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Network Biology Approaches to
Bacterial Infections
James J. Collins
Howard Hughes Medical Institute
Dept of Biomedical Engineering & Center of Synthetic Biology
Boston University
Wyss Institute for Biologically Inspired Engineering
Harvard University
Microbial Network Biology and Antibiotic Research
Focus: use network biology approaches to study the actions of
antibiotics and mechanisms underlying the emergence of resistance
Goal: utilize insights gained from these studies to enhance our
existing antibiotic arsenal and treat resistant bacterial infections
Bacterial Persisters: Chronic and Recurrent Infections
Metabolite-Enabled Eradication of Bacterial Persisters
KR Allison et al., Nature, 2011
Metabolic Screen to Potentiate Aminoglycosides
KR Allison et al., Nature, 2011
Metabolic Screen to Potentiate Aminoglycosides
KR Allison et al., Nature, 2011
Metabolite-Enabled Aminoglycoside Uptake
KR Allison et al., Nature, 2011
Metabolite-Enabled Aminoglycoside Uptake and Killing Require PMF
KR Allison et al., Nature, 2011
aminoglycoside uptake and persister
eradication require PMF
Electron Transport Chain is Important to Persister Eradication
KR Allison et al., Nature, 2011
Metabolite-Enabled Eradication of Bacterial Persisters
KR Allison et al., Nature, 2011
Metabolite-Enabled Treatment of Bacterial Biofilms
KR Allison et al., Nature, 2011
Metabolite-Enabled Treatment of Catheter-Associated UTI
KR Allison et al., Nature, 2011
Engineered Bacteriophage as Antibacterial Adjuvants
TK Lu and JJ Collins, PNAS, 2009
Synthetic Probiotics for Detecting and Treating Infections
Lactobacillus
Functionalize the Microbiome Using Engineered Bacteriophage