176 A b s t r a c t s

C-9.1 #235

MONITORING INDIVIDUAL TECHNOLOGIST TRAY READING FOR MONTHLY QUALITY CONTROL. FA~ar Coooer, Dod Stewart, Ochsner HLA Laboratory, New Orleans, LA.

To standardize and monitor the scoring of cytotoxic reactions in HLA typing trays among technologists and address some ASHI and CLIA requirements for internal QC of technologists a computer analysis has been developed.

The program compares all technologist's scoring of each well. If the score lbr an individual well reaches consensus (51% agree), the numeric score assigned by each tech is compared to the consensus score. The score is recorded as concordance (a hit), miss high (over-scoring), or miss low (under- scoring). The analysis could compare either a single tray read by all technologists or a complete test in which the tray and cell were prepared, plated and run on an individual basis.

The analysis is very helpful in detecting patterns of over-scoring or under-scoring. Using this analysis, we are able to monitor and assess the technologists ability to record reactions consistently, thereby eliminating possible problems with inconsistent typing tray and crossmatch reading.

The program is now run in a dbase environment but is being converted to the C programming language. Then it can be run on any IBM compatible machine. It will use the ASCII file formats created by the Robbins Autoscope support program: SCOPESCR. In addition, an ASCII file consisting of a trayid, tech id and scores in a predefined format can also be used (when the Robbins' Autoscope program is not available).

MISS MISS TECH TRAYID VIA SCORES HIGH LOW HIT

1 MD-3 97%8181118811668888886 ...... 888111111811121 10 0 61 2 MD-3 99%8181118811118888861 ...... 881111111611111 3 0 68 3 MD-3 99%8181118611118888181 ...... 881111111111111 0 0 71 4 MD-3 99%8181118611118888121 ...... 881111111411111 0 1 70 5 MD-3 99%8181118611118888181 ...... 881111111211111 0 0 71 6 MD-3 99%8181118611118828181 ...... 481111111111111 0 2 69

col~ensus 8181118611118888181 ...... 881111111x11111