NanobioprocessingNanobioprocessing Larry P. Walker, Ph.D. Professor, Department of Biological and...
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Nanobioprocessing Nanobioprocessing Larry P. Walker, Ph.D. Larry P. Walker, Ph.D. Professor, Department of Professor, Department of Biological and Environmental Biological and Environmental Engineering Engineering Cornell University Cornell University
NanobioprocessingNanobioprocessing Larry P. Walker, Ph.D. Professor, Department of Biological and Environmental Engineering Cornell University
NanobioprocessingNanobioprocessing Larry P. Walker, Ph.D.
Professor, Department of Biological and Environmental Engineering
Cornell University
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Agricultural and Environmental Bioprocess Engineering Research
Molecular mechanisms of polysaccharide degrading enzymes, Solid
state microbial processes and molecular ecology, Mechanisms and
kinetics of metal uptake and sequestration in plants, Molecular
filtration and analysis of biomolecules
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Nanobiotechnology Center (NBTC) Cornell University The
Nanobiotechnology Center (NBTC) was established in January 2000 as
a Science & Technology Center, with core funding from the
National Science Foundation. Nanobiotechnology is an emerging area
of scientific and technological opportunity that integrates
nano/microfabrication and biosystems to the benefit of both.
www.nbtc.cornell.edu
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Nanobiotechnology is beginning to generate substantial new
insights into how biological systems function, and likewise,
nanobiotechnology will lead to the design of entirely new classes
of micro- and nanofabricated devices and systems. Nanobiotechnology
Center (NBTC) Cornell University
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Nanobiotechnology Center (NBTC) Cornell University Cornell
University Clark Atlanta University Howard University Princeton
University Oregon Health Sciences University Wadsworth Center New
York Department of Public Health
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Nanobiotechnology Center (NBTC) Cornell University Cellular
Devices (David Lawrence, Wadsworth Center) studies of engineered
devices for separating and analyzing individual cells. Nanoscale
Cell Biology (Manfred Lindau, Cornell University) - micro- and
nanofabricated tools to understand and utilize cellular responses
on the nanoscale. Biomolecular Dynamics (Michael Koonce, Wadsworth
Center) exploration and utilization of molecular motion and
mechanical properties
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Nanobiotechnology Center (NBTC) Cornell University Cell-Surface
Interactions (Harvey Hoch, Cornell University) Investigating the
response and control of cellular responses on structured surfaces
Biomolecular Devices and Analysis (Larry Walker, Cornell
University) - investigation new approaches to high speed and highly
sensitivity analysis of biomolecules. Nanoscale Biomaterials (Dotse
Sogah, Cornell University) study of materials engineered at the
nanoscale for new properties that can be exploited in
nanobiotechnolgy.
Slide 8
Major research and development activities in the life sciences
has generated the need for materials, methods, and devices for
sorting, separating, and analyzing proteins, DNA and other
biomolecules. Biomolecular Devices and Analysis
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Membranes and Packings Semipermeable Membranes and Equilibrium
Dialysis Michael Spencer, Lori Lepak, Jim Turner, Michele Caggana
Fabrication of Integratable Semipermeable Membranes Jim Turner,
Peter Russo, David Martin, David Lawrence, Michele Caggana, Bill
Shain, Michael Isaacson, and Michael Spencer High Resolution
Protein Purification using Block Copolymer Derived Mesoporous
Silica-type Materials Uli Wiesner, Phong Du, Caroline Corner, &
Larry Walker Porous Block Copolymers For Microfluidic Separation
Chris Ober &Mingqi Li
Slide 10
Micro and Nanofluidic devices Micro and Nanofluidics for
Molecular Separation and Detection Harold Craighead, John Henion,
David Czaplewski, Yanou Yang & Jun Kameoka Multidimensional
Microscale Separations of Cerebrospinal Fluid Proteins Kelvin Lee,
Michael Shuler, Jim Engstrom, Bob Austin, Chen Li & Jinpian
Diao Nanostructures for Enzyme Transport and Accessibility
Assessment Larry Walker, Harold Craighead, Jennifer Guisado &
Tina Jeoh
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Biomolecular Devices and Analysis Some Common Activities:
Lithography Chemical synthesis Spinning of molecules Assessing
morphology Assessing monodispersity Assessing transport mechanisms
Measurement & Analysis
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Biomolecular Devices and Analysis Biocompatibility, Working
with small volumes: pico- to micro-liters, Sieving and sorting at
the scale of 5, 10, 15 and 50 nm, Monodispersity tight control over
pore size distribution and shape
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Example: Membrane Technology Si Si3N4 Spun Organic membrane
Spin Coating of Cellulose And Collagen onto silicon surfaces to
create porous membranes
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Fabrication of Integratable Semipermeable Membranes M. Spencer
1 layer collagen:Methyl orange (MW 327) diffuses through in 10 min
4 layers collagen:No methyl orange diffuses in 10 hours (up to 48
hours!) 400 nm thick Pores 0-100nm Collagen Dyes of 270 390 Da
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Block Co-polymers Uli Wiesner & Chris Ober 12 hrs 400V/cm
(b) (c) ChemicalSynthesis PhotoLithography excellent
biocompatibility of silica easily accessible pore sizes from 8-50
nm narrow pore size distributions
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Nanostructures for Enzyme Transport and Accessibility
1.Studying cellulase binding and catalysis as a function of
molecular diameter. 2.Study synergism in cellulase/ cellulose
reaction systems.
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Channel design & fabrication Make inlets and outlets
Glass-Glass bonding Attach peripheral components Flow chart of the
device fabrication process New 2 stage bonding approach developed
to permit alignment and bonding. GMA-co-TRIM polymer Double-T
Structure Channel with affinity separation bed 5 m Multidimensional
Microscale Separations Proteins K. Lee
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Micro and Nanofluidics for Molecular Separation and Detection
H. Craighead Bottom Plastic Chip Embossed Top Plastic Chip
Reservoir hole Embossed Fluidic Channel Emitter filmBonding
Electrospray Ionization
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(a) (b) Triangle Nozzle Taylor cone Liquid jet Plume 50 m
Berberine:FW=336 (a) (b) Application to Mass Spectrometry
Analysis
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NBTC At the Interface between Biology and Engineering
Nanobiotechnology Advanced Bioreactors Advanced Energy Systems
Controlled Environment Agriculture Systems Genomics Proteomics
Metabolic Engineering Protein Engineering