Nahuman Jud Mygad Finaaalll

7/25/2019 Nahuman Jud Mygad Finaaalll

1/32

PANCREATICAMYLASETest 1


2/32

A.) IN THIS PROCEDURE, IDENTIFY THEENZYME, THE SUBSTRATE, AND THEMANIPULATED VARIABLE.

Enzye! AMYLASE

S"#st$%te! STARCH

M%n&'"(%te V%$&%#(e! TYPE OF ENZYME


3/32

B.) *HAT IS THE PRINCIPLE BEHIND THUSE OF THE BENEDICT+S AND BARFOE

TESTS Bene&-ts+s test &t &s % test "se t/ ete$&ne t0e '$esen-e / s&

s"2%$.

B%$/e+s test test "se /$ t0e ete-t&/n / /n/s%--0%$&es.


4/32

REACTION INVOLVED


5/32

REACTION INVOLVED


6/32

B%$/e+s Re%2ent

RCHO 3 4C"433 4H4O 5 RCOOH 3 C"4O6

7H3


7/32

SUBSTANCES

Bene&-t+s Re%2ent

B$&-8$e '$e-&'&t%te

9$een t/ ye((/: ; /$%n2e s/("t&/n

B("e s/("t&/n

B%$/e+s Re%2ent

B$&-8$e '$e-&'&t%te

B("e s/("t&/n


8/32

S/&" -%$#/n%te! It %-ts %s % -%t%(y/$ $e(e%s&n2 enzyes $/ t0e'%n-$e%t&n s/ &t -%n #$e%8 /:n -%$#(&'&s, /$ '$/te&n =%&n(y st%$-0, t0/"


9/32

*HAT IS THE PURPOSE OF >.?@ SODIUCARBONATE SOLUTION IN THEEPERIMENT

It %-ts %s % -%t%(yst /$$e(e%s&n2 enzyes $/ t0e'%n-$e%t&n s/ &t -%n #$e%8/:n -%$#s, (&'&s, /$ '$/te&=%&n(y st%$-0, t0/"20).


10/32

HO* DOES SALIVARY AMYLASE COMPA*ITH PANCREATIC AMYLASE IN ITS ABIL

TO DI9EST CARBOHYDRATES

SALIVARY AMYLASE

C0e:&n2 #$e%8s // &nt/ s%(( /(e-"(es t0%t -/#&ne :&t0 s%(&se-$ete #y t0e s%(&%$y 2(%ns &n t0e /"t0. A(/n2 :&t0 "-&n %n#"e$s, s%(&% -/nt%&ns t0e enzye s%(&%$y %y(%se, :0&-0 %-ts /

st%$-0 &n // %n #$e%8s &t /:n t/ %(t/se. S%(&%$y %y(%se -//$ t0e s0/$t "$%t&/n t0%t t0e -%$#/0y$%tes %$e &n t0e /"t0, %tt0e &t"$e / t0e '%$t&%((y &2este -%$#/0y$%tes t$%e(s /:n tes/'0%2"s &nt/ t0e st/%-0. D"e t/ t0e &n0&t&/n / s%(&%$y %y%-t&&ty #y t0e %-&&- 2%st$&- "&-es, &2est&/n / -%$#/0y$%tes //--"$ &n t0e st/%-0.


11/32

PANCREATIC AMYLASE

As t0e -/#&n%t&/n / 2%st$&- "&-es %n '%$t&%(&2este // ente$s t0e s%(( &ntest&ne, t0e '%se-$etes '%n-$e%t&- "&-es, :0&-0 -/nt%&n t0e en'%n-$e%t&- %y(%se. T0&s enzye %-ts /n t0e$e%&n&n2 '/(ys%--0%$&es %n #$e%8s t0e &nt

&s%--0%$&e "n&ts / %(t/se. In t0e n%( ste' /-/'(e -%$#/0y$%te &2est&/n, t0e enzye '$esent &n t0e (&n&n2 / t0e s%(( &ntest&ne #$e%8%(t/se &nt/ t:/ "n&ts / 2("-/se. 9("-/se &s t0%#s/$#e %n ente$s t0e #(//st$e%.


12/32

PANCREATIC AMYLASE

SALIVARY AMYLASE vs. PANCREATIC AMY

SALIVARY AMYLASE &s '$/"-e &n t0e s2(%ns %n &2est&/n / s&'(e -%$#/0y$%tes

&n t0e /"t0.

PANCREATIC AMYLASE %$e enzyes '$/"-e'%n-$e%s, %-t&n2 /$e /n -/'(e -%$#/0y$%


13/32

PANCREATICPROTEASESTest 4


14/32

FUNCTION OF EVERYCOMPONENT USED IN TH

TESTC"SO7 $e"-es t0e '$/'e$ty / s&'(e -%$#/0yN%OH$%&ses t0e 'H / t0e s/("t&/n t/ %(8%(&ne

HC( en%t"$es '$/te&n st$"-t"$eG %-t&%tes'e's&n/2en t/ 'e's&n

Pe's&n 0y$/(yzes '$/te&ns t/ s%((e$ '/(y'e't%n s/e $ee %&n/ %-&s

P%n-$e%t&n % &t"$e / enzye $/ t0e '%n-"&-e


15/32


16/32


17/32


18/32

PURPOSE OF N NAOH ANN HCLT0e '$/te%se enzyes '$/"-e &n t0st/%-0 :/$8 #est &n %-&&- -/n&t&/n

T0&s enzye &s -%((e 'e's&n. T0e st/'$/"-es 0y$/-0(/$&- %-& s/ t0e enz

'$/"-e 0e$e "st #e %%'te t/ :#est &n t0ese -/n&t&/ns. T0e /'t&"/$ st/%-0 'e's&n &s %''$/. 'H4


19/32

P%n-$e%t&n :/$8s #est "ne$ #%s&-en&$/nent. It 0%s t0e /'t&" 'H t/ J..

T0e 'H &n t0e st/%-0 &s e$y %-&&-:0&-0 :/"( est$/y '%n-$e%t&n "n(es'e-&-%((y #"e$e.


20/32

PEPSIN %n enzye t0%t #$e%8s /:n '$/te&ns &nt/ s'e't&es =t0%t &s, % '$/te%se). It &s '$/"-e &n st/%-0 %n &s /ne / t0e %&n &2est&e enzy

t0e &2est&e systes / 0"%ns %n %ny /t0%n&%(s, :0e$e &t 0e('s &2est t0e '$/te&ns &n /


21/32

PANCREATIN

P%n-$e%t&n -/nt%&ns t0e '%n-$e%t&- enzyt$y's&n, %y(%se %n (&'%se. A s&&(%$ &tenzyes &s s/( %s '%n-$e(&'%se, :0&-0 -//$e %-t&e (&'%se enzye t0%n '%n-$e%t&t$y's&n /"n &n '%n-$e%t&n :/$8s t/ 0y$/'$/te&ns &nt/ /(&2/'e't&esG %y(%se 0y$/st%$-0es &nt/ /(&2/s%--0%$&es %n t0e&s%--0%$&e %(t/seG %n (&'%se 0y$/(yzt$&2(y-e$&es &nt/ %tty %-&s %n 2(y-e$/(s
https://en.wikipedia.org/wiki/Trypsinhttps://en.wikipedia.org/wiki/Pancrelipasehttps://en.wikipedia.org/wiki/Pancrelipasehttps://en.wikipedia.org/wiki/Trypsin


22/32

P%n-$e%t&n &s %n ee-t&e enzye s"''(e/$ $e'(%-&n2 &ss&n2 '%n-$e%t&- enzyes%&s &n t0e &2est&/n / //s &n -%ses /'%n-$e%t&- &ns"K-&en-y.


23/32

PANCREATIC JUICE

% (&"& se-$ete #y t0e '%n-$e%s, :0&-0 -/nt%$&ety / enzyes, &n-("&n2 t$y's&n/2en,-0y/t$y's&n/2en, e(%st%se,-%$#/y'e't&%se, '%n-$e%t&- (&'%se, n"-(e%ses%y(%se.


24/32

*HERE ARE THESESYNTHESIZED AND *HATTRI99ERS THEIR RELEAS


25/32

PEPSIN Pe's&n &s /st %-t&e &n %-&&- en&$/nents #et:een

%n 74 C. A--/$&n2(y, &ts '$&%$y s&te / synt0es&s %%-t&&ty &s &n t0e st/%-0 ='H 1.? t/ 4). Pe's&n :&(( &2t/ 4>@ / &n2este %&e #/ns #y -(e%&n2 '$ee$en%te$ t0e Nte$&n%( / %$/%t&- %&n/ %-&s s"-0 %s'0eny(%(%n&ne, t$y't/'0%n, %n ty$/s&ne. Pe's&n e0'$ee$ent&%( -(e%%2e /$ 0y$/'0/#&-, '$ee$%#(y %$/

$es&"es &n P1 %n P1 '/s&t&/ns. Pe's&ns '$/enzye, pepsinogen, &s $e(e%se #y t0e-0&e -e((s&n t0e st/%-0 :%((, %n "'/n &&n2 :&t0 0y$/-0(/$&- %-&/ t0e 2%st$&- "&-e, 'e's&n/2en %-te-/e 'e's&n. Pe's&n &s %n %s'%$t&- '$/te%se, "s&n2 -%t%(yt&- %s'%$t%te &n &ts %-t&e s&te.
https://en.wikipedia.org/wiki/Phenylalaninehttps://en.wikipedia.org/wiki/Tryptophanhttps://en.wikipedia.org/wiki/Tyrosinehttps://en.wikipedia.org/wiki/Zymogenhttps://en.wikipedia.org/wiki/Gastric_chief_cellhttps://en.wikipedia.org/wiki/Hydrochloric_acidhttps://en.wikipedia.org/wiki/Gastric_acidhttps://en.wikipedia.org/wiki/Aspartic_proteasehttps://en.wikipedia.org/wiki/Active_sitehttps://en.wikipedia.org/wiki/Active_sitehttps://en.wikipedia.org/wiki/Aspartic_proteasehttps://en.wikipedia.org/wiki/Gastric_acidhttps://en.wikipedia.org/wiki/Hydrochloric_acidhttps://en.wikipedia.org/wiki/Gastric_chief_cellhttps://en.wikipedia.org/wiki/Zymogenhttps://en.wikipedia.org/wiki/Tyrosinehttps://en.wikipedia.org/wiki/Tryptophanhttps://en.wikipedia.org/wiki/Phenylalanine


26/32

PANCREATIN

P$/"-e #y t0e e/-$&ne -e((st0e '%n-$e%s


27/32

PANCREATIC UICE

P%n-$e%t&- "&-e se-$et&/n &s $e2"(%te #y 0/$/nesse-$et&n%n -0/(e-yst/8&n&n, :&s '$/"-e #y t0e :%((s / t0e "/en""'/n ete-t&/n / %-& //, '$/te&ns, %t&t%&ns.
https://en.wikipedia.org/wiki/Hormoneshttps://en.wikipedia.org/wiki/Secretinhttps://en.wikipedia.org/wiki/Cholecystokininhttps://en.wikipedia.org/wiki/Duodenumhttps://en.wikipedia.org/wiki/Duodenumhttps://en.wikipedia.org/wiki/Cholecystokininhttps://en.wikipedia.org/wiki/Secretinhttps://en.wikipedia.org/wiki/Hormones


28/32


29/32

C%$#/y'e't&%se

P$/te%se enzye t0%t -(e%es % 'e'#/n %t t0e -%$#/y( te$&n%( en.

N"-(e%seEnzye -%'%#(e / -(e%&n2 t0e

'0/s'0/&este$ #/ns #et:een t0en"-(e/t&e s"#"n&ts / n"-(e&- %-&s


30/32

L&'%se

Use t/ #$e%8 /:n t$&2(y-e$&es &n$ee %tty %-&s %n /n/2(y-e$&es

C0y/t$y's&n/2enUse t/ #$e%8 'e't&e %&e #/ns

:0e$e t0e -%$#/y( s&e / t0e %&e#/n &s (%$2e 0y$/'0/#&- %&n/ %-&=ty$/s&ne, t$y't/'0%n %n '0eny(%(%n


31/32

Ay(%se#$e%8s /:n st%$-0 &nt/et$/se %n %(t/se


32/32

HO* DOES PEPSIN COMPARE *ITH PANCREATICPROTEASES IN ITS ABILITY TO DI9EST PROTEINS

Pe's&n &s %n enzye t0%t #$e%8s /:n '$/te&ns &ns%((e$ '%$t&-(es. In &2est&/n '$/-ess, t0ese enze%-0 / :0&-0 &s s'e-&%(&ze &n see$&n2 (&n8s #et:'%$t&-"(%$ %&n/ %-&s, -/((%#/$%te t/ #$e%8 /:n&et%$y '$/te&ns &nt/ t0e&$ -/'/nents :0&-0 -%n$e%&(y %#s/$#e #y t0e s%(( &ntest&ne. *0&(e t0e'%n-$e%t&- '$/te%se #$e%8s /:n /st / t0e ty''$/te&ns. It se-$ete &n t0e ("en %n "st #e-/ne$te &nt/ t0e&$ %-t&e /$ t/ &2est '$/te&n

Documents

Nahuman Jud Mygad Finaaalll