NATIONAL FOOD NATIONAL FOOD AAND VETERINARYND VETERINARY RISK RISK ASSESSMENT INSTITUTEASSESSMENT INSTITUTE

Gediminas PridotkasDirector

31/01/2011 Vilnius

LABORATORIES OF THE SFVS

NFVRAI

Territorial Branches

Laboratories of counties SFVS

Lithuanian competent body for risk assessment in the fields of food safety and animal health

Mission – to provide scientific information and scientific and technical support in pursuance State policy in the fields of food and feed safety and veterinary and communicate on risks

Established in 2008 (originally in 1945) Branches in Kaunas, Panevėžys and Šiauliai 275 employees

NATIONAL FOOD AND VETERINARY RISK ASSESSMENT INSTITUTE

NATIONAL FOOD AND VETERINARY RISK ASSESSMENT INSTITUTE

Accreditation:

• ISO/IEC 17025 (DAkkS, Germany)

• Federal Centre of Hygiene and Epidemiology of the Federal Service for the Protection of Consumer Rights and Human Well Being (Rospotrebnadzor, Russian Federation)

• Federal Agency on Technical Regulating and Metrology (GOST-R, Russian Federation)

ILAC logo since 2007

STRUCTURE OF THE NFVRAI

THE APPROACH TO RISK ANALYSIS

EU – EFSALT - NFVRAI

Risk AssessmentHazard identification

Hazard characterizationExposure assessmentRisk characterization

EU - ECLT – SFVS

Risk ManagementLegal acts

Official controlEducation

Risk Communication

EFSANFVRAI

ECSFVS

ConsumersBusiness operators

Animal health and

welfareFood

Veterinary medicines

and biocidesFeed

RISK ASSESSMENT

RISK ASSESSMENT DEPARTMENT

SCIENTIFIC AND TECHNICAL ASSISTANCE

LEGISLATION

CONTROL

COMMUNICATION

THE APPROACH TO RISK ASSESSMENT

Analysis of Laboratory and Veterinary data

Analysis ofscientific

information

Competence of experts

Research work

Scientificcooperation

SCIENTIFIC AND TECHNICAL

ASSISSTANCE

SCIENTIFIC OPINIONS OF RISK ASSESSMENT DEPARTMENT IN 2009

Risk assessment criteria for feed operators Use of bioethanol by-products for feeding of animals Possible risk of influenza A subtype H1N1 virus to human and animal health Risk assessment of infectious laryngotraceheitis in poultry Classification of feed materials Possible negative impact of veterinary medicinal product Domitor in dogs Assessment of eradication plan of the bovine viral diarrhea Preventive vaccination against bluetongue disease Risk assessment of meat preparations Assessment of impact of ammonium to human health Laboratory analysis of imported products of animal origin Risk assessment of high, medium and low risk food establishments Risk assessment of elevated level of boron in natural mineral water Risk assessment of marketing of raw milk Risk assessment of soft ice creams Risk assessment of imported animal origin food from third countries

LABORATORY DEPARTMENT

NRLs in 36 fields covered by CRLs for food, feed and animal diseases

Laboratory tests of biological, chemical and other risk factors in food and feed

Diagnosis of animal diseases Epidemiological analysis of animal infectious diseases and

zoonoses Validation and coordination of laboratory test standards and

methods

TARGETS OF INVESTIGATIONS

STATE OFFICIAL CONTROL AND MONITORING PROGRAMS

• Monitoring of contaminants in food of plant origin

• Monitoring plan for certain substances and residues in live animals and animal origin food products

• Environment monitoring program

• Program for the control of imported foodstuffs

• Program for the control of animal infectious diseases

• Monitoring program of feed

• Plan for investigation of official samples of food and drinking water

• Monitoring of Listeria monocytogenes in food

DIAGNOSTIC OF ANIMALS INFECTIOUS DISEASES

Serology Unit

Virology Unit

Pathological Anatomy and Histology Unit

Molecular Biology and GMO Unit

Bacteriology Unit

BACTERIOLOGY UNIT

Bacteriological tests of clinical and pathological material and feedstuffs

Parasitological tests Culture identification Prophylactic analyses Antimicrobial resistance Examination of antimicrobial

substances in feedstuffs

VIROLOGY UNIT

Virological tests of domestic animals, fish and wild animals

Cell culturing, VI, VN, FAVN, IF, IFA technique

Biosafety Level 3 facilities

SEROLOGY UNIT

Monitoring programs:• Bovine, ovine and caprine brucellosis• Enzootic bovine leucosis• Classical swine fever• Swine vesicular disease• Foot and mouth disease• Aujeszky’s disease• Avian influenza• Newcastle disease

More than 50 infectious diseases can be tested for animals intended for trade, shows and competitions

Tests on milk (EBL, BRC) and sera

PATHOLOGICAL ANATOMY AND HISTOLOGY UNIT

Necropsy or postmortem examination of dead animals, birds

Gross pathology and histological analyses of delivered samples

Preparation of pathological material for virological, bacteriological and toxicological analyses

Testing of transmissible spongiform encephalopathies (TSE) by histological imunohistochemical and rapid test methods

Microscopic analyses of components of animal origin in feedstuffs

CONTROL OF FOODSTUFFS AND FEEDSTUFFS

Chemistry Unit

Molecular Biology and GMO Unit

Radiology Unit

CHEMISTRY UNIT

Chemical and physical analyses of foodstuffs, their raw materials, drinking and mineral water, beverages, feedstuffs and other

Evaluation of composition of products – proteins, fats, hydrocarbons, vitamins, humidity, ash content and other

Contaminants – mycotoxins, pesticide residues, polychlorbiphenils, benzo(a)pirene, residues of veterinary medicinal products and feedstuff additives, toxic elements

FOOD MICROBIOLOGY UNIT

Qualitative and quantitative microbiological, mycological, parasitological analyses of foodstuffs, water, beverages

Tests of irradiating foodstuffs Detection of residues of antibacterial

substances in raw milk and fresh meat Media and reagents production and

quality control

SENSORY GROUP

12 assessors were selected and trained in cooperation with Sensory Analysis Laboratory of Technological Institute in Denmark

Sensory analyses of foodstuffs, drinking water, pharmaceutical raw materials

RADIOLOGY UNIT

Radiological tests of foodstuffs and feeds, their raw materials and water

Control of imported goods from the third countries Blood biochemical analyses

MOLECULAR BIOLOGY AND GMO UNIT

Molecular qualitative and quantitative analyses of foodstuffs, feedstuffs, plants and their seeds

Molecular microbiological, virological analyses to determine rabies, classical swine fever, avian influenza, bovine viral diarrhoea and other diseases

Polymerase chain reaction (PCR), AT-PCR, real time PCR, genotyping and sequencing analyses

TERRITORIAL BRANCHES

Bacteriology analyses subdivision – bacteriological, parasitological and mycological tests of clinical and pathological material of animals

Serology analyses subdivision – infectious diseases in domestic animals and wild fauna (analysis from milk and blood)

Microbiology analyses subdivision – quantitative and qualitative microbiological, mycological, parasitological analyses of foodstuffs

Chemistry analyses subdivision – chemical and physical analyses of foodstuffs, drinking and mineral water, beverages, feedstuffs; blood biochemical analyses

Pathological anatomy and histology subdivision – sections

of dead animals, gross pathology and histological analyses of delivered samples

Virology analyses subdivision – analyses of rabies of animals

LABORATORY INVESTIGATIONS IN 2004-2010

824982

637702

795482845875

944035

670144

1023334

0

200000

400000

600000

800000

1000000

1200000

2004 2005 2006 2007 2008 2009 2010

NUMBER OF LABORATORY INVESTIGATIONS IN 2010

43% (352390)

57% (472592)

Commercial tests Official control programs

NUMBER OF LABORATORY INVESTIGATIONS AND NON-COMPLIANT CASES IN 2007-2010

845875 795482

637702

824982

18246 19227 12035 174060

100000

200000

300000

400000

500000

600000

700000

800000

900000

2007 2008 2009 2010

Laboratoryinvestigations

Non-compliantcases

NUMBER OF NON-COMPLIANT FOOD AND FEED SAMPLES IN 2010

Indicators Number of samplesResidues of antimicrobial substances 23

Residues of veterinary medicinal products 11

Chloramphenicol 27Residues of pesticides 23Heavy metals 28Salmonella spp. 73Legionella 5Listeria monocytogenes 38

Iron (drinking and mineral water) 226Parasites in food products 173Dioxins, furanes and PCBs 2Organoleptic parameters 42GMO 14

UNSAFE FOODSTUFFS AND FEEDINGSTUFFS IN 2009

Product Country of origin Amount, t Indicator

Frozen pike China 14 Nematodes

Rice Pakistan 25 Mycotoxins

Frozen pork neck meat United Kingdom 5,6 Organoleptic parameters

Food supplements Canada 0,64 >5% GMO

Canned herring fillet Belarus 2,05 Listeria monocytogenesSunflower oil Ukraine 59,12 Benzopyrene

Grapes Turkey 19 Metoxychlore

Nuts butter The Netherlands 1,17 Organoleptic parameters

Apples Poland 0,1 Chlorbuphame

Waffle Turkey 0,74 >5% GMO

Frozen fish fillet Kazachstan 16,2 Trematodes

Pistachio Iran 12 Aflatoxins

Frozen poultry meat Poland 10,6 Salmonella spp.

Canned fish Russia 14,1 Nematodes

Chicken leg meat Poland 3,5 Listeria monocytogenes

Pet food USA 19,4 >5% GMO

Chocolate sweets Turkey 7,2 >5% GMO

Orange Turkey 19,5 Chlorpyrifose

Pomegranate Israel 1,6 λ-cihalotrine

Feedingstuffs The Netherlands 300 >5% GMO

UNSAFE FOODSTUFFS AND FEEDINGSTUFFS IN 2010

Product Country of origin Amount, t Indicator

Eggs Lithuania 360 pcs. Salmonella spp.

Rice Brazil 0,002 Organoleptic parameters

Orange Egypt 59,67 Phenitrotione

Orange Egypt 20,28 Daiazinone

Sweets Turkey 0,69 >5% GMO

Pig muscle Lithuania 9,77 Spectynomycine, lincomycine

Frozen fish fillet Russia 18 Nematodes

Herring from Baltic Sea Lithuania 0,3 Dioxins

Poultry meat Poland 1005 Salmonella spp.

Feedingstuffs The Netherlands 2005,5 >5% GMO

Golden raisins Iran 2380 Carbendazim and benomyl

Sausages "without preservatives"

Lithuania 725,61 Preservatives

Drinking water Lithuania 360 pcs. Pseudomon

Green grapes India 15840 Chlormequat

Black tea India 200 Monocrotophos

Pomegranate Egypt 100 Methomyl and thiodicarb

Pasta with chicken aroma China 1760 Aluminum

Hot smoked sausages Lithuania 50 Listeria Monocytogenes

Number of consignments of unsafe imported foodstuffs 2010

2

4

2

1

7 7

1

3

1

10

1

7

9

3

1

9

8

1 1 1

2

0

2

4

6

8

10

12

957

594

17 14 1399 89

198 206

1179

49 380

200

400

600

800

1000

1200

1400B

ovin

e an

imal

s

Pig

s

Hor

ses

Sh

eep

an

d g

oats

Rab

bit

s

Far

med

wil

d a

nim

als

Wil

d a

nim

als

Pou

ltry

Egg

s

Mil

k

Fis

h

Hon

ey

Animals and products of animal origin

Nu

mb

er o

f sa

mp

les

Number of samples tested for residues in live animals and products of animal origin in 2010 in Lithuania

0,43

0,5

0,2

0,3

0

0,1

0,2

0,3

0,4

0,5

0,6

0,7

2010, animals (N = 2030; n = 6)

2010, animal origin products (N = 1423; n = 3)

2009, animals (N = 1800; n = 9)

2009, animal origin products (N = 1632; n = 7)

Animals and animal origin products

pre

va

len

ce o

f re

sid

ues

(%

)Prevalence of residues in live animals and products of

animal origin in 2009 – 2010 in Lithuania

3,2

2,12

0

0,5

1

1,5

2

2,5

3

3,5

4

4,5

2010(N = 377; n = 12)

2009 (N = 189; n = 4)

Num

ber

of u

ncon

form

itie

s (%

)Number of non-conformities of feed safety

(monitoring) and labeling in 2009 – 2010 in Lithuania

Investigation of imported consignments in 2009 – 2010 in Lithuania

00

7

0

10

4,1

18,6

5,6

0

2

4

6

8

10

12

14

16

18

20

2010 (NF; P; N =160; n = 9)

2009 (NF; P; N =102; n = 19)

2010 (PF; P; N =74; n = 3)

2009 (PF; P; N =70; n = 7)

2010 (PF; L; N =108; n = 0)

2009 (PF; L; N =215; n = 15)

2010 (PF; S; N =119; n = 0)

2009 (PF; S; N =33; n = 0)

Samples

Nu

mb

er o

f p

osi

tiv

e sa

mp

les

(%)

NF – non processed fish; PF – processed fish; P – parasites; L – Listeria; S – Salmonella.

NUMBER OF POSITIVE CASES OF ANIMAL CONTAGIOUS DISEASES IN 2010

*- Antibodies after vaccination*- Antibodies after vaccination

Disease Number of positive casesRabies 33Efficiency of oral vaccination of wild animals against rabies

71,1 % (tetracycline marker) /59,6 % (Ab in blood)

Salmonella spp. infections 17Colibacteriosis 131Streptococcal infections 99Staphylococcal infections 195Anaerobic infections 34Pseudomonosis 51Mastitis 1620Enzootic bovine leukosis 465Leptospirosis 299*Infectious bovine rhinotracheitis (Ab) 333Bovine viral diarrhoea (Ab) 189Bovine respiratory syncytial viral infection (Ab) 212Bovine parainfluenzae 3 (Ab) 182Mycoplasmosis (Ab) 4007Aujeszky’s disease (wild boars, Ab) 13Parvoviral infection (Ab) 419*Newcastle disease (Ab) 199*

5,6

2,8

0

1

2

3

4

5

6

7

8

9

2009 (N = 1143; n = 64) 2010 (N = 1166; n = 33)

Pre

vela

nce

(%

)Prevalence of rabies in 2009 – 2010 in Lithuania

74,4

71,1

66

68

70

72

74

76

78

2009(N = 2459; n = 1830)

2010(N = 4448; n = 3161)

Eff

icac

y (%

)Efficacy of intake of baits with vaccine by wild animals

in 2009 – 2010 in Lithuania

49,7

59,6

0

10

20

30

40

50

60

70

2009 (N = 290; n = 144) 2010 (N = 1164; n = 694)

Eff

ecti

vene

ss (

%)

Seroconversion of wild animals vaccinated against rabies in 2009 – 2010 in Lithuania

89,892,5

98,2 99,3

80

85

90

95

100

105

Dogs (2008; N=548; n =492)

Dogs (2009; N =624; n = 577)

Cats (2008; N =109; n = 107)

Cats (2009; N =143; n = 142)

Pets

Eff

ecti

ven

ess

(%)

Effectiveness of vaccination against rabies of pets in 2008 – 2009 in Lithuania

4,6

3

6,2

3,9

0

1

2

3

4

5

6

7

2009 (DP; N = 230; n = 9)

2010 (DP; N = 433; n = 27)

2009 (WB; N = 3181; n = 95)

2010(WB; N = 2149; n = 98)

Animals

Pre

vale

nce

(%)

Prevalence of Trichinella in domestic pigs (DP) and wild boars (WB) in 2009 – 2010 in Lithuania

0,5

0,7

00,2

1

3,3

0

0,3

0

0,4

0,8

1,2

1,6

2

2,4

2,8

3,2

3,6

Breding (N 527;n 0)

At the hatchery(N 479; n 1)

Epheneral (N296; n 2)

Pullets (N 285; n3)

Lying hens (N333; n 11)

Eggs (N 173; n 0) Broilers in flocks(N 707; n 2)

Broiler carcasses(N 420; n 2)

category of poultry

prev

alen

ce, %

Investigation of prevalence of Salmonella in poultry flocks in 2009 – 2010 in Lithuania

243

84112

1936 2168 20041577

77168

2060

10000

20000

30000

40000

50000

60000

70000

80000

90000

Slaughtered Emergencyslaughtered

With clinical signs Dead

Risk category

Num

ber

of t

este

d bo

vine

ani

mal

s

2009

2010

Number of bovine animals tested for BSE in 2009 – 2010 in Lithuania

Thank you for your attention!