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Molecular Immunology at IGM Who? Where? When? Why and What for?. Gudrun Weiss. 5. floor at IGM at the end of “højhuset”. Where?. When?. January 2009. DTU KU LIFE. Continous Non Stop Process. Molecular Immunology Group. Transformation Process. 1 Associate Professor 1 Post Doc - PowerPoint PPT Presentation
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Place, date, unit, occasion etc.Slide 1
Molecular Immunology at
IGM
Who? Where? When? Why and What for?
Gudrun Weiss
Place, date, unit, occasion etc.Slide 2
DTU KU LIFE
Where?
When?
5. floor at IGM at the end of “højhuset”
January 2009
Place, date, unit, occasion etc.Slide 3
Work Flow
Hanne Frøkiær
Continous Non Stop Process
Transformation Process1 Associate Professor
1 Post Doc
2 PhD Students
2 Lab Technicians
1 Lab Tech Trainee
Molecular Immunology Group
Place, date, unit, occasion etc.Slide 4
Molecular Immunology Group
Stine
Hanne
Louise
Kristina
Who?
Place, date, unit, occasion etc.Slide 5
The Real Engine of the Lab
Anni Shadi Marianne
Place, date, unit, occasion etc.Slide 6
PhD Student Kristina
Galathea Expedition
Marine Bacteria
Collaboration with DTU Aqua
Immune stimulatory effects?
Place, date, unit, occasion etc.Slide 7
Kristina's Winter 2010-11
Place, date, unit, occasion etc.Slide 8
Associate Professor Stine
DNA RNA Protein
miRNA (micro RNA)
Small non coding RNA
-- ug c ug a --------- u
au uua auc cuu guaaaggcgugu uug agg gu c gu c gaccguag u
ua aau uag gaa cauuucugcaca aac a5’
3’
Regulation of Biological Processes
Place, date, unit, occasion etc.Slide 9
PhD Student LouiseUptake of bacteria
in immune cells
Endocytosis
Place, date, unit, occasion etc.Slide 10
Research Topic:Immunological Recognition and Response to Microorganisms
• How are Microorganisms recognized?
• Which Cells interact with Microbes?
• What determines the Response to Microbes?
• How is a Microbe recognized as a Pathogen?
Special Focus: Gastro-Intestinal Tract
Place, date, unit, occasion etc.Slide 11
Why and What for?
Place, date, unit, occasion etc.Slide 12
The Gastrointestinal Tract
• One of the key organs in the body
• Large surface area: 150 - 200 m2
(skin: only 2 m2)
• Surface small intestine:
Size of a Tennis Court
Place, date, unit, occasion etc.Slide 13
The Human Microbiota
Human Gastro-Intestinal Tract
~ 1x1014 bacteria, several thousand different species
• 10 x more bacteria cells than human cells
• 100 x more bacteria genes than human genes
MODE OF DELIVERY
INFANT NUTRITION (Breast or Formula
Feeding)
ANTIBIOTICS GENETIC FACTORS
MICROBIAL EXPOSURE DIETARY FACTORS
COMPOSITION OF THE
MICROBIOTA
MODE OF DELIVERY
INFANT NUTRITION (Breast or Formula
Feeding)
ANTIBIOTICS GENETIC FACTORS
MICROBIAL EXPOSURE DIETARY FACTORS
COMPOSITION OF THE
MICROBIOTA
Place, date, unit, occasion etc.Slide 14
- “Pro Bio” – “For life”
- Gram positive Lactic Acid Bacteria
- Lactobacilli
- Bifidobacteria
- Natural inhabitants of the intestine, “Friendly Bacteria”
- Beneficial Effects: Infectious diarrhea (especially rotavirus)
Inflammatory bowel disease, Lactose intolerance, Allergies
Probiotics
Place, date, unit, occasion etc.Slide 15
Intestinal Tract
Place, date, unit, occasion etc.Slide 16
Dendritic Cells
Excretion of immunstimulatory factors
Immune response in the body
Place, date, unit, occasion etc.Slide 17
+ Growth factor
GM-CSF
Stimulation Assay
8 days: Immature Dendritic Cells
Proteins: Supernatant
• ELISA
Genes: Cells
• Real-Time PCR
• Microarray
Place, date, unit, occasion etc.Slide 18
How do Probiotic Bacteria stimulate the Immunsystem?
Up/Downregulation of Genes in Dendritic cells
Elucidation of Pathways
• Lactobacillus acidophilus
• Bifidobacterium bifidum
Place, date, unit, occasion etc.Slide 19
Lactobacilli
Bifidobacteria
Pro-inflammatory
Anti-inflammatory
Place, date, unit, occasion etc.Slide 20
Inhibition of pro-inflammatory interleukin IL-12- Lactobacillus Acidophilus
- Bifidobacterium Bifidum
Interleukin IL-12
0
0,5
1
1,5
2
2,5
3
3,5
4
4,5
0,1 1 10 20 40 60 80 100
Concentration of B. bifido Z9 (ug/ ml)
IL-1
2 (
ng
/ml)
ELISA
Proteins
Mechanisms?
MICROARRAY
Place, date, unit, occasion etc.Slide 21
L. acidophilus NCFM
Response to Virus
Microarray AnalysisScreening: >34.000 Genes
Place, date, unit, occasion etc.Slide 22
Activation of the Viral Immune System
Expression of the key regulator: Interferon β (IFN- β)
IFN-
0
1000
2000
3000Control
L. acidophilus NCFM
L. acidophilus X37
E. coli nissle 1917
B. bifidum Z9*Poly I:C
*
*
pg
/mL
Protection against influenza?!?
Place, date, unit, occasion etc.Slide 23
Lactobacillus acidophilus Virus + Pathogenic bacteria
JNKpathway
Mechanisms – Use of Knock Out Mice
?dsRNA ssRNA DNA
Place, date, unit, occasion etc.Slide 24
My Winter 2010-11
Place, date, unit, occasion etc.Slide 25
General Atmosphere
in the Lab?
Place, date, unit, occasion etc.Slide 26
Paper rejected
Place, date, unit, occasion etc.Slide 27
Paper accepted
Place, date, unit, occasion etc.Slide 28
Have a nice Weekend!!!
Any Questions???