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Microscopy
Looking at the small stuff
Microscopes
“micro” means : Small
“scope” means: Watch/ look
It was actually developed first as entertainment for the rich
Light Microscope
Focuses visible light rays Can be used on live or dead specimens Dyes and fluorescent markers help for
viewing Magnify up to 1000X on the ones we have Examples:
Compound light microscope Confocal microscope
Light Microscope
Light Micrographs
Plant magnified
Human chromosomes
Confocal Microscope
Confocal micrograph
Spirogyra crassa
Maximum projectiongreen: Actin. Rhodamin, Label: Phalloidin. Actinred: Chloroplasts. AutofluorescenceImage acquired with the Leica TCS SP2
Electron Microscopy (EM) Focus beam of electrons on the
specimen Specimens must be dead Kinds
Transmission Electron Microscope (TEM) Cross-section of the material Most detailed of internal of cell
Scanning Electron Microscope (SEM) Surface of the specimen Shows texture, but less detail
TEM
TEM
SEM
SEM
Ant
Pollen Grains
Red Blood Cells
Compare cardiac myocytes
Maximum projection (overlay) of neonatal cardiac myocytes from rat.green: Myomesin, FITCred: Cadherin, Cy3blue: DNA (nuclei), DRAQ5Image acquired with the Leica TCS SL
Compare to TEM of myoctes on the wall.
Keen eyes
Light microscopes will not show you the nucleus of a cell or something the size of a virus!
Confocal microscopy uses brightly colored markers
TEM is detailed, flat, and black and white
SEM is 3D, textured, and black and white
Cited pictures Pollen grains (1000x magnification). Paul Fuqua. 2003.
unitedstreaming. 29 August 2006http://www.unitedstreaming.com/
Blood cells, red; microscopic view. U.S. Army. 2006.unitedstreaming. 29 August 2006http://www.unitedstreaming.com/
http://www-medlib.med.utah.edu/WebPath/HEMEHTML/HEME001.html
www.plymouth.ac.uk/upload/SCI/liverartery.jpg http://www.confocal-microscopy.com/WebSite/
pictures.nsf/(ALLIDs)/23C6531FD6B41E6BC1256A7D0053F8CA/$FILE/drosoegg_app_img_i_cl.jpg
http://www.confocal-microscopy.com/WebSite/pictures.nsf/(ALLIDs)/23C6531FD6B41E6BC1256A7D0053F8CA/$FILE/drosoegg_app_img_i_cl.jpg
Cited Pictures
http://remf.dartmouth.edu/images/insectPart3SEM/image/31formicidae40(extra).jpg
http://mikes-machine.mine.nu/Mysterymoss/Micro3_DSCN6486.jpg
http://www.nims.go.jp/analysis/TEM.jpg http://www.nims.go.jp/analysis/SEM.jpg http://cdr.rfmh.org/NewFiles/Components/
images/confocal%20microscope.jpg http://131.229.114.77/microscopy/images/
chromosomes2.jpg http://www.biosci.ohio-state.edu/~plantbio/
osu_pcmb/images/Courses/PB101/plant_cell_tem.jpg