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MicroscopyBy Jane Horlings
Microscopy
Robert Hooke, circa 1700s Electron Microscope, late 1900s
Early light microscope (UL), drawing by Hooke (LL)
Light Microscopes
Light Microscopes
Dissecting (stereo) microscope (L), compound microscope (R)
Light Microscopy; Plant Cells
Onion root tip, cell division (L), shoot tip (R)
Light Microscopy, Phase Contrast
Single celled Amoeba dividing (L), green alga Micrasterias (R)
Phase contrast
Fluorescence
Confocal
Light Microscopy
Electron Microscopes
Limitations of Electron Microscopy
• Works in a vacuum• Specimens are dead,
chemically preserved; no life processes can be seen
• No color (colorized by artist on computer)
Electron MicroscopesTransmission Electron Microscope (TEM)
Scanning Electron Microscope (SEM)
Scanning Electron Microscopy
SEM of cilia
TEM of cilia
Bacterium
RBCs in clot
SEM
Mite (UL)
Gecko toes (LL)
Shark skin (LR)
SEM
Red blood cells and Trypanosoma (L), Giardia (R)
SEMTrypanosome
Red blood cells
Transmission Electron Microscopy
SEM of cilia
TEM of cilia
Viruses (L), animal cell (R)
TEM
Animal cell (L), muscle tissue (R)
TEM
Light Microscopy
• Based on light• Specimens can be
alive; life processes can be seen
• Color; dyes may be used
Parts of the Microscope
• Ocular lenses
• Objective lenses
• How to compute the magnification
Use of the Microscope
• Place slide in center• Adjust light, lenses, barrel
– Put on low magnification!– Move stage all the way up
and then back down half a turn!
Use of the Microscope
• Look and readjust focus
• Move to higher magnification if needed
Use of the Microscope
• Importance of focusing with the fine adjustment!
• Oil immersion lens
• How to adjust the light
End