Weighing and Analysis in the Laboratory

Multiparameter Titration System Guarantees Product Quality

One sample – several analysesLiquid samples are perfectly suited for determining various parameters. Qual-ity control labs often need to determine pH, color and perform titrimetric analyses such as acid/base, redox or surfactant ti-trations. A simple example involves saline solutions, where the pH value and color are important. Both are determined with direct measurements.

The multi-parameter measuring systemThanks to the METTLER TOLEDO multi-parameter measuring system, all analy-ses can be performed in a fully automated system, managing comprehensive calcu-lations to a single record, which can then be printed or saved to a PC. The operator simply places a sample tube containing

20 mL sample onto the rack of the sample changer and begins the measurements. The system measures the pH value direct-ly in the sample tube and then fills the flow through cell of the colorimeter and triggers the measurement. The new Excellence titrator is an inte-gral part of this system. It controls the Rondo 60 sample changer, which can pro-cess up to 60 samples. The titrator also automatically activates pumps that clean the system after each sample. It sends the signal to the colorimeter to start the measurements and waits for the results, which are calculated and saved in the ti-trator. LabX titration PC software gathers all data and saves it to the database where it can be recalled at a later date. Regula-tory requirements are easily fulfilled us-

3News

Cosmetics, Fragrances, Food Ingredients

Analytical solutions in the laboratory

The quality of ophthalmic saline solutions is mo nitored and safe guard-

ed by a multitude of analyses. The METTLER TOLEDO multi-parameter

measuring system automatically performs these analyses, facilitating

quality monitoring and increasing the reliability of the production

process.

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METTLER TOLEDO Cosmetics, Fragrances, Food Ingredients 3

Publisher Mettler-Toledo AG Laboratory Division Im Langacher CH-8606 Greifensee, Switzerland

Production Segment Marketing LABGlobal MarCom Switzerland

Subject to technical changes. © Mettler-Toledo AG 02/11Printed in Switzerland.

ing LabX titration PC software, as it fully supports compliance with the FDA 21 CFR part 11 regulation offering audit trail, user management, password protection with password history and much more.

Numerous samples just a click awayAll the user has to do is take the sample and start the method using One Click®.

Best Practice

GTP – Good Titration Practice™

GTP™ covers the entire lifecycle of your investment. Dependable titration starts long before daily routines in the laboratory: A requirements-based selection of the titration system as well as professional installation and training form the basis of dependable and risk-free titration.METTLER TOLEDO’s many years of expertise and experience are available in comprehensive literature and trained sales consultants.

www.mt.com/gtp

For further information, go to:

GTP™

Good Titration Practice™

Each user has their own shortcut keys, enabling direct access to their tasks. Other devices, such as density meters, refractometers, turbidity sensors or colori meters are monitored by the titrator and their results saved. With the new ver-sion 3.1 Excellence titrators, METTLER TOLEDO is leading where others follow.

www.mt.com/one-click-titration

3METTLER TOLEDO Cosmetics, Fragrances, Food Ingredients 3

HP DSC1

Fish oil oxidizationFish oil is known to provide many health benefits such as protection against heart disease. However, as it oxidizes rapidly, it not only affects the taste of the supple-mented product, but the oxidized fish oil can also contain toxic chemical elements. Therefore, detailed knowledge of the oxi-dation stability of fish oil provides valu-able information for research and devel-opment, quality control and the storage conditions for the supplemented food products.

Investigating the oxidation stability by HP DSCFish oil should be investigated for stability using oxidation induction time (OIT). For

Stability of Alginates and Fish Oil

Many food manufacturers have taken advantage of healthy attributes

by the inclusion of fish oil in conventional food products. However, a

common problem associated with the use of fish oil is the rapidity with

which the oil oxidizes and becomes rancid. High pressure differential

scanning calorimetry is an excellent technique for investigating the

oxidation stability of fish oil and alginates.

OIT the onset of exothermic oxidation is evaluated. A long OIT indicates the good oxidation stability of a substance. The following example illustrates how the METTLER TOLEDO High Pressure Differ-ential Scanning Calorimetry (HP DSC 1), equipped with an FRS 5 sensor, was used to investigate the oxidation stability of a pure fish oil sample and a processed fish oil/alginate sample. The samples were heated from 30 °C to 100 °C at a heating rate of 100 °C/min in an oxygen atmo-sphere with 35 bar pressure. At 100 °C the measurement continued isothermally.

The HP DSC curves in figure 1 show that the OIT of the processed fish oil/alginate

sample is significantly shorter compared to that of the pure fish oil sample. This suggests that the pure fish oil has a high-er oxidation stability. The curves also show very good reproducibility.

High pressure differential scanning calo-rimetry is an excellent technique for in-vestigating the oxidation stability of fish oil and alginates. The METTLER TOLEDO HP DSC 1 is a valuable instrument for research and development and quality control. The wide temperature and pres-sure range enable reactions to be acceler-ated. This process results in shorter anal-ysis times, which can lead to reducing the time required for stability testing.

www.mt.com/dsc

For further information, go to:Figure 1: OIT of pure fish oil and fish oil/alginate by HP DSC.(Source: Thermal Analysis Report 9976)

4 METTLER TOLEDO Cosmetics, Fragrances, Food Ingredients 3

GWP® Solutions forBetter Weighing Results and Processes

In an effort to optimize their processes, TheraSkin has adopted the

METTLER TOLEDO Good Weighing Practice™ (GWP®) guideline for their

weighing instruments and, as a result, has reduced maintenance and

calibration service costs by 27%.

Process breakthroughTheraSkin is a Brazilian company with more than 13 years of experience in the development and production of skin treat-ment products. Based on scientific prin-ciples, GWP (a method for risk manage-ment and quality control introduced by METTLER TOLEDO), mapped the applica-tion of 78 balances used by TheraSkin in its laboratory in order to analyze specific instrument risks and their subsequent cor-relation with process tolerances.

For Marcos Vinicius, TheraSkin’s instru-mentation technician, the adaption of

GWP meant an overhaul of the entire sys-tem. “There was even a reformulation of internal processes, including a change of habits and culture. Now, all balances have tags that inform the operator of the mini-mum weight that each one supports. This has certainly resulted in a processes break-through for our company," he explains. Weighing without riskThe GWP methodology analyzed the five stages in the life cycle of a balance, start-ing with a full evaluation of weighing process characteristics. This included po-tential risks of inaccurate measurements

and process impacts in regard to relevant regulations or standards. From this sur-vey, the guideline further provided spe-cific and individual recommendations for each balance including information about minimum weight, required tests for routine checks, regularity of checks and calibra-tions, standard weights required and the control limit for each test. The entire pro-cess, from data collection, analysis, pre-sentation, implementation and training, lasted three months.

"All balances were submitted to consistency analysis with the procedures we perform

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5METTLER TOLEDO Cosmetics, Fragrances, Food Ingredients 3

here. The study highlighted that the bal-ances we maintain and calibrate every three months could have this period ex-tended to every six months and, similarly, those maintained and calibrated every six months extended to once a year,” says Pasquale Loiacono, TheraSkin’s engineer-ing and maintenance manager.

"GWP identified that our calibration pe-riod and the frequency of periodic verifi-cation tests were overestimated. The tool was applied to TheraSkin’s installed base resulting in time and labor reductions as the total plant’s balance checking time

was greatly reduced, increasing equipment production time,” explains Daniela Soares, METTLER TOLEDO’s Laboratory Division product manager.

Pasquale Loiacono said that the metho-dology created by METTLER TOLEDO pro-vided the missing reference to justify the weighing equipment used by TheraSkin. "There weren’t specific technical standards directing us towards the regularity of checks and calibrations, except for the balance manufacturer’s recommendations. GWP helped us to determine the range of tests, weights required for each balance, correct tests and tolerances for regular checks and systematic tests that minimize risks and reduce costs. We have adopted all of these GWP recommendations as our standard operating procedures,” said the engineer, adding that, "the method was so effective for laboratory processes that, in June, AN-VISA (National Agency for Sanitary Vigi-lance) carried out their inspection in oc-currence with Good Practice of Production. They were not able to identify any instances of non-compliance regarding weighing processes.”

www.mt.com/GWP

About TheraskinTheraSkin is a 100% Brazilian company developing and producing skin care prod-ucts. The company has been operating for more than 13 years offering products for

Know-how

Weighing Without Risks with GWP®

Good Weighing Practice™ (GWP®) is an easy to follow guideline for handling weighing instruments in a quality man-agement system. With GWP® you can minimize measurement errors and achieve easy compli-ance with little effort.

Uncertainty is all around us and can play a critical role, espe-cially in weighing. Keeping your instruments under control is a challenge and can be costly if handled incorrectly. GWP® minimizes weighing risks and assures the consistent high qual-ity of your weighing processes.

Watch the video demonstration on how measurement errors can be minimized with GWP® guide-lines:

www.mt.com/gwpthe treatment and prevention of acne, me-lasma, dermatitis, hives, rashes, psoriasis, miliaria, mycosis, onychomycosis and der-matophytoses in addition to other products, including creams for moisturizing and anti-aging, with a portfolio of 36 products. Today, their laboratory is ranked the second most prescribed laboratory in this market and 8th position in terms of revenue in this market segment.

The GWP® Verification summary report fea-tures a gap analysis between the required accuracy and the actual weighing perfor-mance.

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S80 Ion/pH Meter with electrode arm

METTLER TOLEDO Cosmetics, Fragrances, Food Ingredients 3

In the Food and Beverage division of a leading global flavor and fra-

grances manufacturer, they need to measure salt and sodium content

of their food products. They sought to upgrade laboratory equipment

and wanted to take advantage of the latest technological advances to

provide consistency in their production batches and uniformity in its

processes.

S80K Dual-Channel Meter PromisesPrecise Salt and Sodium Measurements

Evaluating salt and sodium contentBefore contacting METTLER TOLEDO, the manufacturer was using a series of older electrochemistry meters to test their food products for salt and sodium levels separately.

With thousands of employees around the globe, the customer was looking for a practical and precise electrochemistry meter system that could help to standard-ize their laboratory practices.

Cutting edge technology standardizes processMETTLER TOLEDO recommended a SevenMulti™ S80K Dual-Channel (pH/ion) Meter with chloride and sodium ion-selective electrodes to allow the manu-facturer to perform concurrent measure-ments of both elements in a single sample or individual analysis of each ion.

By working with METTLER TOLEDO, the manufacturer collaborated with an in-ternational company that provides a de-pendable, globally available product and a vast support network of accessible and knowledgeable scientific experts.

This leading flavor and fragrance manu-facturer is now using the meter system to test liquid ingredient mixes for a variety of products, such as soups, beverages and ingredient concentrates.

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In order to ensure they meet customer specifications, the company wanted to be able to perform both salt and sodium content tests using a single electrochem-istry meter and combination ion-selective electrodes. Ion-selective electrodes are used for determining the concentrations of various ions in solutions where the sol-vent is water. Periodically, the company also wanted to evaluate the pH (measure of the acidity) level of their samples.

S80 Display Detail

7METTLER TOLEDO Cosmetics, Fragrances, Food Ingredients 3

Tips & Tricks

Finding the right pH electrode

The measurement of pH value is a vital task in the cosmetic, flavors and food ingredients industries. It can be a challenge to find the appropriate pH electrode for the specific needs in the different applications.

METTLER TOLEDO's web based pH Electrode Selector Guide assists in selecting the most appropriate electrode for every application.

Just a click on the desired segment, e.g. ‘Cosmetic or viscous samples’, provides an instant overview of the electrodes that cover the majority of these applications.

Find the right electrode quickly and easily; including technical data sheets and cable guide.

www.electrodes.net

Along with its ease-of-use, the company was impressed with the accuracy, effi-ciency and reproducibility of a METTLER TOLEDO meter system. By using a METTLER TOLEDO system, they are able to increase sample throughput by analyz-ing samples at a faster rate while provid-ing a quicker response time for making necessary product adjustments.

With customers including major interna-tional manufacturers of the world’s best-known brands, the manufacturer knows its flavors and fragrances must be able to compete in a highly competitive industry. By utilizing METTER TOLEDO’s accurate, fast and indispensable meter system, they will prove to be a fierce competitor in the global marketplace.

www.mt.com/phmeters www.mt.com/phlab_sensors

Each buffer solution from METTLER TOLEDO comes with a quality inspection certificate which guarantees the stated values and traceability.

8 METTLER TOLEDO Cosmetics, Fragrances, Food Ingredients 3

Prod

uct N

ews

Rainin’s new Pipet-Lite XLS family of

pipettes brings precision, ergonom-

ics and asset management to an

entirely new level. With their sure-fit

handles, lighter springs and Rainin’s

patented Magnetic Assist technol-

ogy, these high-performance instru-

ments are designed for ultimate

comfort and ergonomic safety. They

are also the world’s first pipette

family to be equipped with RFID tags

for advanced calibration and asset

management.

Pipet-Lite™ XLSThe Best in Class Just Got Better

Single-Channel Multichannel8 and 12 channel

Adjustable Spacer6 and 8 channel

Channel consistencyRainin’s patented LTS LiteTouch System assures a leak free seal on every channel, and with minimal force. With no nozzle o-rings, Pipet-Lite XLS’s design eliminates a weak point and potential source of contamination.

Ultimate flexibilityWith our Adjustable Spacer model, all it takes is a simple twist of a dial to quickly move samples between different plate and tube formats.

www.mt.com/PipetLiteXLS

Secure volumes The improved snag-proof design allows rapid volume adjustment even when wearing gloves, preventing acci-dental volume drift and delivering con-sistent results.

9METTLER TOLEDO Cosmetics, Fragrances, Food Ingredients 3

Determining the moisture content

of powdered flavors and aromas

is essential in order to maintain

consistent quality between batch-

es and avoid agglomeration.

New One Click™ Loss on Drying,

powered by LabX, simplifies and

speeds-up the standard reference

method using the drying oven.

Full user guidance, automatic da-

ta handling and intelligent sample

handling make the process fast,

easy and secure.

One Click™ Loss on DryingPowered by LabX

www.mt.com/1-click-weighing

One Click™ StartEnter number of samples, print labels.

Label crucibles and precondition in oven.

WeighScan crucible ID and weigh empty.

Enter sample ID, use SmartTrac to weigh samples.

DryDry samples in oven then cool in desiccator.

Resume task on balance and weigh dried samples.

Result Calculation % moisture calculated automatically.

Green screen indicates result is within specified tolerances.

DocumentationEverything is automati-cally recorded.

Customized reports can be printed at any time.

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One Click™ Loss on Drying – Powered by LabXSimply start the method with the One Click™ shortcut on the balance touchscreen. LabX guides the user step-by-step through the SOP on the balance and handles all data automatically. Intelligent task manage-ment makes it easy to run several series simultaneously. The complete solution can be tailored to match individual requirements.

10 METTLER TOLEDO Cosmetics, Fragrances, Food Ingredients 3

Onl

ine

Tool

s Benefit From our Knowledgefor Your Best Practices

Good Titration Practice™

Risk Checker Diverse factors can affect titration results. Spend 5 minutes answering 8 questions, determine titration result reliabilty and save money.

MovieSee how One Click Titration™ simplifies daily lab work with its uniform and intuitive user concept.

BrochureOrder a copy of the 'Good Titration Practice™' brochure. The brochure focuses on how to perform optimum titration analysis.

www.mt.com/gtp

Good Weighing Practice™

Discover the 5 major steps to Good Titration Practice™. Comprehensive support and guidelines ensure the financial and time investment are always allocated appropriately whilst minimizing risks.

Risk Checker Find out if your results are continuously accurate. Simply answer 8 questions and the GWP® Risk Check provides expert advice on how to optimize system quality.

MovieWatch our educational videos and learn more about how GWP® ensures weighing result accuracy.

WebinarsAttend our ‘Measurement Uncertainty & Minimum Weight’ and ‘Routine Testing of Weighing System’ interactive GWP webinars to learn more on how to establish and maintain a sustainable quality of weighing processes.

www.mt.com/gwp

GWP® (Good Weighing Practice™) is a risk-based approach that clearly interprets the regulations of each industry and puts them into straightforward weighing practice. This results in a simple program that assures compliance at any time.

Discover 4 selected best practices from METTLER TOLEDO in mt.com. Profit from the

useful, valuable yet free know-how from our experts to support your daily laboratory

procedures and minimize risk of measurement.

11METTLER TOLEDO Cosmetics, Fragrances, Food Ingredients 3

Good Crystallization Practice

Improving Laboratory CrystallizationFocusing on laboratory case studies, key stages in the process development lifecycle where the implementation of FBRM® and PVM® has been successful are demonstrated.

Improving Production CrystallizationDiscover five high impact ways to achieve ideal crystallization production conditions. Special attention is paid to the most common ‘problem areas’ encountered in production crystalli-zations where the implementation of FBRM® has proven useful.

Crystallization WebinarsA variety of on demand crystallization webinars are available on our internet portal. The webinars support you in development, optimization and scale-up of your crystallization processes.

www.mt.com/crystallization

Good Moisture Practice

Scale-up of crystallization is notoriously complicated and companies are under pressure to develop scalable crystalliza-tion processes faster, at lower costs and with higher quality. Companies must control crystallization conditions in order to improve cycle times and optimize product quality.

Method DatabaseExplore the database that contains more than 100 methods referenced to an official moisture determination method (e.g. drying oven).

Industry SolutionsExperience complete solutions for moisture analysis in a variety of industries, including sugar, plastics and wastewater.

Moisture Measuring PrinciplesLearn more about the method of drying (heating the sample using thermal radiation) and the principle of the switch-off criterion.

www.moisture-guide.com

Moisture determinations need to be carried out reliably and quickly so that any interventions in the production process can be made promptly in order to avoid interruptions. Our website offers a valuable Moisture Guide method database and industry solutions which can help to save precious development time.

www.mt.comFor more information

Mettler-Toledo AGLaboratory DivisionIm LangacherCH-8606 Greifensee, Switzerland

Spotlight on Promotion

Correct and accurate pH measurements depend upon reliable instruments and electrodes. Right equipment selection, handling and maintenance are crucial for obtaining optimum results and prolonging instrument lifetime.

The "pH Theory Guide" from METTLER TOLEDO provides:– Clear and useful descriptions of how pH is measured in the laboratory environment.– Comprehensive pH applications.– Abundant tips and hints to assist daily tasks.– Practical electrode selection guidelines, storage

and maintenance tips.– Trouble shooting.

Simply sign up to receive a free hard copy of our pH Guide

www.mt.com/lab-pHGuide

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A Guide to pH Measurement –

the theory and practice of laboratory pH applications

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Comprehensive Guidefor Optimum pH Measurement