Multicolor immunohistochemistry lets you visualize biology within your precious samples by allowing simultaneous phenotyping and functional assessment of multiple cell types. As a result, multiplex IHC images lead to a comprehensive understanding of complex cellular interactions that is not accessible by other methods.

Opal™ Multicolor IHC Kits make multiplex methods accessible to anyone who works with standard immunohistochemistry. Antibodies for simultaneous IHC may be selected based on performance, rather than species. The method is compatible with the standard research IHC workflow in your lab. Opal kits are optimized for reliable spectral unmixing and simultaneous measurement of 3 to 6 IHC targets, plus a nuclear stain.

• Measure 3 to 6 tissue biomarkers simultaneously• Use the best primary antibodies, regardless of species—with no crosstalk• Retain spatial cellular context that is lost when using other methods• Get more information from scarce samples

Phenotype and Visualize with Opal Kits

MULTIPLEX TISSUE BIOMARKERSIN CONTEXT

Above Image: PD-L1 (rabbit)---Yellow; CD8 (mouse)---Red; CD4 (mouse)---Green; CD20 (mouse)---Orange; Cytokeratin AE1/AE3 (mouse)---Cyan; Nuclei (DAPI)---Blue. Staining with Opal method and TSA Plus Fluorescent reagents, imaged on the Vectra. Image Source: PerkinElmer Tissue Applications Team

4-color multiplex detection - melanoma tissue stained with the Opal™ 4-Color Fluorescent IHC Kit, imaged on the Vectra quantitative pathology workstation. FOXP3-green. PDL-1-red. CD8-yellow. DAPI-blue. Image courtesy of the Fox lab, Earle A. Chiles Research Institute

What can you achieve with Opal?

PerkinElmer’s applications team has demonstrated a range of multiplex biomarker panels in FFPE tissue for research.

• Cancer immunology checkpoint assay: PD-L1, FOXP3, CD8,CD34, cytokeratin

• Tumor infiltrating lymphocytes: CD4, CD8, CD20, cytokeratin• Breast cancer: ER, PR, HER2, Ki-67, cytokeratin

For the latest Opal assays and images, visit www.perkinelmer.com/opal.

For more information please contact your local sales representative or visit www.perkinelmer.com/Opal

For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs

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PerkinElmer, Inc. 940 Winter Street Waltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602www.perkinelmer.com

How Does Opal Work?

Opal follows the standard IHC workflow using your unlabeled primary antibodies, followed by the addition of anti-species-HRP conjugate and detection substrate. Opal fluorescent detection substrates bind covalently near the epitope, allowing subsequent removal of antibodies to clear the tissue for detection of the next target. The signal remains intact after antibody removal. A 4-color Opal staining protocol typically takes one day to complete and all targets are imaged at the same time.

Quantitative Pathology Imaging Systems – A Complete Solution

Mantra™ and Vectra® imaging systems use multispectral unmixing to provide quantitative results for each marker in a multicolor IHC slide, making them ideal for imaging and analysis of Opal samples. When combined with InForm® analysis software, this novel solution enables automated cellular phenotyping and tissue segmentation for virtually unlimited investigation of the biology expressed in your samples.

Mantra Quantitative Pathology Workstation

Opal, Vectra and Mantra products are for research use only; and not intended for use in diagnostic procedures.

Melanoma tissue stained using PerkinElmer Opal Multiplex Tissue Reagents, imaged with PerkinElmer Vectra Automated Quantitative Pathology Imaging System, and analyzed with PerkinElmer inForm Advanced Image Analysis Software. Left: Simulated brightfield IHC rendition, Right: Spectrally unmixed composite image. Tissue samples courtesy Bernard A. Fox, Ph.D.,

Oregon Health & Science University, Providence Cancer Center.

All kits include Opal fluorophores, DAPI and AR6 buffer. *Opal 5-, 6-, and 7-color kits require multispectral imaging. See options below.

Ordering Information

Opal 4-color IHC Kit50 slides NEL794001KT

250 slides NEL794B001KT

Opal 5-color IHC Kit* 50 slides NEL795001KT

250 slides NEL795B001KT

Opal 6-color IHC Kit*50 slides NEL796001KT

250 slides NEL796B001KT

Opal 7-color IHC Kit*50 slides NEL797001KT

250 slides NEL797B001KT