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New Data Bank on Toxicological Properties of Chemical Product,

BARIL, Marc.

Commission de la sant6 et de la s6curit6 du travail du Quebec

C.P. 1056, succursale Desjardins, Montreal, Quebec H5B IC2

One of the functions of the Commission de la Sante et de la s&urite du travail, created by the Quebec Act respecting the Occupational Health & Safety, is to maintain and update a toxicological index. The main purpose or this index is to provide informations related to health and safety regarding the use of industrial and commercial chemical product in establis~ent located in Quebec province.

The design of the system allows, this data bank to treat informations on approximately 60 000 chemical products. 80 000 requests each year.

When fully operative, this system will be able to process

As the system became available to the users 700 responses on toxicological information were emitted monthly from the 1 200 requests received. the first year of operation.

These data were compiled during During the same perSod of time, technical iflfor~ations on

10 000 chemical products of with 35 % concern pure substance, 40 % involve mixture and 25 % related to trade names of unknown composition, have been collected.

Data concerning the evolution of the data bank, the number of requests, and the number of responses will be presented.

How the information is treated in the system and transfer to the users, and how these info~ations are used will be the subject of the discussion.

Mutagenic Activities of Phthalic Acid Esters, DEHP and MEHP

Y. NAKANURA', I. TOMIT& N. INUP, AND K. TUTIRwA3 'Shizuoka College of Pharmaceutical Sciences 2-2-I Oshika, Shizuoka(422) Japan 16iol. Res. Center, Japan Tobacco & Salt PubJ. Corp., Hatano, Kanagawa(257) Japan 3National Institute of Genetics, 1,111 Yata, Mishlma(411) Japan

DEHP. di-(2-~thyl~xyl)phthalate, is the widely used plasticizer and has been reported to be mutagenic in the dominant lethal assay in nice(Sfngh et at.,l978) and caused liver tumors in the carcinogenicity tests in both rats and mice(NTP, 1981).

. . AS fi;r zn uztro

mutagenicity tests on OEHP, however, mostly negative results have been obtained in various systems employed. We have previously reported that MEHP induced the DNA-damaging/mutagenic activity to &uriZlua oubtiZie(Rec-assay), &Zmsn&Za

T h&m&urn TAIDO(plate assay) and

Esohepichia e&i B/r WP2(spot test) (Yagi et al., 1978 . The present investjgation was conducted to determine the mutagenic potential of both

DEHP and MEHP(&z uiti and in &no) and the following results were obtained. 1) DEHP(1 mg/disk, +S9 mix) and MEHP(5DO pgfdisk) showed the Do-damaging effects in Rec-

assay using B. subtiZZs H17 and M45. 2) MEHP(700-1400 us/ml) increased the mutation frequencies in e. coZi B/r WP2 and S.

typhinncriMt TA 100. 3) MEHp(SD pg/ml, 1.0 mg/ml and 1.5 mg/ml) induced sister chromatid exchanges, chromosomal

aberration and B-AGr mutation in Y79 Chinese hamster cells. 4) DEHP(l.5 g/kg) and ~~(0.15 g/kg) applied p.o. to pregnant Syrian golden hamster

elevated the frequencies of chr~s~t aberration, S-AGr station and ~rphological transfonoation in its embryonic cells.

5) DEHP(5W ag/kg) adnfnistered i.p. showed no somatic mutation in the spot test with mice (KY6 female X PW male) while MEHP(25 , 50 rag/kg) did produce the differenti:;;;;dspots.

6) Low level of MHP(3.9 pg/ml) and NEHP(O.Zfl ug/ml) reduced the 4-NQO/MNNG nutagenesfs to about 50% of the original activities in V79 Chinese hamster cells.

In conclusfon, the aa&agenic potential of DEHP should be carefully evaluated on the basis that it produced dual effects depending on its dose applied in the experimental systems-