Nanostructured Surfaces:

Development of a New MS-Platform to Capture,

Process and Analyse (CPA-MALDI)

Urine-Derived Biomolecular Compounds by

Monica Epifanio (monica.epifanio@dcu.ie)

Marie Curie fellow at Tethis S.p.A (Milan, Italy) and Dublin City University (Dublin, Ireland)

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SHOTGUN PRESENTATION

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To develop a new diagnostic technology based on:

Smart Nano-structured surfaces;

Suitable for MALDI -MS;

Micro-fluidics.

Aim of my work

Key challenges in proteomics-based clinical diagnostics

Continuous need and wish for higher sensitivity

Ability to analyse complex samples

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Our solution… Nanoparticle Beam Deposition system

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UNWASHED WASHED

UNWASHED WASHED ID=2 mm

6 microliters

Sample concentration and capturing

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NS-COATED PLATE

MSI Mass Spectrometry Imaging

VI. Data processing

III. MALDI analysis

V. Image reconstruction

II. Transfer on ITO+TiO2 slide and matrix deposition I. Sacrifice and organ dissection

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http://www.tethis-lab.com/

PEPTIDES PROTEINS

Trypsin digestion

CPA-MALDI

2D

Capturing-Processing-Analysing

1D

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Acknowledgment Dr. Emanuele Barborini Dr. Marc Bauman Dr. Alexander Yavorskyy Mr. Simone Vinati

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Mr. Giacomo Bertolini

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