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NE Regional Lobster Sample Repository: Data Collection and Initial Findings of the
Lobster Health Coalition
Sponsored by the
with a grant from NOAA
Primary Researchers• Deborah A. Bouchard – UMaine Aquatic Animal Health Lab
• Anne B. Lichtenwalner - UMaine Aquatic Animal Health Lab
• Kathy M. Castro - University of Rhode Island
• Mark D. Fast - Stony Brook University
• Roxanna M. Smolowitz - Roger Williams University
Lobster Health Coalition
• Summer 2009: – Target: 1500 lobsters from Ventless Trap
surveys in 3 general NE regions• Sites:
– Maine – Rhode Island/Massachusetts– Long Island Sound (LIS)
• Numbers: – Maine: 378– RI/MASS: 400– LIS: 154 (VTS animals returned to stock)
Data Collection
Processing Samples
• Logged in:–General
observations–Size–Vigor/mortality–Visible shell
lesions
Data Processing
– Hemolymph • Direct observation for parasites• Culture• Pelleted hemocytes for cDNA
Data Processing
• Archival information:– Region/GIS/Date of collection
• Hemocyte RNA: – High proportion with high sample integrity– Re-precipitating/cleaning
• Histology:– Eyestalk, antennal gland, hepatopancreas,
heart, stomach, muscle, gill, gonad, nerve, intestine, shell (formalin fixed, some sectioned)
• Toxicology– Hepatopancreas (frozen)
Sample Repository
• Total= 932– Maine: 378– LIS: 154– RI/MASS: 400
• Histology prepared/read (R. Smolowitz)– Maine: 35– LIS: 15– RI/MASS: 42
Sample Repository
.
Shell Disease vs Bacteremia
ME LIS RI/MASS0
20
40
60Shell DiseaseBacteremia
Source
% o
f L
ive
Lo
bst
ers
• Gaffkemia: Aerococcus viridans– None detected
• Bumper car disease: 1– Anophryoides haemophilia
Bacteremia
• Vibrio spp.• V. splendidus• V. aestaurianus• V. alginolyticus• V. carcarias• V. fluvialis• V. furnissii• V. metschnikovii• V. proteolyticus• V. vulnificans• V. harveyii
Isolates Identified
•Photobacterium spp.
•P. indicum•P. damsellae ss damsellae
•Other•Pasteurella multocida•Actinomyces•Aeromonas•Listonella anguillarum
• Website development• Grants from
–Atlantic Lobster Sustainability Foundation–Margaret E. Burnham Charitable Trust–Northeast AgEnhancement
• Repository extension
• Expand Lobster Health Coalition
Further Work
Questions welcomed.
Thank you.
The Value of “Value-Added”
Sustaining our Lobstermen