NemOutNemOutTMTM

A new nematicide in A new nematicide in development for control development for control of reniform and other of reniform and other nematodes in cottonnematodes in cotton

Timothy JohnsonTimothy JohnsonPlato Industries, Ltd.Plato Industries, Ltd.Houston, TXHouston, TX

Difficulties in Difficulties in managing nematodes managing nematodes in cottonin cotton Multiple species - root knot, reniform, Multiple species - root knot, reniform,

Columbia lanceColumbia lance Soil pests are generally more difficult to Soil pests are generally more difficult to

manage than foliar pestsmanage than foliar pests Nematodes are difficult to detect and Nematodes are difficult to detect and

costly to samplecostly to sample Distributions are typically unevenDistributions are typically uneven Yield losses are often contingent upon Yield losses are often contingent upon

additional stress factorsadditional stress factors

Nematode Nematode management management strategiesstrategies Crop rotation – economic and Crop rotation – economic and

biological limitationsbiological limitations

Host plant resistance – slow to Host plant resistance – slow to develop and bring to marketdevelop and bring to market

Chemical and/or biological Chemical and/or biological interventionintervention

Nematode Nematode management management strategies - chemicalstrategies - chemical Temik® 15G – long time soil Temik® 15G – long time soil

insecticide/nematicideinsecticide/nematicide AVICTA® Complete Pak – includes AVICTA® Complete Pak – includes

abamectin for nematode controlabamectin for nematode control Vydate® – layby applicationVydate® – layby application Telone® - soil fumigantTelone® - soil fumigant

Nematode Nematode management management strategies - biologicalstrategies - biological

N-HibitN-HibitTMTM – induces plant defense – induces plant defense mechanismsmechanisms

NemOutNemOutTMTM

Pathogenic fungus Pathogenic fungus Paecilomyces Paecilomyces lilacinuslilacinus strain 251 strain 251

Primarily an egg parasite with Primarily an egg parasite with lesser activity on juvenile and lesser activity on juvenile and adult nematodesadult nematodes

NemOutNemOutTMTM

Manufactured by ProPhyta Biologischer Manufactured by ProPhyta Biologischer Pflanzenschutz GmbH of Poel, GermanyPflanzenschutz GmbH of Poel, Germany

Active ingredient is EPA registered for use on Active ingredient is EPA registered for use on bananas, fruiting vegetables and turf.bananas, fruiting vegetables and turf.

NemOut is a new formulation specifically for NemOut is a new formulation specifically for cotton and peanuts and is being developed cotton and peanuts and is being developed by Plato Industries, Ltd. of Houston, TXby Plato Industries, Ltd. of Houston, TX

NemOutNemOutTM TM -- mode of actionmode of action

Paecilomyces lilacinusPaecilomyces lilacinus mycelium infecting nematode eggs. mycelium infecting nematode eggs.

©Rita Holland©Rita Holland

NemOutNemOutTM TM – – development development objectives for cotton and peanutsobjectives for cotton and peanuts

Use RatesUse Rates Water volumesWater volumes Application timing and Application timing and

methodologymethodology Compatibility with fungicidesCompatibility with fungicides

NemOutNemOutTM TM – 2006 field – 2006 field trialstrials

Cotton – Mississippi deltaCotton – Mississippi delta

Peanuts – Alabama and FloridaPeanuts – Alabama and Florida

Untreated2 lbs NemOut IF4 lbs NemOut IF8 lbs NemOut IFTemik 15G 5 lb IFAvicta C-P

DREC, MSU – Trial 1

Cooperator: Dr. J. Smith

Original 1X formulation

200 ft. plots x 4 rows6 repsRCBDPlanted 4/19Harvested 9/20

100 0 100 Feet

F9sw_veris_deep.shp20.17 - 29.9629.96 - 36.9936.99 - 42.6942.69 - 47.0647.06 - 50.6250.62 - 53.6453.64 - 56.3856.38 - 59.1759.17 - 62.3662.36 - 66.0366.03 - 70.5970.59 - 75.8775.87 - 81.7581.75 - 88.6888.68 - 98.09

Clip3.shpClip4.shp

Measuring Soil Electrical Conductivity (EC)

Veris DataTrial 1

100 0 100 Feet

F9sw_nem060421.shp0 - 385386 - 725726 - 876877 - 10011002 - 11141115 - 12181219 - 13151316 - 14141415 - 15171518 - 16261627 - 17441745 - 18681869 - 19981999 - 21352136 - 22822283 - 24492450 - 26812682 - 30283029 - 35313532 - 43494350 - 5444

Clip3.shpClip4.shp

Pre-treatment

100 0 100 Feet

100 0 100 Feet

F9sw_nem060421.shp0 - 385386 - 725726 - 876877 - 10011002 - 11141115 - 12181219 - 13151316 - 14141415 - 15171518 - 16261627 - 17441745 - 18681869 - 19981999 - 21352136 - 22822283 - 24492450 - 26812682 - 30283029 - 35313532 - 43494350 - 5444

Clip3.shpClip4.shp

Post-treatment

100 0 100 Feet

NemOut -Trial 1Reniform nematodes per 500 cc soil

Square Counts

DREC,MSU

Trial 1

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

No nematicide NemOut WG 2 lb/a IF Temik 15G 5 lb/a IF AVICTA Complete Pak

Squ

ares

per

pla

nt

19-Jul

26-Jul

9-Aug

NemOutNemOutTM TM – trial 1 – trial 1 harvestharvestGPS linked picker with continuous read out yield monitorGPS linked picker with continuous read out yield monitor

Untreated0.2 lbs NemOut IF0.4 lbs NemOut IF0.8 lbs NemOut IFTemik 15G 5 lb. IF

Avicta C-P

DREC, MSU – Trial 2

Cooperator: Dr. G. Scuimbato

New 10x formulation

NemOut Seed Trt.1 lb/100 CWT2 lb/100 CWT2 lb/CWT + Temik2 lb/CWT + Avicta

45 ft. plots x 4 rows5 repsRCBDPlanted 5/4Harvested 9/26

Preparing seed with NemOut

Injecting seed with conidia.

Treated seed.

Mixing conidia.

Seed Cotton in LB/AcreDREC, MSU

Trial 2

3000

3100

3200

3300

3400

3500

3600

3700

3800

3900

LB/Acre

Seed Cotton in LB/AcreTrial 2

3100

3200

3300

3400

3500

3600

3700

3800

No nematicide AVICTA CompletePak

Temik 15G @ 5 lb/aIF

NemOut WP 0.2 lb/aIF+2 lb/CWT seed trt.

NemOut WP at 1lb/CWT seed trt.

NemOut WP at 2lb/CWT seed trt.

NemOut WP at 2lb/CWT + Temik 5 lb

IF

NemOut WP 0.2 lb/aIF + AVICTA

LB/Acre

NemOutNemOutTM TM – peanut trial– peanut trial

NemOut Peanut Trial - FloridaYield in pounds per acre

2400

2500

2600

2700

2800

2900

3000

3100

3200

NemOut 0.2 lb/acreIF and peg

NemOut 0.4 lb/acreIF and peg

Temik 10 lb./acre IFand peg

Untreated

LB

S p

er a

cre

Cooperator: Dr. J. Rich

NemOutNemOutTM TM – peanut trial– peanut trial

NemOut Peanut Trial - AlabamaYield in LBS per acre

5200

5300

5400

5500

5600

5700

5800

5900

6000

6100

NemOut WP0.1 lb/acre IF

NemOut WP0.1 lb/acre IF

and peg

NemOut WP0.2 lb/acre IF

NemOut WP0.2 lb/acre IF

and peg

NemOut WP0.4 lb/acre IF

NemOut WP0.4 lb/acreIF

and peg

NemOut WP0.8 lb/acre IF

Temik 15G 10lb/acre IF

Cooperator: Dr. K. Lawrence

NemOutNemOutTM TM – future – future directiondirection

Complete compatibility studies with Complete compatibility studies with seed applied fungicidesseed applied fungicides

Expand geographic area for cotton Expand geographic area for cotton trials to include both root knot and trials to include both root knot and reniform infestationsreniform infestations

Focus on application methodologies to Focus on application methodologies to include application through irrigation include application through irrigation systems and hopper box applicationssystems and hopper box applications

NemOutNemOutTM TM – future – future directiondirection

Proceed with evaluating use rates Proceed with evaluating use rates that are economically competitive that are economically competitive with commercial standardswith commercial standards

Complete the registration process Complete the registration process to allow large-scale evaluations in to allow large-scale evaluations in 20072007

AcknowledgementsAcknowledgements

Mississippi State University – DRECMississippi State University – DREC

Dr. Jim SmithDr. Jim Smith

Dr. Gabe SciumbatoDr. Gabe Sciumbato

Dr. Pat English Dr. Pat English

Boisee StokesBoisee Stokes

Troy GibsonTroy Gibson

University of Florida – QuincyUniversity of Florida – Quincy

Dr. Jim RichDr. Jim Rich

Auburn UniversityAuburn University

Dr. Kathy LawrenceDr. Kathy Lawrence