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Micros 60• 3-part differential• Sample size= 10µL• Environment
– Indoor only– Level work station– Avoid direct exposure to sunlight– Proper ventilation– 60-90oF
• Linearity (AMR)– WBC (103/mm3): 0-100– RBC (106/mm3): 0-8.0– Hgb (g/dL): 2.0-27– Hct (%): 1.8-82.3– Plt (103/mm3): 25-4990
Parameters
Parameters• WBC• RBC• Hgb• Hct• MCV• MCH• MCHC• RDW• Platelet• MPV• Lym #• Lym%• Mono #• Mono%• Gra#• Gra%
Measurements/Calculations• WBC/RBC/PLT
– Impedance change• HGB
– Spectrophotometry• LYM%/MON%/GRA%
– Impedance change
Calculations• MCV,
MCH,MCHC,RDW,MPV,LYM#, MON#, GRA#
Stats
Principle of operation: The counting principle used for the red blood cells, white blood cells and platelets is based on the variations in impedance generated by the passage of cells through a calibrated aperture.
Number of samples which can be analyzed per hour: 60
Number of tests performed on this analyzer: 1
Quality control: Three levels run each day of patient testing, or following instrument repair, reagent lot change, or when indicated.
Calibration: Shift in control values, replacement of instrument parts, reagent number change, or when indicated
Preventative maintenance: Daily: start up and shut down, general cleaning
Acceptable samples: whole blood from an EDTA tube ran within 4 hours of collection
Limitations: Lipemia, cold agglutinins, high WBC’s
Equipement
• 8 Main parts– Electrical supply– Electronic board– Dilution pneumatics– Control panel with
keyboard and LCD screen– Reagent compartment– Printer– Smart card reader– Bar code reader (optional)
Reagents
– Diluent• ABX minidil: sodium hydroxide/azide• Determines and differenciates blood cells
– Cleaner• ABX miniclean: enzymatic detergent• Cleans system
– LYSE: potassium cyanide• ABX Alphalyse: Lyses RBCs, releasing hemoglobin and
WBCs. WBCs are counted by impedance.
• Waste
Daily Start Up1. Turn on instrument, once on, the start-up will begin automatically
1. Instrument is performing a cleaning and rinsing cycle: detergent in the chamber is rinsed with diluent
2. Cycle lasts about 130 seconds2. At cycle completion, background values will be displayed. These
values must be less than the below:1. WBC < 0.3 x 103/mm3
2. RBC < 0.02 x 106/mm3
3. Hgb < 0.0 g/dL4. Plt < 10x 103/ mm3
5. If the values are not within this range, another cycle will begin. If still not in range, the Micros will do another start-up. After 3 failed cycles, it will give a “start-up failed” message. Troubleshooting will now start.
Daily start-up con’t
3. Run a primer sample4. Perform QC and evaluate5. Complete maintenance sheet6. If all QC in, can continue with patient
samples.
Running a Patient
1. Press the ID button on the key pad
2. Manually enter the ID. Use the up and down arrows to enter alphabetical characters. Please Enter after each entry.