Natural Materials for Dural Replacement and Neuroprotection

7/23/2019 Natural Materials for Dural Replacement and Neuroprotection

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Vanessa AguilarProject PlanOctober 27, 2010

Natural Materials for DuralReplacement and Neuroprotection


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Dural Replacement Therapy Needs

Dura lesioncomplications:

Meningitis Cerebral spinal fui

lea! Pseuomeningocele Arac"noiitis #piural abscess

Current uralreplacementmar!et

$ore%&e' (eP&)#* +europatc" (polesteruret"ane*

Duragen (Collagen* Dura-eal (P#$%basespra*

&isseel ()ibrin.trombinsolutions*

Preclue (P&)#.elastomericfuoiropolmer*

/istor o uralreplacement 13 4rst ural

replacement1

Mi 05s 'enograp"an allograp" 6ereuse

-ince 705s biosnt"eticgrat 6ere inestigate

189 o spinal surgeriesreuires a uralreplacementtec"nolog2

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Anatomical Dural Overview

"ttp:..members;co';net.injections.images.esi?images.roots;jpg

+arotam P; et al, -pine 2008

-tenel > et al;= +eurosurg 200,

>un@a et al, Anest" Analg, 1


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Dural >eplacement . Cranial A"esion arriersarrier Deice Dura$en

(Bntegra ie -ciences*-nt"ecel Dura

(-nt"es*Dura-eal(Coiien*

A"erus(/perranc"*

Moel

Description Dura$en.Dura$enPlus is an innoatiematri' esigne topreent periural4brosis an a"esions

Cellulose Emicrobiall gro6ncellulose

P#$ "rogel -nt"etic surgicalsealant E P#$"rogel blen

Properties Collagen baseAe cellulose laeror suturable

perormance

&"ic!, er strongs"eets o cellulose

1009 snt"eticFater%tig"t sealantto be applie uring

cranial or spinalsurgeries or urarepair

C# approe orspinal applications

Aantages )DA approe orneural applications+atural%basematerialioresorbable but

egraation resistant

)DA approe orural replacementan 6oun ressingP"ase BBB clinicaltrials

)DA approe orcranial an spinalsurgeries;Bnjectable an easto use

-pra%use, eas touse

Disaantages

+ot eas to "anle+ot an a"esionbarrierAttracts a"esions

Ver e'pensie&imel to gro6Cannot be gro6nmass%scale

-et up reuire-nt"eticCan beprocoagulant+ere compressionma ocur1

-et up reuire-nt"eticCan beprocoagulant

1; -potnit@, F an ur!s, -; &ransusion;200


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Plan of WorkGOA! To develop composite" dual#functionin$ materials that wouldserve to encoura$e healthy cell $rowth" wound healin$ and inhi%its

post#sur$ical scar tissue formation for neural applications& We aim todevelop an all#in#one product to replace dural tissue as well assupport healthy healin$&

A'M (! Develop andcharacteri)e sutura%leanti#adhesion *lm +

foam

,iocompati%le Non#immuno$enic Non cell#adhesive +

cytoto-ic

'nhi%its proteina%sorption Mechanically ro%ust Waterti$ht + sealin$ Anti#*%rotic

A'M .! Dru$ releasestudies

,iocompati%le /0ective at reducin$

adhesions /ncapsulate aspirin

or i%uprofen

Tuna%le releaserates

A'M 1! Develop %ilayer%iofunctionali)ed 2A#%ased *lm

,iocompati%le ,ioa%sor%a%le Non#immuno$enic Dual functionin$

Re$enerative

Anti#adhesive Mechanically ro%ust 3ost e0ective 3linically si)ed Repositiona%le


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GOA! To develop composite" dual#functionin$ materials that wouldserve to encoura$e healthy cell $rowth" wound healin$ and inhi%its

post#sur$ical scarred tissue formation for neural applications& Weaim to develop an all#in#one product to replace dural tissue as well assupport healty heallin$&

A'M (! Develop andcharacteri)e sutura%leanti#adhesion *lm +

foam

,iocompati%le Non#immuno$enic Non cell#adhesive +

cytoto-ic

'nhi%its proteina%sorption Mechanically ro%ust Waterti$ht + sealin$ Anti#*%rotic

A'M .! Dru$ releasestudies

,iocompati%le /0ective at reducin$

adhesions /ncapsulate aspirin

or i%uprofen

Tuna%le releaserates

A'M 1! Develop %ilayer%iofunctionali)ed 2A#%ased *lm

,iocompati%le ,ioa%sor%a%le Non#immuno$enic Dual functionin$

Re$enerative

Anti#adhesive Mechanically ro%ust 3ost e0ective 3linically si)ed Repositiona%le

Plan of Work


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Material Properties

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Anti 3ell#Adhesion Properties

2; Culture 4broblast cells

G; 1;3 "ours in culture

8; )i' an stain or DAPB;

3; Valiate cell%a"esion . noncell%a"esions

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1; Fell an 4lm


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Results

Alginate Alginate .$M/A

Alginate .$M/A ./A Control&"ere is signi4cant iHerencebet6een control an 4lms (p J

0;003*

0

23

3073

100

123

3ell Adhesion 4tudies

5 3ell Adhesion


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3ytoto-icity1; Culture 4broblastcells

2; -ee cells in P coate &Ccoerslips

8; -tain coerslips 6it"calcein . et"iium to label lie .ea cells;

3; #aluatectoto'icit

8; Fait 28"ours

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3; Place Alginate . /A4lm supernatant on topo cells

G; Place Alginate . /A4lm on cell meium

8; Fait 28"ours


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Results


Control

&"ere is no statistical iHerencebet6een control an 4lms in lie an

ea assa

ie Dea0

10

20

G0

8030


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Anti*%rotic studies6usin$ laminectomy model7

1; Collect t"e tissue

2; De"rate in et"anol

G; Aci ecalci

8; Fait or G as

3; -lice eer 30 um


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Waterti$ht 4tudies

L; /ia et al; -urgical +eurolog


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Protein Adsorption 4tudies

2; >inse 6it" P-

/uang an ang, Polmers aance tec"nologies, 200

)ilm

1; -"a!e or 28 "ours at G7NC

/uman serum albumen an"uman plasma 4bronectin

G; Aition o soium oeclsulate (-D-*

3; Measure absorbance at 3


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Ac!no6legments

P'! Dr& 3hristine 4chmidt"Graduate 4tudents! 4arah Mayes


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3urrent Technolo$ies

Autologous grats Pericranium or temporal ascia

enograts an allograts Menengitis an Creut@elt%=acobs Disease

+atural an sntet"ic materials $ore%&e' (eP&)#*

+europatc" (polester uret"ane* Duragen (Collagen* Dura-eal (P#$%base spra* &isseel ()ibrin.trombin solutions* Preclue ( P&)#. elastomeric fuoiropolmer*

"ttp:..megaget;com.arc"ies.2003.08.uraseal;"tml

"ttp:..666;mecompare;com.etails.1


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Results


Alginate .$M/A./AControl

&"ere is no statistical iHerencebet6een control an 4lms in lie an

ea

ie Dea0

10

20

G0

8030

Documents

Natural Materials for Dural Replacement and Neuroprotection