Microscopy & Imaging Shared Resource(MISR)Michael Johnson, Ph.D.MISR Director

Jan Blancato, Ph.D.Cytogenetics and immunofluorescence

Peter Johnson, B.S.Manager

Susette C. Mueller, Ph.D.Consultant

MISR Provides:

• Routine and advanced cellular imaging and image analysis for tumor and cell biology studies.

• Guidance for tumor biology studies using quantitative imaging: subcellular changes in protein distribution.

• Access to advanced imaging instrumentation hardware and image analysis software including Metamorph and Volocity.

• Cytogenetics resources and services such as DNA FISH.

MISR Services:

• TIRF– Fluorescence, single

molecule– 150 nm adjacent to

coverslip– Multicolor fluorescence at

the plasma membrane (only!)

– Applications:• Membrane receptors• Exocytosis, endocytosis• Cell adhesions (TIRF/IRM)

TIRF(Total Internal Reflection

Fluorescence microscopy)

MISR Services:

• Confocal/ Multiphoton– Single cell or deep

tissue (up to 500 mm maximum)

– Live cell imaging– Second harmonic

detection of collagen fibers

– FRET/FLIM for protein-protein study

Fluoview FV300

Zeiss LSM510/META/NLO

MISR Services:• Spinning disk

confocal (live) imaging– Fast, 3D confocal

imaging of cells– Volocity 2D and 3D

image analysis– Multiwell, automated

image collection

• Routine fluorescence

MISR Training:

• Training of users through lectures, workshops, and individual instruction.

• Some of the equipment can be used by trained users. Technical assistance can always be requested.

MISR Contacts

• Mike Johnson, PhD Core Director as of 7/1/10

– x7-0217 (W414 Res. Bldg.)

• Jan Blancato, PhD Cytogenetics

– x7-0217 (W414 Res. Bldg.)

• Peter Johnson, B.S. Manager

– x7-1916 (W407/W408 Res. Bldg.)

– pj42@georgetown.edu

• ZeissLSM and TIRF (E308 Res.Bldg)– x7-3168 (E308 Res. Bldg.)

Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01

  In your publications, remember to mention support provided from the GHUCCTS.