Molecular MotorsArafath Hussain

Stevens Institute of TechnologyDr. Arthur Ritter

Needs and Background

• NIRT Application - Nanoscale Interdisciplinary Research Team

• 15 undergraduate BME students• Nano-scale autonomous device• No academic credit

What is Molecular Motor?

• Chemical energy into Mechanical work - Nanometer scale

• Examples of Motors: - Kinesin - Myosin - Dynein

Objective and Materials

• Develop a platform of Nano devices in an environment

• Hood• Flagella• Petri dish• Plates• Autoclave• Flask

• Incubator• Agar media• Cells

Procedure

• Weigh out the agar

• Autoclave the solution

• Let the flask cool down

• Pour into Petri Dishes

• Spread the algae

• Leave it for couple of days

Results

• Not enough experiments

• More information

• More analysis

• Expected: Get the bacteria with flagella in it.

• "Rotation of the c subunit oligomer in fully functional F1Fo ATP synthase" by Satoshi P. Tsunoda, Robert Aggeler, Masasuke Yoshida, and Roderick A. Capaldi in Proc Natl Acad Sci U S A (2001) volume 98 pages 898–902. Full text at PMC: 14681

• Mitsuya Shirashi, Xuemei Wang, Michael P. Walsh, Gary Kargacin, Kathy Loutzenhiser, Rodger Loutzenhiser. “Myosin heavy chain expression in renal afferent and efferent arterioles: relationship to contractile kinetics and function.” The FASEB Journal. Oct 2003. Fasebj. 16 Oct 2003 < http://www.fasebj.org/cgi/reprint/03-0096fjev1.pdf>

• Isabel Schuchardt, Daniela Abmann,Eckhard Thines,Christian Schuberth, and Gero Steinberg. "Myosin-V, Kinesin-1, and Kinesin-3 Cooperate in Hyphal Growth of the Fungus Ustilago maydis." Mol Biol Cell (2005): 5191–5201.

• Jon Kull, F., et al. "Crystal structure of the kinesin motor domain reveals a structural similarity to myosin." Departments of Biochemistry/Biophysics (Crystal structure of the kinesin motor domain reveals a structural similarity to myosin): 550-555.

• Hirokawa, N. (1998). Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science 279, 519-526

• Vale, R.D. and Milligan, R.A. (2000). The way things move: looking under the hood of molecular motor proteins. Science 288, 88-95.

• Schliwa, M. and Woehlke, G. (2003). Molecular motors. Nature 422, 759-765

• Vale, Ronald D. "The Molecular Motor Toolbox for Intracellular Transport." Department of Cellular and Molecular Pharmacology (2003): 467-480.

References

Acknowledgements

• Dr. Arthur Ritter• Esther• Trupti

• Co: Jae Yoo• Dr. Sat

• Memorial Sloan-Kettering Cancer Center

• Harlem Children Society• And to all of you for listening!