MONITORING LARVAL/JUVENILE LAMPREY THROUGH … Info Excha… · •Type 1 habitat preferred for habitat survey •Survey Type 2 only if Type 2 habitat is x10 more abundant than Type

MONITORING LARVAL/JUVENILE LAMPREY THROUGH ELECTROFISHING

SURVEYS – ARE YOU ‘SHOCKED’ TO HEAR THE VARIOUS METHODS/

PROTOCOLS AVAILABLE?

Dave’y Lumley

Lamprey Biologist I

Yakama Nation Fisheries Resource

Management Pacific Lamprey Project

December 12, 2019

Yakama Nation

Project Goal

To restore natural production to a level that will

provide robust species abundance, significant

ecological contributions, & meaningful harvest

throughout the Yakama Nations Ceded Lands & in

the Usual & Accustomed areas.

THE LAMPREY TEAM

Tyler Beals

Ralph LampmanShekinah SaluskinDave’y Lumley

Mildred JonesSean Goudy

PACIFIC LAMPREY PROJECT

• Currently

• Adult translocation, tagging,

overwinter holding and monitoring

• Artificial Propagation, larval rearing

and monitoring at Prosser Hatchery

• Larval habitat surveys

• Educational outreach

MAIN FOCUS LARVAL HABITAT SURVEYS

• Todays Talk

• Overview of YN larval habitat sampling protocols, preparation and data

collection

• Summary of questionnaire responses from different agencies from around

the world

• Objective

• Help to show the diverse sampling protocols used for larval lamprey

WHY FOCUSING ON LARVAE IS USEFUL?

• Species from around the world have different adult life ectotypes but share

similar larval characteristics

• Adults and juveniles are hard to monitor because they are not present year

round (vs larvae present year round)

• Short and long term population demographic availability

• 3-9 generations of lampreys can be found within one site

E-FISHING QUESTIONNAIRE

Name Agency Location

Lamprey Team YN FRMP PLP Washington, USA

Aaron Jackson CTUIR OR/WA, USA

Doug Nemeth / John

Erhardt USFWS/NPT Idaho, USA

Joe Skalicky USFWS CRFWCO OR/WA, USA

Jeff Dreier

WA State Dept of

Transportation Washington, USA

Eli Felts IDFG Idaho, USA

Ben Clemens ODFW Oregon, USA

Jenni Schmitt South Slough NERR Oregon, USA

Fraser Neave Fisheries and Oceans Canada

Great Lakes Region,

Canada

Robert Frank

USFWS (Marquette Biological

Station)

Great Lakes Region,

USA

Nick Johnson

USGS Great Lakes Science

Center

Great Lakes Region,

USA

Pedro Almeida et al. MARE University of Evora Portugal

Kimmo Aronsuu

FELY Centre for North

Ostrobothnia Finland

Hiroaki Arakawa Ishikawa Prefecture University Japan (Ishikawa)

Cindy Baker NIWA New Zealand

• 16 question questionnaire about sampling

protocols sent out to different Agencies

from around the world

• 16 responses

• United states (10)

• Canada (1)

• Portugal (1)

• Finland (1)

• Japan (1)

• New Zealand (1)

• Russia (1)

Most entities list

relative abundance

(80%) & distribution

(60%) as a key goal

for the surveys

REACH SIZE

• The Reach size depends on what project

is currently being worked on and the

gear included

• Examples:

• Deep water shocking

• Surveying Type 1 and Type 2 transects

• WSDOT culvert/bridge size

• Results in a wide size range of habitat

being sampled

• 50-2000 m

~50 m

34%

~100 m

13%400-800 m

13%

1,000-2,000 m

13%

Undefined

20%

Variable

7%

REACH SIZE/SCALE

E-FISHING TIME AND PACE

<10 mins

6%

10-20 mins

27%

21-30 mins

0%

31-40 mins

27%

41-60 mins

0%

61-70 mins

7%

Variable

20%

Undefined

13%

SAMPLING TIME (MIN)~30 sec/m2

7%

~60 sec/m2

20%

30-90 sec/m2

13%

~90 sec/m2

13%

~120 sec/m2

7%

(~5143

sec/m2)

7%

Undefined

33%

E-FISHER SURVEY SPEED (SEC/M²)• Sampling time

varies from 6-

70 mins

• Pace

varies from

30-5143

secs/m²

Total electrofishing time Time spent electrofishing

1 m²

E-FISHING TIME AND PACE

<10 mins

6%

10-20 mins

27%

21-30 mins

0%

31-40 mins

27%

41-60 mins

0%

61-70 mins

7%

Variable

20%

Undefined

13%

SAMPLING TIME (MIN)~30 sec/m2

7%

~60 sec/m2

20%

30-90 sec/m2

13%

~90 sec/m2

13%

~120 sec/m2

7%

(~5143

sec/m2)

7%

Undefined

33%

E-FISHER SURVEY SPEED (SEC/M²)

• 53% spend

10-40 mins for

their total

sampling time

• 43% have a

pace between

60-90 sec/m²Finland uses a sifting

method

HABITAT SURVEY TIME

<1 hr

20%

0.5-2 hrs

27%

2-3 hrs

20%

3-4 hrs

6%

>4 hrs

7%

Variable

13%

Undefined

7%

TOTAL SURVEY TIME

1

67%

2

13%

Multiple

13%

N/A

7%

SURVEY PASSES

HABITAT SURVEY TIME

<1 hr

20%

0.5-2 hrs

27%

2-3 hrs

20%

3-4 hrs

6%

>4 hrs

7%

Variable

13%

Undefined

7%

TOTAL SURVEY TIME

1

67%

2

13%

Multiple

13%

N/A

7%

SURVEY PASSES

• Graph from electrofishing study

by Warm Springs Tribe

• Caputred 75-95% of the lamprey

from 1st pass (45 seconds of e-

fishing).

• With 4 (180 seconds) and 8 (360

seconds) times more shocking,

you can certainly narrow this

down to 90-100%, but is that extra

time really worth that level of

refinement you get? 95

75

100

90

SHOCKER TYPE AND VOLTAGE

• Majority of agencies are using the EST

ABP-2 (60%)

• Other models listed include:

• Smith-root LR-24


• Hans Grassel EL 62 Generator (DC)

• NIWA Kainge EFM300

Primarily 125

47%

100-200

13%

200-300

27%

300-600

13%

SHOCKER VOLTAGE

SHOCKER TYPE AND VOLTAGE

• Majority of agencies are using the ABP-

2 (60%)

• Other models listed include:



• ETS ABP-2

• Hans Grassel EL 62 Generator (DC)

• NIWA Kainge EFM300

Primarily 125

47%

100-200

13%

200-300

27%

300-600

13%

SHOCKER VOLTAGE

EXTRAPOLATION

Yes

72%

No

21%

N/A

7%

MISSED LAMPREY COUNTED

Yes

27%

No

73%

VISIBILITY MEASURED

BIOLOGICAL DATA

• Varies by project

• Majority measure all

• Some by species

• Some by total number captured

• Some none at all

None

6%10

7%

50-100

13%

All

67%

Variable

7%

TOTAL LAMPREY MEASURED

WHERE WE SURVEY

• We survey the subbasins within the

Yakama Nation Ceded Lands

• Yakima

• Wenatchee

• Entiat

• Methow

• Klickitat

• White Salmon

• Survey time

• July to October

GOALS

• Presence/Absence/Distribution

• Long term monitoring of status

• especially in relation to translocation

• Relative abundance

• # & mass density, CPUE, 50 m reach # & biomass

• Biological data

• Size classes and species composition

YAKAMA NATION LARVAL HABITAT SURVEY PROTOCOLS

Step 1: locating the Type 1 Habitat

• Google Earth - Finding suitable habitat

• Aerial imagery documents change over time

• Finding stable Type 1

• Help to find access

• Public land

• Private land

1994 Yakima River

2006 Yakima River

2019 Yakima River

Main ChannelChannel Edge

AlcoveSide Channel

Large Wood Boulders

Large Wood

Restoration Projects

Artificial Structures

(Diversion Screens)

Habitat Forming Features

Satus Creek

Satus Creek

Light brown fine sediment, slow moving water, inside meanders or widening channels

STEP 2: SUPPLIES

• Shocker backpack, arm nets, cables

• Charged batteries for shocker (2 batteries - main and

spare)

• Spare fuses

• 5 gallon buckets or mesh laundry baskets

• Plug in and portable aerator with stones

• 2 long handled dip nets with fine mesh

• Small tub or bucket for knocking out fish

• aquarium dip nets

• Small and large photoarium

• Fine weight scale with extra batteries

• MS-222 diluted

• Fish ID photos

• Thermometer

• Water Quality Reader

• Yard stick

• Camera

• GPS

• Rite in the Rain Datasheet with pencils

• Genetic sheets with small sharp scissors

• Site maps

• Flagging tape

• Small transect tape

• Sharpie

• Heavy rubber gloves

• Machete

• Allen wrench (for set screw tightening if needed)

• Flat screw driver

• Waders and boots

ABP – 2 Backpack ElectrofisherETS Electrofishing, Madison, WI

Slow Pulse (“Tickle” Charge)• Rate = 3.0 Hz (pulse per second)• Duty Cycle = 25%• Encourage lamprey to escape their

burrows.

Rate (Hz) – Bursts per second

Duty Cycle – The ratio of “on” time to period,expressed as a %


Slow Pulse (“Tickle” Charge)• Rate = 3.0 Hz (pulse per second)• Duty Cycle = 25%• Encourage lamprey to escape their

burrows.

Fast Pulse (“Stun” Charge)• Rate = 30.0 Hz (pulse per second)• Duty Cycle = 25%• Stun exposed/swimming lampreys


Duty Cycle – The ratio of “on” time to period, expressed as a %


Slow Rate (“Tickle” Charge)• Rate = 3.0 Hz (pulse per second)• Duty Cycle = 25%• Encourage lamprey to escape their

burrows.

Fast Rate (“Stun” Charge)• Rate = 30.0 Hz (pulse per second)• Duty Cycle = 25%• Stun exposed/swimming lampreys

Voltage and Burst Rate• 3:1 Burst Rate• Voltage = 125• Increase voltage up to 200 if temp

<10 C


Duty Cycle –The ratio of “on” time to period, expressed as a %



Duty Cycle The ratio of “on” time to period, expressed as a %

Slow Rate (“Tickle” Charge)• Rate = 3.0 Hz (pulse per second)• Duty Cycle = 25%• Encourage lamprey to escape their

burrows.

Fast Rate (“Stun” Charge)• Rate = 30.0 Hz (pulse per second)• Duty Cycle = 25%• Stun exposed/swimming lampreys

Voltage and Burst Rate• 3:1 Burst Rate• Voltage = 125 – 200 V• Increase voltage with drop in

temperature (<10 C).

Voltage / AMP Monitoring• PKC option = Amperage• PKV option = Voltage• Battery option = battery level (V)


STEP 3: AT SITE

• Fill out site data

• Choose ‘best’ 50 m reach

• Quantity and quality of Type 1 habitat

• Estimate total Type 1 and Type 2 habitat

(in 1 m² increments)

• If total area not visible, extrapolate

using best professional judgement

Type 1

Type 2

Type 3

Type 1: PREFERRED.

Consists primarily of

a mixture of fine

sediment and

organic

matter/detritus

Type 2: ACCEPTABLE.

Mixture of fine &

coarse substrates or

shifting sand

Type 3: NOT

ACCEPTABLE. All

coarse substrate. No

fine substrate.

• Type 1 habitat preferred for habitat survey

• Survey Type 2 only if Type 2 habitat is x10 more abundant

than Type 1

• Based on our field data that indicates larval density on

average are x10 more in Type 1 vs Type 2

• Sea Lamprey research did a study and found similar results

• If YOY present, fill out column, if not, ignore YOY column

Type 1 YOYType 2

VISIBILITY

• % visibility is based on

what % of the volume of

water can you see within

where you are shocking

• Can be impaired by:

• Aquatic vegetation

• Turbid water

• Sun glare

• Large organic matter

• Wind

• Fast moving water

• precipitation

• Water Quality

• Conductivity (µS)

• TDS (ppm)

• pH

• Voltage and Amperage

• AMP readings at 125 V

• Voltage required to reach 1 AMP

• Started this year to increase

baseline data to better

understand the relationship of

conductivity & AMP & capture

efficiency

• Genetic Summary

BIOLOGICAL DATA

• Count and sort by species and age to

determine lamprey measure ratio

• Total Weight of each group

• Measure 50 total lamprey

• Length

• Weight on representative three

• PA, WB, unknown

• Species

• YOY if <35 mm

• Unknown if <50 mm

• Pacific

• WB (A/B tail morphology)

• Photos

• Genetics

• Comments

QUESTIONS?

Dave’y Lumley

[email protected]

(509)480-8596

mailto:[email protected]

Documents

MONITORING LARVAL/JUVENILE LAMPREY THROUGH … Info Excha… · •Type 1 habitat preferred for habitat survey •Survey Type 2 only if Type 2 habitat is x10 more abundant than Type