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Muhammad Ashfaq
Pakistan barcode project:progress and applications
2010: Barcoding economically important insect species from Pakistan
Biodiversity Institute of Ontatio National Institute for Biotechnology and Genetic Engineering (NIBGE)
Funded by: HEC
Project execution
Manual catching Pipettes and sieves
Arial nets Light traps Shaking onto sheets
Activities and Progress
Collecting animal parasites
Specimen preparation and databasing
SpecimensAnimals: 34103Plants: 1289
BarcodesAnimals: 28605Plants: 988
IDRC (62%)
18 months
Pakistan-only BINs/ species
II. Plants: 363
Number of BINs/ species
II. Genera: 698
Taxonomic identifications
III. Species: 764
Barcode Applications
Reference libraries
Helicoverpa armigera
Helicoverpa zea (GU087831)
Heliothis virescens (GU336572)
Masalia sp.
Heliothis sp.
Heliocheilus confertissima
Spodoptera littoralis
Spodoptera litura
Spodoptera cilium
Spodoptera exigua
Mamestra sp.
Mythimna sp.
Leucania venalba
Mythimna convecta
Agrotis segetum
Agrotis ipsilon
Chrysodeixis acuta
Thysanoplusia orichalcea
Chrysodeixis eriosoma
Ctenoplusia albostriata
Trichoplusia ni
Hypocala deflorata
Pandesma robusta
Dysgonia torrida
Achaea janata
Earias insulana
Earias vittella
Pectinophora gossypiella
53
99
90
90
29
30
89
92
72
41
91
2
21
21
41
18
19
98
33
73
24
23
5
86
49
Heliothinae
Amphipyrinae
Hadeninae
Noctuinae
Plusiinae
Catocalinae
Chloephorinae
Gelechiidae
Noctuid species in/around cotton fields of Punjab
Previously only S. litura was reported from cotton in Punjab
Genetic diversity:
mango mealybug, Drosicha
mangiferae
Bt- 1 (36) [AAM1247] Asia II 1
Bt- 2 (1) [ AAM1246]
Bt- 3 (2) [ACB7718]
Bt- 4 (5) [ AAM1248] Asia II 7
Bt- 5 (2)
Bt- 6 (8) [AAM1243]
Bt- 7 (11) [AAM1245] Asia II 5
Bt- 8 (15) [AAM1244] Asia 1
Bt- 9 (2)
Bt- 10 (2) [AAA4495]
Bt- 11 (13) [AAT8875] MEAM1
Bt- 12 (19)
99
99
99
99
99
97
99
99
59
99
84
63 99
52 58
98
29
40
99
91
0.02
Bemisia tabaci, a complex of cryptic species
E
W
N
S
(A) Asia II 1
(B) MEAM 1
(C) Asia 1
Bemisia tabaci, distribution analysis
Dengue epidemic in Punjab
Culex fuscocephala (5; 0.6) Culex perexiguus (1; -) Culex bitaeniorhynchus (4; 0.15) Culex mimeticus (1; -)
Lutzia fuscana (2; 0.0) Culex tritaeniorhynchus (84; 2.01) Culex quinquefasciatus (1025; 1.05)
Culex theileri (3; 0.0) Armigeres subalbatus (45; 0.31)
Culicidae (5; 0.6) Aedes MA03 (3; 0.9)
Aedes MA02 (9; 1.0) Aedes MA04 (45; 1.4)
Aedes aegypti (48; 0.5) Aedes albopictus (175; 1.3)
Aedes MA01 (6; 0.9) Aedes w-albus (10; 0.9) Ochlerotatus pseudotaeniatus (5; 2.0)
Ochlerotatus sp. (2; 0.0) Anopheles subpictus (39; 1.4)
Anopheles peditaeniatus (8; 0.8) Anopheles pulcherimus (8; 0.3)
Anopheles splendidus (1; -) Anopheles stephensi (29; 0.4)
Anopheles sp. nr. dravidicus (1; -) Anopheles annularis (3; 2.4)
Anopheles culicifacies (5; 0.6) Anopheles culicifacies A (1; -)
Mansonia bonneae (1; -) Chironomus kiiensis
100 49
31
13
10
31
13
96
65
62
73
55
41
28
34
31
91
24
21
22
7
8
14
6
35
2
0
0.02
Cule
x Ae
des
Anop
hele
s
Mosquito species in Punjab
Genus: Anopheles Genus: Culex
Genus: Aedes Aedes aegypti Aedes albopictus
Genus-wise distribution of mosquitoes in Punjab
Outreach
Barcoding project at NIBGE
Barcoding team members
Thank you for your attention