Molecular Imaging System

General Layout

600 – 900 nm light is used to illuminate fluorescent substances injected into the subject on the image plane.

Optigrid Functionality• Motors provide 120o phase shifts and focus

– Grid moves on the x-axis 11.11 um to complete a phase change

– Improves Clarity– Grid moves 2 mm on the z-axis to focus image– 30 line pair/mm on a 10x10 mm square (line pair

approx .001 in wide)– Lines spatially modulate the illumination

A portion of the OptiGrid at 200xy

xz

Image Sharpening

Provided by Qioptiq

3D Rendering

Provided by Qioptiq

Subsystem BreakdownImaging Unit

Mechanical

Lens Mount Grid Mount

Software

Motor Control

Image Capture

Image Rendering

Optical

Lens System

Medical Testing

Phantom Hand

Optical System Review

Lens Selection Parameters

• Focal Length• Magnification• Box Length = 250 mm• Box Height = 200 mm.• Lens size

Computar C mount

Alternative Lenses:

•Telecentric Lens

•Zoom Lens

•Simple Lens

OSLO Simulation

Simulation by Jeff

Rear Projection

45 degrees

90 degrees

440mm Platen to Lens3mm Lens to Grid

Degrees of Motion

Motion Solution

By Edmund

Construction Aluminum/Stainless Steel

Mounting Surfaces Precision ground

Straight Line Accuracy 2µm/25mm of travel

Position Repeatability ±0.00005"

Configurations X; solid top or thru hole

Drives Metric Micrometer: 0.01mm graduations/0.5mm coarse Fine Screw: no graduations/64 pitch

Load Capacity 3 kg normal (horizontal orientation, force against stage)4.5 kg Ta (vertical orientation, thrust against drive)1.4 kg Tb (vertical orientation, thrust away from spring)

Weight 0.078 kg / axis

NT56-35 by Edmund

Metric Rotary Stages by Edmund

Stock No. #55-028

Coarse Rotation 360°

Total Fine Rotation ±5°

Total Travel/Knob Rotation 70.2 min

Horizontal Load Capacity 3 kg

Weight 90g

250mm

250mm

# Part DescriptionQuantity Vendor

Vendor Part # Price

1 Carestream Box   1 Carestream    

2 OptiGrid   1 QiOptiq    

3 Dovetail Optical Rail 12" Optical Rail 1Edmund Optics NT54-401 99.75

4 OptiGrid Holder   1 QiOptiq 374352-000  

5 Three-Screw Ring Mounts 95mm Adjustable Len Mount 12.5-76mm 1Edmund Optics NT36-605 49.00

6 Small Carrier .5" Dovetail Carrier 2Edmund Optics NT54-403 55.00

7 Metric Rotary Stage 60mm 360 Rotary Stage 2Edmund Optics NT55-028 231.00

8 1/4-20 ScrewsTo screw into rails for mounting grid & lens        

9 1/16" Black Polyurethane 80A Durometer, 6" x 6" 1 McMaster 8716K153 11.74

10 Loctite 404 Quick Set (46551) rubber adhesive, 1/3 oz bottle 1 Ellsworth 46551 17.69

11 Rule printed sticker 12" ruled tape 1 McMaster    

12 Camera Lens Computar C 1Edmund Optics   90.00

13 Micro Rotary Stage .75" Rotary Stage 1Edmund Optics NT56-572 260.00

14 Aluminum Stock        

15 Fiber Optic Light Source   1 Dolan Jenner    

16 Grid Transparency   1 Output City -  

17 Linear Stage Optional for moving printed transparency 2 - 4Edmund Optics NT56-357 210.00

Software System Capabilities • Consist of three separate programs

• Kodak Molecular Imaging Software • Optigrid Motor Control GUI • MATLAB

– Scripts will be developed as necessary for calibration and depth resolved discrimination

• Optigrid Calibration– A system specific calibration process will be developed– Process will rely on manual coordination of three separate programs

• Implement necessary algorithms to achieve depth discrimination, as described by Tromberg et al. in US Publication 2006/0184043

Objective: To mathematically investigate and invoke the necessary

algorithms to achieve the depth discrimination, as described by Tromberg et al. in US Publication 2006/0184043

Bevilacqua, F., Cuccia, D., Durkin, A., Tromberg, B., “Quantitative analysis and imaging of subsurface heterogeneities using spatially structured illumination,” 2002.

Phantom Design• Objective: To develop a stable, long-term phantom whose optical

properties can be predetermined and reproduced, at selected wavelengths, of human biological tissues of the hand, over a spectral range between 400 and 1,000 nm.

• Purpose: – Simulate light distributions with a geometry of physical tissue.– Initially testing system designs – Calibration of optical devices.– Create reference measurements of reflective fluorescence.

• How:– Understand of the key physical and biochemical characteristics of

tissue that influence its interaction with light. – Match the absorption coefficient μa, scattering coefficient μs, and the

anisotropic coefficient g(λ)

Design of Phantom

• 3 components:– Casting material

• Polyurethane

– Absorbing material • phthalocyanin dye (Epolight 6084)

– Scattering agent • Aluminum Oxide (alumina; Al2O3)

Total attenuation coefficient, as a function of concentration, of 0.954-mm-diameter micro-spheres.