MOLD SAMPLING ANALYSIS REPORT - Cherokee …1).pdfMOLD SAMPLING ANALYSIS REPORT ... The purpose of the sampling was to determine if any unacceptable ... appendix A for Chain of Custody

Cherokee CRC, LLC

10838 E Marshall Street

Suite 220

Tulsa, Oklahoma 74116

MOLD SAMPLING ANALYSIS REPORT

For

Cherokee Nation Entertainment

Corporate Accounting Office

Tahlequah, OK

Introduction

Cherokee CRC, LLC, conducted a mold and asbestos sampling investigation in the Cherokee

Nation Entertainment Corporate accounting office located on Hwy 51 near Tahlequah, OK. The

sampling was conducted on December 19th

, 2014, January 16th

, 2015, and January 19th

, 2015.

Work was performed at the request of Joe Washum, Director of Safety and Environmental for

Cherokee Nation Businesses and Kevin Ogle, Director of Construction for Cherokee Nation

Businesses. The purpose of the sampling was to determine if any unacceptable concentrations of

mold spores exist in the sampled locations that could be negatively impacting employee health and

to determine if any materials in the complaint area contained asbestos containing materials.

Cherokee CRC, LLC coordinated with Mr. Washum and Matthew McCrary (CNE Safety and

Environmental) to conduct the sampling and to access the areas of investigation. The sampling

was conducted in a manner to minimize any disturbance of the mold colonies present inside the

building while providing maximum safety for project personnel and employees present during the

sampling.

Sampling Methodology

The initial sampling took place on December 19th

at the facility during a walkthrough with Kevin

Ogle and Joe Washum. The samples collected at this time included possible asbestos containing

materials within the building, more specifically, in the primary complaint area identified as Treva

Fields’ office. Five (5) samples were collected from suspect materials that could possibly contain

asbestos fibers. These samples included the complaint office’s wallboard around the window

sample 001, the baseboard and mastic sample 002, the carpet mastic 003, the brownish yellow attic

insulation present throughout the building, and a sample of the vermiculite from the wall cavity.

Two (2) tape lift mold samples were collected from the complaint area (Treva Fields’ Office) on

December 19th

. These samples included suspect white residue on the brick inner wall, and a rusty

red spot on the carpet at the base of the southern interior wall.

Additional mold sampling occurred on January 16th

, 2015. John Freise met with Mr. McCrary

who arranged access to the building for after-hours sampling as not to disturb employees who

working in the building. However, access was not available to the complaint area of the building

on the 16th

so only one (1) sample was collected which was a background sample collected on the

exterior of the building on the south side by the main entrance. The remaining sampling was

rescheduled to Monday January 19th

, 2015 when access to the complaint area could be secured.

On January 19th

, 2015 Mr. Freise again met with Mr. McCrary to collect the remaining mold

samples in the complaint area. Access was gained to the complaint area at approximately 1645

hours to begin the sampling. Three (3) samples were collect which included the following: NCA-

1 (hallway immediately north of the complaint area), CA-1 (Treva Fields’ office), and CAw-1 (the

interior wall taken from the outside southern exterior of the building).

Spore Trap sampling methodology was used for this sampling event. CCRC utilized a Zefon Bio-

pump Plus with Serial # 3014 which was pre calibrated to collect a 75 Liter sample of air from the

area of investigation. The samples were collected on Air-O-Cell Spore Trap Cassettes. Spore trap

analysis includes identification of all molds present within the air sample collected and also

quantification of spores per cubic meter. After collection, the spore trap samples were placed into a

plastic baggy. Tape lift sampling methodology was also utilized in this sampling event. Tape lift

samples collect a sample of spores from a solid surface. Tape lift samples include identification of

mold spores that may be growing on solid surfaces. All mold samples were sent to QuanTEM

Laboratories in Oklahoma City for analysis. The six (6) samples were collected at the following

times:

Sample ID Collection Date Collection Time Sample Type

TL-01 12/19/14 0945 hrs Tape Lift

TL-02 12/19/14 0945 hrs Tape Lift

BG-1 01/16/15 1700 hrs Spore Trap

NCA-1 01/19/15 1645 hrs Spore Trap

CA-1 01/19/15 1655 hrs Spore Trap

CAw-1 01/19/15 1715 hrs Spore Trap

Samples were sent to QuanTEM Laboratories and were received on January 22nd

, 2015(See

appendix A for Chain of Custody and Lab Report).

In addition to the spore trap sampling, CCRC staff also collected ambient air conditions within the

area of investigation. The ambient air conditions were collected using a Fluke 971 meter. This

meter will display the relative humidity in percent, air temperature in °F, and wet bulb dew point

in °F. The ambient air conditions recording within the areas of investigation are below:

Sample ID Air Temp (°F) Relative Humidity (%) Dew Point (°F)

CA-1 79.6 26.1 58.9

The asbestos samples were analyzed on December 23rd

, 2014 by Earth Tech Inc. using polarized

light microscopy using the McCrone method of dispersion staining analysis (See appendix B for

Lab Report).

Sample Analysis Summary

The mold samples were analyzed by QuanTem Laboratories in Oklahoma City, OK. The samples

were analyzed using Spore Trap, Quantitative NonCulturable, MM001 methodology and Tape

Lift, Qualitative NonCulturable, MM002 methodoly. QuanTem Laboratories is an American

Industrial Hygiene Association (AIHA) accredited laboratory. Their AIHA ID number is 101352

The results of the mold sampling at the Tahlequah warehouse indicate a substantial mold

concentration located in the complaint area (Treva Fields’ office). The air sampled contained an

80% coverage which indicates that the sampling cassette was overloaded with spores. As a result,

accurate quantification of the species of mold present was not possible. The overloaded cassette

does indicate that there is a significant concentration of mold spores present in the complaint area.

The sample collected in the non-complaint area (NCA-1) contained a small concentration of mold

spores that is significantly less the background sample (BG-1 collected outside). Sample CAw-1,

collected within the southernmost exterior wall, contained mold spores at a concentration slightly

larger than the background sample. (See Table 1 for a list of all detections)

Table 1.0 List of Detections (Results-Counts/m3)

Sample ID CA-1* NCA-1 BG-1 CAW-1 TL-01 TL-02

Alternaria Present 27 27

Ascospores Present 27 147 213 Few Few

Aspergillus/Penicillium Group 93 267 213 Few

Basidiospores Present 67 27

Bipolaris/Drechslera Group

(2)

Present Few

Cladosporium Present 67 253 680 Few

Epicoccum Present 27 80 13 Few

Hyphal Fragments Present 53 160 213

Periconia/Myxomycetes/Smuts Present 13 53 120 Few Few

Pithomyces/Ulocladium Present

Pollen 53

Rusts 13 13

Total Counts 293 1080 1559

Percent Coverage % 80 10 3 5

*Sample CA-1 sample cassette was overloaded quantification is not possible

The results of the asbestos sampling are as follows in Table 2.0:

Table 2.0 List of Asbestos Detections (% fibers)

Sample ID Color Asbestos Content Non Asbestos

Content

001-Wallboard Brown None Detected >95% Cellulose

002-Baseboard &

Mastic

Brown/Tan None Detected >95% Non-Fibrous

Material

003-Carpet Mastic Tan None Detected >95% Non-Fibrous

Material

004-Attic Insulation Brown None Detected 100% Cellulose

005-Wall Insulation,

Vermiculite

Brown/Black None Detected 3% Cellulose

97% Non-Fibrous

Material

(See appendix B for Lab Report)

Recommendations

Cherokee CRC staff visited the site and is familiar with the conditions present within the area-of-

investigation. The primary area of concern was identified to be the complaint area located in

Treva Fields’ office in the CNE Corporate Accounting office near Tahlequah, OK. The sample

collected within the complaint area (CA-1) was overloaded with mold spores. Due to these results

being much larger than the background sample, it would indicate active mold colonies are present

in this area (and in a much lesser case in the non-complaint area). Upon inspection of the

complaint area, it was immediately apparent that there is a moisture issue present due to water

infiltrating the southern interior wall and floor area around the wall. Water staining was visible on

the southern interior brick wall and floor area. The floor around the base of the wall shows signs

of water damage. At the time of sampling, the humidity in the room was at a normal range of

26.1%. No visible mold growth was present within the office. Additional destructive testing is

necessary to identify the source of water infiltration and mold growth. Since the mold results from

within the complaint area could not be quantified, the molds of most concern cannot be

determined. A list of mold species’ general characteristics is attached as appendix C. (See

appendix C for a list of characteristics of the identified mold Genera)

Appendix A

Microbiology Analytical Report (Results)

Date Received: 01/21/2015

Project: CNE Corporate Accounting

QuanTEM Lab ID: 245731

Analyzed By: Morgan Lane

Methodology: Tape, Qualitative NonCulturable, MM002

Location: Hwy 51, Talequah

Project No: 964

Account Number: B535

Received By: Patrick Mlekush

Date Analyzed: 01/22/2015

Client: Cherokee CRC

10838 E. Marshall St. Suite 220

Tulsa, OK 74116

AIHA ID Number: 101352

Results Results

245731-001 245731-002QuanTEM Sample ID

TL-01 TL-02Client Sample ID

Few FewAscospores

FewAspergillus/Penicillium Group

FewBipolaris/Drechslera Group (2)

FewCladosporium

FewEpicoccum

Few FewHyphal Fragments

Few FewPericonia/Myxomycetes/Smuts

Comments

Page 1 of 201/23/2015

Approved:

Morgan Lane, Analyst

Microbiology Analytical Report (Signature Page)

Unless otherwise noted, upon receipt the condition of the sample was acceptable for analysis.

(2)Also includes spores from Exosporium, Exserohilum and Helminthosporium

Few=10 or fewer fungal structures detected over area analyzed; Abundant=fungal structures detected in 75% or more of the

area analyzed or more than 500 fungal structures present; Moderate=fungal structure concentrations between few & abundant.

The results taken from your home, building, etc. cannot be interpreted without physical inspection of the contaminated area or

without considering the building’s characteristics and the factors that led to the present condition. Interpretation of results is

the responsibility of the company or individual who conducted the investigation.

This report shall not be reproduced except in full, without the written approval of the laboratory.

This report may not be used to claim endorsement by AIHA or any other agency of the U.S. Government





Methodology: Tape, Qualitative NonCulturable, MM002

Location: Hwy 51, Talequah

Project No: 964






Tulsa, OK 74116


Page 2 of 201/23/2015

Microbiology Analytical Report (Results)





Methodology: Spore Trap, Quantitative NonCulturable,

MM001 Location: Hwy 51, Talequah

Project No: 964






Tulsa, OK 74116


Results

Counts/m³

Results

Counts/m³

Results

Counts/m³

Results

Counts/m³

245731-003 245731-004 245731-005 245731-006QuanTEM Sample ID

CA-1 CAw-1 NCA-1 BG-1Client Sample ID

75 75 75 75Volume (L)

13 13 13 13Detection Limit

Present 27 27Alternaria

Present 213 27 147Ascospores

213 93 267Aspergillus/Penicillium Group

Present 27 67Basidiospores

PresentBipolaris/Drechslera Group (2)

Present 680 67 253Cladosporium

Present 13 27 80Epicoccum

Present 213 53 160Hyphal Fragments

13Nigrospora

Present 120 13 53Periconia/Myxomycetes/Smuts

PresentPithomyces/Ulocladium

53Pollen

13 13Rusts

XOverloaded; Mold Present

1559 293 1080Total Results (Counts/m³)

80 5 10 3Percent Coverage (%)

Overloaded;

Mold Present

Comments

Page 1 of 301/23/2015

Microbiology Analytical Report (Counts)







Project No: 964






Tulsa, OK 74116


Counts Counts Counts Counts

245731-003 245731-004 245731-005 245731-006QuanTEM Sample ID

CA-1 CAw-1 NCA-1 BG-1Client Sample ID

75 75 75 75Volume (L)

2 2Alternaria

16 2 11Ascospores

16 7 20Aspergillus/Penicillium Group

2 5Basidiospores

51 5 19Cladosporium

1 2 6Epicoccum

16 4 12Hyphal Fragments

1Nigrospora

9 1 4Periconia/Myxomycetes/Smuts

4Pollen

1 1Rusts

Page 2 of 301/23/2015

Approved:

Morgan Lane, Analyst

Microbiology Analytical Report (Signature Page)

Unless otherwise noted, upon receipt the condition of the sample was acceptable for analysis.

(2)Also includes spores from Exosporium, Exserohilum and Helminthosporium

Percent coverage = amount of particulate matter. With 25-50% coverage, results may be underestimated; with 50-70%

coverage, results will be underestimated; with >70% coverage, slides are designated overloaded (too dirty to count).

The results taken from your home, building, etc. cannot be interpreted without physical inspection of the contaminated area or

without considering the building’s characteristics and the factors that led to the present condition. Interpretation of results is

the responsibility of the company or individual who conducted the investigation.

This report shall not be reproduced except in full, without the written approval of the laboratory.

This report may not be used to claim endorsement by AIHA or any other agency of the U.S. Government.







Project No: 964






Tulsa, OK 74116


Page 3 of 301/23/2015

Appendix B

Earth Tech Enterprises, Inc.

3336 E. 32nd Street, Suite 234

Tulsa, OK 74135

918-712-9163

12/23/14

Cherokee CRC

John Freise

10838 E. Marshall St., Suite 220

Tulsa, OK 74116

Project Name: CWE Corporate Accounting Office

Sample No. Color Asbestos Non Asbestos Content

Content

________________________________________________________________________________

001 Brown None Detected >95% Cellulose

TR Office – Wallboard

002 Brown/Tan None Detected >95% Non Fibrous Material

TR Office – Baseboard & Mastic

003 Tan None Detected >95% Non Fibrous Material

TR Office Carpet Mastic

004 Brown None Detected 100% Cellulose

Attic – Brown Insulation

005 Brown None Detected 3% Cellulose

South Wall – Wall Insulation 97% Non Fibrous Material

Vermiculite, Black

I certify that the above samples were analyzed by Polarized Light Microscopy using the McCrone

method of dispersion staining analysis.

Appendix C

Documents

MOLD SAMPLING ANALYSIS REPORT - Cherokee …1).pdfMOLD SAMPLING ANALYSIS REPORT ... The purpose of the sampling was to determine if any unacceptable ... appendix A for Chain of Custody