal of Zoo and Wildlife Medicine 45174-177 httpwilsonlabcompublications2014_JZWM_Doster_et_alpdf
Graham J L K Loftin A C Keith and M T Meyer 2008 Guidelines for design and sampling for cyanobacterial toxin and taste-and-odor studies in lakes and reservoirs US Geological Survey Scientific Investigations Report 2008-5038 httppubsusgsgovsir20085038pdfSIR2008-5038pdf
Kasinak J-M E B M Holt M F Chislock and A E Wilson 2015 Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume Journal of Plankton Research 37(1)248-257 httpplanktoxfordjournalsorgcontent371248abstract
Mattas-Curry L B A Schaeffer R B Conmy and D J Keith 2015 A space satellite perspective to monitor water quality using your mobile phone LakeLine 35(2)28-31 httpswwwnalmsorgmediaacuxbeb75c9c-f812-4753-b888-79864899c6d6
Rosen B and A St Amand 2015 Field and laboratory guide to freshwater cyanobacteria harmful algal blooms for Native American and Alaska Native communities USGS Report 2015-1164 httppubserusgsgovpublicationofr20151164
Seltenrich N 2014 Keeping tabs on HABs new tools for detecting monitoring and preventing harmful algal blooms Environmental Health Perspectives 122(8)A206-A213 httpehpniehsnihgov122-a206
USEPA 2015 Drinking water health advisories for two cyanobacterial toxins httpswwwepagovnutrient-policy-datadrinking-water-health-advisory-documents
Relevant publications and links
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml
Relevant publications and linksTOOLS
CellScope httpcellscopeberkeleyedu
Cyanobacteria Assessment Network (CyAN) mobile app httpsdeveloperepagovhab-challenge
Environmental Sample Processor httpwwwnwfscnoaagovresearchdatatechtechespcfm
Flow Cytobot httpwwwwhoiedumainimaging-flow-cytobot
Phyto cell phone app httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Phytoxigene httpwwwphytoxigenecom
Toxin strips httpwwwabraxiskitscomproductsalgal-toxins
Toxin tube kits httpwwwbeaconkitscomwelcome
REAL-TIME DATA AND FORECASTING
California Surface Water Ambient Monitoring Program (SWAMP) httpwwwsfeiorgnews_itemsnoaa-remote-sensing-tools-and-application-evaluating-cyanohabs-california-lakes
Great Lakes Environmental Research Lab (GLERL) httpwwwglerlnoaagovresHABs_and_HypoxiahabsTrackerhtml httpwwwglerlnoaagovresHABs_and_HypoxiartMonWLEhtml
Great Lakes Observing System (GLOS) httphabsglosusmap
National Centers for Coastal Ocean Sciences (NCCOS) httpiodlabsucsdedusgiddingsPNWTOXinfoModelhtml httpcoastalsciencenoaagovprojectsdetailkey=148
NOAA Harmful Algal Bloom Operational Forecast System (HAB-OFS) httphabsosnoaagov httptidesandcurrentsnoaagovhab
Phytoplankton monitoring network httpsplaygooglecomstoreappsdetailsid=nameganophytoamphl=en
Wilson A E Cyanobacterial bloom forecasting website httpwilsonlabcomforecastinghtml