Molecular response of Deinococcus radiodurans to simulated microgravity explored by

proteometabolomic approach

Emanuel Ott, Felix M. Fuchs, Ralf Moeller, Ruth Hemmersbach, Yuko Kawaguchi,

Akihiko Yamagishi, Wolfram Weckwerth and Tetyana Milojevic

*Corresponding author

tetyana.milojevic@univie.ac.at

Supplementary Information:

Supplementary Figure 1: Heatmap of normalized LFQ intensities from significantly different

expressed proteins identified in every replicate.

Supplementary Figure 2: Results of protein and peptide quantification photometric assays for

sample normalization.

Supplementary Table 1: Raw protein LFQ intensities, corresponding statistical analysis,

number of identified peptides and calculated Maxquant score.

Supplementary Table 2: Targeted metabolomics approach with normalized peak areas and

corresponding statistical analysis.

ctrl1

ctrl2

ctrl3

ctrl4

ctrl5 ug

1

ug2

ug3

ug4

ug5

Q9RSL0Q9RRA3Q9RS87Q9RV30Q9RR73Q9RZ77Q9RYY9Q9RUD0Q9RT26Q9RSQ6Q9RRT6Q9RR70Q9RSA6Q9RT62Q9RVJ0Q9RY49Q9RUT7Q9RTQ7Q9RWH6Q9RWC1Q9RUL7Q9RX33Q9RVJ3Q9RT72Q9RUY2Q9RXV6Q9RXK6Q9RSC5Q9RTT6Q9RVG1Q9RUB0Q9RRV8Q9RV18Q9RWB0Q9RU57Q9RUW4Q9RT42Q9RWN5Q9RTQ6Q9RT60Q9RZ07Q9RV70Q9RTA9Q9RRM6Q9RZT2Q9RTQ0Q9RXD5Q9RXT4Q9RUZ9Q9RTM4Q9RZL4Q9RYN6Q9RZD1Q9RRX5Q9RUG2Q9RRK0Q9RSV3Q9RV84Q9RXY9Q9RW95Q9RVP1Q9RR87Q9RZE6Q9RZ24Q9RRE5Q9RT44Q9RZE9Q9RWP9Q9RXL1Q9RYH8Q9RS86Q9RVU2Q9RRR7Q9RRW1Q9RSQ5Q9RSD0Q9RSJ4Q9RWN6Q9RS28Q9RTN5Q9RYS7Q9RX25Q9RRV3Q9RYL8Q9RTY4Q9RXQ3Q9RZT0Q9RX54Q9RVW8Q9RXW4Q9RZI5Q9RZ06Q9RT05Q9RTF5Q9RTK2Q9RWB9Q9RXG1Q9RZ11Q9RTD1Q9RUD9P52027

Q9RW85Q9RX89Q9RUE0Q9RT36Q9RWU2Q9RX52Q9RV56Q9RTZ4Q9RU62Q9RZ99Q9RRS5Q9RXB0Q9RWI2Q9RZS5Q9RWI3Q9RWI0Q9RUC8Q9RZS7

−2 0 2Row Z−Score

Figure S1: Heatmap of normalized (z-scored) LFQ values of significantly different expressed proteins identified in every replicate. The minimum requirement for an identification was one unique peptide.

Figure S2 A) Protein concentration in µg/µL of each replicate (1:5 diluted) against a BSA

standard in different concentrations. B) Peptide concentration in ng/µL of each replicate,

measured against a standard peptide digest solution.